Antioxidant Activity Test of Kelakai Leaf Kombucha (Stenochlaena palustris (Burm.F) Bedd) Using The DPPH Method
Article Sidebar
Main Article Content
Page: 588-597
Abstract
Degenerative diseases are often caused by exposure to free radicals, and antioxidants play an important role in maintaining body stability by counteracting them. Kelakai (Stenochlaena palustris), a typical plant of Kalimantan, contains bioactive compounds such as phenolics and flavonoids, making it a potential source of natural antioxidants. This study aims to determine the results of antioxidant activity tests on kelakai leaf kombucha. Kombucha was chosen because its antioxidant activity is influenced by the phenolic content formed during fermentation, potentially becoming an antioxidant-rich probiotic drink. The antioxidant activity test of kelakai leaf kombucha used the DPPH (2,2-diphenyl-1-picrylhydrazyl) method. The results of determining the maximum absorption wavelength of 0.1 mM DPPH were at 515 nm with the highest absorption of 0.602. The antioxidant activity test using quercetin as a comparison resulted in an IC₅₀ value of 3.0892 ppm. This quercetin IC₅₀ value is included in the very strong category (<50 ppm). Meanwhile, the antioxidant activity test on kelakai leaf kombucha yielded an IC₅₀ value of 0.5845 ppm. This value also indicates that kelakai leaf kombucha has very strong antioxidant activity. Increasing sample concentration is directly proportional to the increase in antioxidant activity, as indicated by a decrease in the absorption value. Therefore, the development of kelakai leaf kombucha and testing its antioxidant activity are expected to produce innovative functional food products.
Downloads
Article Details
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
References
Widiasriani, I. A. P., Udayani, N. N. W., Putri Triansyah, G. A., Mahita Kumari Dewi, N. P. E., Eva Wulandari, N. L. W., & Sri Prabandari, A. A. S. Artikel Review: Peran Antioksidan Flavonoid dalam Menghambat Radikal Bebas. 2024. Journal Syifa Sciences and Clinical Research, 6(2). 188-197. https://doi.org/10.37311/jsscr.v6i2.27055 DOI: https://doi.org/10.37311/jsscr.v6i2.27055
Pratama, A. N., & Busman, H. Potential of Soybean Antioxidant (Glycine Max L) on Capturing Free Radicals. 2020. Jurnal Ilmu Kesehatan Sandi Husada.11(1), 497–504. https://doi.org/10.35816/jiskh.v10i2.333 DOI: https://doi.org/10.35816/jiskh.v11i1.333
Mareta, C. A . Efektifitas Pegagan (Centella Asiatica) Sebagai Antioksidan. 2020. Jurnal medika hutama. 01(02), 390-394.
Adawiyah, R., & Rizki, M. I. Aktivitas Antioksidan Ekstrak Etanol Akar Kalakai (Stenochlaena palustris Bedd) Asal Kalimantan Tengah. 2018. Jurnal Pharmascience, 05(01), 71–77. DOI: https://doi.org/10.20527/jps.v5i1.5788
Savitri, S. A., Hakim, A. R., & Saputri, R. Aktivitas Antioksidan Dari Infusa Kelakai (Stenochlaena palustris (Burm.F) Bedd). 2021. In Journal of Pharmaceutical Care and Sciences. 2(1).133-138. DOI: https://doi.org/10.33859/jpcs.v2i1.69
Sihite, N. W., & Hutasoit, M. S. Potensi Bahan Pangan Lokal Indonesia Sebagai Pangan Fungsional Dan Manfaatnya Bagi Kesehatan: Review. 2023. Jurnal Riset Gizi. 11(2). 133–138. https://doi.org/10.31983/jrg.v11i2.9488 DOI: https://doi.org/10.31983/jrg.v11i2.9488
Julizan, N., Maemunah, S., Dwiyanti, D., & Al Anshori, J. Validasi Penentuan Aktifitas Antioksidan Dengan Metode DPPH. 2019. Kandaga. 1(1). 41-48. DOI: https://doi.org/10.24198/kandaga.v1i1.21473
Sopiah, B., Muliasari, H., & Yuanita, E. Skrining Fitokimia dan Potensi Aktivitas Antioksidan Ekstrak Etanol Daun Hijau dan Daun Merah Kastuba. 2019. Jurnal Ilmu Kefarmasian Indonesia. 17(1), 27–33. DOI: https://doi.org/10.35814/jifi.v17i1.698
Lady, D., Handoyo, Y., Eko, M., Program, P., Farmasi, S., & Kesehatan, I. Pengaruh Variasi Suhu Pengeringan Terhadap Pembuatan Simplisia Daun Mimba (Azadirachta Indica). 2020. In Jurnal Farmasi Tinctura. 1(2). 45-54. DOI: https://doi.org/10.35316/tinctura.v1i2.988
Riansyah, K., Hakim, A., & Hidayati, A. R. Teh herbal terstandar simplisia bunga gemitir (Tagetes erecta Linn.) sebagai kandidat antioksidan baru. 2023. Sasambo Journal of Pharmacy, 4(1), 45–52. https://doi.org/10.29303/sjp.v4i1.194 DOI: https://doi.org/10.29303/sjp.v4i1.194
Maisyah, A., Jafar, G., & Khiong, T. K. Penentuan Aktivitas Antioksidan dari Tiga Jenis Simplisia Jahe (Gajah, Emprit, Merah) untuk Pengobatan Tradisional Chinese Medicine (TCM). 2024. Majalah Farmasetika, 9(3), 293–300. https://doi.org/10.24198/mfarmasetika.v9i3.54494 DOI: https://doi.org/10.24198/mfarmasetika.v9i3.54494
Wahyuningtias, D. S., Fitriana, A. S., & Nawangsari, D. Pengaruh Suhu dan Lamaaktu FermentasiTerhadap Sifat Organoleptik dan Aktivitas Antioksidan Teh Kombucha Bunga Telang (Clitoria ternatea L.). 2023. Pharmacy Genius. 02(03), 198–207. DOI: https://doi.org/10.56359/pharmgen.v2i3.297
Muthia, R., Hidayatullah, M., & Hidayati, R. Phytochemical Screening and Antioxidant Activity of Ethanolic Extract of Cawat Hanoman Stem (Bauhinia aculeata L.) using DPPH Method. 2020. Borneo Journal of Pharmacy. 3(1). 15–21. https://doi.org/10.33084/bjop.v3i1.1245 DOI: https://doi.org/10.33084/bjop.v3i1.1245
Nathania, E. K., Maarisit, W., Potalangi, N. O., & Tapehe, Y. Uji Aktivitas Antioksidan Ekstrak Etanol Daun Kecubung Hutan (Brugmansia Suaveolens Bercht. & amp; J. Presl) Dengan Menggunakan Metode DPPH (1,1-diphenyl-2-picrylhydrazyl). 2020. Biofarmasetikal Tropis. 3(2).40–47. https://doi.org/10.55724/j.biofar.trop.v3i2.283 DOI: https://doi.org/10.55724/j.biofar.trop.v3i2.283
Juwita, D. A., Muchtar, H., & Putri, R. K. Uji Antioksidan Ekstrak Etanol Kulit Buah dan Daging Buah Menteng (Baccaurea racemosa (Blume) Mull. Arg.) dengan Metode DPPH (2,2 diphenyl-1-picrylhydrazyl). 2020. SCIENTA Jurnal Farmasi Dan Kesehatan. 10(1). 56–62. DOI: https://doi.org/10.36434/scientia.v10i1.214
Sami, F. J., Nur, S., Ramli, N., Sutrisno, B., Tinggi, S., Farmasi Makassar, I., Farmasi, A., & Makassar, K. Uji Aktivitas Antioksidan Daun Kersen (Muntingia Calabura L.) Dengan Metode Dpph (1,1-Difenil-2-Pikrilhidrazil) Dan Frap (Ferric Reducing Antioxidan Power). 2017. As-Syifaa. 09(02). 106–111. DOI: https://doi.org/10.33096/ja.v9i2.258
Oktaria, D., & Marpaung, M. P. Penetapan Kadar Flavonoid Total Dan Aktivitas Antioksidan Ekstrak Akar Nipah (Nypa Fruticans Wurmb) Dengan Metode Spektrofotometri Uv-Vis. 2023. In Lantanida Journal. 11(1). 36-50. DOI: https://doi.org/10.22373/lj.v11i1.16087
Muthia, R., Saputri, R., Verawati, A. 2019. Uji Aktivitas Antioksidan Ekstrak Etanol Kulit Buah Mundar (Garcinia forbesii King.) Menggunakan Metode DPPH (2,2-Diphenyl-1-Picrylhydrazil). 2019. Jurnal Pharmascience. 06(01). 74–82. DOI: https://doi.org/10.20527/jps.v6i1.6079
Aini, A. N., Aisha, A., Ginting, D., Aminulloh, G., Putri, O., & Rahmandah, S. Karakteristik dan Aktivitas Antioksidan Permen Jeli Kombucha Daun Sirsak (Annona muricata Linn.).2024. Warta Akab. 48(1), 68-73. DOI: https://doi.org/10.55075/wa.v48i1.215
Susiani, E. F., Saputri, R., Wati, H., Mahmudah, F., & Vebruati. Aktivitas Antioksidan Ekstrak Metanol Daun Tandui (Mangifera rufocostata K.) Menggunakan Metode DPPH. 2024. Borneo Journal of Pharmascientech. 08(01). 90-97. https://doi.org/10.51817/bjp.v7i1.477 DOI: https://doi.org/10.51817/bjp.v8i1.477
Ariani, N., Musiam, S., Niah, R., & Dwi, R. F. Pengaruh Metode Pengeringan Terhadap Kadar Flavonoid Ekstrak Etanolik Kulit Buah Alpukat (Persea americana Mill.) dengan Spektrofotometri UV-Vis. 2022. Jurnal Pharmascience, 9(1), 40–47. DOI: https://doi.org/10.20527/jps.v9i1.10864
Zulfa, A. N., Hidayah, H., Nurjanah, A., Septanti, R., & Nadeak, Z. T. Literature Review Article : Perbandingan Kadar Antioksidan Pada Tumbuhan Jamblang Dengan Metode DPPH, FRAP, dan ABTS. 2024. Innovative: Journal Of Social Science Research, 4(1), 3359–3373.
Oktari, I. ayu, Wijayanti, K., & Anwar, M. C. Skrining Senyawa Fitokimia Daun Kelakai (Stecnochlaena palustris) Asal Kalimantan Tengah Sebagai Alternatif Penyembuhan Luka. 2025. Jurnal Kesehatan, 18(1), 73–78. https://doi.org/10.32763/k1z88f50 DOI: https://doi.org/10.32763/k1z88f50
Nasution, I. W., & Nasution, N. H. Peluang Minuman Teh Kombucha Dan Potensinya Sebagai Minuman Kesehatan Pencegah Dan Penyembuh Aneka Penyakit. 2022. Journal Of Comprehensive Science, 1(1). 9-16. https://doi.org/https://doi.org/10.36418/jcs.v1i1.2 DOI: https://doi.org/10.59188/jcs.v1i1.2
Putri, K. A., & Sabrina. Potensi Kombucha Sebagai Minuman Probiotik dan Sumber Antioksidan. 2024. Jurnal Ilmiah Wahana Pendidikan, 10(5), 37–44.
Faizah, Khairunnisa, A., Latifasari, N., & Kurniawati, A. D. Kombucha Dan Sifat Fungsionalnya: Studi Pustaka. 2024. Jurnal Sains dan Teknologi Pangan. (9)5. 7729-7741. https://doi.org/10.63071/42pr8595 DOI: https://doi.org/10.63071/42pr8595
Sinala, S., & Dewi, S. T. R. Penentuan Aktivitas Antioksidan Secara In Vitro Dari Ekstrak Etanol Propolis Dengan Metode DPPH (1,1-Difenil-2-Pikrilhidrazil). 2019. Media Farmasi, 15(1), 91. https://doi.org/10.32382/mf.v15i1.820 DOI: https://doi.org/10.32382/mf.v15i1.820
Cahyaningsih, E., Era Sandhi, P. K., & Santoso, P. Skrining Fitokimia Dan Uji Aktivitas Antioksidan Ekstrak Etanol Bunga Telang (Clitoria Ternatea L.) Dengan Metode Spektrofotometri Uv-Vis. 2019. In Ilmiah Medicamento. 5(1). 51-57. DOI: https://doi.org/10.36733/medicamento.v5i1.851