Effect of ethanol extract of nutmeg pulp (Myristica fragrans Houtt) on heart histopathology hyperglycemic wistar rats
Article Sidebar
Main Article Content
Page: 1469-1478
Abstract
The heart is an organ significantly impacted by diabetes mellitus problems, as elevated free radical generation and oxidative stress from hyperglycemia activate the apoptosis and necrosis pathways in cardiac myocytes. This study aimed to ascertain the histological characteristics of the cardiac tissue in rats subjected to hyperglycemia following the administration of ethanol extract from nutmeg fruit flesh (Myristica fragrans Houtt). This study employed rice as a diabetes inducer in 25 rats, categorized into 5 treatment groups: group 1 received Na-CMC, group 2 received metformin, group 3 received a dosage of 100 mg/KgBW, group 4 received a dosage of 200 mg/KgBW, and group 5 received a dosage of 300 mg/KgBW. The detected data included alterations in body weight, blood glucose levels, organ mass, and cardiac histology. The data were evaluated employing the One-Way ANOVA test at a 95% confidence level (p<0.05). The findings indicated that all groups treated with nutmeg fruit flesh ethanol extract exhibited a substantial enhancement in the histopathology of rat hearts compared to the negative control group 0,03 (p<0.05). The administration of nutmeg fruit flesh extract can ameliorate histopathological damage to the hearts of hyperglycemic rats at doses of 100 mg/KgBW, 200 mg/KgBW, and 300 mg/KgBW, indicating that nutmeg fruit flesh may serve as a cardioprotective drug in diabetic subjects.
Downloads
Article Details
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
References
Prehanawan Rp, Rayidah T, Mulyani As, Ariyanti R, Safitri An, Maharani S, Et Al. Waspadai Kolesterol Tinggi : Sebuah Artikel Pengabdian Kepada Masyarakat. Jurnal Pengabdian Masyarakat Medika 2022:12–7. Https://Doi.Org/10.23917/Jpmmedika.V2i1.457. DOI: https://doi.org/10.23917/jpmmedika.v2i1.457
Pintaningrum Y, Rahmat B, Ermawan R, Indrayana Y, Putra Aasmm. Penatalaksanaan Penyakit Kardiovaskular Dalam Praktek Sehari-Hari Untuk Dokter Umum. Vol. 3. 2021.
Rafey A, Amin A, Kamran M, Aziz Mi, Athar V, Niaz Si, Et Al. Evaluation Of Major Constituents Of Medicinally Important Plants For Anti-Inflammatory, Antidiabetic And Ages Inhibiting Properties: In Vitro And Simulatory Evidence. Molecules 2022;27:6715. Https://Doi.Org/10.3390/Molecules27196715. DOI: https://doi.org/10.3390/molecules27196715
International Diabetic Federation. Idf Diabetes Atlas Ninth Edition 2019. 9th Ed. 2019.
Murtiningsih Mk, Pandelaki K, Sedli Bp. Gaya Hidup Sebagai Faktor Risiko Diabetes Melitus Tipe 2. E-Clinic 2021;9:328. Https://Doi.Org/10.35790/Ecl.V9i2.32852. DOI: https://doi.org/10.35790/ecl.v9i2.32852
Adeyeoluwa Te, Balogun Fo, Ashafa Aot. In Vitro Comparative Assessment Of The Inhibitory Effects Of Single And Combined Spices Against Glucose-Synthesizing Enzymes. Tropical Journal Of Pharmaceutical Research 2020;19:1209–14. Https://Doi.Org/10.4314/Tjpr.V19i6.14. DOI: https://doi.org/10.4314/tjpr.v19i6.14
Devi K, Shalini K, Abhinaya R, Subha Ashley T. Study Of Antidiabetic And Antimicrobial Activity Of Various Medical Plants. Article In World Journal Of Pharmaceutical Research 2018;4:1–10. Https://Doi.Org/10.20959/Wjpr20186-11409.
Hasbullah, Faridah Dn, Dewi Fna, Indrasti D, Andarwulan N. Effect Of Nutmeg On Glycemic Status In Rat And Mice: A Systematic Review. Food Science And Technology (Brazil) 2023;43. Https://Doi.Org/10.1590/Fst.130122. DOI: https://doi.org/10.1590/fst.130122
Pashapoor A, Mashhadyrafie S, Mortazavi P. The Antioxidant Potential And Antihyperlipidemic Activity Of Myristica Fragrans Seed (Nutmeg) Extract In Diabetic Rats. Journal Of Human, Environment, And Health Promotion 2020;6:91–6. Https://Doi.Org/10.29252/Jhehp.6.2.7. DOI: https://doi.org/10.29252/jhehp.6.2.7
Al-Ishaq Rk, Abotaleb M, Kubatka P, Kajo K, Büsselberg D. Flavonoids And Their Anti-Diabetic Effects: Cellular Mechanisms And Effects To Improve Blood Sugar Levels. Biomolecules 2019;9:430. Https://Doi.Org/10.3390/Biom9090430. DOI: https://doi.org/10.3390/biom9090430
Athaillah A, Maulida Lubis R, Chandra P, Pangondian A, Rambe R. Uji Toksisitas Akut Ekstrak Rotan Sel (Daemonorop Melanochaetes Bl.) Terhadap Larva Udang (Artemia Salina Leach) Dengan Metode Brine Shrimp Lethality Test (Bslt). Journal Of Pharmaceutical And Sciences 2024:35–42. Https://Doi.Org/10.36490/Journal-Jps.Com.V7i1.463. DOI: https://doi.org/10.36490/journal-jps.com.v7i1.463
Maligan Jm, Pratiwi Dd, Widyaningsih Td. Studi Preferensi Konsumen Terhadap Nasi Putih Dan Nasi Jagung Putih Pada Pekerja Wanita Di Kantor Pemerintah Kota Malang. Indonesian Journal Of Human Nutrition 2019;6:41–52. Https://Doi.Org/10.21776/Ub.Ijhn.2019.006.01.5.
Rahayuningsih N, Pratama A, Suhendy H. Antidiabetica Activity Of Some Avocado Leaves Fractions (Persea Americanna Mill) In White Male With Alloxan Induction. Jurnal Kesehatan Bakti Tunas Husada : Jurnal Ilmu Ilmu Keperawatan, Analis Kesehatan Dan Farmasi 2020;20:43–51.
Yuliastri Wo, Lolok Nh, Ikawati N, Maghvira R. Uji Efek Ekstrak Bawang Hitam (Allium Sativum) Terhadap Penurunan Kadar Glikosa Darah Pada Tikus Putih (Rattus Novergicus L) Dengan Metode Tes Toleransi Glukosa Oral (Ttgo). Pharmacine : Journal Of Pharmacy, Medical And Health Science 2020;1:53–63. Https://Doi.Org/10.35706/Pc.V1i1.4423. DOI: https://doi.org/10.35706/pc.v1i1.4423
Ningsih Pw, Febriani H, Rasyidah. Pengaruh Ekstrak Bawang Batak (Allium Chinense G. Don.) Terhadap Ketebalan Aorta Tikus (Rattus Norvegicus) Diabetes Melitus. Biology Education Science And Technology 2021;4:1–6. DOI: https://doi.org/10.30743/best.v4i2.4102
Bpom. Badan Pengawas Obat Dan Makanan Nomor 18 Tahun 2021 Tentang Pedoman Uji Farmakodinamik Praklinik Obat Tradisional. Republik Indonesia: 2021.
Dwi Pratiwi P, Tiara N, Lestari I. Hepatoprotetor Activity Of Ethanol Extract Of Durian (Durio Zibethinus Murr.) Leaves On Paracetamol-Induced Mice. Journal Of Pharmaceutical And Sciences 2023;6:1170–7. DOI: https://doi.org/10.36490/journal-jps.com.v6i3.92
Putri Lm, Busman H, Ernawiati E. Pengamatan Kerusakan Histopatologi Jantung Pada Mencit Hiperglikemia Yang Diberi Ekstrak Daun Kersen (Muntingia Calabura). Bioeksperimen 2023;9:31–41.
Sholikah Ta, Wulandari S, Kusuma Trh, Muthmainah M. Efek Kardioprotektif Ekstrak Daun Kenikir (Cosmos Caudatus Kunth) Pada Tikus Putih (Rattus Novergicus) Model Diabetes Mellitus. Smart Medical Journal 2021;4:29. Https://Doi.Org/10.13057/Smj.V4i1.47952. DOI: https://doi.org/10.13057/smj.v4i1.47952
Rendayu I, Susianti, Putri Ad. Pengaruh Pemberian Teh Hijau (Camellia Sinensis) Terhadap Kerusakan Struktur Histopatologi Jantung Tikus Putih Jantan Galur Sprague Dawley Yang Dipapar Uap Rokok Elektronik. Jurnal Ilmu Kedokteran Dan Kesehatan, 2023;10:1777–81. DOI: https://doi.org/10.33024/jikk.v10i4.9566
Nur A, Fiskia E, Rahman I. Aktivitas Antiinflamasi Daging Buah Pala (Myristica Fragrans Houtt.) Pada Tikus Putih Yang Diinduksi Karageenan. Jfionline | Print Issn 1412-1107 | E-Issn 2355-696x 2022;14:10–6. Https://Doi.Org/10.35617/Jfionline.V14i1.87. DOI: https://doi.org/10.35617/jfionline.v14i1.87
Nur A, Rahman I. Uji Efek Analgetik Ekstrak Etanol Buah Pala Terhadap Tikus Putih Analgetic Effects Test Of Pala Fruit Ethanol Extract Against White Rats. Vol. 2. 2020. DOI: https://doi.org/10.33387/kmj.v2i2.2751
Soviana E, Pawestri C. Efek Konsumsi Bahan Makanan Yang Mengandung Beban Glikemik Terhadap Kadar Glukosa Darah. Darussalam Nutrition Journal 2020;4:94. Https://Doi.Org/10.21111/Dnj.V4i2.4047. DOI: https://doi.org/10.21111/dnj.v4i2.4047
Maligan Jm, Pratiwi Dd, Widyaningsih Td. Studi Preferensi Konsumen Terhadap Nasi Putih Dan Nasi Jagung Putih Pada Pekerja Wanita Di Kantor Pemerintah Kota Malang. Indonesian Journal Of Human Nutrition 2019;6:41–52. Https://Doi.Org/10.21776/Ub.Ijhn.2019.006.01.5. DOI: https://doi.org/10.21776/ub.ijhn.2019.006.01.5
Ojewale A, Mada S, Oyebadejo S, Afodun A, Aladeyelu O, Kolawole B. Cardioprotective Activities Of Ethanolic Extract Root Of Ageratum Conyzoides On Alloxan-Induced Cardiotoxicity In Diabetic Rats. Biomed Res Int 2020;2020. Https://Doi.Org/10.1155/2020/3189672. DOI: https://doi.org/10.1155/2020/3189672
Uthman Ademola Yusuf, Musonda Kambele, Wana Iputu, Mpeza Kalowa, Michelo Miyoba, Isabel Namfukwe Luambia Luambi, Et Al. Histological Investigation Of Aqueous Extract Of Cactus On The Heart Of Diabetic Wistar Rats. Gsc Biological And Pharmaceutical Sciences 2023;22:134–42. Https://Doi.Org/10.30574/Gscbps.2023.22.1.0020. DOI: https://doi.org/10.30574/gscbps.2023.22.1.0020
Alabi Td, Chegou Nn, Brooks Nl, Oguntibeju Oo. Effects Of Anchomanes Difformis On Inflammation, Apoptosis, And Organ Toxicity In Stz-Induced Diabetic Cardiomyopathy. Biomedicines 2020;8. Https://Doi.Org/10.3390/Biomedicines8020029. DOI: https://doi.org/10.3390/biomedicines8020029
Damayanti A, Kusuma Iy, Febrina D. Kombinasi Ekstrak Etanol Mesokarp Semangka (Citrullus Lanatus (Thunb.)) Dan Bawang Putih (Allium Sativum L.) Terhadap Kadar Glukosa Darah Dengan Metode God-Pap Pada Tikus Diabetesbination Of Ethanol Extract Of Watermelon Mesocarp (Citrullus Lanatus (Thunb.)) And Garlic (Allium Sativum L.) On Blood Glucose Level By God-Pap Method In Diabetic Rats. Pharmacy Genius 2023;2:60–73. Https://Doi.Org/10.56359/Pharmgen.V2i1.174. DOI: https://doi.org/10.56359/pharmgen.v2i1.174
Pasaribu Sf, Chandra P, Irwanto R, Anggraini Cd, Herviana H. Efek Antidiabetes Minuman Gemar (Germinated Black Rice) Pada Tikus Model Diabetes Melitus. Journal Of Pharmaceutical And Sciences 2023:323–30. Https://Doi.Org/10.36490/Journal-Jps.Com.V6i5-Si.315. DOI: https://doi.org/10.36490/journal-jps.com.v6i5-si.315
Sadeghi A, Mousavi Sm, Mokhtari T, Parohan M, Milajerdi A. Metformin Therapy Reduces Obesity Indices In Children And Adolescents: A Systematic Review And Meta-Analysis Of Randomized Clinical Trials. Childhood Obesity 2020;16:174–91. Https://Doi.Org/10.1089/Chi.2019.0040. DOI: https://doi.org/10.1089/chi.2019.0040
Tammuan M, Pakaya D, Salman M, Liwang Mni. Efek Metformin Terhadap Gambaran Histopatologi Otot Rangka Tikus Model Obesitas. Biomedika 2023:103–8. Https://Doi.Org/10.23917/Biomedika.V15i2.3303. DOI: https://doi.org/10.23917/biomedika.v15i2.3303
Paijo Arh, Indriawan Rt, Karisoh Mr, Susmantoyo Apm, Suryanto E, Runtuwene Mrj. Kemampuan Ekstrak Sekuensial Daging Buah Pala Sebagai Agen Hipoglikemik Untuk Penyerapan Glukosa. Chemistry Progress 2021;14:101. Https://Doi.Org/10.35799/Cp.14.2.2021.37114. DOI: https://doi.org/10.35799/cp.14.2.2021.37114
Kim K-S, Lee B-W. Beneficial Effect Of Anti-Diabetic Drugs For Nonalcoholic Fatty Liver Disease. Clin Mol Hepatol 2020;26:430–43. Https://Doi.Org/10.3350/Cmh.2020.0137. DOI: https://doi.org/10.3350/cmh.2020.0137
Sunarti S, Octavini P. Efek Antidiabetes Fraksi N-Heksana, Etil Asetat, Dan Air Dari Bunga Telang (Clitoria Ternatea L.) Pada Tikus Jantan Yang Diinduksi Streptozotocin-Nikotinamid. Journal Of Pharmaceutical And Sciences 2023;6:400–8. Https://Doi.Org/10.36490/Journal-Jps.Com.V6i2.96. DOI: https://doi.org/10.36490/journal-jps.com.v6i2.96
Magfirah, Utami Ik, Alaydrus S. Effect Of Seaweed Ethanol Extract On Cholesterol Levels And Obesity In White Rats. Jurnal Jamu Indonesia 2020;5:98–105. Https://Doi.Org/10.29244/Jji.V5i3.175. DOI: https://doi.org/10.29244/jji.v5i3.175
Hakimah Aa, Sjakoer Naa, Mubarakati Nj. Profil Histopatologi Otot Jantung Pada Tikus Hipertensi (Doca-Garam) Yang Dipapar Kombinasi Ekstrak Metanolik Benalu Teh Dan Benalu Mangga (Embtbm). Metamorfosa: Journal Of Biological Sciences 2021;8:359. Https://Doi.Org/10.24843/Metamorfosa.2021.V08.I02.P20. DOI: https://doi.org/10.24843/metamorfosa.2021.v08.i02.p20
Lohita Sari B, Lily Elfrieda Nsa, Marsuan K, Sapitri P, Hafidh A. Aktivitas Antioksidan Dan Studi In Silico Ekstrak Buah Pala (Myristica Fragrans Houtt). Jurnal Farmamedika (Pharmamedica Journal) 2022;7:28–40. Https://Doi.Org/10.47219/Ath.V7i1.142. DOI: https://doi.org/10.47219/ath.v7i1.142