The Determination of Nicotine Content in Gayo Tobacco (Nicotiana tabacum L.) Using Acidimetric Titration and GC-MS Methods.
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Abstract
Background: Gayo tobacco (Nicotiana tabacum L.) is known for its distinctive quality and is highly sought after, particularly among young adults. This tobacco contains nicotine, a compound that plays a significant role in influencing human behavior, acting both as a stimulant and as a raw material for cigarette production. Understanding the nicotine content in Gayo tobacco is crucial for assessing its potential industrial applications and its impact on health. Objective: This study aimed to determine the nicotine content in Gayo tobacco leaves and analyze it using acidimetric titration and GC-MS methods to obtain more accurate results. Methods: This research employed two analytical methods: acidimetric titration for quantitative nicotine determination and Gas Chromatography-Mass Spectrometry (GC-MS) for qualitative analysis of other chemical compounds in the Gayo tobacco leaf extract. The tobacco leaf samples were obtained from the Gayo region of Aceh and extracted using 96% ethanol. Results: The study revealed that the nicotine content in Gayo tobacco leaves was 1,3% based on acidimetric titration, while the ethanol extract showed a higher nicotine concentration of 2.5%. GC-MS analysis identified various other compounds, including piperidine derivatives, terpenoids, and aromatic compounds, which have potential pharmaceutical and industrial applications. Conclusion: This study successfully determined the nicotine content in Gayo tobacco leaves using acidimetric titration and GC-MS. The findings provide a comprehensive overview of the chemical composition of Gayo tobacco, serving as a foundation for further research into its applications in pharmaceuticals and other industries.
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References
Daulay AS. Usaha produk minuman kesehatan jahe merah instan di kelurahan binjai kecamatan Medan Denai. Amaliah J Pengabdi Kpd Masy 2017;1:1–5. DOI: https://doi.org/10.32696/ajpkm.v1i1.7
Daulay AS, Nadia S, Daulay A. Eksplorasi kurkuminoid dari kunyit dan temulawak sebagai sediaan obat herbal. Pros. Semin. Nas. Has. Penelit., vol. 2, 2019, p. 454–61.
Supiyani, Miswanda D. Metabolite Sekunder Tembakau Gayo Isolasi dan Identifikasi. 1st ed. Medan: Penerbit LPPM UMNAW; 2024.
Supiyani S, Saisa S, Ningtias A. Phytochemichal identification of Gayo tobacco or bakong Gayo (Nicotiana tabacum L) and its potency for industrial applications. Justek J Sains Dan Teknol 2023;6:505–12.
Dewantara RD, Azis D. Evaluasi kesesuaian lahan perkebunan tembakau di kabupaten aceh tengah menggunakan analisis sistem informasi geografis. J Pendidik Geos 2021;6. DOI: https://doi.org/10.24815/jpg.v6i1.22099
Khasanah AU, Nastiti SJ. Identifikasi Senyawa Aktif Ekstrak Daun Tembakau (Nicotiana tabacum L.) Sebagai Antibakteri Terhadap S. aureus (ATCC 25923). Al-Hayat J Biol Appl Biol 2021;4:19–32. DOI: https://doi.org/10.21580/ah.v4i1.6320
Liem A. Pengaruh nikotin terhadap aktivitas dan fungsi otak serta hubungannya dengan gangguan psikologis pada pecandu rokok. Bul Psikol 2010;18.
World Health Organization W. Tobacco and Its Sedative Effects: A Technical Report. Geneva: 2021.
Supiyani S, Saisa S. GCMS Analysis of Bakong Gayo or Gayo Tobacco (Nicotiana tabacum L) Leaves Extract and Pharmaceutical Uses. Indones J Chem Sci Technol 2024;7:95. https://doi.org/10.24114/ijcst.v7i1.56451. DOI: https://doi.org/10.24114/ijcst.v7i1.56451
Alegantina S. Penetapan kadar nikotin dan karakteristik ekstrak daun tembakau (Nicotiana tabacum L.). J Penelit Dan Pengemb Pelayanan Kesehat 2017:113–9. DOI: https://doi.org/10.22435/jpppk.v1i2.509
Hossain AM, Salehuddin SM. Analytical determination of nicotine in tobacco leaves by gas chromatography–mass spectrometry. Arab J Chem 2013;6:275–8. DOI: https://doi.org/10.1016/j.arabjc.2010.10.006
Tao X, Zhang Y, Zhou Y, Liu Z, Feng X. Nicotine in complex samples: recent updates on the pretreatment and analysis method. Crit Rev Anal Chem 2023;53:1209–38. DOI: https://doi.org/10.1080/10408347.2021.2016365
Tumbel M. Analisis Kadar Nikotin dalam Tembakau Tongka Kabupaten Bantaeng (Analysis on The Concentrate of Nicotine in “ Tembakau Tongka ” of District Bantaeng). Bionature 2010;11:89–94. https://doi.org/https://doi.org/10.35580/bionature.v11i2.1383.
Taufik M, Wanto R, Athaillah A, Daulay AS, Siahaan LK, Ardilla D, et al. Analisis Nikotin Dalam Daun Tembakau Deli (Nicotiana tabacum L). J Stikna 2017;1:114–22.
Indonesia DK. Farmakope Indonesia Edisi III. 1979.
Friatna GN, Khumaisah LL, Anwar DI. Uji Efektivitas Sedatif-Hipnotik Ekstrak Daun Keji Beling (Strobilanthes crispa Blume) terhadap Mencit Putih Jantan. Pharm J Farm Indones (Pharmaceutical J Indones 2023;20:20–5. DOI: https://doi.org/10.30595/pharmacy.v0i0.14234
Dirjen POM. Farmakope Indonesia Edisi. IV. Jakarta: Depkes RI; 1995.
Bakkara LR. Uji Efektifitas Ekstrak Daun Tembakau dan Biji Mahoni Sebagai Insektisida Alami pada Tanaman Jambu Biji (Psidium guajava L.) 2014.
Wikardo BM. Formulasi sediaan hand sanitizer ekstrak tembakau (Nicotiana tabacum) dalam bentuk gel 2019.
Lestari P, Yusuf F, Fahdi F. Karakterisasi Simplisia Dan Skrining Fitokimia Herba Suruhan (Peperomia pellucida H.B.&K.). Best J (Biology Educ Sains Technol 2023;6:990–6. https://doi.org/https://doi.org/10.30743/best.v6i2.9699.
Habib MA, Islam MM, Islam MM, Hasan MM, Baek K-H. Current status and de novo synthesis of anti-tumor alkaloids in Nicotiana. Metabolites 2023;13:623. DOI: https://doi.org/10.3390/metabo13050623
Khafid A, Wiraputra MD, Putra AC, Khoirunnisa N. Uji Kualitatif Metabolit Sekunder pada Beberapa Tanaman yang Berkhasiat sebagai Obat Tradisional. Bul Anat Dan Fisiol 2023;8:61–70. DOI: https://doi.org/10.14710/baf.8.1.2023.61-70
Li L, Zhang H, Wen J, Shen Y, Li D, Luo C, et al. Direct determination of free nicotine content in tobacco. ACS Omega 2022;7:23061–8. DOI: https://doi.org/10.1021/acsomega.2c00089
Nguyen HN, Ullevig SL, Short JD, Wang L, Ahn YJ, Asmis R. Ursolic acid and related analogues: triterpenoids with broad health benefits. Antioxidants 2021;10:1161. DOI: https://doi.org/10.3390/antiox10081161
Wolff M. Principle-Principle of Medicinal Chemistry. Fourth Ed Gajah Mada Press Yogyakarta 1994.
Ningsi A. Aktivitas Antioksidan Dari Ekstrak Daun Tembakau (Nicotiana Tabacum L.) Yang Berasal Dari Desa Cabbenge Kabupaten Soppeng. Makassar Univ Negeri Islam Alauddin 2018.
Hermawati AH, Pratiwi CD, ST S, Lathifah QA. Nikotin, Tembakau, Dan Rokok. Penerbit Andi; 2023.
Erian FO, Muarif A, Ginting Z, Zulnazri Z. Pemanfaatan Ekstrak Nikotin dari Limbah Puntung Rokok menjadi Insektisida. J Teknol Kim Unimal 2022;11:258–66. DOI: https://doi.org/10.29103/jtku.v11i2.9465
Fauziyah N, Widyasanti A, Sutresna Y. Kajian pengaruh konsentrasi etanol terhadap karakteristik oleoresin ampas jahe merah (Zingiber officinale Roscoe) limbah penyulingan. TEKNOTAN 2022;16:169. DOI: https://doi.org/10.24198/jt.vol16n3.6
Daulay AS, Nadia S. Eksplorasi Ekstrak Kurkuminoid Rimpang Kunyit Dengan Perbandingan Metode Maserasi Dan Pelarut Berdasarkan Aktivitas Antioksidan. Pros. Semin. Nas. Has. Penelit., vol. 2, 2019, p. 1722–8.
Lubis MS, Rani Z, Wahyuni W, Arlian RY. Test of sunscreen activity of pineapple weevil ethanol extract (ananas comosus (L.) merr.) in gel and lotion preparations. AMCA J Sci Technol 2023;3:7–12. DOI: https://doi.org/10.51773/ajst.v3i1.196
Davis DL, Nielsen MT. Tobacco: production, chemistry and technology 1999.
Herlina N, Azizah N, Pradiga EP. Pengaruh Suhu dan Curah Hujan terhadap Produktivitas Tembakau (Nicotiana tabacum L.) di Kabupaten Malang The Effect of Temperature and Rainfall on Tobacco (Nicotiana tabacum L.) Productivity in Malang Regency n.d.
Gonzalez-Rivera ML, Barragan-Galvez JC, Gasca-Martínez D, Hidalgo-Figueroa S, Isiordia-Espinoza M, Alonso-Castro AJ. In vivo neuropharmacological effects of neophytadiene. Molecules 2023;28:3457. DOI: https://doi.org/10.3390/molecules28083457