Analysis of Chlorine Levels in Rice (Oryza sativa L.) Circulating in Several Markets in Medan City Using the UV-Vis Spectrophotometry Method
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Atilla Eliza Putri Suhartono, Ainil Fithri Pulungana, Ridwanto, Anny Sartika Daulay (2026). Analysis of Chlorine Levels in Rice (Oryza sativa L.) Circulating in Several Markets in Medan City Using the UV-Vis Spectrophotometry Method. Journal of Pharmaceutical and Sciences, JPS Volume 9 Nomor 4 (2026). https://doi.org/10.36490/journal-jps.com.v9i4.1872
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Background: Rice quality assessment requires analytical evidence to distinguish potentially chlorine-reactive residues from ordinary physical characteristics. Objective: To examine colorimetric responses and estimate chlorine-equivalent levels in rice sold at three markets in Medan. Methods: Ten white-rice samples were selected purposively from Simpang Limun, Sukaramai, and Petisah markets. Aqueous extracts were examined using N,N-diethyl-p-phenylenediamine (DPD), potassium iodide–starch, silver nitrate, and povidone-iodine reagents. Five DPD-reactive samples were analysed by visible-range spectrophotometry at 510 nm, with six instrumental readings per sample. Results: Samples A–E (50%) produced pink and blue responses in the DPD and iodide–starch tests, respectively; samples F–J remained colourless in both tests. Calibration at nominal concentrations of 0–60 µg/mL yielded R² = 0.9972. Conditional semiquantitative estimates for A–E were 182.99, 220.57, 69.72, 75.12, and 84.00 µg/g, espectively. All samples were white and had a characteristic rice odour. Conclusion: Five samples exhibited oxidant responses consistent with active chlorine. Incomplete matrix validation and an unverified mass-conversion factor limit quantitative interpretation and preclude definitive conclusions about chlorine content or food-safety compliance.
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References
Kementerian Kesehatan Republik Indonesia. Peraturan Menteri Kesehatan Nomor 33 Tahun 2012 tentang Bahan Tambahan Pangan. Jakarta: Kementerian Kesehatan Republik Indonesia; 2012.
Badan Pengawas Obat dan Makanan Republik Indonesia. Peraturan Badan Pengawas Obat dan Makanan Nomor 11 Tahun 2019 tentang Bahan Tambahan Pangan. Jakarta: BPOM; 2019.
Widada A, Mualim M, Gazali M. Analisis kandungan klorin (Cl2) pada beras yang beredar di Pasar Kota Bengkulu. Mitra Raflesia 2023;15(1). https://doi.org/10.51712/mitraraflesia.v15i1.159.
Warsani Z, Sholihah K. Identifikasi kandungan klorin pada beras putih (Oryza sativa L.) yang beredar di Pasar Tradisional Masbagik Kabupaten Lombok Timur. J Sains Teknol 2023;6(3):356–64.
Fitriani F, Raharjo P, Harnani Y, Kamalizaman MK, Wahyuni D. Analisa klorin pada beras yang beredar di Pasar Tradisional Cik Puan Kota Pekanbaru tahun 2020. Media Kesmas (Public Health Media) 2022;2(1):94–101. https://doi.org/10.25311/kesmas.vol2.iss1.11.
Aminah S, Marzuki I, Rasyid A. Analisis kandungan klorin pada beras yang beredar di Pasar Tradisional Makassar dengan metode argentometri Volhard. Seminar Nasional Pangan, Teknologi, dan Entrepreneurship; 2019.
Alfitri O, Dhanti KR, Wardani DPK. Analisis senyawa klorin (Cl2) pada beras yang dijual di Pasar Sokaraja Kabupaten Banyumas. J Kesehatan 2021;14(1). https://doi.org/10.23917/jk.v14i1.12418.
Asrina R, Anganria J. Analisis kualitatif klorin (Cl2) pada beras putih yang beredar di Pasar Tradisional Daya Kota Makassar. J Farmasi Sandi Karsa 2019;5(1). https://doi.org/10.36060/jfs.v5i1.32.
American Public Health Association, American Water Works Association, Water Environment Federation. Standard Methods for the Examination of Water and Wastewater: 4500-Cl G, DPD colorimetric method [Internet]. Washington (DC): APHA; [diakses 19 Oktober 2026]. https://www.nemi.gov/methods/method_summary/7431/.
Zhou M, Li T, Zu M, Zhang S, Liu Y, Zhao H. Membrane-based colorimetric flow-injection system for online free chlorine monitoring in drinking water. Sens Actuators B Chem 2021;327:128905. https://doi.org/10.1016/j.snb.2020.128905.
Jain A, Verma KK. Extractive reactions in embedded drops for enhanced selectivity in the determination of chloride and free chlorine by micro-spectrophotometry using N,N-diethyl-p-phenylenediamine. Microchem J 2025;208:112373. https://doi.org/10.1016/j.microc.2024.112373.
International Council for Harmonisation. ICH Q2(R2): Validation of Analytical Procedures. Final version adopted 1 November 2023. Geneva: ICH; 2023.
Cantwell H, editor. The Fitness for Purpose of Analytical Methods: A Laboratory Guide to Method Validation and Related Topics. 3rd ed. Eurachem; 2025.
Walls RM, Hockberger RS, Gausche-Hill M, Erickson TB, Wilcox SR, editors. Rosen’s Emergency Medicine: Concepts and Clinical Practice. 10th ed. Philadelphia: Elsevier; 2023.
Agency for Toxic Substances and Disease Registry. Toxicological Profile for Chlorine. Atlanta (GA): US Department of Health and Human Services; 2010.
World Health Organization. Chemical fact sheets: chlorine. In: Guidelines for drinking-water quality, fourth edition incorporating the first and second addenda. Geneva: World Health Organization; 2022.
Apriani, Latifani R. Identifikasi klorin pada tepung terigu (bermerek dan tidak bermerek) dan tepung beras (bermerek). J Health Sains 2020;1(6). https://doi.org/10.46799/jhs.v1i6.64.
Elfia M. Analisis klorin pada bihun yang dijual di Pasar Kodim Pekanbaru. Ensiklopedia of Journal 2020;2(4):40–5.
Harris DC, Lucy CA. Quantitative Chemical Analysis. 11th ed. Macmillan Learning; 2026.
Harvey D. Analytical Chemistry 2.1 [Internet]. Chemistry LibreTexts; [diakses 19 Oktober 2026]. https://chem.libretexts.org/Bookshelves/Analytical_Chemistry/Analytical_Chemistry_2.1_by_David_Harvey/Analytical_Chemistry_2.1_(Harvey).
Skoog DA, Holler FJ, Crouch SR. Principles of Instrumental Analysis. 7th ed. Boston: Cengage Learning; 2018.
Sudarma N, Idayani S, Setiawan D, Dharmawan PO. Pemanfaatan Betadine sebagai indikator uji klorin pada beras berpemutih. Bali Medika J 2018;5(2):157–64. https://doi.org/10.36376/bmj.v5i2.32.