Main Article Content

Mohamed O. Elsamani

Page: 100-114


Therapeutic plants and herbals are vital superior nutrient resources mainly used in diet and recognised for treating various diseases. The study's main objective is to investigate phytochemicals compounds, antioxidant and antibacterial activities on five Zaleya pentandra extract (ZPE), ethanol, hexane, acetone, ethyl acetate, and methanol for the first time.  Methods: The study was conducted using the HPLC-MS, and measurements were made in three replicates.  Results: The most significant extracts, revealing 13compounds, which appear higher content (417.5±0.44 μg/g and (407.5±0.04 μg/g), value of ZPE in total β-Sitosterol and dioctyl phthalate, respectively, also the total polyphenol content (TPC) was significantly (p≤0.05) higher mainly in acetone (323.06±1.74mg GAE/g), ethyl acetate (220 ± 1.00) mg GAE/g), and hexane herbal extracts (75.2±1.70) mg QE/g) with a significant difference (P≤0.05) in total flavonoid content. Discussion: All investigated bacterial strains had an exceptionally high effect against B. subtilis. A computational analysis qualified a significant drug-likeness feature, including toxicological and pharmacokinetic assessments. ZP acetone extract is an appropriate selection for creating contemporary antibacterial compounds. Conclusions: The current study offers new information on applying ZPE in novel and potentially effective therapeutic agents, its application in the food industry, and the treatment of different diseases.


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Elsamani, M. . O. (2024). In vitro and in silico evaluation of toxicological and anti-proliferative activity in phytochemical compounds of several solvent extracts from Zaleya pentandra L. Journal of Pharmaceutical and Sciences, 7(1), 100–114.
Original Articles


Mahmoud AS, Thao N, Mario A. (2016). Combination therapy using herbal drugs and antibiotics: a novel way to treat bacteria resistant to multiple drugs. Analytical Medicines Act. DOI:

Aziz, M.; Ahmad, S.; Khurshid, U.; Saleem, H.; Alamri, A.; Anwar, S.; Alamri, A.S.; Chohan, T.A. (2022). Phytochemical, Pharmaceutical, and In-silicon - of Strobilanthes glutinous Nees: An unexplored source of bioactive compounds. S. Afr. J. Bot. 2022, 147, 618–627. DOI:

Fierascu, A., Fierascu, I., R.C., Baroi, A.M., and Ortan,( 2021). approximately information about aromatic and medicinal plants as alternate sources of bioactive compounds. Int. J. Mol. Sci. 22, 1521. DOI:

Noumi, E. Snoussi, M. Alreshidi, M., Veettil, V.N., Elkahoui, S., Adnan, M., Patel, M., Kadri, A., Aouadi, K., De Feo, V., Badraoui, R., (2020). HR-LCMSTeucrium polium L. Methanolic extract: anticancer, antioxidant, and metabolite profiling based on computational and in vitro study. 9 Antioxidants 1089. DOI:

Fan, S., Guo, S., Suleria, H.A.R.; Qi, Y., Shi, L., Giovani, M.; Zaki, N.A.A. (2022). assessment of the antioxidant potential of the phenolic compounds screened out of the rejected avocado. 10, 1747. DOI:

Felhi, S, M., Gharsallah, N., Bakari, S., Hajlaoui, H., Ncir, M., and Kadri, A. (2016). Evaluation of the fruit juice from three locations of Ecballium elaterium, freeze-dried extracts, and FT-IR analysis of the nutrients, phytochemicals, and antioxidants. N. Y.: Food Sci. Technol., 36, 646–655. DOI:

Nyakudya, T, Tshabalala, T., Erlwanger, K.H., Dangarembizi, R., and Ndhlala, A.R., (2020). The potential benefits of using medicinal plants to treat metabolic diseases. 26,69 Molecules.

Alminderej, F, Bakari, S., Almundarij, Snoussi, M. (2021). A study conducted in silico on the S. aureus tyrosyl-tRNA synthetize protein revealed the antimicrobial and wound-healing potential of a novel chemotype derived from Piper cubeba L. essential oil. Plants 10, 205–229. DOI:

Badraoui, R (2020). Lustrum sub hirsutism L. HR-LCMS phytochemical profiling, in vitro and in vivo pharmacological study as a potential source of antioxidant and anticancer bioactive molecules. Antioxidants 9, 1003. DOI:

Vaou, N., Bezirtzoglou, E., Stavropoulou, E., Voidarou, C., Tsigalou, C., (2021). A review study on the obstacles and potential directions for future research on the antimicrobial activity of medicinal plants. Microbiology 9, 2041. 10.3390/9102041. DOI:

Mau, J.L., Chen, C.P., and Hsieh, P.C. (2001). Antimicrobial properties of corni fructus, cinnamon, and Chinese chives extracts. 183–188 in J. Agric. Food Chem. 49 (1). DOI:

Timmermans, K (2003). Policy dilemmas at the intersection of intellectual property rights and traditional medicine. Health Sciences & Social Science 57, 745–756. DOI:

Saleem, H.; Mahomoodally, M.F.; Hussain, R.; Rengasamy, K.R.; Mollica, A.; Abidin, S.A.Z.; Zengin, G.; Locatelli, M.; Ahmad, I.; Khaliq, S. Food Chem. Toxicol. (2019). Pharmacological, phytochemical, and in-vivo toxicological aspects of a xero-halophyte medicinal plant: Zaleya pentandra (L.) Jeffrey. , 131, 110535 DOI:

Mughal, T.A.; Aslam, F; Yousaf, Z.; Nisar, N.; Leung, P.C.; F. (2020). The breast cancer adenocarcinoma cell line was subjected to the in vitro cytotoxic activity of extracts from Zaleya pentandra L. JPMA; MCF-7; 2019.

Seifu, T., Asres, K., and. Gebre-Mariam, T. (2006). Ethnobotanical and ethno-pharmaceutical studies of the medicinal plants in the Chifra District, Afar Region, Northeastern Ethiopia. Journal of Ethiopian Pharmacy 24, 41–58. DOI:

Qasim, M., Khan, M.A., and Gulzar, S. (2011). Halophytes as medicinal flora, Denizli, Turkey: NAM Conference.

Burkill, H. (1985). The West Tropical Africa's Useful Plants at the Royal Botanic Gardens in Kew. Distributed by: Royal Botanic Gardens 9781900347136, 190034713X; digitised July 15, 2011.

Mahmood, A. Shaheen, H. Qureshi, Y. Sangi, and S.A. Gilani. (2011). An investigation into the traditional medical applications of plants in Pakistan's Jammu and Kashmir's Bhimber district. Medicinal Plant Research, Volume 5, Issues 2348–2360.

Felhi, S., Gharsallah, N., Mnafgui, K., Daoud, A., Hajlaoui, H., and Kadri, A. (2017). Ecballium elaterium seeds and peels fruits: effects of solvent extraction on phytochemical constituent profiles, antioxidant and antimicrobial activities, and functional group analysis. 37, 483–492; Food Sci. Technol. DOI:

Sharma, Shailendra; Bhandari Anil Chaudhary Deepak (2014). Analysis of phytochemical and pharmacognostic properties of the leaf part of the medicinal herb Zaleya Govinda. Journal of Drug Development and Research, January–March 2014, 6 (1): 153–159.

Bakari, S., Hajlaoui, H., Daoud, A., Mighri, H., Ross-Garcia, J.M., Gharsallah, N., and Kadri, A. (2018). The hydroalcoholic extracts of Exordium glaucophyllum leaves and flowers collected from the Tunisian Sahara were subjected to LC-MS analysis to determine their phytochemical, antioxidant, and antimicrobial potential. Food Sci. Biotechnology. 38: 310–317. DOI:

Miliauskas, G. Venskutonis, P.R and T.A. van Beek, (2004). screening of some aromatic and medicinal plant extracts for their capacity to scavenge free radicals. Food Chem. 85, 231–237. DOI:

Hajlaoui, H., Nieto, G., Ros, G., Chaaibia, M., Arraouadi, S., Mighri, H., Ros, G., and Kadri, A., (2019). Oudneya Africana L. leaf extracts' phytochemical components and antioxidant activity: an assessment of their effects on the oxidation of proteins and fatty acids in beef burgers stored in a refrigerator. 8, 442; antioxidants. DOI:

Bakari, S., Felhi, S., Daoud, A., Gharsallah, N., and Kadri, A. (2017). The present study examines the proximate analysis, mineral composition, phytochemical contents, antioxidant and antimicrobial activities, and GC-MS investigation of different solvent extracts of cactus. 27, 286–293. Food Sci. Technol. 10.1590/1678-457X.20116. DOI:

Sembiring, E.N; Alya, B.; Sauriasari, R.;(2018). Biochemical analysis, complete flavonoid and total phenolic content, and antioxidant capacity of various Caesalpinia bonduc (L.) Roxb. Pharmacogn. J. sections 2018, 10, 123–127. DOI:

Aouadi, K., Ghannay, S., Arraouadi, S., Hajlaoui, H., Snoussi, M., and Kadri, A., (2021). Echinus is humble and deaf. Extracts' phytochemical profile using HPLC/MS and their antioxidant properties: ADMET prediction and computational analysis focusing on the human peroxiredoxin five receptors. 21,65 in Agronomy 11. Agronomy11112165, DOI:

Daoud, A., Malika, D., Bakari, S., Hfaiedh, N., Mnafgui, K., Kadri, A., Gharsallah, N., and Malika, D., (2019). Evaluation of different Date Palm Pollen (DPP) extracts' polyphenol content, antioxidant, and antibacterial qualities from two Tunisian cultivars. 10.1016/j.arabjc.2015.07.014; Arab. J. Chem. 12, 3075–3086. DOI:

Othman, I.M.M., Anouar, E.H., Aouadi, K., Snoussi, M., Kadri, A. Gad-Elkareem, M.A.M. (2021). Prospective inhibitors of tyrosyl-tRNA synthetase and peroxiredoxin-5: design, synthesis, molecular docking, and structure-activity relationship (SAR) analysis of novel substituted pyrazolones and dipyrazolotriazines. 109. DOI:

Almuhanna, Y., Alrouji, M., Kuriri, F.A., Alissa, M., Alsuwat, M.A., Asad, M., Joseph, B., Alqasmi, M.H., AlSudais, H., Almuhanna (2023). Influence of Achillea fragrantissima extraction on MRSA and Pseudomonas aeruginosa excision site biofilms in diabetic rodents. J. Mol. Sci. Int. 24, 9774. DOI:

Ghannay, S., Bakari, S., Msaddek, M., Vidal, S., Kadri, A., and Aouadi, K. (2020). New enantiopure isoxazolidine derivatives: design, synthesis, molecular characteristics, and in vitro antioxidant and antibacterial potential. Arab. J. Chem. 13, 2121–2131. DOI:

Basit, A.; Ahmad, S.; Khan, M.A.; Rao, H.; Ahmad, I.; Shahzad, M.N.; Ghalloo, B.A.; Sherif, A.E.; Aati, H.Y.; Ovatlarnporn, C (. 2022). Recent mechanistic insights into Justicia vahlii Roth include antioxidant-based anti-inflammatory, in-vivo, in-silico toxicological, and enzyme inhibition assessment using UPLC-Q-TOF-MS and GC-MS based metabolomics, 15, 104135; Arab. J. Chem. DOI:

Rodrigues, M.J.; Custódio, L.; Mecha, D.; Zengin, G.; Cziáky, Z.; Sotkó, G.; Pereira, C.G. (2022). Nutritional and Photo-Therapeutic Value of the Halophyte Caladium Marcus L. (Pohl.): A Special Focus on Seeds. Plants, 11, 2910. DOI:

Wang, W.; Chen, Y.; Zhang, M.; Yang, L.; Wu, X.; Feng, W.; Wu, X.; Mao, G.; Zhao, T.; Wang, W. (2020). Effects on developing mice's brains of subchronic exposure to individual and combination doses of lead, arsenic, and dioctyl phthalate (DOP)., 27,9247–9260; Environ. Sci. Pollut. Res. DOI:

Kamireddy, K.; Rajini, P.; Giridhar, P.; Chinnu, S.; Priyanka, P. (2018). Caenorhabditis elegans 6-OHDA-induced neurotoxicity was mitigated by the neuroprotective effects of Decalepis Hamiltonian aqueous root extract and purified 2-hydroxy-4-methoxy benzaldehyde., 105, 997–1005; Biomed. Pharmacotherapy. DOI:

Nipun, T.S., Khatib, A., Ibrahim, Z., Ahmed, Q.U., Redzwan, I.E. Saiman, M.Z.; Fairuza, R.; Widyaningsih, T.D.; AlAjmi, M.F. (2021). GC-MS-and NMR-Based Metabolomics and Molecular Docking Reveal the Potential Alpha-Glucosidase Inhibitors from Psychotic Malayans Pharmaceuticals, 14, 978; Jack departs. DOI:

Prager N, French N, Bickett K, and Marcovici G (2002). An open, double-masked, placebo-controlled study evaluated the efficacy of botanically derived 5-alpha-reductase inhibitors in managing androgenetic alopecia. 2002;8(2):143-152.12006122; J Altern Complement Med. DOI:

Afia Shahid, Huma R., Hanan Y., and Kashif ur R. K. Aati Asmaa E. S., Sameh A. Korma, Tuba E., Muhammad U., Abdul Muee, Abdul B., and Duraiz A. K. (2023). In-vitro assays and in-silico molecular docking, phytochemical profiling of the ethanolic extract of Zaleya p.L. and its biological activities were investigated. Appl. Sci., 13, 584. DOI:

Fair RJ, and Tor Y. Antibiotics and bacterial resistance in the 21st century (2014). Prospect Medicine Chem.; 6:25-64. DOI: