Antioxidant activity and toxicity of raru (Cotylelobium lanceolatum Craib) bark extract using the DPPH method and BSLT method
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Abstract
Raru (Corylelobium lanceolatum Craib) is a group of endemic tropical forest plants from the Dipterocarpaceae family in Indonesia. Raru is a term for a group of types of bark added to palm sap, which aims to increase the taste and alcohol content of the drink. People in Sumatra believe that raru bark (Cotylelobium lanceolatum Craib) can be used as an antidiabetic drug. This study aims to determine the antioxidant activity of raru bark, the toxicity activity of raru bark extract, and the secondary metabolites in raru bark. This research was conducted in descriptive was born with a descriptive method. The sample used was raru bark. The research phase included sample preparation, plant identification, simplicial manufacture, antioxidant activity test, DPPH standard stock preparation, blank solution preparation, phytochemical screening, antioxidant activity test based on IC50, Toxicity test, and toxicity activity test based on LC50 value. The result obtained in testing the antioxidant activity of raru bark had an IC50 value of 94.25 µg/mL in the vary strong category. While the result of the probit analysis for the toxicity of raru bark extract using the BSLT method obtained an LC50 value of 350.9942 µg/mL. It is in the toxic category and has the potential to be an anticancer.
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References
Afni, L. (2023). Penetapan kadar fenolik total ekstrak kayu raru (cotylelobium lanceolatum craib) berdasarkan perbedaan konsentrasi etanol dengan metode spektrofotometri uv-vis. Journal of Pharmaceutical and Sciences, 1811-1818. https://doi.org/10.36490/journal-jps.com.v6i4.306
Agustini, N. W. S. (2013). Aktivitas Antioksidan Dan Uji Toksisitas Hayati Pigmen Fikobiliprotein Dari Ekstrak Spirulina Platensis. Journal Bioteknologi, 9(1): 107-110.
Angelina, M., P. Amelia., M. Irsyad., L. Meilawati., dan M. Hanafi. (2015). Karakterisasi Ekstrak Etanol Herba Katumpangan Air (Peperomia pellucida L. Kunth). Biopropal Industri. Jakarta: UIN Syarif Hidayatullah. 6(2) 53-61.
Aprilliani, R., S.P. Fitrianingsih., dan R. Choesrina. (2016). Standardisasi Mutu Simplisia dan Ekstrak Metanol Daun Paitan (Tithonia diversifolia (Hemsley) A. Gray). Prosiding Farmasi. Bandung: Universitas Bandung. 2(2): 286-292.
Dechandt, C., Souza, D., Siqueira, J., Pereira, M., Assis, R., Silva, V., … & Baviera, A. (2014). Changes in the oxidative stress biomarkers in liver of streptozotocin-diabetic rats treated with combretum lanceolatum flowers extract. British Journal of Pharmaceutical Research, 4(20), 2340-2356. https://doi.org/10.9734/bjpr/2014/13558
Depkes RI. (1989). Materia Medika Indonesia Jilid V. Depkes RI: Jakarta.
Depkes RI. (1995). Materia Medika Indonesia Jilid VI.Depkes RI: Jakarta. Hal: 357-361.
Depkes RI. (1995). Materia Medika Indonesia. Jilid keenam. Jakarta: Departemen Kesehatan Republik Indonesia. Hal. 299-305, 334-335.
Ditjen POM. (1989). Materia Medika Indonesia. Jilid kelima. Jakarta: Departemen Kesehatan Republik Indonesia. Hal. 169-171.
Hasan, A. H. (2021). Skrining Fitokimia dan Uji Aktivitas Antioksidan Jantung Pisang Goroho (musa acuminafe l.) Dengan Metode 1, 1-diphenyl-2-picrylhidrazyl (DPPH). Indonesian Journal of Pharmaceutical Education. 1(3). 136-141.
Hidayat, S. (2021). Sweet wooden stem leather (cinnamomum burmanii) assessed from effective extraction and dosage methods for diabetes (literature review). Symbiotic Journal of Biological Education and Science, 2(2), 40-54. https://doi.org/10.32939/symbiotic.v2i2.19
Kartikasari, D., Nurkhassanah., dan S. Pramono. (2015). Karakterisasi Simplisia dan Ekstrak Etanol Daun Bertoni (Stevia rebaudiana) Dari Tiga Tempat Tumbuh. Jurnal Ilmu Farmasi dan Farmasi klinik. Semarang: Universitas Wahid Hasyim.
Maqsood, S. and Benjakul, S. (2011). Effect of kiam (cotylelobium lanceolatum craib) wood extract on the haemoglobin-mediated lipid oxidation of washed asian sea bass mince. Food and Bioprocess Technology, 6(1), 61-72. https://doi.org/10.1007/s11947-011-0530-x
Marjoni, R. (2016). Dasar – Dasar Fitokimia Untuk Diploma III Farmasi. Jakarta: Trans Info Media.
Meyer, B.N., Ferrigni, N.R., Putnam, J.E., Jacobsen, L.B., Nichols, D.E., dan McLaughin, J.L. (1982). Brine Shrimp: A Convenient General Bioassay for Active Plant Constituent. Planta Medica. 45:31-34.
Molyneux P. (2004). The use of the stable free radical diphenylpicrylhydrazyl (DPPH) for estimating antioxidant activity. Songklanakarin Journal of Science Technology. 26 (2): 211-219.
Natasya, E. (2023). Penetapan kadar flavonoid total ekstrak kayu raru (cotylelobium lanceolatum craib) berdasarkan perbedaan konsentrasi etanol dengan metode spektrofotometri uv-vis. Journal of Pharmaceutical and Sciences, 6(4), 1804-1810. https://doi.org/10.36490/journal-jps.com.v6i4.305
Panggabean, L., Nurhamidah., dan D. Handayani. (2020). Profil Fitokimia dan Uji Sitotoksik Ekstrak Etanol Tumbuhan zanthoxylum acanthopodium dc (Andaliman) Menggunakan Metode BSLT. Jurnal Pendidikan dan Ilmu Kimia. Bengkulu: Universitas Bengkulu. 4(1): 59-68.
Pasaribu, G., & Setyawati, T. (2011). Aktivitas antioksidan dan toksisitas ekstrak kulit kayu raru (Cotylelobium sp.). Jurnal Penelitian Hasil Hutan, 29(4), 322-330.
Silalahi, J. (2006). Makanan Fungsional. Yogyakarta: Kanisius.
Sitepu, N.B., dan Rini. B. (2019). Uji Toksisitas Ekstrak Etanol Kulit Batang Cepcepan (Castanopsis costata BL) Dengan Metode Brine Shrimp Lethality Test (BSLT). Borneo Journal Of Phamascientech. Medan: Poltekkes Kemenkes. 3(1): 20-27.
Subekti, N.K. (2014). Uji Toksisitas Akut Ekstrak Metanol Daun Laban Abang (Aglaia elliptica Blume) Terhadap Larva Udang (Artemia Salina Leach) Dengan Metode Brine Shrimp Lethality Test (BSLT). Skripsi. Jakarta: UIN Syarif Hidayatullah.
Sugianti, N. (2007). Brine Shrimp Lethality Test Ekstrak Etanol Daun Tumbuhan Temblekan (Lantana camara L.) Beserta Profil Kromatografi Lapis Tipis. Skripsi. Yogyakarta: Universitas Sanata Dharma.
Suharyanto, S., dan Prima, D. A. N. (2020). Penetapan Kadar Flavonoid Total pada Juice Daun Ubi Jalar Ungu (Ipomoea Batatas L.) yang Berpotensi Sebagai Hepatoprotektor dengan Metode Spektrofotometri UV-Vis. Cendekia Journal of Pharmacy. 4(2). 110-119.
Syukur, R., Alam, G., Mufidah, A. R., & Tayeb, R. (2011). Aktivitas Antiradikal Bebas Beberapa Ekstrak Tanaman Familia Fabaceae. JST. Kesehatan, 1(1), 1411-1674.
Verawati, N. and Aida, N. (2017). Uji aktivitas antibakteri terhadap bakteri patogen dan identifikasi senyawa aktif ekstrak kulit kayu raru (vatica leucocapra). Jurnal Pertanian, 8(2), 82. https://doi.org/10.30997/jp.v8i2.1059
Wahid, A. R., dan Safwan. (2019). Skrining Fitokimia Senyawa Metabolit Sekunder Terhadap Ekstrak Tanaman Ranting Patah Tulang (Euphorbia tiruculli L.). Jurnal Ulul Albab, 23(1): 45-47.
Winahyu, D. A., Retnaningsih, A., & Aprillia, M. (2019). Penetapan kadar flavonoid pada kulit batang kayu raru (CotylelobiummelanoxylonP) dengan metode spektrofotometri uv-vis. Jurnal Analis Farmasi, 4(1).
Yang, Z., Xin, Z., Wang, L., & Xia, M. (2023). Antimycobacterial compound of cynoglossum lanceolatum forsk.: bioassay guided isolation, molecular docking, synthesis of analogs, and a plausible mechanism of action. Chemistry & Biodiversity, 20(2). https://doi.org/10.1002/cbdv.202200965
Yuda, P., Mahardika, I., Cahyaningsih, E., Sasadara, M., Nayaka, N., & Dewi, N. (2022). Aktivitas anti-inflamasi minyak herbal tradisional dari bahan usada bali pada mencit inflamasi yang diinduksi karagenan. JPSCR Journal of Pharmaceutical Science and Clinical Research, 7(3), 319. https://doi.org/10.20961/jpscr.v7i3.60529.