Antidiabetic test of combination of insulin leaf extracts (Tithonia diversifolia (Hems.) A.Gray) and purple sweet tattoo (Ipomoea batatas (L.) Lam) leaf in male rats due to streptozotocin induction
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Abstract
Diabetes mellitus is a condition in which blood glucose levels are high enough in the body. Insulin leaves contain tannins, flavonoids and saponins which play a role in fighting free radicals. Purple sweet potato leaves have anthocyanin and phenolic flavonoid compounds that function as anti-diabetics. The combination of the two is expected to produce a synergistic effect to increase the effectiveness of antidiabetics. This research aims to find out the antidiabetic effect and the effective dose of a combination of insulin leaf extract and purple sweet potato leaves. This study used 25 rats as test animals which were divided into 5 groups. The blood glucose levels of the rats were measured using the GOD-PAP method. The results showed that the combination dose of insulin leaf ethanol extract 30 mg/kgBB and purple sweet potato leaves 120 mg/kgBB (combination group 2) had an anti-diabetic effect, an average of 97.49 and a positive control (glibenclamide) mean of 116.83 in reducing blood glucose levels in streptozotocin-induced mice. The research show that based on the One Way ANOVA analysis, the sig. ie 0.000 <0.05, then in conclusion the average of the five test groups has a significant difference.
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