Pengaruh gel ekstrak induk kunyit (Curcuma longa linn) terhadap penyembuhan luka sayat pada tikus putih jantan (Rattus norvegicus)
Bilah Samping Artikel
Isi Artikel Utama
Page: 616-627
Abstrak
Salah satu tanaman yang telah terbukti membantu penyembuhan luka adalah kunyit. Kunyit telah terbukti secara ilmiah memiliki sifat koleretik dan anti-inflamasi. Kunyit mengandung bahan kimia yang disebut kurkumin, yang memiliki kualitas antibakteri dan antioksidan yang dapat mempercepat migrasi dan re-epitelisasi sel-sel yang berguna dalam penyembuhan luka, termasuk myofibroblas, fibroblas, dan makrofag. Tujuan dari penelitian ini adalah untuk mengetahui pengaruh gel ekstrak induk kunyit terhadap penyembuhan luka pada tikus putih jantan (Rattus norvegicus). Penelitian ini dilakukan dengan menggunakan uji eksperimental. Pembuatan ekstrak kunyit dilakukan dengan menggunakan pelarut etanol 96% dengan proses maserasi. Luka sayatan sepanjang 2 cm dibuat pada punggung tikus putih. Hewan uji kemudian dibagi menjadi lima kelompok untuk mendapatkan perlakuan yang berbeda. Kelompok 3, 4, dan 5 mendapatkan gel ekstrak induk kunyit dengan konsentrasi 1%, 5%, dan 10%, sedangkan kelompok 1 sebagai kontrol negatif dan kelompok 2 sebagai kontrol positif. Hasil penelitian menunjukkan bahwa luka sayat pada tikus putih jantan dapat sembuh lebih cepat - rata-rata sekitar 7,10 hari - ketika diberi perlakuan dengan ekstrak etanol 96% induk kunyit dengan dosis 1%, 5%, dan 10%. Kesimpulan dari penelitian ini adalah bahwa metode terbaik untuk mempercepat penyembuhan luka adalah dengan menggunakan gel yang mengandung ekstrak induk kunyit 10%.
Unduhan
Rincian Artikel
Artikel ini berlisensiCreative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
Referensi
M. Falcone et al., ‘Challenges in managing chronic wound infections,’ J. Glob. Antimicrob. Resist., vol. 26, pp. 140–147, 2021. DOI: https://doi.org/10.1016/j.jgar.2021.05.010
V. E. Kaban, J. O. Aritonang, Y. C. Hasibuan, and D. I. P. Meliala, ‘Efektivitas Penyembuhan Luka Sayat Menggunakan Salep Ekstrak Etanol Daun Senggani (Melastoma Malabathricum L.) Pada Kelinci’, J. Penelit. Farm. Herb., vol. 2, no. 2, pp. 8–14, 2020. DOI: https://doi.org/10.36656/jpfh.v2i2.207
L. Marinelli et al., ‘In vitro wound-healing properties of water-soluble terpenoids loaded on halloysite clay,’ Pharmaceutics, vol. 13, no. 8, p. 1117, 2021. DOI: https://doi.org/10.3390/pharmaceutics13081117
N. Nasri, V. E. Kaban, D. Satria, H. D. Syahputra, and Z. Rani, ‘Mekanisme Antibakteri Ekstrak Etanol Daun Kemangi (Ocimum basilicum L.) terhadap Salmonella typhi,’ J. Pharm. Health Res., vol. 4, no. 1, pp. 79–84, 2023. DOI: https://doi.org/10.47065/jharma.v4i1.3176
M. T. Carvalho, H. G. Araújo-Filho, A. S. Barreto, L. J. Quintans-Júnior, J. S. Quintans, and R. S. Barreto, ‘Wound healing properties of flavonoids: A systematic review highlighting the mechanisms of action,’ Phytomedicine, vol. 90, p. 153636, 2021. DOI: https://doi.org/10.1016/j.phymed.2021.153636
E. S. Rumaseuw et al., Farmakognosi. Global Eksekutif Teknologi, 2023.
A. C. da Silva, P. D. de Freitas Santos, J. T. do Prado Silva, F. V. Leimann, L. Bracht, and O. H. Goncalves, ‘Impact of curcumin nanoformulation on its antimicrobial activity,’ Trends Food Sci. Technol., vol. 72, pp. 74–82, 2018. DOI: https://doi.org/10.1016/j.tifs.2017.12.004
N. Suhartatik, M. Karyantina, and C. E. Setyaningsih, ‘The Antimicrobial Potency of White Turmeric (Curcuma ceasia) in Breadfruit Starch (Artocarpus altilis) Edible Film,’ Food Sci. J. Food Sci. Technol., vol. 2, no. 1, pp. 73–84, 2022. DOI: https://doi.org/10.33830/fsj.v2i1.2948.2022
D. Akbik, M. Ghadiri, W. Chrzanowski, and R. Rohanizadeh, ‘Curcumin as a wound healing agent,’ Life Sci., vol. 116, no. 1, pp. 1–7, 2014. DOI: https://doi.org/10.1016/j.lfs.2014.08.016
X. He et al., ‘Curcumin-Loaded Mesenchymal Stem Cell-Derived Exosomes Efficiently Attenuate Proliferation and Inflammatory Response in Rheumatoid Arthritis Fibroblast-Like Synoviocytes,’ Appl. Biochem. Biotechnol., vol. 195, no. 1, pp. 51–67, Jan. 2023, doi: 10.1007/s12010-022-04090-5. DOI: https://doi.org/10.1007/s12010-022-04090-5
V. E. K. Vera and J. Silalahi, ‘Testing Of The Cream Formula Turmina Right Extract Against The Inhibition Of The Development Of Melanoma Cells,’ Int. J. Sci. Technol. Manag., vol. 3, no. 2, pp. 525–529, 2022. DOI: https://doi.org/10.46729/ijstm.v3i2.476
M. Urošević, L. Nikolić, I. Gajić, V. Nikolić, A. Dinić, and V. Miljković, ‘Curcumin: Biological activities and modern pharmaceutical forms,’ Antibiotics, vol. 11, no. 2, p. 135, 2022. DOI: https://doi.org/10.3390/antibiotics11020135
V. E. Kaban, N. Nasri, H. D. Syahputra, M. F. Lubis, and D. Satria, ‘Uji Aktivitas Antibakteri Ekstrak Daun Karenda (Carissa carandas Linn.) Terhadap Bakteri Propionibacterium acne dan Staphylococcus epidermidis’, J. Pharm. Health Res., vol. 4, no. 1, pp. 91–96, 2023. DOI: https://doi.org/10.47065/jharma.v4i1.3181
V. E. Kaban, N. Nasri, H. D. Syahputra, R. Fitri, Z. Rani, and M. F. Lubis, ‘Formulasi Sediaan Gel dari Ekstrak Metanol Biji Alpukat (Persea americana Mill.) Sebagai Penyembuh Luka Sayat Pada Tikus Jantan (Rattus norvegicus)’, Herb. Med. J., vol. 5, no. 2, pp. 48–54, 2022. DOI: https://doi.org/10.58996/hmj.v5i2.50
R. Fitri, H. D. Syahputra, N. Nasri, V. E. Kaban, and Z. Rani, ‘Formulation of a biocellulose mask containing the essence of Aloe vera (L.) Burm. f combination with vitamin E as anti-aging, Sci. Pharm. Sci., no. 6 (40), pp. 36–42, 2022. DOI: https://doi.org/10.15587/2519-4852.2022.268987
A. D. Otari, R. A. Patil, and C. D. Upasani, ‘Formulation And Evaluation Of Transdermal Herbal Gel Formulation Containing Ethanolic Extract Of Zingiber Officinale.’, J. Adv. Zool., vol. 45, 2024.
S. N. Palette, ‘Efek Ekstrak Daun Jarak Pagar (Jatropha curcas L.) terhadap penyembuhan luka pada mukosa rongga mulut (ulser traumatik) tikus putih jantan galur wistar (Rattus norvegicus)= effects of daun jarak pagar leaf extract (Jatropha curcas L.) on wound healing in oral mucosa (traumatic ulcer) of wistar male white rats (Rattus norvegicus)’, PhD Thesis, Universitas Hasanuddin, 2023. Accessed: Aug. 04, 2024. [Online]. Available: http://repository.unhas.ac.id/id/eprint/34331/
S. Prasad, S. C. Gupta, A. K. Tyagi, and B. B. Aggarwal, ‘Curcumin, a component of golden spice: from bedside to bench and back,’ Biotechnol. Adv., vol. 32, no. 6, pp. 1053–1064, 2014. DOI: https://doi.org/10.1016/j.biotechadv.2014.04.004
B. Chauhan, G. Kumar, N. Kalam, and S. H. Ansari, ‘Current concepts and prospects of herbal nutraceutical: A review,’ J. Adv. Pharm. Technol. Res., vol. 4, no. 1, pp. 4–8, 2013. DOI: https://doi.org/10.4103/2231-4040.107494
S. Lakshmi, S. Gugulothu, C. Mohanty, T. Venkatachalam, and C. Nayak, ‘Development and evaluation of anti-inflammatory gel containing zinger officinale.’, NeuroQuantology, vol. 20, no. 12, p. 1030, 2022.
Y. He, Y. Yue, X. Zheng, K. Zhang, S. Chen, and Z. Du, ‘Curcumin, inflammation, and chronic diseases: how are they linked?’, Molecules, vol. 20, no. 5, pp. 9183–9213, 2015. DOI: https://doi.org/10.3390/molecules20059183
H. R. Rajeshwari et al., ‘Local drug delivery systems in the management of periodontitis: A scientific review,’ J. Controlled Release, vol. 307, pp. 393–409, 2019. DOI: https://doi.org/10.1016/j.jconrel.2019.06.038
V. E. Kaban, N. Nasri, K. Gurning, H. D. Syahputra, and Z. Rani, ‘Formulasi Sediaan Lip Cream Ekstrak Daun Miana (Coleus scuatellariodes [L] Benth.) sebagai Pewarna Alami’, INSOLOGI J. Sains Dan Teknol., vol. 1, no. 4, pp. 393–400, 2022. DOI: https://doi.org/10.55123/insologi.v1i4.719
I. Alexander and I. I. Krasnyuk, ‘Dermatologic gels spreadability measuring methods comparative study,’ Int J Appl Pharm, pp. 164–168, 2022. DOI: https://doi.org/10.22159/ijap.2022v14i1.41267
L. Chiarentin, C. Cardoso, M. Miranda, and C. Vitorino, ‘Rheology of Complex Topical Formulations: An Analytical Quality by Design Approach to Method Optimization and Validation,’ Pharmaceutics, vol. 15, no. 7, p. 1810, 2023. DOI: https://doi.org/10.3390/pharmaceutics15071810
S. Nurman, R. Yulia, Irmayanti, E. Noor, and T. Candra Sunarti, ‘The optimization of gel preparations using the active compounds of arabica coffee ground nanoparticles,’ Sci. Pharm., vol. 87, no. 4, p. 32, 2019. DOI: https://doi.org/10.3390/scipharm87040032
P. G. Karamkar, A. Agrawal, and V. K. Chatap, ‘A review article: Formulation of topical gel by QbD approach,’ Adv Pharmacol Pharm, vol. 11, pp. 90–101, 2023. DOI: https://doi.org/10.13189/app.2023.110202
V. Y. Londhe and S. Sharma, ‘Formulation, characterization, optimization and in-vivo evaluation of methazolamide liposomal in-situ gel for treating glaucoma,’ J. Drug Deliv. Sci. Technol., vol. 67, p. 102951, 2022. DOI: https://doi.org/10.1016/j.jddst.2021.102951
P. Zhao et al., ‘Versatile Hydrogel Dressing with Skin Adaptiveness and Mild Photothermal Antibacterial Activity for Methicillin‐Resistant Staphylococcus Aureus‐Infected Dynamic Wound Healing,’ Adv. Sci., vol. 10, no. 11, p. 2206585, 2023. DOI: https://doi.org/10.1002/advs.202206585
Z. Rani, H. M. Nasution, V. E. Kaban, N. Nasri, and N. B. Karo, ‘Antibacterial activity of freshwater lobster (Cherax quadricarinatus) shell chitosan gel preparation against Escherichia coli and Staphylococcus aureus,’ J. Appl. Pharm. Sci., vol. 13, no. 2, pp. 146–153, 2023. DOI: https://doi.org/10.7324/JAPS.2023.130216
M. Lukić, I. Pantelić, and S. D. Savić, ‘Towards optimal ph of the skin and topical formulations: From the current state of the art to tailored products,’ Cosmetics, vol. 8, no. 3, p. 69, 2021. DOI: https://doi.org/10.3390/cosmetics8030069
Y. Jing et al., ‘Preparation, characterization and drug release properties of pH sensitive Zingiber officinale polysaccharide hydrogel beads’, Int. J. Biol. Macromol., p. 130376, 2024. DOI: https://doi.org/10.1016/j.ijbiomac.2024.130376
A. Kandwal, R. K. Mamgain, and P. Mamgain, ‘Comparative evaluation of turmeric gel with 2% chlorhexidine gluconate gel for treatment of plaque induced gingivitis: A randomized controlled clinical trial’, AYU Int. Q. J. Res. Ayurveda, vol. 36, no. 2, pp. 145–150, 2015. DOI: https://doi.org/10.4103/0974-8520.175537
W. Y. Tong et al., ‘Antimicrobial wound dressing film utilizing cellulose nanocrystal as drug delivery system for curcumin,’ Cellulose, vol. 25, pp. 631–638, 2018. DOI: https://doi.org/10.1007/s10570-017-1562-9
D. Satria, U. Harahap, A. Dalimunthe, A. W. Septama, T. Hertiani, and N. Nasri, ‘Synergistic Antibacterial Effect of Ethyl Acetate Fraction of Vernonia amygdalina Delile Leaves with Tetracycline against Clinical Isolate Methicillin-Resistant Staphylococcus aureus (MRSA) and Pseudomonas aeruginosa’, Adv. Pharmacol. Pharm. Sci., vol. 2023, pp. 1–11, Feb. 2023, doi: 10.1155/2023/2259534. DOI: https://doi.org/10.1155/2023/2259534
A. Dalimunthe, D. Pertiwi, M. Muhammad, and D. Satria, ‘Analysis of antioxidant activity, total phenolic and flavonoid contents of ethanol extract of Litsea cubeba Lour. Bark’, in E3S Web of Conferences, EDP Sciences, 2021, p. 08005. DOI: https://doi.org/10.1051/e3sconf/202133208005