In Vitro Study : Antifungal Activity of Coffea canephora Peel and Leaf Extracts Against Candida albicans
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Abstract
Candida albicans is a major cause of invasive candidiasis with high morbidity and mortality rates, and it has shown increasing resistance to antifungal agents. The objective of this research to evaluate the antifungal activity of Coffea canephora peel and leaf extract against Candida albicans in vitro by determining the Minimum Inhibitory Concentration (MIC). Robusta coffee peel and leaves were extracted using maceration with 96% ethanol, followed by phytochemical screening and antifungal activity testing using the broth dilution method. Phytochemical screening revealed the presence of alkaloids, phenolic-tannins, flavonoids, saponins, and terpenoids, while steroid were not detected. Based on the antifungal activity test conducted separately, both extracts had the same MIC value at a concentration of 12,5%. Statistical analysis using One Way ANOVA demonstrated a significant difference among treatment groups (p<0,05). The antifungal activity of the extract is presumed to be associated with phenolic compounds, such as chlorogenic acid and caffeine, which may act through disruption of cell membrane integrity and inhibition of ergosterol synthesis. These finding suggest that Coffea canephora peel and leaf extract has the potential to be further developed as an antifungal agent, especially in topical formulations, but further toxicity tests and in vivo tests are required.
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References
Srivastava, Vartika; Singla, Rajeev Kumar; Dubey AK. Emerging Virulence, Drug Resistance and Future Anti-fungal Drugs for Candida Pathogens. Curr Top Med Chem 2018;18:759–78. https://doi.org/10.2174/1568026618666180528121707.
Katsipoulaki M, Stappers MHT, Malavia-jones D, Brunke S, Hube B, Gow NAR. Candida albicans and Candida glabrata: global priority pathogens. vol. 0. American Society for Microbiology; 2024.
Talapko J, Juzbašić M, Matijević T, Pustijanac E, Bekić S, Kotris I, et al. Candida albicans-the virulence factors and clinical manifestations of infection. Journal of Fungi 2021;7:1–19. https://doi.org/10.3390/jof7020079.
Lu H, Shrivastava M, Whiteway M, Jiang Y. Candida albicans targets that potentially synergize with fluconazole. Crit Rev Microbiol 2021;47:323–37. https://doi.org/10.1080/1040841X.2021.1884641.
Lee Y, Puumala E, Robbins N, Cowen LE. Antifungal Drug Resistance: Molecular Mechanisms in Candida albicans and beyond. Chem Rev 2021;121:3390–411. https://doi.org/10.1021/acs.chemrev.0c00199.
Anjani G, Widyastuti N, Masruroh Z, Yuliana RAD, Almira VG, Tsani AFA, et al. Bioactive components and antibacterial activity in robusta coffee leaves (Coffea canephora). International Journal of Pharmaceutical Research 2020;12:1374–82.
Widianingsih M. Daun dan Kulit Buah Kopi Robusta ( Coffea canephora Pierre ex A . Froehner ) Sebagai Sumber Senyawa Bioaktif untuk Aplikasi Farmakologi. Borneo Journal Of Biology Education 2025;7:140–8.
Castro-Díaz R, Silva-Beltrán NP, Gámez-Meza N, Calderón K. The Antimicrobial Effects of Coffee and By-Products and Their Potential Applications in Healthcare and Agricultural Sectors: A State-of-Art Review. Microorganisms 2025;13:1–20. https://doi.org/10.3390/microorganisms13020215.
Calheiros D, Dias MI, Calhelha RC, Barros L, Ferreira ICFR, Fernandes C, et al. Antifungal Activity of Spent Coffee Ground Extracts. Microorganisms 2023;11:1–20. https://doi.org/10.3390/microorganisms11020242.
Kurnia S, Zasari M. Karakterisasi Morfologi Tanaman Kopi Rakyat di Pulau Bangka ( Morphological Characterization of Small Holder Plantations in Bangka Island ). Jurnal Agro Industri Perkebunan 2023;11:115–32.
Saputra IKA, Ermayanti NGAM, Sukmaningsih AASA. Pengaruh Ekstrak Daun Kopi Robusta (Coffea canephora Pierre ex A. Froehner) terhadap Kualitas Spermatozoa Mencit (Mus musculus L.) Yang Terpapar Asap Rokok. Biosaintropis (Bioscience-Tropic) 2021;7:74–84. https://doi.org/10.33474/e-jbst.v7i1.445.
Nintowati PD. Analisis Fitokimia Dan Aktivitas Antioksidan Daun Kopi Robusta ( Coffea canephora ) Berdasarkan Perbedaan Pendahuluan Kopi Robusta ( Coffea canephora ) merupakan salah satu jenis kopi yang banyak dibudidayakan di Indonesia dan menjadi salah satu komoditas. Jurnal Ilmiah Biologi 2024;12:2317–33.
EUCAST. EUCAST definitive document EDef 7.1: Method for the determination of broth dilution MICs of antifungal agents for fermentative yeasts. Clinical Microbiology and Infection 2008;14:398–405. https://doi.org/10.1111/j.1469-0691.2007.01935.x.
Tivani, Inur; Amananti W. Uji Efektivitas Antifungi Perasan Daun Turi (Sesbania grandiflora (L.) Pers.) terhadap Jamur Candida albicans The. Pharmacy: Jurnal Farmasi Indonesia 2020;17:35–41.
Br Situmorang, Ing Mayfa; Salsadila, Putri; Maulidayanti, Sharfina; Tanjung A. Aktivitas Antijamur Ekstrak Biji Kopi Hijau Robusta (Coffea canephora) Terhadap Candida albicans. Pharmaceutical and Biomedical Sciences Journal (PBSJ) 2024;6:136–43. https://doi.org/https://doi.org/10.15408/pbsj.v6i2.39149.
CLSI. CLSI M27 Reference Method for Broth Dilution Antifungal Susceptibility Testing of Yeasts. Clinical and Laboratory Standards Institute 2017. https://clsi.org/shop/standards/m27/.
Rosa Y, Riyanto R. Potential of Robusta Coffee Bean Extract (Coffea canephora) Peaberry Roasted and Green Bean Pagar Alam City against the Growth of Candida albicans Fungus. Jurnal Biologi Tropis 2022;22:1108–14. https://doi.org/10.29303/jbt.v22i4.4311.
Aldholmi M, Marchand P, Ourliac-Garnier I, Le Pape P, Ganesan A. A decade of antifungal leads from natural products: 2010–2019. Pharmaceuticals 2019;12:2010–9. https://doi.org/10.3390/ph12040182.
Harborne JB. Phytochemical methods. In: A guide to modern techniques of plant analysis. Third Edit. Chapman & Hall; 1998.
Akinmoladun AC, Falaiye OE, Ojo OB, Adeoti A, Amoo ZA, Olaleye MT. Effect of extraction technique, solvent polarity, and plant matrix on the antioxidant properties of Chrysophyllum albidum G. Don (African Star Apple). Bull Natl Res Cent 2022;46. https://doi.org/10.1186/s42269-022-00718-y.
Chen Z, Shen N, Wu X, Jia J, Wu Y, Chiba H, et al. Extraction and Quantitation of Phytosterols from Edible Brown Seaweeds: Optimization, Validation, and Application. Foods 2023;12. https://doi.org/10.3390/foods12020244.
Balouiri M, Sadiki M, Ibnsouda SK. Methods for in vitro evaluating antimicrobial activity: A review. J Pharm Anal 2016;6:71–9. https://doi.org/10.1016/j.jpha.2015.11.005.
Ahmad I, Ambarwati NSS, Lukman A, Masruhim MA, Rijai L, Mun’Im A. In vitro antimicrobial activity evaluation of mangrove fruit (Sonneratia caseolaris L.) extract. Pharmacognosy Journal 2018;10:598–601. https://doi.org/10.5530/pj.2018.3.98.
Ali A, Parisi A, Normanno G. Polyphenols as Emerging Antimicrobial Agents. Emerging Modalities in Mitigation of Antimicrobial Resistance, Cham: Springer International Publishing; 2022, p. 219–59. https://doi.org/10.1007/978-3-030-84126-3_10.
Simonetti G, Brasili E, Pasqua G. Antifungal Activity of Phenolic and Polyphenolic Compounds from Di ff erent Matrices of Vitis vinifera. Molecules 2020;25:1–22.
Campoy S, Adrio JL. Antifungals. Biochem Pharmacol 2017;133:86–96. https://doi.org/10.1016/j.bcp.2016.11.019.
Bhattacharya S, Sae-Tia S, Fries BC. Candidiasis and mechanisms of antifungal resistance. Antibiotics 2020;9:1–19. https://doi.org/10.3390/antibiotics9060312.
Davidova S, Galabov AS, Satchanska G. Antibacterial, Antifungal, Antiviral Activity, and Mechanisms of Action of Plant Polyphenols. Microorganisms 2024;12. https://doi.org/10.3390/microorganisms12122502.
Girardot M, Millot M, Hamion G, Billard JL, Juin C, Ntoutoume GMAN, et al. Lichen Polyphenolic Compounds for the Eradication of Candida albicans Biofilms. Front Cell Infect Microbiol 2021;11:1–10. https://doi.org/10.3389/fcimb.2021.698883.
Karpiński TM, Ożarowski M, Seremak-Mrozikiewicz A, Wolski H, Adamczak A. Plant preparations and compounds with activities against biofilms formed by candida spp. Journal of Fungi 2021;7:1–13. https://doi.org/10.3390/jof7050360.
Gholam GM, Darmawan NI, Siregar JE, Artika IM. Selected Polyphenols from Date (Phoenix dactylifera) as Anti-Virulence of Candida albicans Through Multiple Enzyme Targets. Biointerface Res Appl Chem 2023;13. https://doi.org/10.33263/BRIAC134.386.
Rojas-González A, Figueroa-Hernández CY, González-Rios O, Suárez-Quiroz ML, González-Amaro RM, Hernández-Estrada ZJ, et al. Coffee Chlorogenic Acids Incorporation for Bioactivity Enhancement of Foods: A Review. Molecules 2022;27:1–23. https://doi.org/10.3390/molecules27113400.