Analisis Kandungan Merkuri dan Hidrokuinon dalam Krim Pemutih Wajah Daring Menggunakan Spektrofotometer Serapan Atom dan Kromatografi Cair Kinerja Tinggi
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Abstrak
Kulit merupakan organ terluar dari tubuh, dan berperan penting bagi tubuh. Saat ini kulit putih merupakan keinginan bagi setiap pria dan wanita. Merkuri dan hidrokuinon merupakan bahan – bahan yang memiliki mekanisme kerja untuk memutihkan kulit, namun memiliki efek berbahaya pada penggunanya. Penelitian ini dilakukan untuk mengetahui adanya merkuri dan hidrokuinon secara kualitatif dan kuantitatif, pada krim pemutih wajah yang beredar secara online di Kota Bandung. Analisis merkuri sampel didestruksi menggunakan destruksi gelombang mikro untuk menghilangkan zat – zat organik. Uji tetes warna merkuri dilakukan dengan menggunakan pereaksi KI 0,5 N, HCl 6 M, dan NaOH 2 N. Berdasarkan hasil uji kualitatif merkuri, didapatkan pada sampel dengan kode D dan N positif mengandung merkuri. Analisis kuantitatif merkuri dilakukan dengan menggunakan instrumen Spektrofotometer Serapan Atom (SSA) dengan panjang gelombang 253,7 nm. Analisis kualitatif hidrokuinon uji tetes warna dilakukan dengan menggunakan pereaksi FeCl3 5 %, fenantrolin, dan Benedict. Berdasarkan hasil uji kualitatif merkuri, didapatkan pada sampel dengan kode E dan H positif mengandung hidrokuinon. Analisis kuantitatif hidrokuinon dilakukan dengan menggunakan instrumen Kromatografi Cair Kinerja Tinggi (KCKT). Dari 17 Sampel didapatkan hasil bahwa 2 sampel mengandung merkuri dan 2 sampel mengandung hidrokuinon. Sampel dengan kode D dan N positif mengandung merkuri dengan kadar 2,776 ± 0,036 μg/g and 4,277 ± 0,021 μg/g, dan sampel dengan kode E dan H positif mengandung hidrokuinon dengan kadar 2,3745% dan 2,4798%.
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Referensi
Bonifant H, Holloway S. A review of the effects of ageing on skin integrity and wound healing. Br J Community Nurs 2019;24:S28–33. https://doi.org/10.12968/bjcn.2019.24.Sup3.S28. DOI: https://doi.org/10.12968/bjcn.2019.24.Sup3.S28
Rahmawati NW. Kepercayaan Konsumen Dengan Kepuasan Konsumen Klinik Kecantikan. Jurnal Imiah Psikologi 2021;9:472–81. https://doi.org/10.30872/psikoborneo. DOI: https://doi.org/10.30872/psikoborneo.v9i3.6456
Maranduca M, Branisteanu D, Serban D, Branisteanu D, Stoleriu G, Manolache N, et al. Synthesis and physiological implications of melanic pigments (Review). Oncol Lett 2019. https://doi.org/10.3892/ol.2019.10071. DOI: https://doi.org/10.3892/ol.2019.10071
Sahin SC. The potential of Arthrospira platensis extract as a tyrosinase inhibitor for pharmaceutical or cosmetic applications. South African Journal of Botany 2018;119:236–43. https://doi.org/10.1016/j.sajb.2018.09.004. DOI: https://doi.org/10.1016/j.sajb.2018.09.004
Wang G-H, Chen C-Y, Lin C-P, Huang C-L, Lin C, Cheng C-Y, et al. Tyrosinase inhibitory and antioxidant activities of three Bifidobacterium bifidum-fermented herb extracts. Ind Crops Prod 2016;89:376–82. https://doi.org/10.1016/j.indcrop.2016.05.037. DOI: https://doi.org/10.1016/j.indcrop.2016.05.037
Burger P, Landreau A, Azoulay S, Michel T, Fernandez X. Skin Whitening Cosmetics: Feedback and Challenges in the Development of Natural Skin Lighteners. Cosmetics 2016;3:36. https://doi.org/10.3390/cosmetics3040036. DOI: https://doi.org/10.3390/cosmetics3040036
Song Y, Chen S, Li L, Zeng Y, Hu X. The Hypopigmentation Mechanism of Tyrosinase Inhibitory Peptides Derived from Food Proteins: An Overview. Molecules 2022;27:2710. https://doi.org/10.3390/molecules27092710. DOI: https://doi.org/10.3390/molecules27092710
Soyata A, Chaerunisaa AY. Whitening Agent : Mekanisme, Sumber dari Alam dan Teknologi Formulasinya. Majalah Farmasetika 2021;6:169. https://doi.org/10.24198/mfarmasetika.v6i2.28139. DOI: https://doi.org/10.24198/mfarmasetika.v6i2.28139
BPOM. Laporan Tahunan Badan POM Tahun 2022. Jakarta: Badan Pengawas Obat dan Makanan Republik Indonesia; 2022.
Ramli FF. Clinical management of chronic mercury intoxication secondary to skin lightening products: A proposed algorithm. Bosn J Basic Med Sci 2020. https://doi.org/10.17305/bjbms.2020.4759. DOI: https://doi.org/10.17305/bjbms.2020.4759
Agorku ES, Kwaansa-Ansah EE, Voegborlo RB, Amegbletor P, Opoku F. Mercury and hydroquinone content of skin toning creams and cosmetic soaps, and the potential risks to the health of Ghanaian women. Springerplus 2016;5:319. https://doi.org/10.1186/s40064-016-1967-1. DOI: https://doi.org/10.1186/s40064-016-1967-1
Topping DC, Bernard LG, O’Donoghue JL, English JC. Hydroquinone: Acute and subchronic toxicity studies with emphasis on neurobehavioral and nephrotoxic effects. Food and Chemical Toxicology 2007;45:70–8. https://doi.org/10.1016/j.fct.2006.07.019. DOI: https://doi.org/10.1016/j.fct.2006.07.019
Juliano CCA. Spreading of Dangerous Skin-Lightening Products as a Result of Colourism: A Review. Applied Sciences 2022;12:3177. https://doi.org/10.3390/app12063177. DOI: https://doi.org/10.3390/app12063177
Park J-D, Zheng W. Human Exposure and Health Effects of Inorganic and Elemental Mercury. Journal of Preventive Medicine & Public Health 2012;45:344–52. https://doi.org/10.3961/jpmph.2012.45.6.344. DOI: https://doi.org/10.3961/jpmph.2012.45.6.344
Rohaya U, Ibrahim N, Jamaluddin J. Analisis Kandungan Merkuri (Hg) Pada Krim Pemutih Wajah Tidak Terdaftar Yang Beredar Di Pasar Inpres Kota Palu. Jurnal Farmasi Galenika (Galenika Journal of Pharmacy) (e-Journal) 2017;3:77–83. https://doi.org/10.22487/j24428744.2017.v3.i1.8143. DOI: https://doi.org/10.22487/j24428744.2017.v3.i1.8143
Rejeki DS, Pramiastuti O. Analisis Hidrokuinon Pada Lima Merk Produk Krim Malam Menggunakan Metode High Performance Liquid Chromatography (HPLC). Bhamada: Jurnal Ilmu Dan Teknologi Kesehatan (E-Journal) 2022;13:14–21. https://doi.org/10.36308/jik.v13i2.405. DOI: https://doi.org/10.36308/jik.v13i2.405
Guo C, Lv L, Liu Y, Ji M, Zang E, Liu Q, et al. Applied Analytical Methods for Detecting Heavy Metals in Medicinal Plants. Crit Rev Anal Chem 2023;53:339–59. https://doi.org/10.1080/10408347.2021.1953371. DOI: https://doi.org/10.1080/10408347.2021.1953371
Papadopoulos A, Assimomytis N, Varvaresou A. Sample Preparation of Cosmetic Products for the Determination of Heavy Metals. Cosmetics 2022;9:21. https://doi.org/10.3390/cosmetics9010021. DOI: https://doi.org/10.3390/cosmetics9010021
Paithankar H V. HPLC method validation for pharmaceuticals: A review. International Journal of Universal Pharmacy and Bio Sciences 2013;2:229–40.
Rohman A. Kimia Farmasi Analisis Untuk Mahasiswa. Yogyakarta: Pustaka Pelajar; 2022.
Harmita H. Petunjuk Pelaksanaan Validasi Metode Dan Cara Perhitungannya. Majalah Ilmu Kefarmasian 2004;1:117–35. https://doi.org/10.7454/psr.v1i3.3375. DOI: https://doi.org/10.7454/psr.v1i3.3375
Putri Efendy A, Malaha A, Abas AS. Identification of Mercury and Hydroquinone Content in Handbody Products Circulating in Gorontalo City. JHTS; 2023.
Rahman H, Wilantika I, Latief M. Analisis Kandungan Merkuri pada Krim Pemutih Ilegal di Kecamatan Pasar Kota Jambi menggunakan Spektrofotometri Serapan Atom (SSA). PHARMACY: Jurnal Farmasi Indonesia (Pharmaceutical Journal of Indonesia) 2019;16:59. https://doi.org/10.30595/pharmacy.v16i1.4350. DOI: https://doi.org/10.30595/pharmacy.v16i1.4350
Hadriyati A, Hartesi B, Fitri AA. Analisis Merkuri (Hg) pada Krim Pemutih yang Beredar di Klinik Kecantikan dalam Kecamatan Jelutung Kota Jambi. Cendekia Journal of Pharmacy 2020;4:102–9. https://doi.org/10.31596/cjp.v4i2.79. DOI: https://doi.org/10.31596/cjp.v4i2.79
Yulia R, Putri A, Hevira L. Analisis Merkuri Pada Merk Krim Pemutih Wajah dengan Metode Spektrofotometri Serapan Atom. Jurnal Katalisator 2019;4:103. https://doi.org/10.22216/jk.v4i2.4618. DOI: https://doi.org/10.22216/jk.v4i2.4618
Sarah KW. Analisis Hidrokuinon Dalam Sediaan Krim Malam “CW1” DAN “CW2” Dari Klinik Kecantikan “N” Dan “E” di Kabupaten Sidoarjo. vol. 3. 2014.
Taufiq Moh. Metode Destruksi Gelombang Mikro Menggunakan Variasi Konsentrasi Asam Nitrat Untuk Penentuan Logam Cd dan Pb Dalam Produk Makanan Bayi. Jurnal Kimia 2023:16. https://doi.org/10.24843/JCHEM.2023.v17.i01.p03. DOI: https://doi.org/10.24843/JCHEM.2023.v17.i01.p03
Svehla G., Suehla G. Vogel’s Qualitative Inorganic Analysis. Prentice Hall; 1996.
Ruchiyat Z, Jannah NR, Shalihat A. Qualitative and Quantitative Analysis of Hydroquinone Content in Marketplace Facial Whitening Creams. Chimica Didactica Acta 2023;11:21–5. https://doi.org/10.24815/jcd.v11i1.32162. DOI: https://doi.org/10.24815/jcd.v11i1.32162
Anugrah U, Delta, Rosdiana. Identifikasi Kandungan Hidrokuinon Pada Krim Pemutih Wajah Yang Beredar Di Pasar Andi Tadda Kota Palopo. Jurnal Kesehatan Luwu Raya 2022;1:17–22.
Jauria, Kostiawan Sukamto, Ariani Hutuba. Analysis of Hydroquinone Content in Whitening Cream Circulating in Gorontalo City Using Uv-Vis Spectrophotometry. Journal of Health, Technology and Science (JHTS) 2022;2:60–9. https://doi.org/10.47918/jhts.v2i4.283. DOI: https://doi.org/10.47918/jhts.v2i4.283
Suharyani I, Karlina N, Hidayati NR, Salsabila DZ, Annisa N, Sadira A, et al. Analisis Kualitatif Dan Kuantitatif Hidrokuinon Dalam Sediaan Kosmetika. Journal of Pharmacopolium 2022;4. https://doi.org/10.36465/jop.v4i3.807. DOI: https://doi.org/10.36465/jop.v4i3.807
Yang Z, Yan Y, Yu A, Pan B, Pignatello JJ. Revisiting the phenanthroline and ferrozine colorimetric methods for quantification of Fe(II) in Fenton reactions. Chemical Engineering Journal 2020;391:123592. https://doi.org/10.1016/j.cej.2019.123592. DOI: https://doi.org/10.1016/j.cej.2019.123592
Bose A. HPLC calibration process parameters in terms of system suitability test. Austin Chromatogr 2014;1:1–4.
Schober P, Boer C, Schwarte LA. Correlation Coefficients: Appropriate Use and Interpretation. Anesth Analg 2018;126:1763–8. https://doi.org/10.1213/ANE.0000000000002864. DOI: https://doi.org/10.1213/ANE.0000000000002864
Rubashvılı I, Karukhnıshvılı N, Lorıa K, Dvalı N. Validation of Swab Sampling and HPLC Methods for Determination of Meloxacam Residues on Pharmaceutical Manufacturing Equipment Surfaces for Cleaning Validation. Turk J Pharm Sci 2015;12:40–52. https://doi.org/10.5505/tjps.2015.43531. DOI: https://doi.org/10.5505/tjps.2015.43531
Taverniers I, Van Bockstaele E, De Loose M. Analytical Method Validation and Quality Assurance. Pharmaceutical Sciences Encyclopedia, Wiley; 2010, p. 1–48. https://doi.org/10.1002/9780470571224.pse396. DOI: https://doi.org/10.1002/9780470571224.pse396
Purnamasari ANR, Mubarak AS, Mulyono M. Analysis of Cadmium (Cd) Heavy Metal Using the Atomic Abrsoption Spectrophotometry (AAS) Method in Canned Crab Products at Balai Pengujian Mutu Hasil Perikanan (BPMHP) Semarang, Central Java. Journal of Marine and Coastal Science 2021;10:93. https://doi.org/10.20473/jmcs.v10i2.27661. DOI: https://doi.org/10.20473/jmcs.v10i2.27661
Cadungog DGE, Yee JRD, Sucgang RJ. Mercury in online skin-lightening cosmetics: A health risk assessment of products from selected Asian countries. Food and Chemical Toxicology 2025;204:115676. https://doi.org/10.1016/j.fct.2025.115676. DOI: https://doi.org/10.1016/j.fct.2025.115676
Nordlund J, Grimes P, Ortonne J. The safety of hydroquinone. Journal of the European Academy of Dermatology and Venereology 2006;20:781–7. https://doi.org/10.1111/j.1468-3083.2006.01670.x. DOI: https://doi.org/10.1111/j.1468-3083.2006.01670.x
Draelos ZD. Skin lightening preparations and the hydroquinone controversy. Dermatol Ther 2007;20:308–13. https://doi.org/10.1111/j.1529-8019.2007.00144.x. DOI: https://doi.org/10.1111/j.1529-8019.2007.00144.x