The determination of the total flavonoid content of yellow wood extracts, from Samarkilang, Central Aceh with various concentrations ethanol using visible spectrophotometry method
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Abstract
The yellow wood sampled was taken from the Samarkilang area of Central Aceh. The use of yellow wood plants as herbal medicinal ingredients. The chemical compounds contained in Yellow Wood are alkaloids, phenolics, flavonoids, saponins, and tannins. Phenolic compounds and flavonoids have activity as antioxidants. The purpose of this study was to determine the chemical compounds contained in the ethanol extract and to determine the total flavanoid value of the ethanol extract of the yellow wood (Arcangelisia flava (L.) Merr). This research method is an experimental method which includes processing plant materials, making 50%, 70%, and 96% ethanol extracts. Examination of characterization, phytochemical screening and determination of total flavonoid content of the ethanol extract of Kayu kuning using the Uv-Vis spectrophotometry method. Yellow wood extract was prepared by maceration method using various ethanol concentrations of 50%, 70% and 96% the extract obtained was concentrated with a rotary evaporator. Furthermore, the determination of total flavonoid levels was carried out using the Uv-Vis spectrophotometry method. The results of the phytochemical screening on the ethanol extract of Kayu kuning contained groups of chemical compounds such as alkaloids, flavonoids, saponins, tannins, and steroids/triterpenoids. Determination of total flavonoid content was carried out by determining the maximum wavelength of quercetin, operating time, measuring the quercetin calibration curve and calculating total flavonoid content using the Uv-Vis spectrophotometry method. The results of determining total flavanoid levels in ethanol solvent extract with a concentration of 50% 0,96882 ± 0,2157 mgQE/g, in ethanol solvent extract with a concentration of 70% was 2,6693 ± 11,7041 mgQE/g, and in ethanol solvent extract with a concentration of 96 % is 3,838 ± 1,3036 mgQE/g.
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References
Aminah., Tomahayu & Abidin, Z. (2017). Penetapan Kadar Flavonoid Total Ekstrak Etanol Kulit Buah Alpukat (Persea Americana Mill) Dengan Metode Spektrofotometri Visible. Jurnal Fitofarmaka Indonesia. Vol 4, No.2 .
Day. R. A., Underwood. A. L. (1999). Analisa Kimia Kuantitatif. Edisi keempat. Erlangga: Jakarta.
Depkes RI. (1979). Farmakope Edisi III. Jakarta: Departemen Kesehatan RI. Hal: 31, 330, 763, 766, 649, 659.
Depkes RI. (1995). Farmakope Indonesia. Edisi IV. Jakarta: Departemen Kesehatan RI. Hal.672-1033.
Dewi, W, F., Mahrunisa., Dhea Febrina Kipli. 2019. Skrining Fitokimia dan penetapan kandungan senyawa flavonoid ekstrak etanol kulit buah Jeruk Gerga dengan metode Spektrofotometri Visible. Jurnal Ilmiah Farmacy. Akademi Farmasi Al-Fatah Bengkulu. Vol,6 No.2.
Diliarosta, S., Efendi, A., Dillasamola, D., Oktomalioputri, B., & Ramadhani, R. (2021). Reconstruction and scientific explanation of akar kuning (arcangelisia flava merr.) from west sumatra as ethnomedicine and source of science learning. Pharmacognosy Journal, 13(1), 206-211. https://doi.org/10.5530/pj.2021.13.29
Ditjen POM. (1979). Materi Medika Indonesia Jilid III. Jakarta: Direktorat Jenderal Pengawasan Obat dan Makanan. Hal. 155-161.
Ginovyan, M., Petrosyan, M., & Trchоunian, A. (2017). Antimicrobial activity of some plant materials used in armenian traditional medicine. BMC Complementary and Alternative Medicine, 17(1). https://doi.org/10.1186/s12906-017-1573-y
Guerriero, G., Berni, R., Muñoz-Sánchez, J., Apone, F., Abdel-Salam, E., Qahtan, A., … & Hernández‐Sotomayor, S. (2018). Production of plant secondary metabolites: examples, tips and suggestions for biotechnologists. Genes, 9(6), 309. https://doi.org/10.3390/genes9060309
Harborne, J., B. 1987. Metode Fitokimia: Penuntun Cara Modern Menganalisa Tumbuhan Terbitan Kedua. Terjemahan Kosasih Padmawinata dan Iwang Soediro. ITB: Bandung.
Kemenkes RI. (2020). Materia Medika Indonesia. Jilid IV. 177-180. Kementrian Kesehatan Republik Indonesia. Jakarta. Hal: 48.Sahidin, I. (2012). Mengenal Senyawa Alami Pembentukan dan Pengelompokan Secara Kimia. Kendari: Unhalu Press.
Pramudita, Riwanti, Farizah Izazih, Amaliyah. 2020.Pengaruh Perbedaan Konsentrasi Etanol pada Kadar Flavonoid Total Ekstrak Etanol 50,70 dan 96% Sargassum polycystum dari Madura. Journal of Pharmaceutical Care Anwar Medika, 02 : 82-95.
Pratama, M. (2017). Akar kuning (arcangelisia flava) as neuraminidase inhibitor: molecular docking and pharmacophore optimization approach. https://doi.org/10.2991/smichs-17.2017.63
Pratama, M., Suratno, S., & Mulyani, E. (2018). Profile of thin-layer chromatography and uv-vis spectrophotometry of akar kuning stem extract (arcangelisia flava). Borneo Journal of Pharmacy, 1(2), 72-76. https://doi.org/10.33084/bjop.v1i2.367
Ramadani, A., Paloque, L., Belda, H., Tamhid, H., Jumina, J., Augereau, J., … & Benoit-Vical, F. (2018). Antiprotozoal properties of indonesian medicinal plant extracts. Journal of Herbal Medicine, 11, 46-52. https://doi.org/10.1016/j.hermed.2017.06.004
Ramadhan, A., Baroroh, R., Maharany, D., Ulfa, E., & Puspitasari, E. (2022). Cardioprotective effect of chloroform extract of arcangelisia flava on doxorubicin-induced cardiomyopathy. Jurnal Ilmu Dasar, 23(1), 43. https://doi.org/10.19184/jid.v23i1.23012
Sahidin, I. (2012). Mengenal Senyawa Alami Pembentukan dan Pengelompokan Secara Kimia. Kendari: Unhalu Press.
Sari, A, K., Riza, A., Siska, M., Prasdianto., Renny. 2018. Penetapan Kadar Fenolok Dan Flavonoid Total Ekstrak Metanol Kayu Kuning (Arcangelisia flava Merr) Dengan Metode Spektrofotometri Visible. Jurnal Insan Farmasi Indonesia. Akademik Farmasi ISFI Banjarmasin. Vol 1. No (2).
Subandrate, S., Athiah, M., Romadhon, M., & Tariza, N. (2022). Xanthine oxidase inhibitory activity of arcangelisia flava. Acta Biochimica Indonesiana, 5(1), 71. https://doi.org/10.32889/actabioina.71
subeki, s., Matsuura, H., Takahashi, K., Yamasaki, M., Yamato, O., Maede, Y., … & Yoshihara, T. (2005). Antibabesial activity of protoberberine alkaloids and 20-hydroxyecdysone from arcangelisia flava against babesia gibsoni in culture. Journal of Veterinary Medical Science, 67(2), 223-227. https://doi.org/10.1292/jvms.67.223
Wang, W., Su, M., Li, H., Zeng, B., Chang, Q., & Zhang, L. (2018). Effects of supplemental lighting with different light qualities on growth and secondary metabolite content ofanoectochilus roxburghii. Peerj, 6, e5274. https://doi.org/10.7717/peerj.5274
Wulansari, D., Jamal, Y., & Agusta, A. (2014). Pachybasin, a major metabolite from culture broth of endophytic coelomyceteous afkr-18 fungus isolated from a yellow moonsheed plant, arcangelisia flava (l.) merr.. Hayati Journal of Biosciences, 21(2), 95-100. https://doi.org/10.4308/hjb.21.2.95
Zhao, L., Xu, Y., Lai, X., Shan, C., Deng, Z., & Ji, Y. (2015). Screening and characterization of endophytic bacillus and paenibacillus strains from medicinal plant lonicera japonica for use as potential plant growth promoters. Brazilian Journal of Microbiology, 46(4), 977-989. https://doi.org/10.1590/s1517-838246420140024.