Evaluasi In Vivo Biokompatibilitas Implan Hidrogel Hidrokoloid Alami Untuk Aplikasi Biomedis
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Hidrogel merupakan material biomaterial yang menjanjikan untuk aplikasi biomedis karena sifat biokompatibilitasnya yang tinggi, struktur tiga dimensi yang menyerupai jaringan biologis, dan kemampuannya untuk terdegradasi secara bertahap dalam tubuh. Terutama, hidrogel hidrokoloid berbasis polimer alami, seperti glukomanan porang-sagu, menunjukkan potensi besar untuk aplikasi seperti sistem penghantaran obat dan rekayasa jaringan. Biokompatibilitas material ini sangat penting untuk memastikan bahwa ia dapat diterima dengan baik oleh jaringan tubuh tanpa menimbulkan reaksi merugikan. Oleh karena itu, penelitian ini bertujuan untuk mengevaluasi biokompatibilitas in vivo implan hidrogel hidrokoloid yang terbuat dari glukomanan porang-sagu yang dimodifikasi silang menggunakan asam sitrat, dengan fokus pada aplikasi biomedis. Metode yang digunakan dalam penelitian ini melibatkan implantasi implan hidrogel pada mencit jantan dengan berat sekitar 25 gram, yang dibagi menjadi empat kelompok: tiga kelompok dengan variasi formula implan hidrogel dan satu kelompok kontrol tanpa implan. Setelah 10-20 hari setelah implantasi, jaringan di sekitar implan dievaluasi melalui analisis histopatologi menggunakan pewarnaan Hematoksilin-Eosin (H&E). Hasil penelitian menunjukkan bahwa implan hidrogel memiliki biokompatibilitas yang baik, dengan respons inflamasi ringan yang didominasi oleh makrofag dan pembentukan kapsul fibrotik yang moderat. Tidak ditemukan tanda-tanda patologis seperti nekrosis atau granuloma pada jaringan sekitar implan. Formula F1 dan F5 menunjukkan biokompatibilitas yang lebih baik daripada F3, dengan respons inflamasi yang lebih rendah. Kesimpulannya, implan hidrogel hidrokoloid berbasis glukomanan porang-sagu menunjukkan potensi biokompatibilitas yang baik untuk aplikasi subkutan dan otot skelet. Namun, perlu dilakukan optimasi lebih lanjut untuk meningkatkan integrasi jaringan dan mengurangi celah antara implan dan jaringan sekitarnya. Penelitian ini memberikan dasar untuk pengembangan lebih lanjut material hidrogel hidrokoloid sebagai biomaterial untuk aplikasi biomedis, seperti sistem penghantaran obat dan rekayasa jaringan.
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