Determination of Total Flavonoid Content from the Ethyl Acetate Fraction of Soursop Leaves (Annona muricata L.) Using the UV-Vis Spectrophotometry Method
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Nadia Ananda, Ainil Fithri Pulungan, Haris Munandar Nasution, Anny Sartika Daulay (2025). Determination of Total Flavonoid Content from the Ethyl Acetate Fraction of Soursop Leaves (Annona muricata L.) Using the UV-Vis Spectrophotometry Method. Journal of Pharmaceutical and Sciences, JPS Volume 8 Nomor 3 (2025). https://doi.org/10.36490/journal-jps.com.v8i3.1023
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Abstract
Background: The use of plants as traditional medicine is supported by their bioactive compounds, particularly secondary metabolites such as flavonoids. Soursop leaves (Annona muricata L.) are known to be rich in these compounds; however, quantitative data, especially in specific fractions, remain limited. Determining the total flavonoid content is essential for the standardization of herbal raw materials. Objective: This study aimed to identify the classes of chemical compounds present in the ethanol extract and ethyl acetate fraction of soursop leaves and to determine the total flavonoid content of both samples. Methods: Soursop leaf simplicia was extracted using 70% ethanol by the maceration method. The resulting ethanol extract was then fractionated with ethyl acetate. Phytochemical screening was conducted to identify compound classes, while the determination of total flavonoid content was quantitatively analyzed using the UV-Vis spectrophotometry method with quercetin as the standard. Results: Phytochemical screening revealed that both the ethanol extract and the ethyl acetate fraction tested positive for alkaloids, flavonoids, tannins, and steroids/triterpenoids. The total flavonoid content obtained in the ethanol extract was 31.524 ± 0.1524 mg QE/g, while the ethyl acetate fraction showed a significantly higher content of 41.425 ± 0.1363 mg QE/g. Conclusion: Fractionation with ethyl acetate effectively concentrated flavonoid compounds from the crude soursop leaf extract, as indicated by the higher total flavonoid content in the ethyl acetate fraction. These findings support the potential of the ethyl acetate fraction of soursop leaves as a source of flavonoids for the development of standardized herbal medicines.
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Harnita N, Putri AM, Fitria D, Fajriati M. Identifikasi Metabolit Sekunder Ekstrak N-Heksana dari Daun Sirsak (Annona muricata L). JSSIT J Sains Dan Sains Terap 2024;2:1–5.
Sahrianti N, Mastura AA. Skrining Fitokimia Senyawa Metabolit Sekunder Daun Sirsak (Annona muricata L.) di Kabupaten Majene, Mamuju dan Mamuju Tengah. J Ilm Farm Simplisia 2023;3:161–8.
Carole NC, Ekpe IP, Ifeanacho NW, Erhijakpor O, Damilola E, Juachi E. Evaluation of Phytochemical Profile and Comparative Free Radical Scavenging Activities of Ethanolic Extract of Annona Muricata Leaf and Fruit. Int Res J Mod Eng Technol Sci 2022. https://doi.org/10.56726/irjmets29737.
Faradiba F, Amin A, Sukmawati S, Achmad CA, Ananda R, Saputri D, et al. Total Flavonoid Content and Antioxidant Activity of Soursop Leaves From the Three Largest Producing Areas of South Sulawesi Province, Indonesia. Wind Heal J Kesehat 2024:400–12. https://doi.org/10.33096/woh.v7i4.1385.
Birendra S, Ramappa H, Kotha S, M RR, Siddamsetty RS. Pharmacognostic Evaluation of Fruits and Leaves of Annona Muricata L. Int J Pharm Investig 2020;10:86–92. https://doi.org/10.5530/ijpi.2020.1.16.
Kurang RY, Adang B. Skrining Fitokimia Dan Uji Aktivitas Antioksidan Daun Sirsak (Annona Muricata L) Dengan Metode 1, 1-Difenil-2-Pikrylhidrazyl (Dpph). Partner 2018;23:567–74.
Ginting B, Yahya M, Saidi N, Maulana I, Murniana M, Safitri E, et al. Antioxidant and Cytotoxicity Screenings of Ethyl Acetate Extract From Annona Muricata Leaves and Its Fractions. J Adv Pharm Technol Amp Res 2024;15:70–4. https://doi.org/10.4103/japtr.japtr_470_23.
Suhaenah A, Pratama M, Amir AHW. Penetapan Kadar Flavonoid Fraksi Etil Asetat Daun Karet Kebo (Ficus elastica) dengan metode spektrofotometri UV-VIS. As-Syifaa J Farm 2021;13:48–54.
Bangun PPA, Rahman AP. Analisis kadar total flavonoid pada daun dan biji pepaya (Carica papaya L.) menggunakan metode spektrofotometer UV-vis. J Ilm Farm Attamru 2021;2:1–5.
Yani NKLP, Nastiti K, Noval N. Pengaruh Perbedaan Jenis Pelarut Terhadap Kadar Flavonoid Total Ekstrak Daun Sirsak (Annona muricata L.): The Effect of Different Types of Solvents on Total Levels of Flavonoid Extract (Annona muricata L.). J Surya Med 2023;9:34–44.
Qorina F, Arsianti A, Fithrotunnisa Q, Tejaputri NA. Phytochemistry and Antioxidant Activity of Soursop (Annona Muricata) Leaves. Int J Appl Pharm 2019:1–6. https://doi.org/10.22159/ijap.2019.v11s6.33524.
Rahmawati R, Muflihunna A, Kusuma AT. Analisis Kadar Flavonoid dan Fenolik Total Fraksi Etil Asetat Daun Ketepeng Cina (Senna Alata (L.) Roxb) DENGAN Metode Spektrofotometri Uv-Visible. As-Syifaa J Farm 2015;7:10–8. https://doi.org/10.33096/ja.v7i1.16.
Anggraeni R. Uji Karakteristik Simplisia Buah Andaliman (Zanthoxylum acanthopodium DC.). JIFI (Jurnal Ilm Farm Imelda) 2020;3:32–8. https://doi.org/10.52943/jifarmasi.v3i2.210.
Sari RP, Laoli MT. Karakterisasi Simplisia Dan Skrining Fitokimia Serta Analisis Secara Klt (Kromatografi Lapis Tipis) Daun Dan Kulit Buah Jeruk Lemon (Citrus Limon (L.) Burm. F.). JIFI (Jurnal Ilm Farm Imelda) 2019;2:59–68.
DepKes R. Farmakope Indonesia Edisi Ketiga 1979:33.
Sarker SD, Latif Z, Gray AI. Methods in Biotechnology, Natural products isolation. Hum Press Nothern Ireland-United Kingdom 529pp Spampinato, C Leonardi, D(2013) Candida Infect Causes, Targets, Resist Mech Tradit Altern Antifung Agents J Biomed Res Int 2006;2013:1–13.
Mayasari U, Laoli MT. Karakterisasi simplisia dan skrining fitokimia daun jeruk lemon (citrus limon (l.) burm. f.). KLOROFIL J Ilmu Biol Dan Terap 2018;2:7–13.
Anggraeni R. Uji karakteristik simplisia buah andaliman (Zanthoxylum acanthopodium DC.). J Ilm Farm Imelda 2019;3:34–40.
Aminah A, Tomayahu N, Abidin Z. Penetapan Kadar Flavonoid Total Ekstrak Etanol Kulit Buah Alpukat (Persea americana Mill.) Dengan Metode Spektrofotometri UV-Vis. J Fitofarmaka Indones 2017;4:226–30. https://doi.org/10.33096/jffi.v4i2.265.
Salim M, Sulistyaningrum N, Isnawati A, Sitorus H, Yahya, Ni’mah T. Karakterisasi Simplisia dan Ekstrak Kulit Buah Duku ( Lansium domesticum Corr ) dari Provinsi Sumatera Selatan dan Jambi. J Kefarmasian Indones 2016;6:117–28.
Mukhriani M. Farmakognosi Analisis 2014.
Setyowati E, Sujono TA. Aktivitas antidiabetes melitus ekstrak kulit buah jeruk manis (Citrus sinensis) dan kulit buah kelengkeng (Euphoria longan (Lour.) Steud) terhadap tikus putih jantan galur wistar yang diinduksi aloksan 2014.
Sangi M, Runtuwene MRJ, Simbala HEI, Makang VMA. Analisis fitokimia tumbuhan obat di Kabupaten Minahasa Utara. Chem Prog 2019;1:47–53.
Wahid AR, Safwan S. Efek Antioksidan Ekstrak Etanol Daun Gaharu (Aquilaria Malaccensis L.) Pada Tikus Jantan Galur Sprague Dawley Yang Diinduksi Paracetamol (Kajian Aktivitas Enzim Katalase, SGOT Dan SGPT). Pharmauho J Farm Sains, Dan Kesehat 2019;4.
Suharyanto S, Prima DAN. Penetapan kadar flavonoid total pada juice daun ubi jalar ungu (Ipomoea batatas L.) yang berpotensi sebagai hepatoprotektor dengan metode spektrofotometri uv-vis. Cendekia J Pharm 2020;4:110–9.
Chang C-C, Yang M-H, Wen H-M, Chern J-C. Estimation of total flavonoid content in propolis by two complementary colorimetric methods. J Food Drug Anal 2002;10.
S.Salim N. Effect of Ethanolic Leaves Extracts of Annona Muricata. Linn on Hyperlipidemic Rats. Ann Agric Sci Moshtohor 2021;59:339–48. https://doi.org/10.21608/assjm.2021.194820.
Weliyanto Y, Hakim AR, Hidayah N. Penetapan Kadar Flavonoid Total Berdasarkan Tingkat Fraksinasi Ekstrak Daun Sirsak (Annona muricata L.): Determination Of Total Flavonoid Content Based On Soursop Leaf Extract Fractionation Level (Annona muricata L.). J Surya Med 2025;11:114–9. https://doi.org/10.33084/jsm.v11i2.10537.