Karakterisasi dan Uji Stabilitas Clay Mask Isolat Mangostin Sebagai Antijerawat
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Jerawat merupakan masalah kulit yang sering dialami oleh remaja dan orang dewasa muda, disebabkan oleh inflamasi folikel pilosebasea akibat proliferasi bakteri Propionibacterium acnes (P. Acnes). Pemanfaatan bahan kimia sintetis untuk pengobatan jerawat dapat memicu efek negatif, sehingga eksplorasi bahan alami sebagai agen antijerawat menjadi pilihan yang prospektif. Penelitian ini bertujuan untuk menentukan konsentrasi aktivitas antibakteri dari isolat mangostin terhadap P. acnes, memformulasikannya ke dalam sediaan clay mask, serta mengevaluasi efektivitas antibakteri sediaan yang dihasilkan. Uji Antibakteri ditentukan melalui metode difusi cakram dengan variasi kadar isolat mangostin (0,275%; 0,55% dan 1,1% b/v) . Kemudian, clay mask dibuat menggunakan campuran basis bentonit dan kaolin dalam empat formula berbeda. Karakterisasi sediaan mencakup evaluasi organoleptik, nilai pH, homogenitas, viskositas, waktu mengering , kemampuan penyebaran, daya lekat, serta pengujian stabilitas fisik melalui metode cycling test. Efektivitas antibakteri clay mask dievaluasi terhadap P. acnes dengan pendekatan difusi. Hasil penelitian menunjukkan bahwa isolat mangostin pada konsentrasi 0,275% menghasilkan diameter zona hambat sebesar 15,10 ± 0,64 mm yang termasuk kategori kuat. Seluruh formula clay mask memenuhi persyaratan evaluasi fisik dan tetap stabil selama pengujian cycling test. Setelah diformulasikan, aktivitas antibakteri masih dapat dipertahankan meskipun terjadi penurunan diameter zona hambat menjadi kategori sedang, dengan rentang 7,00–9,18 mm terhadap P. acnes. Berdasarkan hasil tersebut, isolat mangostin berpotensi dikembangkan sebagai bahan aktif alami dalam sediaan clay mask antijerawat karena tetap menunjukkan aktivitas antibakteri setelah proses formulasi.
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