Eksplorasi dan Potensi Antibakteri Isolat Bakteri dari Sedimen Perairan dan Limbah Cair Pabrik Kelapa Sawit (LCPKS) terhadap Bakteri Patogen

Abstrak

Antimicrobial resistance has become a major challenge, requiring exploration of new microbial sources for antibacterial compound discovery. This study aimed to explore, isolate, characterize, and evaluate the antibacterial potential of indigenous bacteria obtained from Batanghari River sediment, Lake Sipin sediment, mangrove sediment, and palm oil mill effluent (POME) against pathogenic bacteria. Bacterial isolates were obtained through isolation and purification methods, followed by morphological characterization, Gram staining, biochemical tests, and antibacterial activity evaluation using the disc diffusion method against Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, and Salmonella Typhi. The results showed diverse morphological and physiological characteristics among bacterial isolates from different environmental sources. Several isolates exhibited antibacterial activity with varying inhibition levels. Isolates SBHD 44 and SBHD 67 showed strong activity against S. aureus, while isolat LCM 7 from POME demonstrated the highest activity against E. coli with an inhibition zone of 21.07 mm. Several isolates from Lake Sipin and mangrove sediment also exhibited antibacterial activity against specific test bacteria. These findings indicate that aquatic sediments and POME are potential sources of indigenous bacteria producing antibacterial compounds for further investigation.