Differences in Fermentation Media on Single Cell Protein (SCP) Production Using Saccharomyces cerevisiae
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Abstract
Utilization of pineapple peel waste and tofu liquid waste can reduce environmental impacts and is an alternative fermentation medium containing cheap and easily obtained carbohydrate sources and has the potential for the growth of Saccharomyces cerevisiae. Single Cell Protein (SCP) is a protein derived from microbes such as yeast. The purpose of this study was to determine the differences between pineapple peel waste and tofu liquid waste with the addition of different nutrients to SCP production using S. cervisiae. The research method was experimental. The independent variables were fermentation medium MK1 (pineapple peel waste; KH2PO4; sugar); MK2 (pineapple peel waste; KH2PO4; (NH4)2SO4; sugar), MT1 (tofu liquid waste; KH2PO4; sugar); and MT2 (tofu liquid waste; KH2PO4; (NH4)2SO4; sugar). The fermentation period was 0, 2, 4, and 6 days. The dependent variables were protein content, cell dry weight, glucose content, pH and temperature. The results of the SCP production research obtained the highest percentage increase in protein content in MT2 media (tofu liquid waste; KH2PO4; (NH4)2SO4; sugar) was 33.33% (day-2, increase in dry cell weight 84.90%), followed by MK2 media (pineapple peel waste; KH2PO4; (NH4)2SO4; sugar) was 13.15% (day-4, increase in dry cell weight 2.77%). The conclusion is that from both pineapple peel waste and tofu liquid waste substrates, the best PST production was obtained with the highest protein content in MT2 (tofu liquid waste media with the addition of KH2PO4; (NH4)2SO4; and sugar).
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References
Adi, P. K., Siti, C., dan Mashudi. (2019). Pengaruh Lama Waktu Fermentasi Limbah Buah Nanas (Ananas comosus L. Merr) Terhadap Kualitas Fisik Dan Kandungan Nutrien Menggunakan Aspergillus niger
Maryana, L., Ana, S., & Nugrahani, .A., (2016). Produksi Protein Sel Tunggal Dari Kultur Rhizopus oryzae Dengan Medium Limbah Cair Tahu. Palu. Universitas Tadulako. Hal 134 DOI: https://doi.org/10.22487/j24428744.2016.v2.i2.5987
Napitupulu, K. J., Rahayu, Y. P., Nasution, H. M., & Lubis, M. S. (2024). Produksi Protein Sel Tunggal dari Kultur Saccharomyces cerevisiae dengan Medium Limbah Kulit Nanas (Ananas comosus L. MERR). OBAT: Jurnal Riset Ilmu Farmasi Dan Kesehatan, 2 (2), 230–250. https://doi.org/10.61132/obat.v2i2.346
Harahap, M., Rahayu, Y. P., Lubis, M. S., & Yuniarti, R. (2024). Produksi Protein Sel Tunggal dari Kultur Saccharomyces cerevisiae dengan Medium Limbah Cair Tahu. Vitalitas Medis: Jurnal Kesehatan dan Kedokteran, 1(2), 109-128. DOI: https://doi.org/10.61132/obat.v2i2.346
Murari, C. S., da Silva, D. C. M. N., Schuina, G. L., Mosinahti, E. F., & Del Bianchi, V. L. (2019). Bioethanol production from dairy industrial coproducts. BioEnergy Research, 12, 112-122. DOI: https://doi.org/10.1007/s12155-018-9949-5
Ajayi, A. A., Lawal, B., Salubi, A. E., Onibokun, A. E., Oniha, M. I., & Ajayi, O. M. (2021, February). Pectinase production by Aspergillus niger using pineapple peel pectin and its application in coconut oil extraction. In IOP Conference Series: Earth and Environmental Science (Vol. 655, No. 1, p. 012014). IOP Publishing. DOI: https://doi.org/10.1088/1755-1315/655/1/012014
Somaye, F., Marizich., & Lale. (2008). SingleCell Protein (SCP) Production from Uf Cheese When by Kluyveromyces Marxianus. 18th National Congres on Food Technology. Iran. 16-18 oct
Lay, B. W. (1994). Analisis Mikroba Di Laboratorium. Jakarta: PT. Raja Grafindo Persada
Rahayu, Y. P. (2018). Uji Aktivitas Lipase dan Biosurfaktan dari Bakteri Keratinolitik
Rahayu, Y. P. dan Prasetyo, H. A. 2020. Uji Aktivitas Bakteri Keratinolitik Sebagai Penghasil Biosurfaktan (Assay of Keratinolytic Bacterial Activity to Producing Biosurfactant). Pharmauho: Jurnal Farmasi, Sains, dan Kesehatan2020;6(2):75-80 DOI: http://dx.doi.org/10.33772/pharmauho.v6i2.12533 DOI: https://doi.org/10.33772/pharmauho.v6i2.12533
Standar Nasional Indonesia (SNI), 1992. Cara Uji Makanan Minuman (SNI 01-2891-1992). Jakarta: BSN
Nasution, M. N., Feliatra., Irwan, E. (2021). “Analisis Pertumbuhan Protein Sel Tunggal (PST) dengan Media yang Berbeda”. Jurusan Ilmu Kelautan Fakultas Perikanan dan Kelautan, Universitas Riau. E-ISSN: 2721-8902. Vol. 26(1) DOI: https://doi.org/10.31258/jpk.26.1.47-53
Prastujati, A. U., Hilmi, M., Khusna, A., Sjofjan, O., Natsir, M. H., & Talitha, S. U. (2022, March). Optimization of Single Cell Protein (SCP) Production using Saccharomyces Cerevisiae by Response Surface Methodology (RSM) on Cell Biomass and Protein Percentage. In International Conference on Improving Tropical Animal Production for Food Security (ITAPS 2021) (pp. 483-489). Atlantis Press. DOI: https://doi.org/10.2991/absr.k.220309.093
Hezarjaribi, M., Ardestani, F., & Ghorbani, H. R. (2016). Single cell protein production by Saccharomyces cerevisiae using an optimized culture medium composition in a batch submerged bioprocess. Applied biochemistry and biotechnology, 179, 1336-1345. DOI: https://doi.org/10.1007/s12010-016-2069-9
Krisnan, R. (2005). The effect of application of tea waste (Camellia sinensis) fermented with Aspergillus niger on broiler. Jurnal Ilmu Ternak Dan Veteriner, 10(1), 1–5. https://doi.org/10.14334/JITV.V10I1.470
Kuswardani, I., & Widjajaseputra. (1998). Produksi Protein Sel Tunggal Phanerochaete chrysosporium Pada Media Limbah Cair Tahu yang Diperkaya : Kajian Optimasi Waktu Panen. Prosinding Seminar Nasional Teknologi Pangan dan Gizi. 604-613
Walker, L. J., Aldhous, M. C., Drummond, H. E., Smith, B. R. K., Nimmo, E. R., Arnott, I. D. R., & Satsangi, J. (2004). Anti-Saccharomyces cerevisiae antibodies (ASCA) in Crohn's disease are associated with disease severity but not NOD2/CARD15 mutations. Clinical & Experimental Immunology, 135(3), 490-496 DOI: https://doi.org/10.1111/j.1365-2249.2003.02392.x
Hakim, K., 2007, Pengaruh Penambahan Ammonium Sulfat Terhadap Produksi Etanol pada Fermentasi Umbi singkong (Manihot utilissima Pohl.) dengan Inokulum Saccharomyces cerevisiae, Skripsi, Fakultas Farmasi, Universitas Muhammadiyah Surakarta, Surakarta
Muljono,C.L., 1981, ”Growth of Microorganism in Biotechnology”, Verlag, Chemie, Weinheim
Masithoh, E. 2012. “Pengaruh Konsentrasi Sukrosa Terhadap Pertumbuhan Khamir Roti Saccharomyces cerevisiae pada Media Bekatul dalam Produksi Protein Sel Tunggal”. Jurusan Biologi, Universitas Sebelas Maret
Kurniawan, H. (2016). Kualitas nutrisi ampas kelapa (cocos nuficena) fermentasi menggunakan aspergillus niger. Buletin Peternakan, 40(1), 26–33. https://doi.org/10.21059/buletinpeternak.v40i1.9822 DOI: https://doi.org/10.21059/buletinpeternak.v40i1.9822
Poedjiadi,Anna dan F. M. Titin Supriyanti. (2006). Dasar-Dasar Biokimia. Jakarta. UI-Press
Muhammad et al, 2017. “Kajian Lama Fermentasi Terhadap Konsentrasi Glukosa Dan Alkohol Pada Pembuatan Tape Onggok”. Fakultas Teknologi Pertanian, Universitas Semarang. E-ISSN: 16939115. Vol.12 DOI: https://doi.org/10.26623/jtphp.v12i2.1812
Pawignya, H. 2011. Pembuatan Protein Sel Tunggal dari Limbah Nanas dengan Proses Fermentasi. Teknik Kimia Fakultas Teknologi Industri UPN “Veteran”. ISSN 1693-4393 Yogyakarta
Wignyanto, S., Suharjono, D., dan Novita, A. 2001. Pengaruh Konsentrasi Gula Reduksi Sari Hati Nanas Dan Inokulum Saccharomyces Cerevisiae Pada Fermentasi Etanol. Jurnal Teknologi Pertanian. 2(1): 68-77
Forsith, W.G.C V.C, Quesnel. 1963. Mechanisme of Cacoa Cunning Advence in Enzimologst. New York: Mc Graw Hill Book Co.
Cooney,C.L., 1981, ”Growth of Microorganism in Biotechnology”, Verlag, Chemie, Weinheim
Nigam, J.N. (1998). Single Cell Protein from Pineapple Cannery Effluent. World Journal of Microbiology and Biotechnology. 14, 693-696 DOI: https://doi.org/10.1023/A:1008853303596
Batubara, U. M., 2009. Pembuatan Pakan Ikan dari Protein Sel Tunggal Bakteri Fotosintetik Anoksigenik dengan Memanfaatkan Limbah Cair Tepung Tapioka yang Diuji pada Ikan Nila (Oreochormis niloticus). Skripsi. Departemen Biologi. USU: Medan
Marx, J.L. (1991). Revolusi Bioteknologi. Yayasan Obor Indonesia : Jakarta
Bahri, S., Aji, A., & Yani, F. (2019). Pembuatan bioetanol dari kulit pisang kepok dengan cara fermentasi menggunakan ragi roti. Jurnal Teknologi Kimia Unimal, 7(2), 85-100 DOI: https://doi.org/10.29103/jtku.v7i2.1252
Faridah, H. D., & Sari, S. K. (2019). Pemanfaatan mikroorganisme dalam pengembangan makanan halal berbasis bioteknologi. Journal of Halal Product and Research, 2(1), 33-43 DOI: https://doi.org/10.20473/jhpr.vol.2-issue.1.33-43
Algus, L. L. F. (2014). Isolasi khamir dari tetes tebu (Molase) dan potensinya dalam menghasilkan etanol (Doctoral dissertation, Universitas Islam Negeri Maulana Malik Ibrahim)
Umaiyah, A. S., Chairul, C., & Yenti, S. R. (2014). Fermentasi Nira Nipah Skala 50 Liter Menjadi Bioetanol Menggunakan Saccharomyces Cerevisiae (Doctoral dissertation, Riau University)
Azizah, N., Al-Barrii, A. N., & Mulyani, S. (2012). Pengaruh lama fermentasi terhadap kadar alkohol, pH, dan produksi gas pada proses fermentasio bioetanol dari whey dengan substitusi kulit nanas. Jurnal Aplikasi Teknologi Pangan, 1(3), 72–78
Idiawati, N., Harfinda, E. M., & Arianie, L. (2014). Produksi enzim selulase oleh aspergillus niger pada ampas sagu. Jurnal Natur Indonesia, 16(1), 1–9 DOI: https://doi.org/10.31258/jnat.16.1.1-9
Wina, E. (2005). Teknologi pemanfaatan mikroorganisme dalam pakan untuk meningkatkan produktivitas ternak ruminansia di indonesia. Wartazoa, 15(4), 173–186
Anggorodi, R. (2005). Ilmu Makanan Ternak Umum. Yogyakarta: Gadjah Mada University Press
Moran, J. (2005). Tropical Dairy Farming: Feeding Management for Small Holder Dairy Farmers in the Humid Tropics. Collingwood. Australia: Landlinks Press DOI: https://doi.org/10.1071/9780643093133