Phytochemical Screening and Antioxidant Activity Test of Ethanol Extract of Ciplukan Leaves (Physalis angulata L) in the Seulawah Agam Geothermal Manifestation Area
Article Sidebar
Main Article Content
Page: 571-580
Abstract
Ciplukan leaves (Physalis angulata L.) from the Seulawah Agam geothermal manifestation area exhibit strong potential as a natural antioxidant source. This study investigates the phytochemical content and antioxidant activity of ethanol extract from ciplukan leaves using UV-Vis spectrophotometry. The research begins with sample collection and plant determination, followed by phytochemical screening and antioxidant activity testing using the DPPH method. Phytochemical screening results reveal the presence of alkaloids, flavonoids, tannins, saponins, and steroids. Antioxidant activity testing using the DPPH method yields an IC50 value of 14.99 μg/mL, significantly lower than the positive control, ascorbic acid (19.40 μg/mL), indicating strong antioxidant activity. At the highest tested concentration (50 μg/mL), the extract exhibits an inhibition percentage of 81.84%, while at the lowest concentration (10 μg/mL), it shows an inhibition of 41.41%. These findings confirm that the ethanol extract of ciplukan leaves from the geothermal area possesses high antioxidant content and has great potential for applications in the development of natural-based pharmaceutical and nutraceutical products.
Downloads
Article Details
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
References
Fadhli H, Shinta LS, Mustika F, Wira NS, Emma S, Musyirna RN. Ciplukan (Physalis angulata L.): Review Tanaman Liar yang Berpotensi Sebagai Tanaman Obat. Jurnal Farmasi Indonesia. 2023;15(2):134-41. DOI: https://doi.org/10.35617/jfionline.v15i2.144
Rahayu SR, Diarti MW. Uji daya hambat filtrat daun ciplukan (Physalis angulata L.) terhadap pertumbuhan bakteri Staphylococcus aureus. Jurnal Analisis Medika Bio Sains. 2018;5(1):101-6. DOI: https://doi.org/10.32807/jambs.v5i2.113
Fitri NL, Susetyarini RE, Waluyo L. Pengaruh Ekstrak Buah Ciplukan (Physalis angulata L.) Terhadap Kadar SGPT dan SGOT Mencit Putih Jantan. [Jurnal tidak disebutkan]. 2016.
Ghani NFS. Aktivitas Antioksidan Ekstrak Dan Fraksi Herba Ciplukan (Physalis angulata) Terhadap DPPH (1,1-Difenil-2-Pikrilhidrazil). Prosiding Seminar Nasional Unimus. 2018;1. [Internet]. Available from: http://prosiding.unimus.ac.id/index.php/semnas/article/view/56vol.1 [Diakses 6 Maret 2024].
Hendra PM. Skrining Fitokimia dan Potensi Antidiabetes Ekstrak Etanol Herba Ciplukan (Physalis angulata L.) pada Tikus Putih (Rattus norvegicus) yang Diinduksi Aloksan. BIOEDU. 2019;4(3):90-8. DOI: https://doi.org/10.32938/jbe.v4i3.422
Iswahyudi I, Luliana S, Riza H. Analisis Fitokimia Dan Profil Kromatografi Tipis Ekstrak Etanol Daun Ciplukan (Physalis angulata L.) dengan Berbagai Metode Pengeringan Simplisia. Jurnal Farmasi. 2014;11(4):11-21.
Rohayani IS, Aryanti E, Suripto. Kandungan Fitokimia Beberapa Jenis Tumbuhan Lokal yang Sering Dimanfaatkan Sebagai Bahan Baku Obat di Pulau Lombok. Prosiding Seminar Nasional Masyarakat Biodiversitas Indonesia. 2015;1(2):388-91. DOI: https://doi.org/10.13057/psnmbi/m010237
Syukri M, et al. The Investigation of Hot Spring Flow Using Resistivity Method at Geothermal Field Ie-Seu’um, Aceh – Indonesia. Bund. K. 2014;19:2420.
Nuraskin C, Marlina, Idroes R, Soraya C, Djufri. Identification of Secondary Metabolite of Laban Leaf Extract (Vitex pinnata L.) from Geothermal and Non-Geothermal Areas of Agam Mountains in Aceh Besar, Aceh Province, Indonesia. Rasayan J Chem. 2020;13:18-23. DOI: https://doi.org/10.31788/RJC.2020.1315434
Azhari S, Marniza E, Mahmudi, Farida M. Analisis Kadar Flavonoid Ekstrak Etanol Daun Laban (Vitex pinnata) Kawasan Geothermal dan Non-Geothermal Menggunakan Spektroskopi UV-VIS. Serambi Konstruktivis. 2022;4(2):2656-5781.
Sampaio BL, Edrada-Ebel R, Da Costa FB. Effect of the Environment on the Secondary Metabolic Profile of Tithonia diversifolia: A Model for Environmental Metabolomics of Plants. Sci Rep. 2016;6:1-11. https://doi.org/10.1038/srep29265. DOI: https://doi.org/10.1038/srep29265
Idroes R, Yusuf M, Alatas M, Lala A, Suhendra R, Idroes GM. Geochemistry of Sulphate Spring in the Ie Jue Geothermal Areas at Aceh Besar District, Indonesia. IOP Conf Ser: Mater Sci Eng. 2019;523:012012. DOI: https://doi.org/10.1088/1757-899X/523/1/012012
Effendy E, Respatijarti R, Waluyo B. Keragaman Genetik dan Heritabilitas Karakter Komponen Hasil dan Hasil Ciplukan (Physalis sp.). J Agro. 2018;5(1):30-8. DOI: https://doi.org/10.15575/1864
Rukmi K, Waluyo B. Keragaman Genetik Aksesi Ciplukan (Physalis sp.) Berdasarkan Karakter Morfologi dan Agronomi. J Produksi Tanam. 2019;7(2):209-19.
Jariyah, Widjanarko SB, Yunianta, Estiasih T. Hypoglycemic Effect of Pedada (Sonneratia caseolaris) Fruit Flour (PFF) in Alloxan-Induced Diabetic Rats. Int J PharmTech Res. 2015;7(1):31-40.
Novita SR, Purnamaningsih SL, Khairiyah LL, Waluyo B. Seleksi Kegenjahan dan Hasil Tinggi pada Ciplukan (Physalis angulata L.) Berdasarkan Nilai Kemajuan Genetik. J Pertan Terpadu. 2022;10(1):37-51. DOI: https://doi.org/10.36084/jpt..v10i1.396
Helmi HR, Yulianti E, Malihah E, Elhapidi NZ, Dewi MA, Ferdinal F. Uji Fitokimia, Kapasitas Antioksidan, Uji Toksisitas, Ekstrak Buah Acaiberry (Euterpe oleracea), Ciplukan (Physalis angulata Linn) dan Kurma Ajwa (Phoenix dactylifera L). J Muara Sains, Teknol Kedokteran, dan Ilmu Kesehat. 2020;5(2):361-70. DOI: https://doi.org/10.24912/jmstkik.v5i2.9439
Nuranda A, Saleh C, Yusuf B. Potensi Tumbuhan Ciplukan (Physalis angulata Linn.) sebagai Antioksidan Alami. J At. 2016;1(1):5-9.
Olusola BO, Adeseye R-EI. In Vitro Antioxidant Alpha-amylase and Alpha Glucosidase Inhibitory Properties of Ethanol Extract of Physalis angulata L. In: The Academic Staff Union of Polytechnics (ASUP) ZONE C Virtual Conference. 2020.
Sari NGF. Aktivitas Antioksidan Ekstrak dan Fraksi Herba Ciplukan (Physalis angulata) terhadap DPPH (1,1-Difenil-2-Pikrilhidrazil). Pros Semin Nas Unimus. 2018;1.
Azhari S, Ningsih DS, Nuraskin CA, Karma T, Muslem, Idroes GM, et al. Identification of Geothermal and Non-Geothermal Laban Plant (Vitex pinnata) With a Combination of Infrared Spectroscopy – Principal Component Analysis Methods. Adv Health Sci Res. 2019;32:85-9.
Saidi N, Ginting B. Analisis Metabolit Sekunder. Banda Aceh: Syiah Kuala University Press; 2018.
Hanin NNF, Pratiwi R. Kandungan Fenolik, Flavonoid dan Aktivitas Antioksidan Ekstrak Daun Paku Laut (Acrostichum aureum L.) Fertil dan Steril. J Trop Biodiv Biotech. 2017;2:51-6. DOI: https://doi.org/10.22146/jtbb.29819
Maulana I, Ginting B, Nurdin N, Fakri S. Antioxidant Activity of Ethyl Acetate Extract of Nutmeg (Myristica fragrans Houtt) Stem Bark. Jurnal Natural. 2019;19(3):58-63. DOI: https://doi.org/10.24815/jn.v19i3.12618
Ginting B, Maulana I, Saidi N, Astryna SY. Isolation and Activity Antioxidant Test of Cocoa Pod Husk Ethyl Acetate Extracts (Theobroma cacao L). Jurnal Natural. 2019;19(2):49-53. DOI: https://doi.org/10.24815/jn.v19i2.12568
Masrifah R, Abram HP. Uji Aktivitas Ekstrak Daun dan Kulit Labu Air (Lagenaria siceraria (Molina) Standl). Jurnal Akad Kim. 2017;6(2):96-106. DOI: https://doi.org/10.22487/j24775185.2017.v6.i2.9240
Satria MD, Sari R, Wahdaningsih S. Uji Aktivitas Antioksidan Ekstrak n-Heksan Buah Lakum (Cayratia trifolia) dengan Metode DPPH. Jurnal Ilmiah. 2013;5(3):7.
Endang, Hanani. Analisis Fitokimia. Jakarta: EGC; 2005.
Fitriani, Erlyn. Aktivitas Antidiabetik Kombinasi Ekstrak Etanol Daun Ciplukan (Physalis angulata) dan Daun Gaharu (Aquilaria malaccensis) pada Tikus Diabetes. Syifa Med J Kedokt Kesehat. 2019;9(2). doi:10.32502/sm.v9i2.1660. DOI: https://doi.org/10.32502/sm.v9i2.1660
Hikmah, Anggarani. Kandungan Senyawa Bioaktif dan Aktivitas Antioksidan Bawang Merah Nganjuk (Allium Cepa L.). Unesa J Chem. 2021;10(3):220-30. doi:10.26740/ujc.v10n3.p220-230. DOI: https://doi.org/10.26740/ujc.v10n3.p220-230
Samirana PO, Suartha IN, Dewi NP. Penentuan Profil Bioautografi dan Aktivitas Antioksidan Ekstrak Etanol Daun Bidara (Ziziphus mauritiana Auct. non Lamk.) dengan Metode Penangkapan Radikal DPPH. J Farm Udayana. 2018;6(2). doi:10.24843/jfu.2017.v06.i02.p04. DOI: https://doi.org/10.24843/JFU.2017.v06.i02.p04
Lantah J, Rembet U, Mamuaja J. Kandungan Fitokimia dan Aktivitas Antioksidan Ekstrak Metanol Rumput Laut Kappaphycus alvarezii. Media Teknol Has Perikan. 2017;5(3). doi:10.35800/mthp.5.3.2017.16785. DOI: https://doi.org/10.35800/mthp.5.3.2017.16785
Nadillah Z, Fajriaty I, Wahyuni S. Uji Aktivitas Antioksidan Ekstrak Etil Asetat Daun Cempedak (Artocarpus champaden Spreng.). J Borneo. 2022;2(2). doi:10.57174/jborn.v2i2.40. DOI: https://doi.org/10.57174/jborn.v2i2.40
Cahyono B, Sari RP, Utami ND. Penentuan Aktivitas Antioksidan Senyawa Kuersetin dan Ekstrak Lengkuas Menggunakan HPLC dan UV-Vis. Alchemy J Chem. 2021;8(2). doi:10.18860/al.v8i2.10594. DOI: https://doi.org/10.18860/al.v8i2.10594
Azim MF, Rahmawati SI, Nurdiana A. Aktivitas Antioksidan Buah Sawo Manila (Manilkara zapota L) Sebagai Kandidat Produk Perawatan Kulit. Sinteza. 2022;2(2). doi:10.29408/sinteza.v2i2.7662. DOI: https://doi.org/10.29408/sinteza.v2i2.7662
Pamungkas JD, Anam K, Kusrini D. Penentuan total kadar fenol dari daun kersen segar, kering dan rontok (Muntingia calabura L.) serta uji aktivitas antioksidan dengan metode DPPH. J Kim Sains Apl. 2016;19(1):15-20. doi:10.14710/jksa.19.1.15-20. DOI: https://doi.org/10.14710/jksa.19.1.15-20
Sepriyani. Aktivitas Antioksidan Ekstrak Metanol Daun Pepaya (Carica papaya L) dengan Metode 2,2-Diphenyl. [Journal information missing]. 2020. DOI: https://doi.org/10.51887/jpfi.v9i1.789
Chandra A, Wijaya RM, Noviana D. Skrining Fitokimia dan Aktivitas Antioksidan Ekstrak Metanol Daun Kemangi (Ocimum tenuiflorum L.) dengan Metode DPPH. J Pharm Sci. 2019;2(2). doi:10.36490/journal-jps.com.v2i2.20. DOI: https://doi.org/10.36490/journal-jps.com.v2i2.20
Rahayu S, Firdaus ML, Ningsih DR. Kadar Flavonoid dan Fenolik Ekstrak Daun Waru (Hibiscus tiliaceus) Serta Aktivitasnya Sebagai Antioksidan. JC-T J Kim Terap. 2022;6(1):17. doi:10.17977/um0260v6i12022p017. DOI: https://doi.org/10.17977/um0260v6i12022p017
Fahrurrozi. Uji Aktivitas Antioksidan Ekstrak Etanol Daun Petai Cina (Leucaena glauca (L.) Benth.) dengan Metode DPPH (2,2-Diphenyl-1-picrylhidrazyl). Sinteza. 2021;1(1). doi:10.29408/sinteza.v1i1.3206. DOI: https://doi.org/10.29408/sinteza.v1i1.3206
Sari IP, Handayani S, Fauziah N. Aktivitas Antioksidan Ekstrak Etanol Dan Fraksinya Dari Kulit Batang Rambutan (Nephelium lappaceum Linn) Menggunakan Metode DPPH. Window Health J Kesehat. 2020. doi:10.33368/woh.v0i0.259. DOI: https://doi.org/10.33096/woh.v3i1.564
Alam T, Ekayanti M, Permana N, Hadissabil Z. The potential antioxidant activity of ethanol extract and fraction of ciplukan (Physalis angulata) on DPPH (1,1-diphenyl-2-picrylhydrazyl). J Farm Indones. 2022;19(1):193-9. https://doi.org/10.31001/jfi.v19i1.1490. DOI: https://doi.org/10.31001/jfi.v19i1.1490
Maesaroh K, Kurnia D, Anshori J. Perbandingan metode uji aktivitas antioksidan DPPH, FRAP dan FIC terhadap asam askorbat, asam galat dan kuersetin. Chim Nat Acta. 2018;6(2):93. https://doi.org/10.24198/cna.v6.n2.19049. DOI: https://doi.org/10.24198/cna.v6.n2.19049
Puspitasari A, Anwar F, Faizah N. Aktivitas antioksidan, penetapan kadar fenolik total dan flavonoid total ekstrak etanol, etil asetat, dan n-heksan daun petai (Parkia speciosa Hassk.). J Ilm Teknosains. 2019;5(1):1-8. https://doi.org/10.26877/jitek.v5i1.3490. DOI: https://doi.org/10.26877/jitek.v5i1.3490
Luthfiyanti R, Iwansyah A, Pamungkas N, Triyono A. Penurunan mutu senyawa antioksidan dan kadar air terhadap masa simpan permen hisap ekstrak daun ciplukan (Physalis angulata Linn.). J Ris Teknol Ind. 2020;14(1):1. https://doi.org/10.26578/jrti.v14i1.5343. DOI: https://doi.org/10.26578/jrti.v14i1.5343
Setianah H, Nugraheni I, Wibowo D. Aktivitas antibakteri isolat bakteri endofit asal daun ciplukan (Physalis angulata L.) terhadap bakteri Escherichia coli dan Staphylococcus aureus. JHES (J Health Stud). 2021;5(1):50-61. https://doi.org/10.31101/jhes.1485. DOI: https://doi.org/10.31101/jhes.1485