Test The Effectiveness Of Catharanthus roseus Leaf Extract On Healing Incision Wounds On Rabbit Back Skin
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Abstract
Incision wounds are a form of damage to body tissues caused by sharp objects and can cause bleeding by involving the role of hemostasis so that inflammation occurs. One medicinal plant with potential as traditional medicine is the Catharanthus roseus leaf because it contains secondary metabolites such as alkaloids, flavonoids, saponins, and steroids. This study aims to determine the effect of Catharanthus roseus leaf extract on the healing of wounds on the skin of rabbit backs. Observations were made for 14 days on three rabbits whose backs were divided into six areas. In certain areas, it is wound along 2 cm with a depth of 0.1 cm to apply Catharanthus roseus leaf test ointment 2 times a day. Rabbits were tested with five treatment groups: extract concentration of 25%, 50%, and 75%, and positive control and negative control. The results showed that the wounds were positive by days 8 and 9, 75% concentration had closed, and all rabbits had no scabs. The results of statistical tests showed that blood tread leaf extract affected the parameter of wound length in rabbits, which was 0.000 (p˂0.05). It can be concluded that the preparation of Catharanthus roseus leaf extract with concentrations of 25%, 50%, and 75% provides the effectiveness of healing incision wounds in rabbits.
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References
Amfotis, M. L., Made, N., Suarni, R., & Arpiwi, N. L. (2022). Journal of Biological Sciences. 9(1), 139–151. https://doi.org/10.24843/metamorfosa.2021.v09.i01.p13
Anggraeni, L., & Bratadiredja, marline abdassah. (2018). Tanaman Obat Yang Memilki Aktivitas Terhadap Luka Bakar. Farmaka, 16(2), 51–59.
Dewi Ida Ayu, L.P., I Made, D., I. K., & A.D. (2013). Bioaktivitas Ekstrak Daun Tapak Dara (Catharanthus Roseus) Terhadap Periode Epitelisasi Dalam Proses Penyembuhan Luka Pada Tikus Wistar [Universitas Udayana]. https://onesearch.id/Record/IOS2118.article-4521
Francesco, F. De, Francesco, M. De, & Riccio, M. (2022). Hyaluronic Acid / Collagenase Ointment in the Treatment of Chronic Hard-to-Heal Wounds : An Observational and Retrospective Study.
Galomat, D. E., de Queljoe, E., Datu, O. S., & Studi Farmasi FMIPA UNSRAT Manado, P. (2021). Effect Of Giving Centella (Centella asiatica) (L) Urb) Leaves Extract Ointment On Wound Healing Of Male White Rats (Rattus norvegicus). Pharmacon, 10(4), 1205–1214.
Hakim, I. R., Lestari, F., & Priani, S. E. (2021). Kajian Pustaka Tanaman yang Berpotensi dalam Penyembuhan Luka Bakar. Prosding Farmasi, 7(1), 14–20. http://dx.doi.org/10.29313/.v7i1.25982
Hemmati, A. A., Larki-Harchegani, A., shabib, S., Jalali, A., Rezaei, A., & Housmand, G. (2018). Wound healing property of milk in full thickness wound model of rabbit. International Journal of Surgery, 54(April), 133–140. https://doi.org/10.1016/j.ijsu.2018.04.030
Ergina, Siti Nuryanti dan Indarini Dwi Pursitasari. (2014). Uji Kualitatif Senyawa Metabolit Sekunder Pada Daun Palado (Agave angustifolia) Yang Diekstraksi Dengan Pelarut Air Dan Etanol. J. Akad. Kim, 3(3), 165–172.
Mahmudah. (2013). Uji Aktivitas Film Kitosan yang Mengandung Asiatikosida sebagai Penutup Luka Bakar pada Tikus Putih Betina (Rattus Norvegicus) Galur Sprague Dawley Secara In Vivo. Jakarta: Penerbit Buku Kedokteran EGC, 105–119.
Megawati, S. (2020). Formulasi Dan Uji Efektivitas Penyembuhan Luka Sayat Salep Ekstrak Metanol Bunga Ginje (Thevetia Peruviana) Terhadap Kelinci Jantan New Zealand White. Jurnal Farmasi Udayana, 180. https://doi.org/10.24843/jfu.2020.v09.i03.p06
Meikahani, R., dan Kriswanto, S. (2015). Pengembangan Buku Saku Pengenalan Pertolongan Untuk Siswa Sekolah Menengah Pertama.
Mensie, M. Lovianie., S. N., & Mustika. (2018). Pengaruh Pemberian Sediaan Emulgel Kitosan-Ekstrak Daun Tapak Dara (Catharantus Roseus (L.) G. DON.) Dan Emulgel Kitosan-Ekstrak Kulit Pisang Ambon (Musa Paradisiaca L.) Untuk Penyembuhan Luka Bakar Pada Kelinci. Artikel Ilmiah Nurul, 3(2), 40–46.
Nasution, A. P. P. dan M. P. (2022). Skrining Fitokimia Dan Uji Sitotoksisitas Ekstrak Etanol Daun Tapak Dara (Catharanthus Roseus L.) Dengan Metode Brine Shrimp Lethality Test (Bslt). 1(April), 203–219.
Palumpun, E. F., Wiraguna, A. A. G. P., Medicine, P. P. A., Penyakit, D., Andrologi, D., Kedokteran, F., & Udayana, U. (2017). Pemberian ekstrak daun sirih ( Piper betle ) secara topikal meningkatkan ketebalan epidermis , jumlah fibroblas , dan jumlah kolagen dalam proses penyembuhan luka pada tikus jantan galur Wistar ( Rattus norvegicus ) Wimpie Pangkahila Menjadi tua adalah su. 5.
Rima Yulia Senja, Nur Rahmi Hidayati, dan Setyaningsih, I. (2014). Uji Efektivitas Salep Ekstrak Daun Mengkudu (Morinda citrifolia L.) Terhadap Luka Sayat Pada Kelinci Jantan (Oryctolagus cuniculus). Medical Sains : Jurnal Ilmiah Kefarmasian, 1(2), 100–107. https://doi.org/10.37874/ms.v1i2.21
Risa Rahma Putri, Rachmi Fanani Hakim, S. R. (2017). Pengaruh Ekstrak Daun Tapak Dara (Catharanthus Roseus) Terhadap Jumlah Fibroblas Pada Proses Penyembuhan Luka Di Mukosa Oral. Journal Caninus Denstistry, 2(1), 20–30.
Sembiring, E. E., & Nena Meo, M. L. (2020). Pengetahuan dan Sikap Berhubungan dengan Resiko Tertular Covid-19 pada Masyarakat Sulawesi Utara. NERS Jurnal Keperawatan, 16(2), 75. https://doi.org/10.25077/njk.16.2.75-82.2020
Sjamsuhidajat, R, W. de J. (1997). Buku Ajar Ilmu Bedah. EGC.
Wang, S., Tavakoli, S., Parvathaneni, R. P., Nawale, G. N., Oommen, O. P., Hilborn, J., & Varghese, O. P. (2022). Dynamic covalent crosslinked hyaluronic acid hydrogels and nanomaterials for biomedical applications. Biomaterials Science, 10(22), 6399–6412. https://doi.org/10.1039/d2bm01154a