In vitro and in silico evaluation of toxicological and anti-proliferative activity in phytochemical compounds of several solvent extracts from Zaleya pentandra L
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Abstract
Therapeutic plants and herbals are vital superior nutrient resources mainly used in diet and recognised for treating various diseases. The study's main objective is to investigate phytochemicals compounds, antioxidant and antibacterial activities on five Zaleya pentandra extract (ZPE), ethanol, hexane, acetone, ethyl acetate, and methanol for the first time. Methods: The study was conducted using the HPLC-MS, and measurements were made in three replicates. Results: The most significant extracts, revealing 13compounds, which appear higher content (417.5±0.44 μg/g and (407.5±0.04 μg/g), value of ZPE in total β-Sitosterol and dioctyl phthalate, respectively, also the total polyphenol content (TPC) was significantly (p≤0.05) higher mainly in acetone (323.06±1.74mg GAE/g), ethyl acetate (220 ± 1.00) mg GAE/g), and hexane herbal extracts (75.2±1.70) mg QE/g) with a significant difference (P≤0.05) in total flavonoid content. Discussion: All investigated bacterial strains had an exceptionally high effect against B. subtilis. A computational analysis qualified a significant drug-likeness feature, including toxicological and pharmacokinetic assessments. ZP acetone extract is an appropriate selection for creating contemporary antibacterial compounds. Conclusions: The current study offers new information on applying ZPE in novel and potentially effective therapeutic agents, its application in the food industry, and the treatment of different diseases.
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References
Mahmoud AS, Thao N, Mario A. (2016). Combination therapy using herbal drugs and antibiotics: a novel way to treat bacteria resistant to multiple drugs. Analytical Medicines Act. https://doi.org/10.4172/2153-2435.1000523. DOI: https://doi.org/10.4172/2153-2435.1000523
Aziz, M.; Ahmad, S.; Khurshid, U.; Saleem, H.; Alamri, A.; Anwar, S.; Alamri, A.S.; Chohan, T.A. (2022). Phytochemical, Pharmaceutical, and In-silicon - of Strobilanthes glutinous Nees: An unexplored source of bioactive compounds. S. Afr. J. Bot. 2022, 147, 618–627. DOI: https://doi.org/10.1016/j.sajb.2021.07.013
Fierascu, A., Fierascu, I., R.C., Baroi, A.M., and Ortan,( 2021). approximately information about aromatic and medicinal plants as alternate sources of bioactive compounds. Int. J. Mol. Sci. 22, 1521. https://doi.org/10.3390/ijms22041521. DOI: https://doi.org/10.3390/ijms22041521
Noumi, E. Snoussi, M. Alreshidi, M., Veettil, V.N., Elkahoui, S., Adnan, M., Patel, M., Kadri, A., Aouadi, K., De Feo, V., Badraoui, R., (2020). HR-LCMSTeucrium polium L. Methanolic extract: anticancer, antioxidant, and metabolite profiling based on computational and in vitro study. 9 Antioxidants 1089. https://doi.org/10.3390/9111089. DOI: https://doi.org/10.3390/antiox9111089
Fan, S., Guo, S., Suleria, H.A.R.; Qi, Y., Shi, L., Giovani, M.; Zaki, N.A.A. (2022). assessment of the antioxidant potential of the phenolic compounds screened out of the rejected avocado. 10, 1747. https://doi.org/10.3390/pr10091747. DOI: https://doi.org/10.3390/pr10091747
Felhi, S, M., Gharsallah, N., Bakari, S., Hajlaoui, H., Ncir, M., and Kadri, A. (2016). Evaluation of the fruit juice from three locations of Ecballium elaterium, freeze-dried extracts, and FT-IR analysis of the nutrients, phytochemicals, and antioxidants. N. Y.: Food Sci. Technol., 36, 646–655. https://doi.org/10.1590/1678-457X.12916. DOI: https://doi.org/10.1590/1678-457x.12916
Nyakudya, T, Tshabalala, T., Erlwanger, K.H., Dangarembizi, R., and Ndhlala, A.R., (2020). The potential benefits of using medicinal plants to treat metabolic diseases. 26,69 Molecules. https://doi.org.10.3390/molecules25112669
Alminderej, F, Bakari, S., Almundarij, Snoussi, M. (2021). A study conducted in silico on the S. aureus tyrosyl-tRNA synthetize protein revealed the antimicrobial and wound-healing potential of a novel chemotype derived from Piper cubeba L. essential oil. Plants 10, 205–229. https://doi.org/10.3390.10020205. DOI: https://doi.org/10.3390/plants10020205
Badraoui, R (2020). Lustrum sub hirsutism L. HR-LCMS phytochemical profiling, in vitro and in vivo pharmacological study as a potential source of antioxidant and anticancer bioactive molecules. Antioxidants 9, 1003. https://doi.org/10.3390/antiox9101003 DOI: https://doi.org/10.3390/antiox9101003
Vaou, N., Bezirtzoglou, E., Stavropoulou, E., Voidarou, C., Tsigalou, C., (2021). A review study on the obstacles and potential directions for future research on the antimicrobial activity of medicinal plants. Microbiology 9, 2041. 10.3390/9102041. DOI: https://doi.org/10.3390/microorganisms9102041
Mau, J.L., Chen, C.P., and Hsieh, P.C. (2001). Antimicrobial properties of corni fructus, cinnamon, and Chinese chives extracts. 183–188 in J. Agric. Food Chem. 49 (1). https://doi.org/10.1021/jf000263c DOI: https://doi.org/10.1021/jf000263c
Timmermans, K (2003). Policy dilemmas at the intersection of intellectual property rights and traditional medicine. Health Sciences & Social Science 57, 745–756. DOI: https://doi.org/10.1016/S0277-9536(02)00425-2
Saleem, H.; Mahomoodally, M.F.; Hussain, R.; Rengasamy, K.R.; Mollica, A.; Abidin, S.A.Z.; Zengin, G.; Locatelli, M.; Ahmad, I.; Khaliq, S. Food Chem. Toxicol. (2019). Pharmacological, phytochemical, and in-vivo toxicological aspects of a xero-halophyte medicinal plant: Zaleya pentandra (L.) Jeffrey. , 131, 110535 DOI: https://doi.org/10.1016/j.fct.2019.05.043
Mughal, T.A.; Aslam, F; Yousaf, Z.; Nisar, N.; Leung, P.C.; F. (2020). The breast cancer adenocarcinoma cell line was subjected to the in vitro cytotoxic activity of extracts from Zaleya pentandra L. JPMA; MCF-7; 2019.
Seifu, T., Asres, K., and. Gebre-Mariam, T. (2006). Ethnobotanical and ethno-pharmaceutical studies of the medicinal plants in the Chifra District, Afar Region, Northeastern Ethiopia. Journal of Ethiopian Pharmacy 24, 41–58. DOI: https://doi.org/10.4314/epj.v24i1.35097
Qasim, M., Khan, M.A., and Gulzar, S. (2011). Halophytes as medicinal flora, Denizli, Turkey: NAM Conference.
Burkill, H. (1985). The West Tropical Africa's Useful Plants at the Royal Botanic Gardens in Kew. Distributed by: Royal Botanic Gardens 9781900347136, 190034713X; digitised July 15, 2011.
Mahmood, A. Shaheen, H. Qureshi, Y. Sangi, and S.A. Gilani. (2011). An investigation into the traditional medical applications of plants in Pakistan's Jammu and Kashmir's Bhimber district. Medicinal Plant Research, Volume 5, Issues 2348–2360.
Felhi, S., Gharsallah, N., Mnafgui, K., Daoud, A., Hajlaoui, H., and Kadri, A. (2017). Ecballium elaterium seeds and peels fruits: effects of solvent extraction on phytochemical constituent profiles, antioxidant and antimicrobial activities, and functional group analysis. 37, 483–492; Food Sci. Technol. https://doi.org/10.1590/1678-457X.23516 DOI: https://doi.org/10.1590/1678-457x.23516
Sharma, Shailendra; Bhandari Anil Chaudhary Deepak (2014). Analysis of phytochemical and pharmacognostic properties of the leaf part of the medicinal herb Zaleya Govinda. Journal of Drug Development and Research, January–March 2014, 6 (1): 153–159.
Bakari, S., Hajlaoui, H., Daoud, A., Mighri, H., Ross-Garcia, J.M., Gharsallah, N., and Kadri, A. (2018). The hydroalcoholic extracts of Exordium glaucophyllum leaves and flowers collected from the Tunisian Sahara were subjected to LC-MS analysis to determine their phytochemical, antioxidant, and antimicrobial potential. Food Sci. Biotechnology. 38: 310–317. https://doi.org/10.1590/fst.04517. DOI: https://doi.org/10.1590/fst.04517
Miliauskas, G. Venskutonis, P.R and T.A. van Beek, (2004). screening of some aromatic and medicinal plant extracts for their capacity to scavenge free radicals. Food Chem. 85, 231–237. https://doi.org/10.1016/j.foodchem.2003.05.007 DOI: https://doi.org/10.1016/j.foodchem.2003.05.007
Hajlaoui, H., Nieto, G., Ros, G., Chaaibia, M., Arraouadi, S., Mighri, H., Ros, G., and Kadri, A., (2019). Oudneya Africana L. leaf extracts' phytochemical components and antioxidant activity: an assessment of their effects on the oxidation of proteins and fatty acids in beef burgers stored in a refrigerator. 8, 442; antioxidants. https://doi.org/10.3390/antiox8100442. DOI: https://doi.org/10.3390/antiox8100442
Bakari, S., Felhi, S., Daoud, A., Gharsallah, N., and Kadri, A. (2017). The present study examines the proximate analysis, mineral composition, phytochemical contents, antioxidant and antimicrobial activities, and GC-MS investigation of different solvent extracts of cactus. 27, 286–293. Food Sci. Technol. 10.1590/1678-457X.20116. DOI: https://doi.org/10.1590/1678-457x.20116
Sembiring, E.N; Alya, B.; Sauriasari, R.;(2018). Biochemical analysis, complete flavonoid and total phenolic content, and antioxidant capacity of various Caesalpinia bonduc (L.) Roxb. Pharmacogn. J. sections 2018, 10, 123–127. DOI: https://doi.org/10.5530/pj.2018.1.22
Aouadi, K., Ghannay, S., Arraouadi, S., Hajlaoui, H., Snoussi, M., and Kadri, A., (2021). Echinus is humble and deaf. Extracts' phytochemical profile using HPLC/MS and their antioxidant properties: ADMET prediction and computational analysis focusing on the human peroxiredoxin five receptors. 21,65 in Agronomy 11. Agronomy11112165, https://doi.org/10.3390 DOI: https://doi.org/10.3390/agronomy11112165
Daoud, A., Malika, D., Bakari, S., Hfaiedh, N., Mnafgui, K., Kadri, A., Gharsallah, N., and Malika, D., (2019). Evaluation of different Date Palm Pollen (DPP) extracts' polyphenol content, antioxidant, and antibacterial qualities from two Tunisian cultivars. 10.1016/j.arabjc.2015.07.014; Arab. J. Chem. 12, 3075–3086. DOI: https://doi.org/10.1016/j.arabjc.2015.07.014
Othman, I.M.M., Anouar, E.H., Aouadi, K., Snoussi, M., Kadri, A. Gad-Elkareem, M.A.M. (2021). Prospective inhibitors of tyrosyl-tRNA synthetase and peroxiredoxin-5: design, synthesis, molecular docking, and structure-activity relationship (SAR) analysis of novel substituted pyrazolones and dipyrazolotriazines. 109. https://doi.org/10.1016/j.bioorg.2021.10470. DOI: https://doi.org/10.1016/j.bioorg.2021.104704
Almuhanna, Y., Alrouji, M., Kuriri, F.A., Alissa, M., Alsuwat, M.A., Asad, M., Joseph, B., Alqasmi, M.H., AlSudais, H., Almuhanna (2023). Influence of Achillea fragrantissima extraction on MRSA and Pseudomonas aeruginosa excision site biofilms in diabetic rodents. J. Mol. Sci. Int. 24, 9774. https://doi.org/10.3390/ijms24119774. DOI: https://doi.org/10.3390/ijms24119774
Ghannay, S., Bakari, S., Msaddek, M., Vidal, S., Kadri, A., and Aouadi, K. (2020). New enantiopure isoxazolidine derivatives: design, synthesis, molecular characteristics, and in vitro antioxidant and antibacterial potential. Arab. J. Chem. 13, 2121–2131. https://doi.org/10.1016/j.arabjc.2018.03.013 DOI: https://doi.org/10.1016/j.arabjc.2018.03.013
Basit, A.; Ahmad, S.; Khan, M.A.; Rao, H.; Ahmad, I.; Shahzad, M.N.; Ghalloo, B.A.; Sherif, A.E.; Aati, H.Y.; Ovatlarnporn, C (. 2022). Recent mechanistic insights into Justicia vahlii Roth include antioxidant-based anti-inflammatory, in-vivo, in-silico toxicological, and enzyme inhibition assessment using UPLC-Q-TOF-MS and GC-MS based metabolomics, 15, 104135; Arab. J. Chem. DOI: https://doi.org/10.1016/j.arabjc.2022.104135
Rodrigues, M.J.; Custódio, L.; Mecha, D.; Zengin, G.; Cziáky, Z.; Sotkó, G.; Pereira, C.G. (2022). Nutritional and Photo-Therapeutic Value of the Halophyte Caladium Marcus L. (Pohl.): A Special Focus on Seeds. Plants, 11, 2910. DOI: https://doi.org/10.3390/plants11212910
Wang, W.; Chen, Y.; Zhang, M.; Yang, L.; Wu, X.; Feng, W.; Wu, X.; Mao, G.; Zhao, T.; Wang, W. (2020). Effects on developing mice's brains of subchronic exposure to individual and combination doses of lead, arsenic, and dioctyl phthalate (DOP)., 27,9247–9260; Environ. Sci. Pollut. Res. DOI: https://doi.org/10.1007/s11356-019-06823-2
Kamireddy, K.; Rajini, P.; Giridhar, P.; Chinnu, S.; Priyanka, P. (2018). Caenorhabditis elegans 6-OHDA-induced neurotoxicity was mitigated by the neuroprotective effects of Decalepis Hamiltonian aqueous root extract and purified 2-hydroxy-4-methoxy benzaldehyde., 105, 997–1005; Biomed. Pharmacotherapy. DOI: https://doi.org/10.1016/j.biopha.2018.06.002
Nipun, T.S., Khatib, A., Ibrahim, Z., Ahmed, Q.U., Redzwan, I.E. Saiman, M.Z.; Fairuza, R.; Widyaningsih, T.D.; AlAjmi, M.F. (2021). GC-MS-and NMR-Based Metabolomics and Molecular Docking Reveal the Potential Alpha-Glucosidase Inhibitors from Psychotic Malayans Pharmaceuticals, 14, 978; Jack departs. DOI: https://doi.org/10.3390/ph14100978
Prager N, French N, Bickett K, and Marcovici G (2002). An open, double-masked, placebo-controlled study evaluated the efficacy of botanically derived 5-alpha-reductase inhibitors in managing androgenetic alopecia. 2002;8(2):143-152.12006122; J Altern Complement Med. DOI: https://doi.org/10.1089/acm.2002.8.143
Afia Shahid, Huma R., Hanan Y., and Kashif ur R. K. Aati Asmaa E. S., Sameh A. Korma, Tuba E., Muhammad U., Abdul Muee, Abdul B., and Duraiz A. K. (2023). In-vitro assays and in-silico molecular docking, phytochemical profiling of the ethanolic extract of Zaleya p.L. and its biological activities were investigated. Appl. Sci., 13, 584. https://doi.org/10.3390/app13010584. DOI: https://doi.org/10.3390/app13010584
Fair RJ, and Tor Y. Antibiotics and bacterial resistance in the 21st century (2014). Prospect Medicine Chem.; 6:25-64. https://doi.org/10.4137/PMC.S1445. DOI: https://doi.org/10.4137/PMC.S14459