Antibacterial activity test of the n-hexane and ethyl acetate fraction of kenanga leaves (Cananga odorata (Lam.) Hook. F. &Thomson) on Staphylococcus epidermidis
Main Article Content
Page: 73-79
Abstract
Human life always coexists with various types of microbes. One of them is kenaga leaves (Cananga odorata (Lam.) Hook. F. & Thomson), which is a plant that can be used as a traditional medicine, one of which can be used to treat itching on human skin and can inhibit bacteria, one of which is Staphylococcus epidermidis. This research aimed to determine the types of secondary metabolite compounds contained in ylang-ylang leaves (Cananga odorata (Lam.) Hook. F. & Thomson) and to test the antibacterial activity of the n-hexane and ethyl acetate fractions of ylang-ylang leaves (Cananga odorata (Lam.) Hook. F. & Thomson). This research method includes simplicia characterization and phytochemical screening on simplicia powder and 96% ethanol extract. Extraction was done by maceration with 96% ethanol solvent, then liquid-liquid fractionation with n-hexane and ethyl acetate solvents. The antibacterial activity test was carried out by measuring the diameter of the inhibition zone using the agar diffusion method. The results of the examination of the characteristics of ylang-ylang leaf Simplicia powder (Cananga odorata (Lam.) Hook. F. & Thomson) showed that the water content was 7.33%, the water-soluble essence content was 26.48%, the total ash was 3.52%, and acid insoluble ash content 0.18%. The results of phytochemical screening showed alkaloids, flavonoids, saponins, glycosides, tannins, and steroids. The activity test results on the n-hexane, ethyl acetate, and ethanol extract fractions had antibacterial activity. The n-hexane fraction gave strong results against Staphylococcus epidermidis bacteria at a 70 mg/ml concentration with an inhibitory zone diameter of 12.37 mm. The ethyl acetate fraction was strong against Staphylococcus epidermidis bacteria at a 70 mg/ml concentration with an inhibition zone diameter of 12.98 mm.
Downloads
Article Details
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
References
Afriani, R., Nasution, H.M., Mambang, D. E. P., & Dalimunthe, G. I. (2022). Uji Aktivitas Analgesik Ekstrak Daun Timun Tikus (Coccini Grandis(l). Voight) Terhadap Mencit Jantan (Mus Musculus). 1(April), 157-168.
Aulia.D, 2007. Pemeriksaan Penyaring Pada Kelainan Hemostasis. Dalam : Setiabudi RD, editor. Hemostasis dan Trambisis edisi 3. Fakultas Kedoteran Universitas Indonesia. Jakarta.
Caloprisco, E., Fourneron, J., Faure, R., & Demarne, F. (2001). Unusual lactones from cananga odorata (annonaceae). Journal of Agricultural and Food Chemistry, 50(1), 78-80. https://doi.org/10.1021/jf0105079
Indrakumar, I (2012). Evaluation Of Antimicrobial Antimicrobial Activity of Cananga odorata (Lam.) Hook. F. And Thomson Leaf Extract Leaf Extract: An In Vitro Study. Dept of Botany, Queen Mary’s College. Chennai.
Karlina, V.R., Nasution, H.M., Muslim, U., & Al, N. (2022). Skrining Fitokimia Dan Uji Aktivitas Antibakteri Ekstrak Etanol Daun Jeruk Purut (Citrus hystrix DC) Terhadap Bakteri Staphylococcus Aureus Dan Escherichia Coli 1(April), 131-139
Mambang, D. E. P., Nasution, H.M., & Friyani, L. (2021). Skrining Fitokimia Dan Penetapan Kadar Flavonoid Total Terhadap Ekstrak Etanol Sawi Pahit (Brassica juncea (L.) Czern) dengan metode spektrofotometri UV-VIS. Farmanesia, 8(2), 94-100
Munandar Nasution, H., Yuliarti, R., Rani, Z., & Nursyafira, A. (2022). Phytochemical Screening And Antibacterial Activity Test Of Ethanol Extract Of Jengkol Leaves (Archidendron Pauciflorum Benth.) I.C. Nielsen Against Staphylococcus Epidermidis And Propionibacterium Acnes. International Journal of Science, Technology & Management, 3(3), 647-653.
Munandar, A., Nasution, M. P., Nasution, H. M., & Mambang D. E. P. (2023). Phytochemical Screening And Cytotoxicity Testing Of Ethanol Extract Of Green Bean Sprout (Vigna Radiata (L.) Wilczek) With Method BSLT. Jurnal Farmasainkes, 2(2), 214-223.
Nasution, H. M. (2020). Skrining fitokimia dan isolasi senyawa steroid/triterpenoid dari ekstrak n-heksana rumput laut eucheuma alvarezii doty phytochemical screening and steroid/triterpenoid isolation of n-heksana extract of seaweed eucheuma alvarezii doty. Jurnal dunia farmasi, 4(3), 108-115
Nasution, H. M., Fatimah, C., & Syara, N. (2019). Karakterisasi simplisia skrining fitokimia dan uji toksisitas ekstrak etanol herbal binataro (cerbera manghas L.) terhadap artemia salina Leach characterization of phytochemical screening simplicia and toxicity test of herbal ethanol extract bintaro (Cerber. Farmenesia, 6(1), 19-26).
Nasution, H. M., Miswanda, D., & Dwiyani, A. O. (2022) Karakterisasi, skrining fitokimia dan uji aktivitas antiinflamasi ekstrak etanol daun dadap serep (Erythrina variegata Hassk.) terhadap tikus. Prosiding hasil seminar penelitian “Hilirisasi Penelitian Dan Pengabdian Kepada Masyarakat Menuju Universitas Internasional Yang Humanis, Mandiri Dan Islam, 107-112.
Priscilia, C., & Nasuition, H. M. (2022). Skrining fitokimia dan uji aktivitas antidiabetes ekstrak etanol daun bakung (Crinum asiaticum L.) pada mencit putih (Mus musculus) : farmasainkes: jurnal farmasi, sains, dan kesehatan, 1(2), 124-132
Sari, I. P., Wibowo, M. A.,dan Arreneuz, S. (2015). Aktivitas Antibakteri Ekstrak Teripang Butoh Keling (Holothuria Leucospilota) dari Pulau Lemukutan terhadap Bakteri Propionibacterium acnes dan Staphylococcus epidermidis. JKK, 4(4), 21–28.
Syafriana V, Hamida F, Damayanti R, dan Nanda EV. (2020). Aktivitas antibakteri ekstrak biji anggur (Vitis vinifera L.) terhadap Streptococcus pyogenes dan Streptococcus epidermidis. Sainstech Farma. Vol 13(1): 40-44.
Tan, L., Lee, L., Yin, W., Chan, C., Kadir, H., Chan, K., … & Goh, B. (2015). Traditional uses, phytochemistry, and bioactivities ofcananga odorata(ylang-ylang). Evidence-Based Complementary and Alternative Medicine, 2015, 1-30. https://doi.org/10.1155/2015/896314
Wei, A. and Shibamoto, T. (2007). Antioxidant activities and volatile constituents of various essential oils. Journal of Agricultural and Food Chemistry, 55(5), 1737-1742. https://doi.org/10.1021/jf062959x
Yang, Y., Isman, M., & Tak, J. (2020). Insecticidal activity of 28 essential oils and a commercial product containing cinnamomum cassia bark essential oil against sitophilus zeamais motschulsky. Insects, 11(8), 474. https://doi.org/10.3390/insects11080474
Zahara, S. L., Lubis, M. S., Dalimunthe, G. I., & Nasution, H. M. (2022). Aktivitas Antibakteri Ekstrak Etanol Lidah Buaya (Aloe Vera L.) Terhadap bakteri propionibacterium acnes. Journal of Health and Medical Science, 1(2), 157-168.