Antioxidant activity test of ethanol extract of Helixanthera cylindrica (Jack) Danser mistletoe leaves on Moringa oleifera plants using the 2,2-diphenyl-1-picrylhydrazyl method
Main Article Content
Page: 648-657
Abstract
Free radicals can originate from within the human body as a result of metabolic processes from chemical reactions in the body, as well as from external sources or the environment, including emissions from motor vehicles, air pollution from factories, industrial chemicals, food and beverage substances, cigarette smoke, radiation, and sunlight. Antioxidants are components that can prevent cells or molecules from being oxidised by donating electrons or hydrogen atoms to free radicals. The impact of the reactivity of free radical compounds can result in cell or tissue damage, degenerative diseases, and even cancer. One of the parasites on the moringa plant, namely Helixanthera cylindrica (Jack) Danser, contains secondary metabolite compounds such as alkaloids, flavonoids, and saponins. This research aimed to determine whether the leaves of the Helixanthera cylindrica (Jack) Danser mistletoe have antioxidant activity using the 2,2-Diphenyl-1-Picrylhydrazyl (DPPH) method. The research method is a laboratory experiment with five concentrations and three repetitions. Subsequently, the antioxidant activity testing is conducted qualitatively using the DPPH method at a wavelength of 517 nm and utilising a Spectrophotometer UV-Vis. Based on the results of the antioxidant test, the extract of Helixanthera cylindrica (Jack) Danser leaves has extreme activity with an IC50 value of 28.74 ppm. In contrast, the comparison, vitamin C has an IC50 value of 1.57 ppm. The regression equation for the concentration of the mistletoe is y=0.3344x + 40.389 with an R2 value of 0.9726, and Vitamin C shows y=4.4065x + 43.079 with an R² value of 0.9677. It can be concluded that the leaves of Helixanthera cylindrica (Jack) Danser on the Moringa oleifera plant have extreme antioxidant activity.
Downloads
Article Details
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
References
Staniek, K., Gille, L. 2010. Is thymoquinone an antioxidant? e BMC Pharmacology 2010, 10(Suppl1): A9. https://bmcpharma.biomedcentral.com/articles/10.1186/1471-2210-10-S1-A9.
Kuntorini, E.M., dan Astuti, M.D. 2010. Penentuan Aktivitas Antioksidan Ekstrak Etanol Bulbus Bawang Dayak (Eleutherine AmericanaMerr). Jurnal Sains dan Terapan Kimia. 4(1):15-22.
Jawi, I.M., Suprapta, N.D., dan Sutirtayasa, I.W.P. 2007. Efek Antioksidan Ekstrak Umbi Ubijalar Ungu ( Ipomoiea batatas L.) terhadap Hati Setelah Aktivitas Fisik Maksimal dengan Melihat Kadar AST dan ALT Darah pada Mencit. Dexa Media, 20 (3) 65-71.
Badarinath, A., Rao, K., Chetty C.S., Ramkanth, S., Rajan, T., and Gnanaprakash, K. 2010. A Review on In-vitro Antioxidant Methods: Comparisons, Correlations, and Considerations. International Journal of PharmTech Research, 2010: 1276-1285.
Mandal, S., Yadav, S., Nema, R. 2009. A Review: Antioxidants. Journal of Chemical and Pharmaceutical Research. 2009. 102-104.
Erguder, B., Avci, A., Devrim, E., and Durak, I. 2007. Effects of cooking techniques on antioxidant enzyme activities of some fruits and vegetables. Turk. J. Med. Sci., 37(3): 151-156.
Winarsi, H. 2007. Antioksidan Alami dan Radikal Bebas. Cetakan ke-1. Penerbit Kanisius. Yogyakarta.
Wilar, F.K., Mongi, J., Kanter, J.W., Potalangi, N.O. 2021. Uji Efektivitas Antidiabetes Ekstrak Daun Benalu (Helixanthera cylindrica (Jack) Danser) di Tanaman Kelor pada Tikus Putih (Rattus norvegicus) Yang Diinduksi Aloksan. Skripsi. Fakultas MIPA UKI Tomohon.
Besin, C. S. M., Kanter, J. W., Maarisit, W., Tumbel, S. L., Pareta, D. N., & Montolalu, F. M. 2024. Studi Aktivitas Antioksidan Dari Ekstrak Etanol Daun Benalu (Dendrophthoe pentandra (L.) Miq) Pada Tanaman Pala. Biofarmasetikal Tropis (The Tropical Journal of Biopharmaceutical), 7(1), 27–32. https://doi.org/10.55724/jbiofartrop.v7i1.447.
Musfiroh, E., dan Syarif, S.H. 2012. Uji aktivitas peredaman radikal bebas nanopartikel emas dengan berbagai konsentrasi sebagai material antiaging dalam kosmetik. UNESA Journal of Chemistry. 1(2):18-25.
Nurjanah, Izzati, L., dan Abdullah, A. 2011. Aktivitas Antioksidan dan Komponen Bioaktif Kerang Pisau (Solen spp). ILMU KELAUTAN ISSN 0853-7291, September 2011. Vol. 16 (3) 119-124.
Rosane L.C., G.P. Zandoná, R.C. Rossi, C.D. Ferreira, J.F. Hoffmann. 2023. Solid-phase extraction for determination of phenolic compounds in food and beverage. Reference Module in Chemistry, Molecular Sciences and Chemical Engineering, Elsevier. ISBN 9780124095472, https://doi.org/10.1016/B978-0-443-15978-7.00001-1. (https://www.sciencedirect.com/science/article/pii/B9780443159787000011)
Yang, H., Q. Zhang, Y. Zeng, C. Cheng, T.E. Coldea, H. Zhao. 2024. Differences in structure, stability and antioxidant activity of melanoidins from lager and ale beers. Journal Lwt;205(April):116517. Available from: https://doi.org/10.1016/j.lwt.2024.116517.
Chen, T., Q. Zeng, M. Cao, L. Zhang, B. Adyari, C. Ma. 2024. In vitro antioxidant activity of Moringa oleifera Lam. leaf after Monascus purpureus fermentation and chemical component changes by untargeted metabolomics. Jo you're na f. Environ Res;118344. Available from: https://doi.org/10.1016/j.envres.2024.118344.
Wonggo, D., C. Anwar, V. Dotulong, A. Reo, N. Taher, R.A. Syahputra, et al. 2024. Subcritical water extraction of mangrove fruit extract (Sonneratia alba) and its antioxidant activity, network pharmacology, and molecular connectivity studies. J Agric Food Res. ;18(May):101334. Available from: https://doi.org/10.1016/j.jafr.2024.101334.
Ayed, A., L. Caputo, V. De Feo, H.S. Elshafie, F. Fratianni, F. Nazzaro, et al. 2024. Antimicrobial, anti-enzymatic and antioxidant activities of essential oils from some Tunisian Eucalyptus species. Heliyon.;10(14):e34518. Available from: https://doi.org/10.1016/j.heliyon.2024.e34518.
Rashid, Harun-Or.M., S. Akter, U. Habiba, F.R. Laboni, J. Uddin, Z.K. Labu, et al. 2023. Antioxidant, antibacterial, cytotoxic and thrombolytic activities of flowers of Mirabilis jalapa L: Possible role of phenolics and flavonoids. J Agric Food Res:100893. Available from: https://doi.org/10.1016/j.jafr.2023.100893.
Namuga, C., H. Muwonge, K. Nasifu, P. Sekandi, T. Sekulima, J.B. Kirabira. 2024. Hoslundia opposita vahl is a potential source of bioactive compounds with antioxidant and antibiofilm activity for wound healing. BMC Complement Med Ther.;24(1):1–16.
Ayad, R., S. Akkal. 2019. Chapter 12 - Phytochemistry and biological activities of Algerian Centaurea and related genera, Editor(s): Atta-ur-Rahman, Studies in Natural Products Chemistry, Elsevier, Volume 63. Pages 357-414. ISSN 1572-5995, ISBN 9780128179017. https://doi.org/10.1016/B978-0-12-817901-7.00012-5. (https://www.sciencedirect.com/science/article/pii/B9780128179017000125)
Ridho, E. A., 2013. Uji Aktivitas Antioksidan Ekstrak Metanol Buah Lakum (Cayratia trifolia) Dengan Metode DPPH (2,2-DIFENIL-1- PIKRILHIDRAZIL). Naskah Publikasi. Program Studi Farmasi, Fakultas Kedokteran Universitas Tanjungpura Pontianak.