Evaluation of analgesic and antidiarrheal activity of Clitoria ternatea Linn. ethanol flower extract
Article Sidebar
Main Article Content
Page: 358-364
Abstract
The analgesic and antidiarrheal effects of Clitoria ternatea Linn. flower extract were tested in mice. A significant analgesic response (p<0.05) was observed in acetic acid-induced pain models in a dose-dependent manner. The percent protection of analgesic properties was calculated from the reduction of writhing by butterfly pea ethanol extract. The Butterfly pea extract doses at 100, 200, and 400 mg/ kg showed analgesic protection by 76.6% (9.4 ± 3.65), 93.9% (2.4 ± 1.95), and 97.5% (1.0 ± 0.71), respectively. The antidiarrheal properties of Clitoria ternatea Linn. flower extract were assessed using the castor oil-induced diarrhea method. Clitoria ternatea Linn. flower extract delayed the onset of castor oil-induced diarrhea in a dose-dependent manner and reduced the frequency of defecation. There was no significant difference (p>0.05) between the effects of 400 mg/kg of Clitoria ternatea Linn. flower extract and loperamide in protecting mice against castor oil-induced diarrhea. Based on the study results, it is suggested that the Clitoria ternatea Linn. flower extract has strong analgesic and antidiarrheal properties.
Downloads
Article Details
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
References
Suarna W, Wijaya MS. Butterfly pea (clitoria ternatea L.: Fabaceae) and its morphological variations in Bali. J Trop Biodivers Biotechnol 2021;6:1–12. https://doi.org/10.22146/JTBB.63013. DOI: https://doi.org/10.22146/jtbb.63013
Oguis GK, Gilding EK, Jackson MA, Craik DJ. Butterfly pea (Clitoria ternatea), a cyclotide-bearing plant with applications in agriculture and medicine. Front Plant Sci 2019;10:1–23. https://doi.org/10.3389/fpls.2019.00645. DOI: https://doi.org/10.3389/fpls.2019.00645
Jeyaraj EJ, Lim YY, Choo WS. Extraction methods of butterfly pea (Clitoria ternatea) flower and biological activities of its phytochemicals. J Food Sci Technol 2021;58:2054–67. https://doi.org/10.1007/s13197-020-04745-3. DOI: https://doi.org/10.1007/s13197-020-04745-3
Widowati W, Wargasetia TL, Zakaria TM, Marthania M, Akbar RATPP, Gunadi MS, et al. Antioxidant Activity of TEMON (Clitoria ternatea and Citrus sp.) as an Infused Herbal Tea. Maj Obat Tradis 2022;27:32–40. https://doi.org/10.22146/mot.71628. DOI: https://doi.org/10.22146/mot.71628
Widowati W, Darsono L, Lucianus J, Setiabudi E, Susang Obeng S, Stefani S, et al. Butterfly pea flower (Clitoria ternatea L.) extract displayed antidiabetic effect through antioxidant, anti-inflammatory, lower hepatic GSK-3β, and pancreatic glycogen on Diabetes Mellitus and dyslipidemia rat. J King Saud Univ - Sci 2023;35:102579. https://doi.org/10.1016/j.jksus.2023.102579. DOI: https://doi.org/10.1016/j.jksus.2023.102579
Pandhare R, Balakrishnan S, Bangar G, Dighe P, Deshmukh V. Antidiarrheal activity of Clitorea ternatea Linn. (Fabaceae) ethanol leaf extract in rats. AYU (An Int Q J Res Ayurveda) 2018;39:40. https://doi.org/10.4103/ayu.ayu_19_17. DOI: https://doi.org/10.4103/ayu.AYU_19_17
Ferraz CR, Carvalho TT, Manchope MF, Artero NA, Rasquel-Oliveira FS, Fattori V, et al. Therapeutic potential of flavonoids in pain and inflammation: Mechanisms of action, pre-clinical and clinical data, and pharmaceutical development. vol. 25. 2020. https://doi.org/10.3390/molecules25030762. DOI: https://doi.org/10.3390/molecules25030762
Tan B, Wu X, Yu J, Chen Z. The Role of Saponins in the Treatment of Neuropathic Pain. Molecules 2022;27:1–21. https://doi.org/10.3390/molecules27123956. DOI: https://doi.org/10.3390/molecules27123956
Del Prado-Audelo ML, Cortés H, Caballero-Florán IH, González-Torres M, Escutia-Guadarrama L, Bernal-Chávez SA, et al. Therapeutic Applications of Terpenes on Inflammatory Diseases. Front Pharmacol 2021;12:1–7. https://doi.org/10.3389/fphar.2021.704197. DOI: https://doi.org/10.3389/fphar.2021.704197
Rawat P, Singh PK, Kumar V. Evidence based traditional anti-diarrheal medicinal plants and their phytocompounds. Biomed Pharmacother 2017;96:1453–64. https://doi.org/10.1016/j.biopha.2017.11.147. DOI: https://doi.org/10.1016/j.biopha.2017.11.147
Fatmawati A, Bachri MS, Nurani LH. Combination Effects of Moringa oleifera Leaf Ethanol Extract and Andrographis paniculata Herb on Blood Glucose Levels and Pancreas Histopathology of Diabetic Rats Induced by Streptozotocin. Maj Obat Tradis 2019;24:85. https://doi.org/10.22146/mot.39401. DOI: https://doi.org/10.22146/mot.39401
Harborne JB, Sudiro I, Padmawinata K, Niksolihin S. Metode fitokimia : penuntun cara modern menganalisis tumbuhan. Bandung: Penerbit ITB; 1996.
Solikah WY, Fatmawati A, Gunawan A, Defri AY. Uji Kualitatif Dan Penetapan Kadar Flavonoid Total Ekstrak Etanol Herba Pegagan (Centella asiatica) Dengan Variasi Konsentrasi Pelarut. J Pharm Sci 2023;6:673–80. https://doi.org/10.36490/journal-jps.com.v6i2.89. DOI: https://doi.org/10.36490/journal-jps.com.v6i2.89
Md Idris MH, Mohd Amin SN, Mohd Amin SN, Nyokat N, Khong HY, Selvaraj M, et al. Flavonoids as dual inhibitors of cyclooxygenase-2 (COX-2) and 5-lipoxygenase (5-LOX): molecular docking and in vitro studies. Beni-Suef Univ J Basic Appl Sci 2022;11. https://doi.org/10.1186/s43088-022-00296-y. DOI: https://doi.org/10.1186/s43088-022-00296-y
GUAN F, WANG H, SHAN Y, CHEN Y, WANG M, WANG Q, et al. Inhibition of COX-2 and PGE2 in LPS-stimulated RAW264.7 cells by lonimacranthoide VI, a chlorogenic acid ester saponin. Biomed Reports 2014;2:760–4. https://doi.org/10.3892/br.2014.314. DOI: https://doi.org/10.3892/br.2014.314
Bamidele Okoli J. Diterpenoids of Alepidea Amatymbica Eckl. & Zeyh: Studies of Their Cytotoxic, Antimicrobial, and Lipoxygenase Inhibitory Activities. MOJ Bioorganic Org Chem 2017;1:103–11. https://doi.org/10.15406/mojboc.2017.01.00019. DOI: https://doi.org/10.15406/mojboc.2017.01.00019
Jing W, Xiaolan C, Yu C, Feng Q, Haifeng Y. Pharmacological effects and mechanisms of tannic acid. Biomed Pharmacother 2022;154:113561. https://doi.org/10.1016/j.biopha.2022.113561. DOI: https://doi.org/10.1016/j.biopha.2022.113561
Toemon AN, Carmelita AB, Widiarti A, Mutiasari D. Pengaruh Pemberian Ekstrak Etanol Buah Masisin (Rhodomyrtus tomentosa Wight) terhadap Frekuensi, Konsistensi, dan Durasi Diare pada Hewan Coba Mencit Putih Jantan (Mus musculus) yang Diinduksi Oleum Ricini. J Surya Med 2019;5:22–40. https://doi.org/10.33084/jsm.v5i1.943. DOI: https://doi.org/10.33084/jsm.v5i1.943
Fauzi R, Fatmawati A. Efek Anti diare Ekstrak Etanol Daun Kelor ( Moringa oleifera L . ) Pada Mencit Putih Jantan Antidiarrheal Effect of Ethanol Extract of Moringa Leaves ( Moringa oleifera L .) in Male Mice 2020;6:35–9. DOI: https://doi.org/10.21776/ub.pji.2020.006.01.6