Uji efektivitas ekstrak etanol daun bayam hijau (Amaranthus hybridus L.) sebagai imunostimulan pada mencit putih jantan (Mus musculus)
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Sistem kekebalan tubuh memainkan peran penting dalam melindungi tubuh dari patogen, dan peningkatan aktivitasnya melalui imunostimulan sangat penting untuk meningkatkan kesehatan. Penelitian ini bertujuan untuk mengevaluasi potensi imunostimulasi dari ekstrak etanol daun bayam hijau (Amaranthus hybridus L.) pada mencit putih jantan (Mus musculus). Ekstrak etanol daun bayam hijau disiapkan dari daun amaranth hijau yang dikeringkan dan diuji untuk pengaruhnya terhadap aktivitas fagositosis menggunakan metode penghilangan karbon. Penyaringan fitokimia menunjukkan adanya flavonoid, saponin, tanin, dan steroid. Hewan percobaan dibagi menjadi lima kelompok: kontrol negatif (0,5% Na CMC), kontrol positif (Stimuno Forte®), dan tiga kelompok eksperimental yang menerima dosis ekstrak etanol yang berbeda (125 mg/kg, 250 mg/kg, dan 500 mg/kg berat badan). Hasil penelitian menunjukkan bahwa ekstrak etanol secara signifikan meningkatkan aktivitas fagositosis, dengan efek yang paling optimal diamati pada dosis 125 mg/kg berat badan. Indeks stimulasi meningkat dengan dosis yang lebih tinggi, menunjukkan efek imunostimulasi yang bergantung pada dosis. Penelitian ini menyimpulkan bahwa ekstrak etanol daun amaranth hijau dapat menjadi imunostimulan alami yang efektif, dengan dosis 125 mg/kg berat badan menjadi yang paling efektif dalam meningkatkan fungsi kekebalan tubuh pada tikus putih jantan. Temuan ini menunjukkan potensi amaranth hijau sebagai agen terapeutik untuk gangguan yang berkaitan dengan sistem kekebalan.
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