Detection of Proteolytic Bacteria from Ileum of Gallus Gallus as a candidate probiotic agent of fermented poultry feeding
Article Sidebar
Main Article Content
Page: 1561-1567
Abstract
The ileum is part of the small intestine that functions as the absorption of food and for the growth and development of bacteria to degrade feed, such as lactic acid bacteria. The purpose of this study was to explore and detect proteolytic bacteria from the ileum of Gallus gallus as a candidate probiotic agent. The research method is a selection of proteolytic bacteria, catalase, and motility. The results showed that the average proteolytic index in isolates GP sp. 4, GP sp.3, and GP sp. 1 were 1.533, 1.531, and 1.500. Nine isolates were gram-positive and one gram-negative (GP sp. 3). The ten isolates could hydrolyze hydrogen peroxide, as indicated by the formation of air bubbles. The motility test showed that eight isolates were motile and the other two isolates were non-motile (GP sp.8 and GP sp.10). The ten proteolytic bacterial isolates obtained did not have the potential to be used as probiotic agents for fermented poultry feed because low proteolytic index value.
Downloads
Article Details
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
References
Ahmad, B., Nigar, S., Shah, S.S.A., Bashir, S., Ali, J., Yousaf, S., & Bangash, J, A, 2013. Isolation and Identification of Cellulose Degrading Bacteria from Municipal Waste and Their Screening for Potential Antimicrobial Activity. World Applied Scinces Journal, 1420-1426.
Asril, M & Leksikowati, S. S. 2019. Isolasi Dan Seleksi Bakteri Proteolitik Asal Limbah Cair Tahu Sebagai Dasar Penentuan Agen Pembuatan Biofertilizer. Elkawnie: Journal Of Islamic Science And Technology Vol. 5, No. 2
Beski, A.S., Swick, S.M & Iji, P.A. 2015. Specialized protein products in broiler chicken nutrition. Jurnal Animal Nutrition. 1: 47-53.
Djide, M.N & Wahyudin, E. 2008. Isolasi bakteri asam laktat dari ASI, dan potensinya dalam menurunkan kadar kolesterol secara in vitro. Farmasi dan Farmakologi, 12 (3):73-78.
Fardiaz, S. 1993. Analisis Mikrobiologi Pangan. PT. King of GrafindoPersada. Jakarta.
Hidayat, R. dan Alhadi, F. 2012. Identifikasi Streptococcus Equi dari Kuda yang diduga Menderita Strangles. Jurnal Ilmu Pertanian Indonesia, 17(3): 199-203.
Karina, A.N., Hussain, D.R., Johanes, E & Nawir, N.H., 2014. Isolasi dan Karakterisasi Bakteri Proteolitik dari Saluran Pembuangan Limbah Industri Tahu. Jurnal Biologi Universitas Hasanuddin. 1: 1 – 8.
Kumara, M., Kashyap, S.S.N., Vijay, R, Tiwari. R & Anuradha, M. 2012. Production and Optimization of Extra Cellular Protease from Bacillus sp.Isolated from Soil. International Journal of Advanced Biotechnology and Research 3(2):564–569.
Munifah, I. 2014. Isolasi, Selksi dan Identifikasi Bakteri Proteolitik serta Produksi Protease dari Terasi Cirebon. Prosiding Seminar nasional Inovasi Teknologi Pengolahan Produk dan Bioteknologi Kelautan dan PerikananV.
Nurmalinda A., Periadnadi & Nurmiati. 2013. Isolasi dan Karakterisasi Parsial Bakteri Indigenous Pemfermentasi dari Buah Durian (Durio zibethinus Murr.). Jurnal Biologi Universitas Andalas 2(1), 8-13.
Olajuyigbe FM, 2013. Optimized Production and Properties of Thermostable Alkaline Protease from Bacillus subtilisSHS-04 Grown on Groundnut (Arachis hypogaea) Meal. Advances in Enzyme Research 1(04):112
Rahmawati, N.H.F. 2016. Isolasi dan Karakterisasi Bakteri Proteolitik dari Feses Hewan Luwak. Jurnal Biologi Universitas Negeri Yogyakarta. 4: 1 – 8.
Rizaldi, R., Setyantini, W.H & Sudarno. 2018. Isolasi dan Karakterisasi Bakteri Proteolitik yang Berasosiasi dengan Lamun Enhalus acoroides di Pantai Bama, Taman Nasional Baluran, Situbondo, Jawa Timur. Jurnal Ilmiah Perikanan dan Kelautan. 1: 12 –20.
Saidah, A.N. 2014. Isolasi Bakteri Proteolitik Termofilik dari Sumber Air Panas Pacet Mojokerto dan Pengujian Aktivitas Enzim Protease. Jurnal Biologi. Pp: 1 – 10.
Strohl WA, Rouse H, Fisher BD. 2001. Microbiology. USA: Lippincott Williams & Wilkins.
Surono, IS 2004. Fermented Milk Probiotics and Health. Jakarta: Tri CiptaKarya.
Utami, J.L., Ekowati, C. N., Sumardi & Rosa, E. 2019. Prosiding Seminar Nasional Perhimpunan Biologi Indonesia XXV. Lampung
Yusmarini, Indrati, R., Utami, T & Marsono, Y. 2009.Isolasi dan Identifikasi Bakteri Asam Laktat Proteolitik dari Susu Kedelai yang Terfermentasi Spontan. Jurnal Natur Indonesia 12(1), 28-33.