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Ramadhan Idam Choliq
Prisma Trida Hardani
Amanda Safithri Sinulingga

Page: 571-579

Abstract

Curcuma rhizome contains antioxidants, where the compound that acts as an antioxidant is curcumin. Temulawak rhizome extract tablets as a supporting supplement for the treatment process of diseases such as liver function disorders, ulcers, coughs, diarrhea, and constipation. This study was conducted to obtain a valid method in determining curcumin levels in temulawak rhizome extract tablet preparations by HPLC. The stages carried out in this study were the preparation of the mobile phase, standard preparation, HPLC optimization, sample preparation, and method validation. Validation consists of several parameters, namely linearity, LOD and LOQ, selectivity, precision, and accuracy. In the curcumin standard all parameters have met the requirements, linearity (r = 0.9975), LOD (6.5420 mg/L) and LOQ (21.8067 mg/L), selectivity (Rs = 1.7139). In brand A curcumin tablets, precision (RSD < 5.7%), while for accuracy (recovery 90-107%). The selected method can be used for the determination of curcumin content in temulawak rhizome extract tablet preparations.

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How to Cite
Choliq, R. I., Hardani , P. T., & Sinulingga, A. S. (2024). Method Validation of Curcumin Content Determination in Curcuma Rhizome Extract Tablets (Curcuma xanthorrhiza Roxb.) by HPLC. Journal of Pharmaceutical and Sciences, 7(4), 571–579. https://doi.org/10.36490/journal-jps.com.v7i4.565
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Original Articles

References

Nanda EV, Yopi Y, Pratiwi Y. Validasi Dan Penetapan Kadar Kurkumin Pada Jamu Gendong Kunyit Asam Dengan Metode Kromatografi Cair Kinerja Tinggi. Sainstech Farma: Jurnal Ilmu Kefarmasian 2021;14:12–8. https://doi.org/10.37277/sfj.v14i1.932.

Widyastuti I, Luthfah HZ, Hartono YI, Islamadina R, Can AT, Rohman A. Antioxidant Activity of Temulawak (Curcuma xanthorrhiza Roxb.) and its Classification with Chemometrics. Indonesian Journal of Chemometrics and Pharmaceutical Analysis 2021;1:28–41. https://doi.org/10.22146/ijcpa.507. DOI: https://doi.org/10.22146/ijcpa.507

Kusbiantoro D, Purwaningrum Y. Pemanfaatan kandungan metabolit sekunder pada tanaman kunyit dalam mendukung peningkatan pendapatan masyarakat. Jurnal Kultivasi 2018;17. https://doi.org/10.24198/kultivasi.v17i1.15669. DOI: https://doi.org/10.24198/kultivasi.v17i1.15669

Susanto, Ermin Katrin Winarno. Determination of Curcumin from Curcuma Plants after Gamma Irradiation, Indonesia: Center for Isotopes and Radiation Application, National Nuclear Energy Agency; 2018, p. 247.

Hanwar D, Handayani VR, Suhendi A. Validasi Metode HPLC untuk Analisis Kurkumin pada Ekstrak Rimpang Temulawak (Curcuma xanthorrhiza Roxb.). Prosiding University Research Colloquium 2020:371–8.

Kemenkes RI. Peraturan Menteri Kesehatan Nomor 6 Tahun 2016 tentang Formularium Obat Herbal Asli Indonesia. Database Peraturan | JDIH BPK 2016. http://peraturan.bpk.go.id/Details/112998/permenkes-no-6-tahun-2016 (accessed October 12, 2023).

Prabaningdyah NK, Riyanto S, Rohman A, Siregar C. Application of HPLC and response surface methodology for simultaneous determination of curcumin and desmethoxy curcumin in Curcuma syrup formulation. Journal of Applied Pharmaceutical Science 2017;7:058–64. https://doi.org/10.7324/JAPS.2017.71207. DOI: https://doi.org/10.7324/JAPS.2017.71207

Hanwar D, Anwar ’Aina Fahrina, Suhendi A. Pengawasan Mutu Produk Obat Herbal Berbasis Curcuma sp. Dengan Parameter Kadar Kurkumin Menggunakan Metode Kromatografi Cair Kinerja Tinggi. Prosiding University Research Colloquium 2021:973–80.

Watson DG. Pharmaceutical analysis: a textbook for pharmacy students and pharmaceutical chemists. Fourth edition. Edinburgh; New York: Elsevier; 2017.

Chrissanti RD, Darmawati A, Yuwono M. Optimasi Metode KCKT untuk Penetapan Kadar 4-Isobutilasetofenon dan 2-(4-Isobutirilfenil) Asam Propanoat dalam Tablet Ibuprofen. JFIKI 2020;7:26. https://doi.org/10.20473/jfiki.v7i12020.26-34. DOI: https://doi.org/10.20473/jfiki.v7i12020.26-34

Erpina E, Rafi M, Darusman L, Vitasari A, Putra B, Rohaeti E. Simultaneous Quantification of Curcuminoids and Xanthorrhizol in Curcuma Xanthorrhiza by High-Performance Liquid Chromatography. Journal of Liquid Chromatography & Related Technologies 2017;40. https://doi.org/10.1080/10826076.2017.1343729. DOI: https://doi.org/10.1080/10826076.2017.1343729

Kusumadewi AP, Mujahid R, Martien R, Purwanto P, Styawan AA, Irnawati I, et al. Analysis of Curcumin Contents in Curcuma xanthorrhiza using FTIR Spectroscopy and HPLC-UV in Combination with Multivariate Calibration. Traditional Medicine Journal 2023;28:171–7. https://doi.org/10.22146/mot.82220. DOI: https://doi.org/10.22146/mot.82220

Bose A. HPLC Calibration Process Parameters in Terms of System Suitability Test. Austin Chromatography 2014;1.

Riyanto. Validasi & Verifikasi Metode Uji: Sesuai Dengan Iso/Iec17025 Laboratorium Pengujian Dan Kalibrasi. 3rd ed. Yogyakarta: Deepublish; 2016.

USP Convention. The United States pharmacopeia 46. Rockville, MD: United States Pharmacopeial Convention; 2023.

Muryanto M. Validasi Metode Analisa Amonia pada Air Tanah Menggunakan Metode Spectrofotometri. IJL 2020;2:40. https://doi.org/10.22146/ijl.v2i1.54490. DOI: https://doi.org/10.22146/ijl.v2i1.54490

Moldoveanu Șerban, David V. Selection of the HPLC method in chemical analysis. 1st ed. Amsterdam, Netherlands; Cambridge, MA, United States: Elsevier; 2017.

Wahyuningtyas SEP. Pengaruh Jenis Pelarut Terhadap Kandungan Senyawa Kurkumin Dan Aktivitas Antioksidan Ekstrak Kunyit (Curcuma domestica Val.). Jurnal Ilmu dan Teknologi Pangan 2017;6:61–70.

Shary AK, Mahfur M. Skrining Fitokimia Dan Uji Kadar Kurkumin Pada Fraksi Etil Asetat Rimpang Kunyit (Curcuma Longa) Dengan Metode Klt Dan Spektrofotometri Uv-Vis. Pena: Jurnal Ilmu Pengetahuan dan Teknologi 2023;37:111–8. https://doi.org/10.31941/jurnalpena.v37i2.3080. DOI: https://doi.org/10.31941/jurnalpena.v37i2.3080

Kadam PV, Yadav KN, Bhingare CL, Patil MJ. Standardization and quantification of curcumin from Curcuma longa extract using UV visible spectroscopy and HPLC. J Pharmacogn Phytochem 2018;7:1913–8.

Anggraini YD, Anwar K, Budiarti A. Analisis Kadar Hidrokuinon dan Asam Kojic Dalam Tiga Merek Dagang Body Lotion Menggunakan Kromatografi Cair Kinerja Tinggi. Jurnal Ilmiah Sains 2023;23:20–30. https://doi.org/10.35799/jis.v23i1,45703.

Trisnawati NN, Suari PPV, Krismayanti NPA. Validasi Metode Uji Merkuri Menggunakan Inductively Coupled Plasma Emission Spectrometry (ICPE) 9000. Journal of Applied Chemistry 2021;9.

Korany MA, Haggag RS, Ragab MAA, Elmallah OA. A validated stability-indicating HPLC method for simultaneous determination of Silymarin and Curcumin in various dosage forms. Arabian Journal of Chemistry 2017;10:S1711–25. https://doi.org/10.1016/j.arabjc.2013.06.021. DOI: https://doi.org/10.1016/j.arabjc.2013.06.021

Eneş D, Çelebi̇Er M, Di̇Kmen D, Altinöz S. RP-HPLC Method Development for Determination of Curcumin in Commercial Turmeric Capsules. HUJPHARM 2023. https://doi.org/10.52794/hujpharm.1253858. DOI: https://doi.org/10.52794/hujpharm.1253858