Combined ultrasound and enzymatic-assisted extraction of chlorogenic acid compounds from Arabica Coffee Cascara Kerinci
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Abstract
Arabica Coffee Cascara (CKA) has high potential as a source of bioactive compounds such as chlorogenic acid, which has antioxidant activity. The release of active compound can be optimized by combining ultrasonic and enzymatic methods, which are more efficient and environmentally friendly. The aim of this study was to examine the effectiveness of ultrasound-enzymatic-assisted extraction (UEAE) using water as a solvent for chlorogenic acid compound from CKA using Response Surface Methodology Box-Behnken Design (RSM_BBD). Three extraction parameters, such as sonication temperature (35-45°C), enzyme concentration (10-20 mg/g), and enzymolysis temperature (40-60°C), were designed to obtain optimal chlorogenic acid content. Based on the general trend data, results indicated that the highest chlorogenic acid content was 42.55 mg/g of dry extract, and was achieved under specific conditions: a sonication temperature of 40°C, an enzyme concentration of 10 mg/g, and an enzymolysis temperature of 60°C. Verification tests showed a value of 44.17 mg/g dry extract within the model’s prediction range with a 95% confidence level. The current quadratic RSM model with the existing factor design is not yet a reliable predictive tool and only serves to explore the range of operational extraction conditions.
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References
Patay EB, Fritea L, Antonescu A, Antonescu A, Dobjanschi L. Coffea arabica: A Plant with Rich Content in Caffeine. The Question of Caffeine, InTech; 2017. https://doi.org/10.5772/intechopen.68149. DOI: https://doi.org/10.5772/intechopen.68149
Badan Pusat Statistik. Statistik Kopi Indonesia 2023. vol. 8. 2024.
Iriondo-DeHond A, del Pilar Salazar M, Domenech LG, Montoya JEZ, del Castillo MD. Coffee proteins: Functional food ingredients with molecular effects for sustainable health. In: Campos-vega R, Oomah BD, editors. Molecular Mechanisms of Functional Food, Hoboken, NJ: Wiley; 2023, p. 385. DOI: https://doi.org/10.1002/9781119804055.ch11
Remón J, Ravaglio-Pasquini F, Pedraza-Segura L, Arcelus-Arrillaga P, Suelves I, Pinilla JL. Caffeinating the biofuels market: Effect of the processing conditions during the production of biofuels and high-value chemicals by hydrothermal treatment of residual coffee pulp. J Clean Prod 2021;302:1–17. https://doi.org/10.1016/j.jclepro.2021.127008. DOI: https://doi.org/10.1016/j.jclepro.2021.127008
Serna-Jiménez JA, Siles JA, de los Ángeles Martín M, Chica AF. A Review on the Applications of Coffee Waste Derived from Primary Processing: Strategies for Revalorization. Processes 2022;10:1–26. https://doi.org/10.3390/pr10112436. DOI: https://doi.org/10.3390/pr10112436
Rodrigues da Silva M, Sanchez Bragagnolo F, Lajarim Carneiro R, de Oliveira Carvalho Pereira I, Aquino Ribeiro JA, Martins Rodrigues C, et al. Metabolite characterization of fifteen by-products of the coffee production chain: From farm to factory. Food Chem 2022;369:1–17. https://doi.org/10.1016/j.foodchem.2021.130753. DOI: https://doi.org/10.1016/j.foodchem.2021.130753
Singla M, Sit N. Application of ultrasound in combination with other technologies in food processing: A review. Ultrason Sonochem 2021;73:1–13. https://doi.org/10.1016/j.ultsonch.2021.105506. DOI: https://doi.org/10.1016/j.ultsonch.2021.105506
Serna-Jiménez JA, Torres-Valenzuela LS, Sanín Villarreal A, Roldan C, Martín MA, Siles JA, et al. Advanced extraction of caffeine and polyphenols from coffee pulp: Comparison of conventional and ultrasound-assisted methods. LWT 2023;177:1–10. https://doi.org/10.1016/j.lwt.2023.114571. DOI: https://doi.org/10.1016/j.lwt.2023.114571
Marathe SJ, Jadhav SB, Bankar SB, Kumari Dubey K, Singhal RS. Improvements in the extraction of bioactive compounds by enzymes. Curr Opin Food Sci 2019;25:62–72. https://doi.org/10.1016/j.cofs.2019.02.009. DOI: https://doi.org/10.1016/j.cofs.2019.02.009
Vo TP, Tran TQD, Phan TH, Huynh HD, Vo TTY, Vo NMK, et al. Ultrasonic-assisted and enzymatic-assisted extraction to recover tannins, flavonoids, and terpenoids from used tea leaves using natural deep eutectic solvents. Int J Food Sci Technol 2023;58:5855–64. https://doi.org/10.1111/ijfs.16688. DOI: https://doi.org/10.1111/ijfs.16688
Shishir MRI, Chen W. Trends of spray drying: A critical review on drying of fruit and vegetable juices. Trends Food Sci Technol 2017;65:49–67. https://doi.org/10.1016/j.tifs.2017.05.006. DOI: https://doi.org/10.1016/j.tifs.2017.05.006
Navarra G, Moschetti M, Guarrasi V, Mangione MR, Militello V, Leone M. Simultaneous determination of caffeine and chlorogenic acids in green coffee by UV/Vis spectroscopy. J Chem 2017;2017. https://doi.org/10.1155/2017/6435086. DOI: https://doi.org/10.1155/2017/6435086
Subadra OS, Deru MY. Skrining dan standarisasi serbuk simplisia berdasarkan perbandingan tingkat ketuaan daun nangka (Artocarpus heterophyllus Lamk.). Duta Pharma Journal 2024;4:2830–7054. https://doi.org/10.47701/djp.v4i2.4262. DOI: https://doi.org/10.47701/fwvnpe48
Kemenkes RI. Suplemen I Farmakope Herbal Indonesia. II. Jakarta: Kementerian Kesehatan Republik Indonesia; 2022.
Ariva AN, Widyasanti A, Nurjanah S. Pengaruh Suhu Pengeringan Terhadap Mutu Teh Cascara dari Kulit Kopi Arabika (Coffea arabica). Jurnal Teknologi Dan Industri Pertanian Indonesia 2020;12:21–8. https://doi.org/10.17969/jtipi.v12i1.15744. DOI: https://doi.org/10.17969/jtipi.v12i1.15744
Słowik-Borowiec M, Oklejewicz B, Wnuk M. A Cascara-Infused Caffeine Drink as a Social Beverage. Molecules 2025;30. https://doi.org/10.3390/molecules30132749. DOI: https://doi.org/10.3390/molecules30132749
Hu S, Gil-Ramírez A, Martín-Trueba M, Benítez V, Aguilera Y, Martín-Cabrejas MA. Valorization of coffee pulp as bioactive food ingredient by sustainable extraction methodologies. Curr Res Food Sci 2023;6. https://doi.org/10.1016/j.crfs.2023.100475. DOI: https://doi.org/10.1016/j.crfs.2023.100475
Li Z, Zhang C, Zhang Y, Zeng W, Igor C. Coffee cell walls-composition, influence on cup quality and opportunities for coffee improvements. Food Quality and Safety 2021;5:1–21. https://doi.org/10.1093/fqsafe/fyab012. DOI: https://doi.org/10.1093/fqsafe/fyab012
Özdemir M, Yıldırım R, Yurttaş R, Başargan D, Hakcı MB. A review of ultrasound-assisted extraction of bioactive compounds from coffee waste. Gıda The Journal of Food 2025;50:56–73. https://doi.org/10.15237/gida.gd24094. DOI: https://doi.org/10.15237/gida.GD24094
Lin B, Wang S, Zhou A, Hu Q, Huang G. Ultrasound-assisted enzyme extraction and properties of Shatian pomelo peel polysaccharide. Ultrason Sonochem 2023;98. https://doi.org/10.1016/j.ultsonch.2023.106507. DOI: https://doi.org/10.1016/j.ultsonch.2023.106507
Mutar ZH, Mohammed AA, Al-Baldawi IA. Optimization of Acetaminophen and Methylparaben Removal within Subsurface Batch Constructed Wetland Systems. Journal of Ecological Engineering 2022;23:228–39. https://doi.org/10.12911/22998993/143934. DOI: https://doi.org/10.12911/22998993/143934
Rosalinda S, Aulia HA, Widyasanti A, Mardawati E. Optimasi kondisi ekstraksi ultrasonikasi pada vitamin C buah delima (Punica granatum L.) menggunakan respon permukaan. Jurnal Ilmiah Rekayasa Pertanian Dan Biosistem 2021;9:143–58. https://doi.org/10.29303/jrpb.v9i2.266. DOI: https://doi.org/10.29303/jrpb.v9i2.266
Zhand S, Yang BS, Kang K, Jeong M. Robust pretreatment in combination with ultrasound-assisted surfactant to improve the enzymatic hydrolysis of Eucalyptus pellita. Bioresources 2024;19:2244–71. DOI: https://doi.org/10.15376/biores.19.2.2244-2271
Robinson PK. Enzymes: principles and biotechnological applications. Essays Biochem 2015;59:1–41. https://doi.org/10.1042/BSE0590001. DOI: https://doi.org/10.1042/bse0590001
Tong SC, Siow LF, Tang TK, Lee YY. Effect of Enzyme-Assisted Extraction on Structural and Functional Properties of Palm Kernel Protein. J Food Biochem 2025;2025. https://doi.org/10.1155/jfbc/8869943. DOI: https://doi.org/10.1155/jfbc/8869943
Ramadhanti A, Nurjanah S, Widyasanti A, Ainina N. Pemodelan kondisi hidrodistilasi minyak atsiri jahe merah (Zingiber officinale var. Roscoe) dengan menggunakan Response Surface Methodology. Agrointek : Jurnal Teknologi Industri Pertanian 2024;18:429–39. https://doi.org/10.21107/agrointek.v18i2.18904. DOI: https://doi.org/10.21107/agrointek.v18i2.18904
Munteanu IG, Apetrei C. Analytical methods used in determining antioxidant activity: A review. Int J Mol Sci 2021;22. https://doi.org/10.3390/ijms22073380. DOI: https://doi.org/10.3390/ijms22073380
Baliyan S, Mukherjee R, Priyadarshini A, Vibhuti A, Gupta A, Pandey RP, et al. Determination of Antioxidants by DPPH Radical Scavenging Activity and Quantitative Phytochemical Analysis of Ficus religiosa. Molecules 2022;27. https://doi.org/10.3390/molecules27041326. DOI: https://doi.org/10.3390/molecules27041326