Comparison of Antioxidant Activity of Flavonoid Compounds From (Moringa Oleifera L.) Leaf Extract Using Uv-Vis Spectrophotometry Method
Article Sidebar
Main Article Content
Page: 1177-1183
Abstract
The bioactive compounds found in moringa leaves (Moringa oleifera L.) can function as natural antioxidants to protect cells from damaging free radicals. The purpose of this study is to ascertain the antioxidant activity of moringa leaves according to the type of solvent, analyze the relationship between antioxidant capacity and concentration of active compounds, and determine the best solvent. Extraction was carried out using ethanol, methanol, and aquades solvents, followed by testing antioxidant activity using UV-Vis spectrophotometry method on DPPH radicals. The content of phenols, flavonoids, and tannins in the extract was measured to understand their contribution to antioxidant activity Based on the results of the study, the extract containing ethanol solvent showed the most powerful antioxidant activity, with an IC50 value of 103.98 ppm. In conclusion, the type of solvent has a significant effect on the antioxidant activity of moringa leaves, with ethanol solvents being the most effective option. This research strengthens the potential of moringa leaves as a natural source of antioxidants that can be utilized for applications in the pharmaceutical and health fields, although further research is needed to optimize extraction techniques and the development of moringa oleifera leaf-based product
Downloads
Article Details
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
References
Alfaridz, F., & Amalia, R. (2019). Review jurnal: Klasifikasi dan aktivitas farmakologi dari senyawa aktif flavonoid. Farmaka, 3, 1–9.
Al Kausar, R., Putra, A. S., & Tutik. (2023). The Relationship Between Flavonoid Content And Antioxidant Activity In Guava Leaves (Syzygium aqueum) And Moringa Leaves (Moringa oleifera) Using Uv-Vis Spectrophotometry. Jurnal Analis Farmasi, Vol.8(2), 170-187.
Bhutto AA, Kalay S, Sherazi STH, Culha M. (2018). Quantitative structure–activity relationship between antioxidant capacity of phenolic compounds and the plasmonic properties of silver Nanoparticles. 189:174–81.
Bratovcic, A. (2020). Antioxidant enzymes and their role in preventing cell damage. Acta Sci Nutr Heal. 4(3):1–7.
Charoensin, S., (2014). Antioxidant and anticancer activities of Moringa oleifera leaves. Journal of Medicinal Plant Research, Vol. 8(7), 318-325.
Halifa, D. Z., Ahmad, A. R., & Hasnaeni. (2024). Uji Aktivitas Antioksidan Ekstrak Deklorofilasi Daun Kelor (Moringa oleifera Lam.) Dengan Metode DPPH. Makassar Natural Product Journal, 33-42.
Hasanah, N., Susilo, J., & Oktianti, D. (2017). Uji Aktivitas Antioksidan Ekstrak Etanol Daun Kelor (Moringa oleifera lam) Dengan Metode DPPH. Jurnal Gizi dan Kesehatan.
International Council for Harmonisation of Technical Requirements for Pharmaceuticals for Human Use (ICH). ICH Q2(R1): Validation of Analytical Procedures: Text and Methodology. Geneva: ICH; 2005.
Kumar V, Abbas AK, & Aster JC. (2020). Robbins & cotran pathologic basis of disease. 10th ed. Philadelphia: Elsevier.
Kurang, R. Y., Koly, F. V., & Kafolapada, D.I,. (2020). Aktivitas Antioksidan Ekstrak Etil Asetat Daun Kelor (Moringa Oleifera L) . Journal of Pharmaceutical Care Anwar Medika.
Leone A, Spada A, Battezzati A, Schiraldi A, Aristil J, Bertoli S. Moringa oleifera seeds and oil: Characteristics and uses for human health. Plos one. 2016;10(6)
Muna, L. N. (2022). Aktivitas antioksidan ekstrak air daun kelor (Moringa oleifera) dengan metode DPPH serta analisis kualitatif kandungan metabolit sekunder. Sasambo Journal of Pharmacy.
Musdja MY, Rahman HA, Hasan. Antioxidant activity of catechins isolate of uncaria gambier roxb in male rats. Int J Heal Life-Sciences. 2018; 4(2):34–43.
Noer, S., Pratiwi, R. D., & Gresinta, E. (2018). Penetapan kadar senyawa fitokimia (tanin, saponin dan flavonoid) sebagai kuersetin pada ekstrak daun inggu (Ruta angustifolia L.). Jurnal Eksakta, 18(1), 19–29.
Pandey, K. B, & Rizvi S. I. Plant polyphenols as dietary antioxidants in human health and disease. Oxid Med Cell Longev. 2009;2(5):270–278.
Satriyani, D. P. (2021). Review Artikel: Aktivitas Antioksidan Ekstrak Daun Kelor (Moringa oleifera Lam). Jurnal Farmasi Malahayati, 4(1).
Segwatibe, M. K., Cosa, S., & Bassey, K. (2023). Antioxidant and antimicrobial evaluations of Moringa oleifera Lam leaves extract and isolated compounds. Molecules, 28(2), 899.
Susanty, Ridnugrah, N. A., Chaerrudin, A., & Yudistirani, S. A. (2019). Aktivitas Antioksidan Ekstrak Daun Kelor (Moringa oleifera) Sebagai Zat Tambahan Pembuatan Moisturizer. Jurnal Universitas Muhammadiyah Jakarta.
Syafriah, W.O., & Teheni, M.T. (2023). Uji Aktivitas Antioksidan Ekstrak Metanol Tangkai Daun Kelor (Moringa oleifera Lam) Menggunakan Metode DPPH (1,1-difenil-2-pikrilhidrazil). Jurnal Promotif Preventif, Vol.6(6), 953-960
Trugo LC, von Baer E, von Baer D. (2016). Lupin breeding. Reference Module in Food Science. 2nd ed. Elsevier Ltd. (2):7.
Tutik, Dwipayana, I. A., & Elsyana, V. (2018). Identifikasi Dan Perbandingan Aktivitas Antioksidan Ekstrak Daun Kelor Pada Variasi Pelarut Dengan Metode DPPH. Jurnal Farmasi Malahayati, Vol.1(2)
Wang, T., Li, Q., & Bi, K. S. (2018). Bioactive flavonoids in medicinal plants: Structure, activity and biological fate. Asian Journal of Pharmaceutical Sciences, 13(1), 12–23.
Wijana, S., Sucipto, dan L. M. Sari. (2014). Pengaruh Suhu dan Waktu Pengeringan Terhadap Aktivitas Antioksidan pada Bubuk Kulit Manggis (Garcinia mangostana L.). Tesis. Fakultas Teknologi Pertanian Universitas Brawaijaya.
Zulkifli, S. A., Gani, S. S. A., Zaidan, U. H., & Halmi, M. I. E. endi. (2020). Optimization of Total Phenolic and Flavonoid Contents of Defatted Pitaya (Hylocereus polyrhizus) Seed Extract and Its Antioxidant Properties. Molecules, 25, 1–17.