The Uji Aktivitas Antibakteri Sediaan Gel Semprot Ekstrak Etanol Daun Sintrong (Crassocephalum crepidiodes) Terhadap Staphylococcus aureus Pada Luka Infeksi Diabetes Mellitus
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Sry Anita Sipayung, Astriani Natalia, Rena Meutia, Nerly Juli Pranita Simanjuntak (2025). The Uji Aktivitas Antibakteri Sediaan Gel Semprot Ekstrak Etanol Daun Sintrong (Crassocephalum crepidiodes) Terhadap Staphylococcus aureus Pada Luka Infeksi Diabetes Mellitus. Journal of Pharmaceutical and Sciences, JPS Volume 8 Nomor 2 (2025). https://doi.org/10.36490/journal-jps.com.v8i2.887
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Latar Belakang: Diabetes mellitus (DM) merupakan kelainan metabolik yang menyebabkan gangguan penyembuhan luka. Luka pada penderita diabetes cenderung sulit sembuh akibat kadar glukosa darah tinggi yang mengganggu sistem imun dan sirkulasi darah. Penggunaan antibiotik jangka panjang dapat menimbulkan resistensi, sehingga diperlukan alternatif antibakteri alami seperti daun sintrong (Crassocephalum crepidioides), yang mengandung flavonoid, polifenol, saponin, dan tanin dengan aktivitas antibakteri. Formulasi ekstrak daun sintrong dalam sediaan gel semprot dipilih karena memberikan keunggulan dalam aplikasi pada luka, termasuk kemudahan penggunaan, sterilitas, dan kemampuan penetrasi yang baik. Tujuan: Penelitian ini bertujuan memformulasi ekstrak etanol daun sintrong menjadi sediaan gel semprot dengan variasi konsentrasi (5%, 10%, 15%, 20%), mengevaluasi stabilitas fisik, serta menguji aktivitas antibakterinya terhadap S. aureus. Metode: Ekstrak daun sintrong diperoleh melalui maserasi etanol 96%, kemudian diformulasikan menjadi gel semprot dengan basis Carbopol 940. Evaluasi stabilitas meliputi uji organoleptik, viskositas, pH, waktu kering, dan pola semprot. Aktivitas antibakteri diuji menggunakan metode difusi cakram. Hasil Penelitian: Gel semprot menunjukkan stabilitas fisik yang baik selama 21 hari penyimpanan. Uji antibakteri menghasilkan zona hambat dengan diameter 8,05 mm (5%), 8,02 mm (10%), 8,44 mm (15%), dan 9,57 mm (20%), tergolong efektivitas sedang. Konsentrasi 20% memberikan efek penghambatan tertinggi, meskipun masih di bawah kontrol positif (klindamisin 1%). Kesimpulan: Ekstrak etanol daun sintrong dapat diformulasikan sebagai gel semprot yang stabil dan memiliki aktivitas antibakteri terhadap S. aureus, dengan efektivitas tertinggi pada konsentrasi 20%. Penelitian ini memberikan alternatif potensial untuk pengobatan infeksi luka diabetik berbahan alam.
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Referensi
Poznyak A, Grechko A V, Poggio P, Myasoedova VA, Alfieri V, Orekhov AN. The diabetes mellitus–atherosclerosis connection: The role of lipid and glucose metabolism and chronic inflammation. Int J Mol Sci 2020;21:1835.
World Health Organization. Diabetes. World Heal Organ 2022. https://www.who.int/news-room/fact-sheets/detail/diabetes.
Atlas D. International diabetes federation. IDF Diabetes Atlas, 7th Edn Brussels, Belgium Int Diabetes Fed 2015;33.
Magliano DJ, Boyko EJ. IDF diabetes atlas 2022.
Ogurtsova K, Guariguata L, Barengo NC, Ruiz PL-D, Sacre JW, Karuranga S, et al. IDF diabetes Atlas: Global estimates of undiagnosed diabetes in adults for 2021. Diabetes Res Clin Pract 2022;183:109118.
Rizqiyah H, Soleha TU, Hanriko R, Apriliana E. Pola Bakteri Ulkus Diabetikum Pada Penderita Diabetes Melitus di RSUD dr. H. Abdul Moeloek. Majority 2020;9:128–35.
Sukartini T, Theresia Dee TM, Probowati R, Arifin H. Behaviour model for diabetic ulcer prevention. J Diabetes Metab Disord 2020;19:135–43. https://doi.org/10.1007/s40200-019-00484-1.
Razoki R, Simanjuntak NJP. Potensi Antihiperglikemia Kombinasi Ekstrak Daun Salam dan Ikan Gabus. J Indah Sains Dan Klin 2024;5:19–23. https://doi.org/10.52622/jisk.v5i3.04.
Panji Ratih Suci, Cikra Ikhda Nur Hamidah Safitri, Nisa’ul Choiroh. Uji Aktivitas Antibakteri Ekstrak Daun Sintrong (Crassocephalum crepidioides Benth. S. Moore) Pada Salmonella typhi. AFAMEDIS 2020;1:1–10. https://doi.org/10.61609/afamedis.v1i2.21.
Maimunah S, Pratama HA, Mayasari U. Uji Aktivitas Antibakteri Ekstrak Daun Sintrong (Crassocephalum crepidiodies) Terhadap Bakteri Staphylococcus aureus|| Antibacterial Activity Assay From Sintrong Leaf (Crassocephalum crepidiodies) Against Staphylococcus aureus Bacteria. J Pembelajaran Dan Biol Nukl 2020;6:103–11.
Indalifiany A, Zubaydah WS, Kasim ER. Formulasi Spray Gel Ekstrak Etanol Batang Etlingera Rubroloba Menggunakan HPMC Sebagai Gelling Agent: Formulation Spray Gel Of Etlingera Rubroloba Ethanolic Extract Using HPMC As Gelling Agent. J Sains Dan Kesehat 2023;5:140–8.
Anindhita MA, Oktaviani N. Formulasi spray gel ekstrak daun pandan wangi sebagai antiseptik tangan. Parapemikir J Ilm Farm 2020;9:14–21.
Zubaydah WOS, Aspadiah V, Muammar M. Pengembangan Sediaan Spray Gel dari Ekstrak Etanol Batang Tumbuhan Bambu-bambu (Polygonum pulchurm Blume) Menggunakan Basis Kombinasi Gel Viskolam® dan Hydroxypropyl Methyl Cellulose (HPMC). Medula 2022;10:53–65.
Estikomah SA, Amal ASS, Safaatsih SF. Uji daya hambat terhadap bakteri Staphylococcus aureus, Staphylococcus epidermidis, Propionibacterium acnes gel semprot ekstrak etanol daun kersen (Muntingia calabura L.) Karbopol 940. Pharm J Islam Pharm 2021;5:36–53.
QUR’AN SCNUR. Uji Aktivitas Antibakteri Fraksi Ekstrak Daun Eceng Gondok (Eichhornia crassipes) Terhadap Bakteri Staphylococcus aureus 2020.
Usman Y. Pemanfaatan potensi limbah kulit bawang merah (Allium cepa. l) sebagai sediaan gel hand sanitizer. J Ris Kefarmasian Indones 2020;2:63–71.
Prasetya I, Putra GG, Wrasiati LP. Pengaruh Jenis Pelarut dan Waktu Maserasi terhadap Ekstrak Kulit Biji Kakao (Theobroma Cacao L..) sebagai Sumber Antioksidan. J Rekayasa Dan Manaj Agroindustri ISSN 2020;2503:150–9.
Rizal R, Maharani V. Formulasi sediaan spray gel ekstrak etanol pegagan (Centella asiatica (L.) Urban) dan uji daya tabir surya. J Sains Farm Dan Kesehat 2023;1:48–59.
Utami CA. Formulasi Spraygel Minyak Atsiri Daun Seledri (Apium Graveolens L.) Dan Uji Aktivitas Antibakteri Terhadap Staphylococcus aureus ATCC 25923. Univ Islam Indones 2020.
Tanjung C, Al Fajr IT, Khoiriyah I, Senjaya MRH, Nabila SP, Akmal T. Formulasi dan Evaluasi Sediaan Spray gel Minyak Atsiri Niaouli (Melaleuca quinquenervia L.) dengan Karbopol 940 sebagai Pembentuk Gel. Pharm Sci Clin Pharm 2024;2:1–6.
Humphries RM, Kircher S, Andrea F, Krause KM, Malherbe R, Hsiung A, et al. The Continued Value of Disk Diffusion for Assessing Antimicrobial Susceptibility in Clinical Laboratories: Report From the Clinical and Laboratory Standards Institute Methods Development and Standardization Working Group. J Clin Microbiol 2018;56. https://doi.org/10.1128/jcm.00437-18.
Nurhayati LS, Yahdiyani N, Hidayatulloh A. Perbandingan pengujian aktivitas antibakteri starter yogurt dengan metode difusi sumuran dan metode difusi cakram. J Teknol Has Peternak 2020;1:41–6.
Pisacha IM, Safutri W, Rahayu KW. Review Artikel: Aktivitas Antibakteri Ekstrak Bunga telang (Clitoria ternatea L.) terhadap Bakteri Staphylococcus aureus. J Farm Univ Aisyah Pringsewu 2023;2:70.
Ernawati E, Jannah N. Aktivitas antimikroba perasan daun kirinyuh (Chromolaena odorata L.) terhadap Candida albicans dan Pseudomonas aeruginosa. J Kedokt Dan Kesehat 2021;17:137–44.
Matondang FH, Lubis MS, Yuniarti R, Rani Z. Formulasi masker wajah serbuk nano teh celup bekas dan aktivitas antibakteri terhadap Propionibacterium acnes dan Staphylococcus epidermidis. J Pharm Sci 2025:1–21.
Hombach M, Ochoa C, Maurer FP, Pfiffner T, Böttger EC, Furrer R. Relative Contribution of Biological Variation and Technical Variables to Zone Diameter Variations of Disc Diffusion Susceptibility Testing. J Antimicrob Chemother 2015;71:141–51. https://doi.org/10.1093/jac/dkv309.
Liu Y, Ding L, Han R, Zeng B, Li J, Guo Y, et al. Assessment of Cefiderocol Disk Diffusion Versus Broth Microdilution Results When Tested Against Acinetobacter Baumannii Complex Clinical Isolates. Microbiol Spectr 2023;11. https://doi.org/10.1128/spectrum.05355-22.
Chandrasekaran S, Abbott AN, Campeau S, Zimmer B, Weinstein MP, Thrupp L, et al. Direct-From-Blood-Culture Disk Diffusion to Determine Antimicrobial Susceptibility of Gram-Negative Bacteria: Preliminary Report From the Clinical and Laboratory Standards Institute Methods Development and Standardization Working Group. J Clin Microbiol 2018;56. https://doi.org/10.1128/jcm.01678-17.
Lehtopolku M, Kotilainen P, Puukka P, Nakari U, Siitonen A, Eerola E, et al. Inaccuracy of the Disk Diffusion Method Compared With the Agar Dilution Method for Susceptibility Testing of Campylobacter SPP. J Clin Microbiol 2012;50:52–6. https://doi.org/10.1128/jcm.01090-11.
Humphries RM, Ambler J, Mitchell SL, Castanheira M, Dingle T, Hindler JA, et al. CLSI methods development and standardization working group best practices for evaluation of antimicrobial susceptibility tests. J Clin Microbiol 2018;56:10–1128.
Humphries RM, Ambler J, Mitchell SL, Castanheira M, Dingle T, Hindler JA, et al. on behalf of the CLSI Methods Development and Standardization Working Group of the Subcommittee on Antimicrobial Susceptibility Testing. 2018. CLSI Methods Development and Standardization Working Group best practices for evaluation of antimicrobial suscep. J Clin Microbiol 2018;56:1934.
Cushnie T, Lamb AJ. Antimicrobial activity of flavonoids International Journal of Antimicrobial Agents 26 (5): 343-56 2005.
Haslam E. Natural polyphenols (vegetable tannins) as drugs: possible modes of action. J Nat Prod 1996;59:205–15.
Hemaiswarya S, Kruthiventi AK, Doble M. Synergism between natural products and antibiotics against infectious diseases. Phytomedicine 2008;15:639–52.
Cushnie TPT, Lamb AJ. Recent Advances in Understanding the Antibacterial Properties of Flavonoids. Int J Antimicrob Agents 2011;38:99–107. https://doi.org/10.1016/j.ijantimicag.2011.02.014.
Wu T, Zang X, He M, Pan S, Xu X. Structure–Activity Relationship of Flavonoids on Their Anti-Escherichia Coli Activity and Inhibition of DNA Gyrase. J Agric Food Chem 2013;61:8185–90. https://doi.org/10.1021/jf402222v.
Rodríguez B, Pacheco LGC, Bernal I, Pina M. Mechanisms of Action of Flavonoids: Antioxidant, Antibacterial and Antifungal Properties. Cienc Ambient Y Clima 2023;6:33–66. https://doi.org/10.22206/cac.2023.v6i2.3021.
Xie Y, Yang W, Tang F, Chen X, Ren L. Antibacterial activities of flavonoids: structure-activity relationship and mechanism. Curr Med Chem 2015;22:132–49.
Xie Y, Yang W, Tang F, Chen X, Chen L. Antibacterial Activities of Flavonoids: Structure-Activity Relationship and Mechanism. Curr Med Chem 2014;22:132–49. https://doi.org/10.2174/0929867321666140916113443.
Khan MI, Ahhmed AM, Shin JH, Baek JS, Kim MY, Kim JD. Green Tea Seed Isolated Saponins Exerts Antibacterial Effects Against Various Strains of Gram Positive and Gram Negative Bacteria, a Comprehensive Study In Vitro and In Vivo. Evidence-Based Complement Altern Med 2018;2018. https://doi.org/10.1155/2018/3486106.
Zhang R, Du Z, Li Z, Feng Y, Yan X. Improving the Bioactivity of Water‐soluble Alfalfa Saponins Using Biotransformation. J Food Sci 2024;89:8628–43. https://doi.org/10.1111/1750-3841.17523.
Grzywaczyk A, Smułek W, Olejnik A, Guzik U, Nowak A, Kaczorek E. Co-Interaction of Nitrofuran Antibiotics and the Saponin-Rich Extract on Gram-Negative Bacteria and Colon Epithelial Cells. World J Microbiol Biotechnol 2023;39. https://doi.org/10.1007/s11274-023-03669-2.
Sudji IR, Subburaj Y, Frenkel N, García‐Sáez AJ, Wink M. Membrane Disintegration Caused by the Steroid Saponin Digitonin Is Related to the Presence of Cholesterol. Molecules 2015;20:20146–60. https://doi.org/10.3390/molecules201119682.
Gordon NC, Wareham DW. Antimicrobial Activity of the Green Tea Polyphenol (−)-Epigallocatechin-3-Gallate (EGCG) Against Clinical Isolates of Stenotrophomonas Maltophilia. Int J Antimicrob Agents 2010;36:129–31. https://doi.org/10.1016/j.ijantimicag.2010.03.025.
Shamsudin NF, Ahmed QU, Mahmood S, Shah SAA, Khatib A, Mukhtar S, et al. Antibacterial Effects of Flavonoids and Their Structure-Activity Relationship Study: A Comparative Interpretation. Molecules 2022;27:1149. https://doi.org/10.3390/molecules27041149.
Górniak I, Bartoszewski R, Króliczewski J. Comprehensive Review of Antimicrobial Activities of Plant Flavonoids. Phytochem Rev 2018;18:241–72. https://doi.org/10.1007/s11101-018-9591-z.