Formulasi Dan Aktivitas Antioksidan Sirup Air Kelapa Tua (Cocos nucifera L) Dan Ekstrak Buah Merah (Pandanus conoideus L) Dengan Metode DPPH
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Latar Belakang: Air kelapa tua (Cocos nucifera L.) berpotensi sebagai minuman kesehatan namun sering menjadi limbah. Buah merah (Pandanus conoideus L.) adalah tanaman endemik Papua yang kaya antioksidan. Kombinasi keduanya diharapkan dapat menghasilkan sediaan sirup herbal yang bermanfaat. Tujuan: Memformulasikan sirup kombinasi air kelapa tua dan ekstrak buah merah serta mengetahui aktivitas antioksidannya dengan metode DPPH. Metode: Ekstrak buah merah diperoleh dengan maserasi etanol 96%. Dilakukan skrining fitokimia pada kedua bahan. Sirup dibuat dengan tambahan gula, asam sitrat, vanila, dan CMC. Evaluasi fisik meliputi uji organoleptik, homogenitas, pH, viskositas, dan kejernihan. Aktivitas antioksidan diuji dengan metode DPPH pada λ 517 nm menggunakan spektrofotometer UV-Vis. Hasil: Skrining fitokimia menunjukkan air kelapa tua mengandung alkaloid; ekstrak buah merah mengandung flavonoid, tanin, saponin, dan alkaloid. Evaluasi sirup memenuhi syarat SNI: organoleptik (warna oranye kemerahan, bau khas, rasa manis), homogen, pH 5–6, viskositas 250 mPa·s, dan jernih. Nilai IC50 sirup pada tiga replikasi berturut-turut adalah 550,34 μg/mL; 2282,3 μg/mL; dan 2863,4 μg/mL, yang tergolong aktivitas antioksidan lemah (IC50 > 200 ppm). Kesimpulan: Sirup kombinasi air kelapa tua dan ekstrak buah merah memiliki aktivitas antioksidan lemah namun memenuhi standar mutu fisik sediaan. Sediaan ini berpotensi dikembangkan sebagai minuman kesehatan herbal.
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