Determination Of Caffeine Rate Of Green And White Tea Leaf Extracts Camellia Sinensis (L.) With High Performance Liquid Chromatography
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Abstract
Tea, a widely popular and frequently consumed beverage, is integral to daily routines. Despite the potential long-term risks associated with excessive caffeine consumption, such as hypertension, heart disease, and stroke, caffeine is a prominent choice among a diverse spectrum of individuals in society. The objective of this research endeavour is to discern the caffeine content present in extracts derived from green and white tea leaves by applying the high-performance liquid chromatography (HPLC) method. The research procedure encompasses a series of stages, encompassing the preparation of botanical materials, extraction employing ethanol solvent, characteristic analysis, phytochemical screening, and quantification of caffeine levels within the green and white tea leaf extracts utilising the HPLC technique. The extraction process of green and white tea leaves entails maceration utilising 96% ethanol, followed by concentration via a rotary evaporator. The determination of caffeine concentrations is executed using the high-performance liquid chromatography method. A phytochemical screening analysis of ethanol extracts derived from green and white tea leaves unveiled a myriad of chemical compound classes, including alkaloids, flavonoids, saponins, tannins, and steroids. The quantification of caffeine content within the samples is ascertained by calculating the area under the curve (AUC) on the chromatograms of green and white tea extracts. The quantification results for caffeine content in the green tea extract reveal a value approximately equivalent to 98.4278069571 mg/g. The white tea extract demonstrates a content value of approximately 136.131488681 mg/g.
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References
Andry, Muhammad., Syufyani, Fahma., Muhammad, Amin Nasution., Ika, Julianti Tambunan., Muhammad, Faizal Fathurrohim & Firman, R. (2023). Skrining fitokimia dan Analisis Kadar Kafein Pada Kopi Bubuk Jenis Arabika Di Kota Takengon Menggunakan Spektrofotometri Ultraviolet. Journal Of Pharmaceutical And Sciences, 6(3), 998.
Angraini, N., & Desmaniar, P. (2020). Optimasi Penggunaan High Performance Liquid Chromatography (HPLC) Untuk Analisis Asam Askorbat Guna Menunjang Kegiatan Praktikum Bioteknologi Kelautan. Jurnal Penelitian Sains, 22(2), 69. https://doi.org/10.56064/jps.v22i2.583
Depkes RI. (1979). Materia Medica Indonesia (Jilid III). Dapartemen Kesehatan Republik Indonesia.
Depkes RI. (1995). Farmakope Indonesia. Edisi IV. Departemen Kesehatan Republik Indonesia.
Depkes RI. (2020). Farmakope Indonesia Edisi VI. Departemen Kesehatan Republik Indonesia.
Fadhilah, Z. H., Perdana, F., & Syamsudin, R. A. M. R. (2021). Review: Telaah Kandungan Senyawa Katekin dan Epigalokatekin Galat (EGCG) sebagai Antioksidan pada Berbagai Jenis Teh. Jurnal Pharmascience, 8(1), 31. https://doi.org/10.20527/jps.v8i1.9122
Harmono, H. D. (2020). Validasi Metode Analisis Logam Merkuri (Hg) Terlarutn pada Air Permukaan dengan Automatic Mercury Analyzer. Indonesian Journal of Laboratory, 2(3), 11. https://doi.org/10.22146/ijl.v2i3.57047
Ningrum, K. & M. M. (2013). Tumbuhan Sakti Basmi Berbagai Penyakit (Cetakan 1). Dunia Sehat.
Perbina, D. I., Ilsanna, C., Simorangkir, D. M., & Simarmata, H. (2021). Penetapan Kadar Kafein Pada Bubuk Teh Hitam Yang Beredar Di Pasar Deli Tua Menggunakan Spektrofotometri UV. 3(1).
Rohdiana, D. (2015). Teh : Proses, Karakteristik & Komponen Fungsionalnya. Universitas Al- Ghifari.
Siswanto, A., Fudholi, A., Nugroho, A. K., & Martono, S. (2016). Validasi Metode HPLC untuk Penetapan Aspirin dan Asam Salisilat dalam Plasma Kelinci ( Lepus curpaeums ) secara Simultan Validation of A High Performance Liquid Chromatography Method for The Simultaneous Determination of Aspirin and Salisylic Acid In Rabb. Jurnal Kefarmasian Indonesia, 6, 68–78.
Suprianto. (2018). Konsep Praktis High-Performance Liquid Chromatography. Institut Kesehatan Helvetia.
Thariq, N., Lc, M., Kimia, J. T., Teknik, F., Diponegoro, U., Sudharto, J. P., Fax, T., & Aryanti, P. N. (2010). Pembuatan Teh Rendah Kafein Melalui Proses Ekstraksi Dengan Pelarut Etil Asetat. 024, 8–10.
Verawati., Harun, S., Satria, B. (2014). Penetapan Kadar Konsumsi Kafein Dalam Minuman Teh Seduhan Yang Beredar Di Pasaran Secara KLT-Densitometri. Jurnal Scientia, 4(1).
Wardani, R. K., & Ferry Fernanda, M. A. H. (2016). Analisis Kadar Kafein Dari Serbuk Teh Hitam, Teh Hijau dan Teh Putih (Camellia sinensis L.). Journal of Pharmacy and Science, 1(1), 15–17. https://doi.org/10.53342/pharmasci.v1i1.48
Widhyani, R., Rahmasari, K. S., & Kristiyanti, R. (2021). Penetapan Kadar Kafein Pada Teh Kering Kemasan Produksi Industri Teh di Pekalongan. 1, 29–35.
Winata, H. S., Faisal, H., Andry, M., Aulia, N., Nasution, M. A., & Tambunan, I. J. (2023). Determination of total flavonoid content of ethanolic extract of yellow mangosteen (Garcinia xanthophylls) by spectrometry Uv-Vis method and LCMS. Journal of Pharmaceutical and Sciences, 6(3), 935–950.