The Effect of Akar Laka Stem Extract (Dalbergia parviflora Roxb.) on the Growth of Trichophyton rubrum
Article Sidebar
Main Article Content
Page: 2124-2132
Abstract
Akar laka (Dalbergia parviflora Roxb.) is a traditional medicinal plant used by communities in North Maluku to treat various types of wounds and skin conditions; however, scientific research on the antifungal activity of this plant’s stem is still limited. This study aims to identify the content of secondary metabolites and evaluate the antifungal activity of akar laka stem extract against the growth of Trichophyton rubrum in vitro. This is a laboratory experimental study conducted using the maceration method with 95% ethanol as a solvent to obtain a concentrated extract, followed by phytochemical screening to identify the classes of secondary metabolites. Antifungal activity testing was performed using the disk diffusion method with extract concentrations of 5%, 10%, and 15%, with ketoconazole used as the positive control and aqua pro injection as the negative control. The results showed that from 200 grams of crude drug, 43 grams of concentrated extract were obtained with a yield of 21.5%. Phytochemical screening results indicated the presence of alkaloids, saponins, and tannins, while flavonoids were not detected. In the antifungal activity test, the extract did not form an inhibition zone at any of the tested concentrations against T. rubrum, whereas the positive control exhibited a very strong inhibition zone of 26.7 mm. Based on the research results, it can be concluded that the extract of the akar laka stem (D. parviflora Roxb.) contains several secondary metabolites but did not exhibit antifungal activity against T. rubrum at the tested concentrations.
Downloads
Article Details
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
References
Khusnul, Hidana R, Kusmariani W. Uji Efektivitas Ekstrak Etanol Rimpang Lengkuas (Alpinia galanga L) Terhadap Pertumbuhan Trichophyton rubrum Secara in vitro. Vol. 17, Jurnal Kesehatan Bakti Tunas Husada. 2017. DOI: https://doi.org/10.36465/jkbth.v17i1.210
Wahyu FF, Putra CBPE, Sayoeti MFW. Resistensi Anti Jamur Pada Dermatofitosis: Review Literatur. J Ilmu Kedokt dan Kesehat. 2023;10(5):921–9. DOI: https://doi.org/10.33024/jikk.v11i5.13947
Azizah M, Akbar PTA, Hasanah M. Uji Aktivitas Anti Jamur Ekstrak Etanol Biji Alpukat (Persea americana Mill) Terhadap Jamur Kulit Tricophyton rubrum ATCC 28188, Epidermophyton floccosum ATCC 50266 dan Micospprum canis ATCC 32699). J Kesehat Saelmakers PERDANA. 2021;4(2):177–9.
Hayati AR, Singkam AR, Jumiarni D. Uji Antibakteri Ekstrak Etanol Daun Theobroma cacao L. Terhadap Pertumbuhan Escherichia coli Dengan Metode Difusi Cakram. BIOEDUSAINS J Pendidik Biol dan Sains. 2022;5(1):31–40. DOI: https://doi.org/10.31539/bioedusains.v5i1.3160
Fitriana K, Irwandi I, Muslihin AM. Formulasi dan Uji Karakteristik Stabilitas Fisik Sediaan Gel Sunscreen Ekstrak Etanol Buah Merah (Pandanus conoideus). J Etnofarmasi. 2023;1(01):30–7. DOI: https://doi.org/10.36232/jurnalfarmasiunimuda.v1i01.1720
Tumpu D, Muslihin AM, Hardia L. In Vitro Study of The Activity of Yellow Rope ( Anamirta cocculus ) Extract As An Antibacterial. J Penelit Pendidik IPA. 2025;11(4):382–7. DOI: https://doi.org/10.29303/jppipa.v11i4.10753
Irfan MA, Irwandi, Muslihin AM. Formulasi dan Efektivitas Sediaan Gel Pewarna Rambut Alami Ekstrak Buah Merah (Pandanus conoideus Lam.). J Etnofarmasi. 2025;03(01):26–32. DOI: https://doi.org/10.36232/jurnalfarmasiunimuda.v3i01.1960
Fahira IN, Muslihin AM, Irwandi. Uji Efektivitas Tanaman Patah Tulang (Euphorbia tirucalli) pada Mus musclus sebagai Antihiperglikemia. J Promot Prev. 2025;8(5):1183–94.
Aisyah H, Irwandi I, Budiyanto AB, Muslihin AM. Antibacterial Effectiveness Test of Wrap Leaf Extract (Smilax rotundifolia) Against Escherichia coli and Propionibacterium acnes Bacteria. J Penelit Pendidik IPA. 2025;11(4):527–32. DOI: https://doi.org/10.29303/jppipa.v11i4.10699
Khasanah N, Muslihin AM. Perbandingan Metode Ekstraksi Terhadap Kadar Total Flavonoid dan Alkaloid Daun Batik Papua (Graptophyllum pictum L. griff). J Etnofarmasi. 2025;3(01):10–25. DOI: https://doi.org/10.36232/jurnalfarmasiunimuda.v3i01.1964
Irwandi, Astuti RA, Rante H, Kursia S. Isolasi, Skrining Fitokimia dan Uji Aktivitas Antioksidan Fungi Endofit Tangkai Daun Murbei (Morus albaL.). J Etnofarmasi. 2022; DOI: https://doi.org/10.36232/jurnalfarmasiunimuda.v1i02.1731
Marzuki A, Djide MN, Sartika, T R. Uji Aktivitas Ekstrak Kulit Batang Banyuru (Pterospermum celebicum Miq.) dan Ekstrak Lengkuas (Alpinia galanga (L.)Willd.) Sebagai Antifungi Terhadap Trichophyton rubrum, Candida albicans dan Aspergillus niger. PHARMACON J Ilm Farm. 2018;7(3):354–62.
Fatmawati A, Rahmawati, Widyanti T, Padad T, Zilfianti. Potensi Antifungi Daun Kedondong Terhadap Isolat Candida albicans. J Ilmu Alam dan Lingkung [Internet]. 2024;15(2):1–6. Available from: https://journal.unhas.ac.id/index.php/jai2
Nurwahida WO, Muslihin AM, Hardia L. Antibacterial Activity Testing of Methanol Extract of Yellow Rope Barb ( Anamirta cocculus ). 2025;11(4):451–8. DOI: https://doi.org/10.29303/jppipa.v11i4.10760
Sari MN, Wiryosoendjojo K, Sulistyawati D. Aktivitas Antijamur Ekstrak Etanolik Daun Berenuk (Crescentia cujete, Linn.) terhadap Trichophyton rubrum. Setiabudi-Cihams 2020 [Internet]. 2020;1:1–9. Available from: https://202.56.165.211/index.php/proceeding/article/view/2
Asworo RY, Widwiastuti H. Pengaruh Ukuran Serbuk Simplisia dan Waktu Maserasi terhadap Aktivitas Antioksidan Ekstrak Kulit Sirsak. Indones J Pharm Educ. 2023;3(2):256–63. DOI: https://doi.org/10.37311/ijpe.v3i2.19906
Apriani P, Marcellia S, Nofita. Uji Aktivitas Antijamur Ekstrak Etanol Kulit Buah Mahoni (Swietenia mahagoni L .) Terhadap Candida albicans. Anal Environ Chem. 2023;8(1):1–10. DOI: https://doi.org/10.59638/junomefar.v2i1.702
Saepudin S, Dewi L, Nurmalasari R, Endah K, Taufik septiyan hidayat, Yunita al azzahra. Skrining Fitokimia dari Tiga Tanaman Famili Asteraceae dengan Berbagai Pereaksi Kimia. Parapemikir J Ilm Farm. 2024;13(3):333–47. DOI: https://doi.org/10.30591/pjif.v13i3.7069
Oktavia farida dwi, Sutoyo S. Skrining Fitokimia, Kandungan Flavonoid Total, Dan Aktivitas Antioksidan Ekstrak Etanol Tumbuhan Selaginella doederleinii. J Kim Ris. 2021;6(2):141–53. DOI: https://doi.org/10.20473/jkr.v6i2.30904
Hanifa NI, Wirasisya DG, Muliani AE, Utami SB. Jurnal Biologi Tropis Phytochemical Screening of Decoction and Ethanolic Extract of Amomum dealbatum Roxb . Leaves. J Biol Trop Orig. 2021;21(2):510–8. DOI: https://doi.org/10.29303/jbt.v21i2.2758
Septiani S, Getara VA, Ratnasari D. Analisis Antioksidan Pada Minuman Jahe Instan Menggunakan Metode 1,1-Diphenyl-2-Picrylhidrazyl (DPPH). J Pendidik dan Konseling. 2022;4(6):286–92.
Aristyawan AD, Yuliarni FF, Surahmaida, Suryandari M, Nony ari anggraini. Skrining Fitokimia Ekstrak Etanol Jamur Kuping Hitam (Auricularia nigricans) Dengan Metode Soxletasi Phytochemical Screening Of Ethanol Extract 96% Black Ear Mushroom (Auricularia nigricans) by soxletation method. Sitawa J Farm Sains dan Obat Tradis Vol. 2024;3(2):114–23.
Sulistyarini I, Sari diah arum, Wicaksono tony ardian. Skrining Fitokimia Senyawa Metabolit Sekunder Batang Buah Naga (Hylocereus polyrhizus) Indah. J Ilm Cendekia Eksakta. 2020;5(1):1–66.
Lestari YD, Permatasari S, Oktasari A. Antioxidant Activity Testing of Extract Kweni Peel (Mangifera odorata Griff). Indones J Chem Environ. 2020;3(2):11–20. DOI: https://doi.org/10.21831/ijce.v3i2.43508
Rezky khairun nisaa nur, Eka tyas wahyuni leny, Putri A. Skrining Fitokimia dan Studi Komparasi Kadar Flavonoid Dalam Bunga , Daun , dan Kulit Batang Pinus ( Pinus merkusii ). J Kesehat Jompa. 2025;4(1). DOI: https://doi.org/10.57218/jkj.Vol4.Iss1.1422
Saputri R, Hakim AR. Penetapan Kadar Flavonoid Total Dan Kadar Fenolik Total Ekstrak Kulit Buah Pitanak ( Nephelium sp .). J Pharm Care Sci. 2022;2(2):92–6. DOI: https://doi.org/10.33859/jpcs.v2i2.191
Jastria P, Muhammad almuizzu tilu, Risky J. Uji Aktivitas Antijamur Ekstrak Etanol Biji Keben ( Barringtonia asiatica L .) Terhadap Jamur Malassezia furfur Antifungal Activity of Ethanol Extract of Keben Seeds ( Barringtonia asiatica L .) Against Malassezia furfur Info Artikel : Penyakit infeksi me. J Pharm Mandala Waluya. 2023;2(4):199–210. DOI: https://doi.org/10.54883/jpmw.v2i4.23
Amelia R, Asih NM, Lati P, Sulastri L. Aktivitas Antifungi Ekstrak Nades Daun Pacar Kuku ( Lawsonia inermis L ) Dan Daun Alpukat ( Persea americana ) Terhadap Pityrosporum Ovale Antifungal Activities Of Nail Henna ( Lawsonia inermis L ) Leaves And Avocado ( Persea americana ) Leaves. J Syst STF Muhammadiyah Cirebon. 2022;7(1). DOI: https://doi.org/10.37874/ms.v7i1.295
Repita anis jungjunan, Pudji R, Yulyuswarni, Dias A. Antibacterial Activity And Effectiveness Test Of Bandotan (Ageratum conyzoides Linn.) Leaves Ethanol Extract Against Staphylococcus aureus UJI. J Anal Farm. 2023;8(1). DOI: https://doi.org/10.33024/jaf.v8i1.9269
Bella tasya kimberly, Chylen setiyo rini. Effectiveness Test of Okra Fruit (Abelmoschus esculentus) Extract on The Growth of Trichophyton rubrum. J Medica Lab Sci Technol. 2022;5(2):86–90. DOI: https://doi.org/10.21070/medicra.v5i2.1647
Wisudawan, Arsal ASF, Imron A, Bamahri A, Makmun A. Uji Efektivitas Daya Hambat Ekstrak Daun Binahong terhadap Pertumbuhan Candida albicans. Fakumi Med J. 2021;1(2):144–51. DOI: https://doi.org/10.33096/fmj.v1i2.153
Hossain TJ. Methods for screening and evaluation of antimicrobial activity : A review of protocols , advantages , and limitations. 2024;14(2):97–115. DOI: https://doi.org/10.1556/1886.2024.00035
Binter wisda lumban gaol kardo. Aktivitas Antijamur Ekstrak Etanol Daun Daruju (Acanthus ilicifolius Linn.) TERHADAP Trichophyton rubrum. J Compr Sci. 2025;4(4):1480–97. DOI: https://doi.org/10.59188/jcs.v4i4.3105