Physicochemical and Antioxidant Activity Evaluation of Yellow Rope (Anamirta cocculus (L.) Wight & Arn.) Stem Extract Isotonic Drink
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Abstract
as having potential as a natural antioxidant agent. One potential way to utilize this plant is by innovating it into an isotonic drink product. Objective: This study aimed to evaluate the physicochemical properties and antioxidant activity of an isotonic drink formulated with yellow rope stem extract. Methods: he extraction process was conducted using the maceration method with 70% ethanol as the solvent. The isotonic drink was developed into three formulas, which were then tested for their physicochemical properties and antioxidant activity using the DPPH method. Results: The results indicated that the best formula was FII, with an overall hedonic score of 4,6 (neutral). All three formulas showed identical pH values (4,0) and Total Soluble Solids (TSS) of 7%, while the total acidity decreased progressively: FI (1,024%), FII (0,896%), and FIII (0,704%). The isotonic drinks from yellow rope stem extract for formulas FI, FII, and FIII produced IC50 values of 35,61, 46,56, and 57,69 μg/mL, respectively. Based on these data, FI and FII were identified as having very strong antioxidant intensity, while the FIII showed activity classified in the strong category. Conclusion:.The test results demonstrate that the isotonic drink made from yellow rope stem extract exhibits antioxidant potential with intensity levels ranging from strong to very strong.
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References
Adrian, A., Syahputra, R.A., Juwita, N.A., Astyka, R., Lubis, M.F. Andaliman (Zanthoxylum Acanthopodium DC.) a Herbal Medicine from North Sumatera, Indonesia: Phytochemical and Pharmacological Review. Heliyon 2023, 9.
Khayati, L., Noya, J., Benu, N.M.H., Jontara, F. Medicinal Plants in Inamberi Research Station of Manokwari and Their Conservation Status. J. Sylva Indones. 2023, 06, 184–194.
Erawati, R.; Muslihin; Hardia, L. Uji Aktivitas Antioksidan Fraksi Ekstrak Etanol Tali Kuning (Anamirta cocculus) Dengan Metode DPPH. J. Promot. Prev. 2024, 7, 381–391.
Wibawa, J.C., Wati, L.H., Arifin, M.Z. Mekanisme Vitamin C Menurunkan Stres Oksidatif Setelah Aktivitas Fisik. JOSSAE J. Sport Sci. Educ. 2020, 5, 57.
Zenita, R., Yuniarti, E. Analisis Bibliometrik Buah Bit (Beta vulgaris L.) Untuk Antioksidan. J. Pendidik. Biol. 2025, 10, 1370–1377.
Hardiansyah, L.O., Muslihin, A.M., Astuti, R.A. Studi In Vitro Ekstrak Kulit Batang Tali Kuning (A. cocculus) Sebagai Antioksidan. J. Kesehat. Tambusai 2024, 5, 12785–12792.
Masitoh, R., Muslihin, A.M., Budiyanto, A.B. Uji Aktivitas Antioksidan Ekstrak Etanol Tali Kuning (Anamirta cocculus). MIDWINERSLION J. Kesehat. STIKes Buleleng Vol. 2024.
Hasan, H., Thomas, N.A., Hiola, F., Ramadhani, F.N., Ibrahim, P.A.S. Skrining Fitokimia Dan Uji Aktivitas Antioksidan Kulit Batang Matoa (Pometia pinnata) Dengan Metode 1,1-Diphenyl-2 Picrylhidrazyl (DPPH). Indones. J. Pharm. Educ. 2022, 2, 67–73, doi:10.37311/ijpe.v2i1.10995.
Dewi, Y.S.K., Purwayantie, S., Sutignya, T.C.W.A. Teknologi Produksi Isotonik Kaya Antioksidan Berbasis Lidah Buaya-Liang Teh-Madu Hutan. Pros. Saintek 2021, 3, 585–592.
Tumpu, D., Muslihin, A.M., Hardia, L. In Vitro Study of The Activity of Yellow Rope (Anamirta cocculus) Extract As An Antibacterial. JPPIPA J. Penelit. Pendidik. IPA 2025, 11, 382–387.
Muslihin, A.M., Hardia, L., Maulana, F. Antianemic Activity of Anamirta cocculus Hydro-Ethanol Extract against Sodium Nitrite-Induced Anemia in Rats. Trop. J. Nat. Prod. Res. 2025, 9.
Kusuma, A.E., Aprileili, D.A. Pengaruh Jumlah Pelarut Terhadap Rendemen Ekstrak Daun Katuk (Sauropus androgynus L. Merr). Sitawa J. Farm. Sains dan Obat Tradis. 2022, 1, 125–135.
Utami, Y., Julianti, E., Nurminah, M. Formulasi Ekstrak Bunga Telang Dan Ekstrak Kayu Manis Terhadap Karakteristik Fisik Dan Sensori Minuman Isotonik. J. Agroteknologi 2023, 17, 40–52.
Pakaya, S.W., Antuli, Z.A.K., Une, S. Karakteristik Kimia Minuman Isotonik Berbahan Baku Air Kelapa (Cocos nucifera) Dan Ekstrak Jeruk Lemon (Citrus limon) Sukma. Jambura J. Food Technol. 2021, 3, 102–111.
Handayani, F., Sundu, R., Sari, R.M. Formulasi Dan Uji Aktivitas Antibakteri Streptococcus mutans Dari Sediaan Mouthwash Ekstrak Daun Jambu Biji (Psidium guajava L.). J. Sains dan Kesehat. 2017, 1, 422–433.
Aprilia, A., Maherawati, M., Dewi, Y.S.K. Effect of Formulations and Sweetener Type to Characteristic of Coconut-Pineapple Isotonic Drink. Agritekno J. Teknol. Pertan. 2023, 12, 40–49.
Pujiastuti, E., Islamiyati, R. Aktivitas Antioksidan Fraksi Etil Asetat Dan Air Ranting Buah Parijoto (Medinilla speciosa Blume) Dengan Peredaman Radikal Bebas DPPH. Cendekia J. Pharm. 2021, 5, 135–144.
Susiloningrum, D., Sari, D.E.M. Uji Aktivitas Antioksidan Dan Penetapan Kadar Flavonoid Total Ekstrak Temu Mangga (Curcuma mangga Valeton & Zijp) Dengan Variasi Konsentrasi Pelarut. Cendekia J. Pharm. 2021, 5, 117–127.
Ulfa, R.A., Saepuloh, A., Cahyanto, T., Darniwa, A.V., Adawiyah, A. Pengaruh Jenis Pemanis Terhadap PH Dan Aktivitas Antioksidan Sirup Pucuk Mangga (Mangifera indica). Teknol. PANGAN Media Inf. dan Komun. Ilm. Teknol. Pertan. 2022, 13, 76–83.
Sipahelut, S.G., Maitimu, C.V., Rejeki, S., Patty, J.A. Pemanfaatan Sari Buah Tomi-Tomi (Flacourtia inermis Roxb) Sebagai Flavoring Agent Dalam Pembuatan Minuman Isotonik Air Kelapa. J. Teknol. Has. Pertan. 2024, 17, 12–22.
Kementerian Kesehatan RI. Farmakope Herbal Indonesia. Edisi II. Jakarta: Kementerian Kesehatan Republik Indonesia; 2017.
Arifin, J.F.N.; Devitri, R.W.; Septiarini, R.T.; Silvany, E.; Maulidya, V.; Maulidya, Y.E.; Rahmawati, E.E.; Sabila, Z. Tinjauan Literatur: Efektivitas Maserasi Sebagai Metode Ekstraksi Fitokimia. Obat J. Ris. Ilmu Farm. dan Kesehat. 2025, 3, 228–242.
Zhang, Q.W.; Lin, L.G.; Ye, W.C. Techniques for Extraction and Isolation of Natural Products : A Comprehensive Review. Chin. Med. 2018, 13, 1–26.
Sari, P.R.; Astuti, N.B.; Kristanto, B.; Lusiana, S.A.; Gizi, J.; Kemenkes, P.; Gizi, J.; Kemenkes, P.; Wuluh, B.; Karoten, B.; et al. Kadar Beta Karoten Dan Tingkat Kesukaan Sirup Ubi Jalar Kuning (Ipomea batatas L.) Dengan Penambahan Sari Belimbing Wuluh (Averrhoa bilimbi L.). Gema Kesehat. 2023, 15, 110–119.
Sahariah, S.; Sukainah, A.; Hambali, A. Pengaruh Penambahan Sukrosa Dalam Air Kelapa Tua Pada Formulasi Minuman Isotonic Kelapa (Cocos nucifera L). JIMU J. Ilm. Multi Disiplin 2025, 03, 338–353.
Marhamah, I.H. Aktivitas Antimalaria Tanaman Tali Kuning (Anamirta cocculus) Terhadap Plasmodium Sp. J. Ilmu Kedokt. dan Kesehat. 2019, 6, 66–74.
Ishak, I.; Jura, M.R.; Said, I.; Pulukadang, S.H.V. Tingkat Kesadahan Dan Uji Derajat Keasaman (PH) Pada Air Tanah Di Desa Mapane Tambu Kecamatan Balaesang Kabupaten Donggala. Media Eksakta 2022, 18, 102–106.
Badan Standardisasi Nasional. Standar Nasional Indonesia (SNI): Minuman Isotonik SNI 01-4452-1998. Jakarta: Badan Standardisasi Nasional; 1998.
Satria, N.I., Maherawati, M., Fadly, D. Karakteristik Minuman Isotonik Berbahan Baku Air Kelapa (Cocos nucifera) Dengan Penambahan Buah-Buahan Lokal. J. Teknol. dan Ind. Pertan. Indones. 2024, 16, 78–85.
Fitriani, L.N. Analisis Dan Penetapan Kadar Alkaloid Total Pada Ekstrak Etanol Biji Kopi Arabika (Coffea arabica) Dengan Metode KLT-Densitometri, Universitas dr. Soebandi, 2023.
Prayoga, D.G.E., Nocianitri, K.A., Puspawati, N.N. Antioksidan Ekstrak Kasar Daun Pepe (Gymnema reticulatum Br.) Pada Berbagai Jenis Pelarut. J. Ilmu dan Teknol. Pangan 2019, 8, 111–121.
Winahyu, D.A., Retnaningsih, A., Aprillia, M. Determination Of Flavonoid Levels In Raru Wood Stone (CotylelobiummelanoxylonP) With Method UV-Vis Spectrofotometry. J. Anal. Farm. 2019, 4, 29–36.
Garjonyte, R., Budiene, J., Labanauskas, L., Judzentiene, A. In Vitro Antioxidant and Prooxidant Activities of Red Raspberry (Rubus Idaeus L.) Stem Extracts. Molecules 2022, 27, 1–19.
Oktaria, D., Marpaung, M.P. Penetapan Kadar Flavonoid Total Dan Aktivitas Antioksidan Ekstrak Akar Nipah (Nypa fruticans Wurmb) Dengan Metode Spektrofotometri UV-Vis. Lantania J. 2023, 11, 36–50.
Hamdan, M. Uji Aktivitas Antioksidan Ekstrak Etanol Daun Beluntas (Pluchea indica L.) Dengan Metode DPPH, Universitas dr. Soebandi, 2022.