Eksplorasi Aktivitas Antimikroba Bakteri Endofit Daun Betadine (Jatropha multifida L.) terhadap Patogen Staphylococcus aureus
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Latar Belakang: Resistensi antibiotik mendorong pencarian senyawa antibakteri baru, termasuk dari bakteri endofit tanaman obat seperti Jatropha multifida L. (Daun Betadine) yang secara tradisional digunakan sebagai antiseptik. Tujuan: Mengevaluasi potensi antibakteri isolat bakteri endofit Daun Betadine terhadap Staphylococcus aureus. Metode: Penelitian eksperimental laboratorium menggunakan metode difusi cakram kertas. Isolat endofit dikultur dalam media Nutrient Broth, kemudian supernatan disentrifugasi dan diuji aktivitasnya dengan membandingkan zona hambat terhadap kontrol positif (kloramfenikol) dan negatif (Na.CMC). Hasil: Isolat bakteri endofit menghasilkan zona hambat 16,11 mm (kategori sedang), sementara kloramfenikol 27,63 mm (kategori kuat). Kontrol negatif tidak menunjukkan zona hambat. Kesimpulan: Bakteri endofit Daun Betadine berpotensi sebagai agen antibakteri alami, meskipun efektivitasnya lebih rendah dibanding antibiotik standar. Potensi ini perlu dikembangkan melalui identifikasi senyawa aktif dan uji toksisitas lebih lanjut.
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