Test for Contamination of E. coli and Salmonella sp. Bacteria on Beef in a Traditional Market in Hamparan Perak Sub-district
Article Sidebar
Main Article Content
Page: 892-902
Abstract
Indonesian people prefer beef as a source of meat. More production and focus on high quality beef to meet the increasing demand for beef. The purpose of this study was to ascertain whether beef at the local market in Hamparan Perak District was contaminated with Salmonella sp. and E. coli. This research was conducted in January and February 2023 and is a descriptive study. Five samples of beef sold at the Klumpang Inpres Market, Hamparan Perak District, were used in this study. The findings revealed 18 bacterial isolates, most of which were round with intact edges, most of which were flat in height, and almost all of which were white. Seller 3 has the largest total number of microorganisms with a sample number of PS3 4.8 106 CFU / g, while seller 5 has the lowest number of PS5 samples 1.3 106 CFU / g. Contamination test results for Salmonella sp. and E.coli showed that all meat samples tested positive for Salmonella sp. and E. coli. A total of 4 isolates of gram-positive bacteria with purple color were found in the findings of bacterial staining, while there were 14 isolates of gram-negative bacteria with red and pink colors.
Downloads
Article Details
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
References
Acharya Tankeshwar. Salmonella shigella agar (SSA) composition, procedure and results in bacteriology laboratory diagnosis of bacterial disease. 2015
Ampou, E. E., Triyulianti, I., & Nugroho, S. C. (2015). Bakteri Asosiasi Pada Karang Scleractinia Kaitannya Dengan Fenomena La-Nina Di Pulau Bunaken. Jurnal Kelautan Nasional, 10(2), 55–64.
Apriani, Nur, dkk., 2014. Analisis Bakteri Patogen Enterik pada Produk Es Batu yang Dipasarkan di Kota Surabaya. Surabaya. Jurnal Ilmiah Biologi.
Arif, Sufyan. Masdiana, Ch. P. Aris, S., W. 2014. Uji Total Plate Count (Tpc) Dan Enterobacter Daging Total Plate Count (TPC) And Enterobacter of Goat Meat of Market in Malang. Univeristas Brawijaya.
Badan Standarisasi Nasional. 2009. Batas Maksimum Cemaran Mikroba dalam Pangan.
Dwidjoseputro, D. 1989. Dasar-Dasar Mikrobiologi. Jakarta: Djambatan
Fardiaz, S. 1993. Analisis Mirobiologi Pangan. Raja Grafindo Persada, Jakarta.
Hadioetomo, R. S. 1993. Mikrobiologi Dasar Dalam Praktek Teknik dan Prosedur Dasar Laboratorium. Gramedia, Jakarta.
Harijani, n., Rahadi, U. S., & Nazar, D. S. (2013). Isolasi Escherichia colipada Daging yang Diperoleh dari Beberapa Pasar Tradisional di Surabaya Selatan. Veterinaria Medika, 6(1), 39-44.
Jonsen, G.D. 2004. Prospek dan Preferensi Masyarakat Terhadap Konsumsi Daging Sapi Olohan Di Indonesia. S. FGW FOOD Conference. Jakarta 6-7 Oktober 2004.
Kasryno, F., W. Rosegrant, C. Ringler, S. Adiwibowo, R. Beresford, M. Bosworth, G.M. Collado, I. Gonarsya, A. Gulati, B. Isdijo, Natasukarya, D. Prabowo, E.G. Saiid, S.M.P. Tjonronegoro, dan P. Tjitropranoto. 2004. Strategi pembangunan pertanian dan pedesaan Indonesia yang memihak masyarakat miskin. Laporan ADBTA No. 3843-INO. Agricuture and Rural Development Strategy (ARDS) Study. AARD- CASER, ADB, SEAMEO-SEARCA in association with CRESCENT, Bogor.
Lay, W. B. 1994. Analisis Mikroba di Laboratorium. PT Raja Grafindo Persada, Jakarta.
Madigan, M.T., Martinko, J.M., Stahl, D.A., and Clark, D. 2011. Biology of Microorganisms Thirteenth Edition. PearsonEducation International. USA.
Maksum Radji., 2011, Buku Ajar Mikrobiologi: Panduan Mahasiswa Farmasi dan kedokteran, EGC, Jakarta. Hlm. 201-207.2
Miranti, D., Ismail, dan Razali. 2017. Jumlah cemaran bakteri Escherichia coli pada daging kambing di pasar tradisional Kota Banda Aceh. JIMVET. 01(4):631-636.
Narumi, H.E, Zuhriansyah dan Imam Mustofa. 2009. Deteksi Pencemaran Bakteri Salmonella sp. Pada Udang Putih (Panaeus merguiensis) Segar Di Pasar Tradisional Kotamadya Surabaya. Jurnal Ilmiah Perikanan dan Kelauatan, Volume 1,1:87-91. Fakultas Kedokteran Hewan Unair. Universitas Airlangga Surabaya.
Purwa Nunik, Junianto, H. T. (2012). Karakteristik Bakteri Caviar Nilem Dalam Perendaman Campuran Larutan Asam Asetat Dengan Larutan Garam Pada Penyimpanan Suhu Rendah (5-10 C). Jurnal Perikanan DanKelautan, 3(4), 171–175.
Putri, A. L., & Kusdiyantini, E. (2018). Isolasi dan identifikasi bakteri asam laktat dari pangan fermentasi berbasis ikan (Inasua) yang diperjualbelikan di Maluku-Indonesia. Jurnal Biologi Tropika, 1(2), 6–12.
Rahayu, S. A & Gumilar. M. H. 2017. Uji Cemaran Air Minum Masyarakat Sekitar Margahayu Raya Bandung Dengan Identifikasi Bakteri Escherichia coli. IJPST 4 (2) : 51.
Sudarsono A. 2008. Isolasi dan Karakterisasi Bakteri pada Ikan Laut dalam Spesies Ikan Gindara (Lepidocibium flavobronneum). Skripsi. Bogor: Institut Pertanian Bogor.
Sugioto, Kusuma A, dan Veronica W. 2015. Kandungan Mikroba Pada Daging Sapi Beberapa Pasar Tradisional Di Bandar Lampung. Jurnal Ilmiah Peternakan Terpadu Vol. 3(2): 27 -30
Susanto, E., Wenny, L. 2013. Analisis Kualitas Mikrobiologis Daging Sapi di Pasar Tradisional Kota Lamongan J. Ternak.Universitas Indonesia Press, Jakarta.
Syarifah, I., Novarieta, E. 2015. Deteksi Salmonella sp. pada Daging Sapi dan Ayam. Prosiding Seminar Nasional Teknologi Peternakan dan Veteriner.