Determination of flavonoid levels of raru bark methanol extract (Cotylelobium lanceolatum Craib) on variations in solvent concentration using UV-Vis spectrophotometry method
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Abstract
The use of plant-based traditional medicine remains a common practice in developing countries, with more than 80% of the population relying on it for healthcare and treatment. In Indonesia, natural medicines continue to be a primary choice for public health, aligning with local traditions and cultural practices. One such medicinal plant is raru bark, which possesses various pharmacological activities, including antidiabetic, astringent, antilaxative, antibacterial, antiseptic, antidiarrheal, and blood-clotting properties. This study aims to characterize the methanol extract of raru bark and determine its flavonoid content using UV-Vis spectrophotometry. The research stages included plant material processing, methanol extraction, characterization tests, macroscopic and microscopic examinations, phytochemical screening, and flavonoid quantification. Phytochemical analysis revealed that the methanol extract of raru bark contains flavonoids, tannins, saponins, and triterpenoids. Characterization tests showed that all evaluated parameters met the required standards. Flavonoid quantification was performed by determining the maximum wavelength of quercetin using UV-Vis spectrophotometry at different solvent concentrations. The results indicated that the total flavonoid content at a 96% solvent concentration was 1.625 ± 0.153 mgQE/g, at 70% was 1.318 ± 0.003 mgQE/g, and at 50% was 1.146 ± 0.006 mgQE/g.
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References
Fauziah F, Maghfirah L, Hardiana. Gambaran Penggunaan Obat Tradisional Pada Masyarakat Desa Pulo Secara Swamedikasi. J Sains Dan Kesehat Darussalam 2021;1:13. https://doi.org/10.56690/jskd.v1i1.11. DOI: https://doi.org/10.56690/jskd.v1i1.11
Yuziani Y, Rianza MA, Sofia R. Gambaran Pengetahuan Obat Tradisional Di Kalangan Masyarakat Desa Juli Mee Teungoh Kecamatan Juli Kabupaten Bireuen. Galen J Kedokt Dan Kesehat Mhs Malikussaleh 2023;2:1. https://doi.org/10.29103/jkkmm.v2i4.12228. DOI: https://doi.org/10.29103/jkkmm.v2i4.12228
Hadiq S, Sirajuddin W. Evaluasi Penggunaan Obat Tradisional Berdasarkan Dimensi Ketepatan Cara Penggunaan. J Farm IKIFA 2024;3:83–94.
Oktarlina RZ, Carolia N. Hubungan pengetahuan keluarga dengan penggunaan obat tradisional di desa nunggalrejo kecamatan punggur kabupaten lampung tengah. Jk Unila J Kedokt Univ Lampung 2018;2:42–5.
Adiyasa MR, Meiyanti M. Pemanfaatan obat tradisional di Indonesia: distribusi dan faktor demografis yang berpengaruh. J Biomedika Dan Kesehat 2021;4:130–8. DOI: https://doi.org/10.18051/JBiomedKes.2021.v4.130-138
Pasaribu G. Aktivitas Inhibisi Alfa-Glukosidase pada beberapa Jenis Kulit Kayu Raru. J Penelit Has Hutan 2011;29:10–9. DOI: https://doi.org/10.20886/jphh.2011.29.1.10-19
Pasaribu GT. Zat ekstraktif kayu raru dan pengaruhnya terhadap penurun kadar gula darah secara in vitro 2009.
Latifah L. Identifikasi golongan senyawa flavonoid dan uji aktivitas antioksidan pada ekstrak rimpang kencur Kaemferia galanga L. dengan metode dpph (1, 1-difenil-2-pikrilhidrazil) 2015.
Neldawati N. Analisis nilai absorbansi dalam penentuan kadar flavonoid untuk berbagai jenis daun tanaman obat. Pillar Phys 2013;2.
Uddin J, Shah SWA, Zahoor M, Ullah R, Alotaibi A. Chalcones: The flavonoid derivatives synthesis, characterization, their antioxidant and in vitro/in vivo antidiabetic potentials. Heliyon 2023;9. DOI: https://doi.org/10.1016/j.heliyon.2023.e22546
Zheng X, Zhang L, Wang W, Wu Y, Zhang Q, Feng W. Anti-diabetic activity and potential mechanism of total flavonoids of Selaginella tamariscina (Beauv.) Spring in rats induced by high fat diet and low dose STZ. J Ethnopharmacol 2011;137:662–8. DOI: https://doi.org/10.1016/j.jep.2011.06.018
Adawiyah R. Uji Aktivitas Antihiperlipidemia Ekstrak Etanol Daun Kalakai (Stenochlaena Palutris (Burm. F.) Bedd Pada Tikus Putih Wistar Secara In vivo. Lumbung Farm J Ilmu Kefarmasian 2023;4:87–93.
Rahman AA, Kurniati NF, Sukandar EY. Ekstrak Daun Binahong Mencegah Kenaikan Kolesterol Darah pada Tikus yang Diberi Pakan Lemak Tinggi. J Farm Indones Vol 2016;8:150.
Nurhayati R. Antihyperglychemic activity test of extract, methanol fraction and n-heksan fraction of ketapang leaf (Terminalia catappa) in alloxan-induced white rats. J Wiyata Penelit Sains Dan Kesehat 2023;10:35–45. DOI: https://doi.org/10.56710/wiyata.v10i1.609
Sjahid LR. Isolasi dan identifikasi flavonoid dari daun dewandaru (Eugenia uniflora L.) 2008.
Yeti A, Yuniarti R. Penetapan Kadar Flavonoid Total Ekstrak Etanol Herba Rumput Bambu (Lopatherum gracile Brongn.) dengan Metode Spektrofotometri Visible. FARMASAINKES J Farm Sains, Dan Kesehat 2021;1:11–9. DOI: https://doi.org/10.32696/fjfsk.v1i1.812
Afrizani A, Daulay AS, Ridwanto R, Rahman F. Penetapan kadar flavonoid total ekstrak kayu kuning dari daerah Samarkilang Aceh Tengah dengan berbagai konsentrasi etanol menggunakan metode spektrofotometri visible. J Pharm Sci 2023:80–90. https://doi.org/10.36490/journal-jps.com.v6i5-si.391. DOI: https://doi.org/10.36490/journal-jps.com.v6i5-si.391
Mirna M, Nasution MA, Ridwanto R, Daulay AS. Penetapan Kadar Flavonoid Total Ekstrak Etanol Dan Ekstrak Etil Asetat Daun Pala (Myristica fragrans Houtt) Secara Spektrofotometri Visible. Forte J 2024;4:134–42. DOI: https://doi.org/10.51771/fj.v4i1.762
Natasya E, Daulay AS, Ridwanto R, Rahayu YP. Penetapan kadar flavonoid total ekstrak kayu raru (Cotylelobium lanceolatum Craib) berdasarkan perbedaan konsentrasi etanol dengan metode spektrofotometri Uv-Vis. J Pharm Sci 2023;6:1804–10. https://doi.org/10.36490/journal-jps.com.v6i4.305. DOI: https://doi.org/10.36490/journal-jps.com.v6i4.305
Rossa A, Daulay AS, Ridwanto R, Rahayu YP. Aktivitas antioksidan dan uji toksisitas ekstrak kulit batang kayu raru (Cotylelobium lanceolatum Craib) dengan menggunkan metode DPPH dan metode BSLT. J Pharm Sci 2023:339–52. https://doi.org/10.36490/journal-jps.com.v6i5-si.436. DOI: https://doi.org/10.36490/journal-jps.com.v6i5-si.436
Afni L, Daulay AS, Ridwanto R, Rahayu YP. Penetapan kadar fenolik total ekstrak kayu raru (Cotylelobium lanceolatum Craib) berdasarkan perbedaan konsentrasi etanol dengan metode spektrofotometri UV-Vis. J Pharm Sci 2023:1811–8. https://doi.org/10.36490/journal-jps.com.v6i4.306. DOI: https://doi.org/10.36490/journal-jps.com.v6i4.306
Iswanto AH, TARIGAN FO, Susilowati A, Darwis A, Fatrıasarı W. Wood Chemical Compositions of Raru Species Originating From Central Tapanuli, North Sumatra, Indonesia: Effect of Differences in Wood Species and Log Positions. J Korean Wood Sci Technol 2021;49:416–29. https://doi.org/10.5658/wood.2021.49.5.416. DOI: https://doi.org/10.5658/WOOD.2021.49.5.416
Depkes RI J. Farmakope Indonesia Edisi III 1979.
Depkes RI. Materia Medika (Indonesia Medical Materials). 1989.
Depkes RI. Farmakope Indonesia Edisi IV. Jakarta: Departemen Kesehatan Republik Indonesia; 1995.
Wendersteyt NV, Wewengkang DS, Abdullah SS. Uji Aktivitas Antimikroba Dari Ekstrak Dan Fraksi Ascidian Herdmania momus Dari Perairan Pulau Bangka Likupang Terhadap Pertumbuhan Mikroba Staphylococcus aureus, Salmonella typhimurium Dan Candida albicans. Pharmacon 2021;10:706. https://doi.org/10.35799/pha.10.2021.32758. DOI: https://doi.org/10.35799/pha.10.2021.32758
Sondari D, Puspitasari ED. Teknologi Ekstraksi Fluida Superkritis Dan Maserasi Pada Zingiber Officinalle Roscoe : Aktivitas Antioksidan Dan Kandungan Fitokimia. J Sains Mater Indones 2018;18:74. https://doi.org/10.17146/jsmi.2017.18.2.4168. DOI: https://doi.org/10.17146/jsmi.2017.18.2.4168
Wijaya H, Jubaidah S, Rukayyah R. Perbandingan Metode Esktraksi Terhadap Rendemen Ekstrak Batang Turi (Sesbania Grandiflora L.) Dengan Menggunakan Metode Maserasi Dan Sokhletasi. Indones J Pharm Nat Prod 2022;5:1–11. https://doi.org/10.35473/ijpnp.v5i1.1469. DOI: https://doi.org/10.35473/ijpnp.v5i1.1469
Ratu AP, Himawan HC, Radhi MR. Uji antioksidan ekstrak air dan ekstrak etanol daging dan kulit buah blewah (Cucumis melo L.). J Farmamedika (Pharmamedika Journal) 2017;2:1–6. DOI: https://doi.org/10.47219/ath.v2i1.24
Dewatisari WF. Perbandingan pelarut kloroform dan etanol terhadap rendemen ekstrak daun lidah mertua (Sansevieria trifasciata. Prain) menggunakan metode maserasi. Pros. Semin. Nas. Biol., vol. 6, 2020, p. 127–32.
Kiswandono AA. Skrining senyawa kimia dan pengaruh metode maserasi dan refluks pada biji kelor (Moringa Oleifera, Lamk) terhadap rendemen ekstrak yang dihasilkan. J Sains Nat 2017;1:126. https://doi.org/10.31938/jsn.v1i2.21. DOI: https://doi.org/10.31938/jsn.v1i2.21
Widwiastuti H, Asworo RY, Tjahjaningsih YS, Wulandari NC, Dewi A. Pengaruh Ukuran Simplisia Dan Lama Kontak Pada Ekstraksi Senyawa Aktif Simplisia Kayu Jawa (Lannea Coromandelica) Menggunakan Metode Maserasi. J Kim Mulawarman 2022;19:86. https://doi.org/10.30872/jkm.v19i2.1141. DOI: https://doi.org/10.30872/jkm.v19i2.1141
Yikwa N, Supriyanto S, Hidayati D. Perbandingan Metode Ekstraksi Konvensional, Pengepresan, Dan Enzimatis Terhadap Kualitas Minyak Buah Merah (Pandanus Conoidues). J Rekayasa Dan Manaj Agroindustri 2025;12:600. https://doi.org/10.24843/jrma.2024.v12.i04.p09. DOI: https://doi.org/10.24843/JRMA.2024.v12.i04.p09
Sembiring BB, Bermawie N, Rizal M, Kartikawati A. Pengaruh Teknik Ekstraksi Daun Ubi Jalar Ungu (Ipomoea Batatas) Dan Daun Jambu Biji (Psidium Guajava) Terhadap Aktivitas Antioksidan. J Jamu Indones 2020;5:22–32. https://doi.org/10.29244/jji.v5i1.184. DOI: https://doi.org/10.29244/jji.v5i1.110
Puspitasari AD, Wulandari RL. Aktivitas Antioksidan Dan Penetapan Kadar Flavonoid Total Ekstrak Etil Asetat Daun Kersen (Muntingia Calabura). J Pharmascience 2017;4. https://doi.org/10.20527/jps.v4i2.5770. DOI: https://doi.org/10.20527/jps.v4i2.5770
Yuniarto K, Muvianto CMO, Ernia E. Aplikasi Ultrasound Assisted Extraction Untuk Produksi Minyak Bawang Putih Varietas Lokal. J Teknol Pertan 2021;22:177–86. https://doi.org/10.21776/ub.jtp.2021.022.03.3. DOI: https://doi.org/10.21776/ub.jtp.2021.022.03.3
Sulistyarini I, Sari DA, Wicaksono TA. Skrining Fitokimia Senyawa Metabolit Sekunder Batang Buah Naga (Hylocereus polyrhizus). Cendekia Eksakta 2020;5.
Farnsworth NR. Biological and phytochemical screening of plants. J Pharm Sci 1966;55:225–76. https://doi.org/10.1002/jps.2600550302. DOI: https://doi.org/10.1002/jps.2600550302
Sangi M, Runtuwene MRJ, Simbala HEI, Makang VMA. Analisis fitokimia tumbuhan obat di Kabupaten Minahasa Utara. Chem Prog 2019;1:47–53.
Sibi G, Ramesh N, Dhananjaya K, Ravikumar KR, Mallesha H. Potential Use of Muntingia Calabura L. Extracts Against Human and Plant Pathogens. Pharmacogn J 2012;4:44–7. https://doi.org/10.5530/pj.2012.34.8. DOI: https://doi.org/10.5530/pj.2012.34.8
Yoshida T, Amakura Y, Yoshimura M. Structural Features and Biological Properties of Ellagitannins in Some Plant Families of the Order Myrtales. Int J Mol Sci 2010;11:79–106. https://doi.org/10.3390/ijms11010079. DOI: https://doi.org/10.3390/ijms11010079
Demain AL. REVIEWS: The business of biotechnology. Ind Biotechnol 2007;3:269–83. https://doi.org/10.1089/ind.2007.3.269. DOI: https://doi.org/10.1089/ind.2007.3.269
Mulyani S, Laksana T. Analisis flavonoid dan tannin dengan metoda mikroskopi-mikrokimiawi. Maj Obat Tradis 2011;16:109–14.
Robinson T. The organic constituents of higher plants: their chemistry and interrelationships. Burgess Life Sci Ser 1963. DOI: https://doi.org/10.5962/bhl.title.6010
Mabry T, Markham KR, Thomas MB. The systematic identification of flavonoids. Springer Science & Business Media; 2012.
Ruhardi A, Sahumena MH. Identifikasi senyawa flavanoid daun sembung (Blumea Balsamifera L.). J Syifa Sci Clin Res 2021;3:29–36. https://doi.org/10.37311/jsscr.v3i1.9925. DOI: https://doi.org/10.37311/jsscr.v3i1.9925
Hasanah N, Novian DR. Analisis Ekstrak Etanol Buah Labu Kuning (Cucurbita Moschata D.). Parapemikir J Ilm Farm 2020;9:54. https://doi.org/10.30591/pjif.v9i1.1758. DOI: https://doi.org/10.30591/pjif.v9i1.1758
Segara Y, Kurniawan A. Uji Aktivitas Antioksidan dan Penetapan Kadar flavonoid Total pada Ekstrak Etanol Daun Iler (Coleus scutellarioides (L.) Benth.). J Farm Dan Farmakoinformatika 2023;1:60–75. DOI: https://doi.org/10.35760/jff.2023.v1i1.8070
Widyasari EM, Sriyani ME, Daruwati I, Halimah I, Nuraeni W. Karakteristik fisikokimia senyawa bertanda 99mTc-kuersetin. J Sains Dan Teknol Nukl Indones 2019;20:9. https://doi.org/10.17146/jstni.2019.1.1.4108. DOI: https://doi.org/10.17146/jstni.2019.1.1.4108
Solikah WY, Fatmawati A, Gunawan A, Defri AY. Uji Kualitatif Dan Penetapan Kadar Flavonoid Total Ekstrak Etanol Herba Pegagan (Centella asiatica) Dengan Variasi Konsentrasi Pelarut. J Pharm Sci 2023;6:673–80. https://doi.org/10.36490/journal-jps.com.v6i2.89. DOI: https://doi.org/10.36490/journal-jps.com.v6i2.89
Islamiyati R, Saputri IN. Uji perbedaan aktivitas antioksidan dengan variasi konsentrasi pelarut etanol 70% dan 96% pada ekstrak etanol daun salam menggunakan metode peredaman radikal bebas DPPH. Cendekia J Pharm 2018;2:134–42. https://doi.org/10.31596/cjp.v2i2.28. DOI: https://doi.org/10.31596/cjp.v2i2.28
Kusuma P. Penetapan kadar flavonoid total dan daya antioksidan dari ekstrak etanol buah pare (momordica charantia l). Skripsi Tidak Diterbitkan Makassar UIN Alaudin 2012.
Khoirunnisa I, Sumiwi SA. Review Artikel: Peran Flavonoid Pada Berbagai Aktifitas Farmakologi 2019.
Vifta RL, Saputra YD, Hakim AL. Analisis Flavonoid Total Ekstrak Buah Parijoto (Medinilla Speciosa) Asal Bandungan Dan Formulasinya Dalam Sedian Gel. J Exp Clin Pharm 2022;2:21. https://doi.org/10.52365/jecp.v2i1.342. DOI: https://doi.org/10.52365/jecp.v2i1.342
Dewi CE, Saleh C, Pratiwi DR, Magdaleni AR, Daniel D. Potensi aktivitas antioksidan ekstrak metanol daun singkil (Premna corymbosa Roxb & Willd.). J At 2024;9:137–44.
Rifanti E. Aktivitas antioksidan ekstrak metanol dari bekatul beras merah (Oryza nivara L.) berdasarkan pada suhu dan waktu maserasi 2023.
Yuliani H, Rasyid MI. Efek Perbedaan Pelarut terhadap Uji Toksisitas Ekstrak Pineung Nyen Teusalee. J Fitofarmaka Indones 2019;6:347–52. DOI: https://doi.org/10.33096/jffi.v6i2.453