Main Article Content

Mutia Dena
Gabena Indrayani Dalimunthe
Minda Sari Lubis
Yayuk Putri Rahayu

Abstract

Staphylococcus aureus is one of the bacteria that can cause infectious diseases of the skin, one of which is purulent wound disease. Aloe vera (Aloe vera L.) belongs to the family Asphodelaceae, which is a plant known to have antibacterial compounds. The study was conducted experimentally. The independent variable is the concentration of aloe vera leaf flesh, 20%, 30% and 40%. The concentration of aloe leaf meat ointment is 20%, 30%, and 40%. The dependent variables are the antibacterial activity of aloe vera leaf meat ointment preparations against staphylococcus aureus bacteria. The results of the antibacterial Zone of Inhibition (ZOI) value of aloe vera leaf meat against staphylococcus aureus of 8.1 mm (20% concentration); 8.5 mm (concentration 30%); and 10.5 mm (concentration 40%). Antibacterial ZOI value of aloe leaf meat ointment against staphylococcus aureus by 10.6 mm (F1 20%), 11.5 mm (F2 30%), and 12.5 mm (F3 40%). The test results on purulent wounds in rat test animals get moderate healing scores on F1, F2, F3 and K + (gentamicin sulfate 0.1%) strong, so it can be said that aloe vera leaf meat ointment preparations can inhibit antibacterial growth in purulent wound healing even with a moderate category compared to Gentamicin ointment 0.1% with a strong (sensitive) category.


 

Downloads

Download data is not yet available.

Article Details

How to Cite
Dena, M., Dalimunthe, G. I., Lubis, M. S., & Rahayu, Y. P. (2023). Formulation and antibacterial activity test of aloe vera leaf meat ointment preparation (Aloe vera L.) on the growth of bacteria Staphylococcus aureus on healing purulent wounds. Journal of Pharmaceutical and Sciences, 6(5-si), 268–275. https://doi.org/10.36490/journal-jps.com.v6i5-si.388
Section
Original Articles

References

Agustin, E., Prasetyo, D., & Muchtar, M. (2022). Formulasi dan uji aktivitas antibakteri sediaan spray antiseptik tangan infusa daun kemangi (ocimum basilicum l.) dan lidah buaya (aloe vera (l.) burm.f.) terhadap bakteri staphylococcus aureus atcc 25923. Jurnal Farmasi Higea, 14(2), 120. https://doi.org/10.52689/higea.v14i2.425

Alepandi, M., Wahyudi, J., & Tiranda, Y. (2022). Efektivitas pemberian aloevera pada proses penyembuhan luka bakar: literature review. JKM Jurnal Keperawatan Merdeka, 2(1), 15-29. https://doi.org/10.36086/jkm.v2i1.1154

Anief, M., (2005), Farmasetika, 29-30, Gadjah Mada University Press, Yogyakarta.

Apriani, A. and Fathir, A. (2021). Daya hambat getah lidah buaya (aloe vera) terhadap pertumbuhan bakteri staphylococcus aureus. Jurnal Sehat Indonesia (Jusindo), 3(2), 40-48. https://doi.org/10.36418/jsi.v3i2.28

Arif, T. and Solikhah, F. (2023). Perbedaan dua dosis ekstrak lidah buaya terhadap glukosa darah tikus diabetes mellitus. Jurnal Keperawatan, 21(1), 9-21. https://doi.org/10.35874/jkp.v21i1.1130

Beland, F. (2006). An evaluation of the biological and toxicological properties of aloe barbadensis(miller), aloe vera. Journal of Environmental Science and Health Part C, 24(1), 103-154. https://doi.org/10.1080/10590500600614303

Cappuccino, dan sarman J.G. (2013). Manual Laboratorium Mikrobiologi, Edisi 8. Jakarta: EGC. Hal. 290.

Depkes RI. (1995). Farmakope Indonesia. Jakarta: Departemen Kesehatan Republik Indonesia.

Depkes RI. (2000). Parameter Standar Umum Ekstrak Tumbuhan Obat. Jakarta: Dirjen POM, Direktorat Pengawasan Obat tradisional. Halaman 14-41.

Ditjen POM. (1995). Farmakope Indonesia, Edisi IV. Jakarta: Departemen Kesehatan Republik Indonesia.

Fani, M. and Kohanteb, J. (2012). Inhibitory activity of aloe vera gel on some clinically isolated cariogenic and periodontopathic bacteria. Journal of Oral Science, 54(1), 15-21. https://doi.org/10.2334/josnusd.54.15

Forno-Bell, N., Bucarey, S., García, D., Iragüen, D., Chacón, Ó., & Martín, B. (2019). Antimicrobial effects caused by aloe barbadensis miller on bacteria associated with mastitis in dairy cattle. Natural Product Communications, 14(12), 1934578X1989667. https://doi.org/10.1177/1934578x19896670

Furnawanthi, I., (2007), Khasiat dan Manfaat Lidah Buaya Si Tanaman Ajaib Ed.8, Jakarta Selatan: PT. AgroMedia Pustaka, Hal. 1-29.

Gunarti, N., Yuniarsih, N., S, R., Khoerunnisa, R., Allahuddin, A., Anggraeni, F., … & Ruhdiana, T. (2022). Artikel review: kandungan senyawa aktif tanaman untuk kesehatan kulit. Jfionline | Print Issn 1412-1107 | E-Issn 2355-696x, 14(2), 190-195. https://doi.org/10.35617/jfionline.v14i2.86

Harborne, J., (1996). Metode Fitokimia: Penuntun Cara Modern Menganalisis Tumbuhan. Cetakan kedua. Penerjemah: Padmawinata, K. dan I. Soediro. Bandung: Penerbit ITB.

Indriaty, S., Indrawati, T., & Taurhesia, S. (2016). Uji aktivitas kombinasi ekstrak air lidah buaya ( aloe vera l.) dan akar manis (glycyrrhiza glabra l.) sebagai penyubur rambut. Pharmaciana, 6(1). https://doi.org/10.12928/pharmaciana.v6i1.3235

Jawetz, E., Melnick, J. L., dan Adelburg, E. A. (2001). Mikrobiologi Kedokteran, Edisi XXII, Diterjemahkan oleh Bagian Mikrobiologi Fakultas Kedokteran Universitas Airlangga. Jakarta: Penerbit Salemba Medika. Hal.205-209.

Kuncari, Emma Sri., Iskandarsyah, dan Praptiwi. (2014). Evaluasi, Uji Stabilitas Fisik dan Sineresis Sediaan Gel yang Mengandung Minoksidil, Apigenin dan Perasan Herba Seledri (Apium graveolens L.) Depok : Fakultas Farmasi Universitas Indonesia.

Lawrence, R., Tripathi, P., & Jeyakumar, E. (2009). Isolation, purification and evaluation of antibacterial agents from aloe vera. Brazilian Journal of Microbiology, 40(4), 906-915. https://doi.org/10.1590/s1517-83822009000400023

Maulidina, F. and Kusumastuti, A. (2014). Pengaruh pemberian vitamin c terhadap kadar trigliserida lanjut usia setelah pemberian jus lidah buaya ( aloe barbadensis miller). Journal of Nutrition College, 3(4), 665-672. https://doi.org/10.14710/jnc.v3i4.6866

Parnomo, T. and Pohan, D. (2021). Test the effectiveness of aloe vera extract on the growth of escherichia coli in vitro. International Journal of Health Sciences and Research, 11(8), 211-224. https://doi.org/10.52403/ijhsr.20210831

Pelczar, M.J. dan E.C.S. Chan. (2005). Dasar- Dasar Mikrobiologi. Ed. II. Universitas Indonesia (UI-Press). Jakarta.

Pertiwi, P. and Rahayuningsih, H. (2012). Pengaruh pemberian jus lidah buaya terhadap kadar glukosa darah puasa pada wanita prediabetes. Journal of Nutrition College, 1(1), 107-114. https://doi.org/10.14710/jnc.v1i1.408

Prasetya, H. (2012). Prospek Cerah Beternak Sapi Perah (Pembibitan, Pemeliharaan, Manajemen Kesehatan dan Pengolahan Susu). Yogyakarta: Pustaka Baru Press.

Qahar, H. (2020). Pengaruh lidah buaya menurunkan kadar glukosa darah pada diabetes melitus tipe 2. Jurnal Ilmiah Kesehatan Sandi Husada, 12(2), 798-805. https://doi.org/10.35816/jiskh.v12i2.408

Rahmawati, U., Rahayu, M., Martsiningsih, M., Pudyastuti, R., Siregar, S., & Gustina, M. (2023). Pendampingan pembuatan hand sanitizer alami pada masyarakat mejing lor ambarketawang gamping sleman. Ejoin Jurnal Pengabdian Masyarakat, 1(3), 170-175. https://doi.org/10.55681/ejoin.v1i3.653

Salisu, B. and S, M. (2019). Phytochemical screening and antimicrobial activity of aqueous stem extract of aloe vera on some common pathogenic bacteria. Umyu Journal of Microbiology Research (Ujmr), 4(2), 49-56. https://doi.org/10.47430/ujmr.1942.009

Sánchez, M., González-Burgos, E., Iglesias, I., & Gómez-Serranillos, M. (2020). Pharmacological update properties of aloe vera and its major active constituents. Molecules, 25(6), 1324. https://doi.org/10.3390/molecules25061324

Tanaka, M., Misawa, E., Ito, Y., Habara, N., Nomaguchi, K., Yamada, M., … & Higuchi, R. (2006). Identification of five phytosterols from aloe vera gel as anti-diabetic compounds. Biological and Pharmaceutical Bulletin, 29(7), 1418-1422. https://doi.org/10.1248/bpb.29.1418.

Voight, R. (1994). Buku Pelajaran Teknologi Farmasi (Edisi 5), Terjemahan S. Noerono, Yogyakarta: Gajah Mada University Pres.

Most read articles by the same author(s)

1 2 3 > >>