Ekstraksi kombinasi ultrasonik dan enzimatis untuk senyawa asam klorogenat dari Cascara Kopi Arabika Kerinci
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Limbah Cascara Kopi Arabika (CKA) berpotensi tinggi sebagai sumber senyawa bioaktif seperti asam klorogenat yang memiliki aktivitas antioksidan. Pengoptimalan pelepasan senyawa aktif dapat dilakukan dengan menkombinasi metode ultrasonik dan enzimatis yang lebih efisien dan ramah lingkungan. Tujuan dari penelitian ini adalah untuk melihat efektivitas metode ekstraksi ultrasound-enzymatic-assisted extraction (UEAE) berpelarut akuades untuk senyawa asam klorogenat dari CKA menggunakan Response Surface Methodology-Box-Behnken Design (RSM-BBD). Tiga parameter ekstraksi seperti suhu sonikasi (35-45°C), konsenterasi enzim (10-20 mg/g), dan suhu enzimolisis (40-60°C) dirancang untuk memperoleh kadar asam klorogenat yang optimal. Berdasarkan data trend umum yang dihasilkan yaitu pada kondisi suhu sonikasi 40°C, konsenterasi enzim 10 mg/g, dan suhu enzimolisis 60°C dapat diperoleh kadar asam klorogenat tertinggi sebesar 42,55 mg/g ekstrak kering dan hasil verifikasi menunjukkan nilai sebesar 44,17 mg/g ekstrak kering dan dalam rentang prediksi model dengan tingkat kepercayaan 95%. Model RSM kuadratik dengan desain faktor saat ini belum mampu menjadi alat prediktif yang andal dan hanya mampu untuk mengeksplorasi rantang kondisi ekstraksi operasional.
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