PHYTOCHEMICAL SCREENING AND ANTIOXIDANT ACTIVITIES OF KEMANGI LEAF (Ocimum tenuiflorum L.) METHANOL EXTRACT USING DPPH (1,1-diphenyl-2-pikrilhidrazil) METHOD
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Boy Chandra, Rezza Puspita Sari, Sestry Misfadhila, Zikra Azizah, Ridho Asra (2019). PHYTOCHEMICAL SCREENING AND ANTIOXIDANT ACTIVITIES OF KEMANGI LEAF (Ocimum tenuiflorum L.) METHANOL EXTRACT USING DPPH (1,1-diphenyl-2-pikrilhidrazil) METHOD. Journal of Pharmaceutical and Sciences, Vol. 2 No. 2 (2019): JPS Volume 2 Nomor 2 (2019). https://doi.org/10.36490/journal-jps.com.v2i2.20
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Abstract
Research on phytochemical tests and antioxidant activity of methanol extract of basil leaves (Ocimum tenuiflorum L.) was carried out. Simplicia of basil leaves macerated with 98 % methanol obtained an extract of 45.5479 g with a yield of 22.77 %. Methanol extract of basil leaves contains alkaloid, flavonoid, phenol, tannin, saponin, and steroid. The extract is known to have antioxidant activity by decreasing DPPH absorptions. The antioxidant activity of methanol extract of basil leaves was carried out using the DPPH method for determination of IC50.Values from the test it can be seen that basil leaf extract has antioxidant activity with an IC50 value of 13.7092 ppm. Based on IC50 values showed that the methanol extract of basil leaves has very weak antioxidant activity.
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Banu, K. S., & Cathrine, L. (2015). General techniques involved in phytochemical analysis. International Journal of Advanced Research in Chemical Sciene, 2(4), 25-32.
Departemen Kesehatan Republik Indonesia. (1995). Materia medika Indonesia. (Edisi VI). Jakarta: Departemen Kesehatan Republik Indonesia.
Departemen Kesehatan Republik Indonesia. (2008). Farmakope Herbal Indonesia. (Edisi 1). Jakarta: Departemen Kesehatan Republik Indonesia.
Dinata, D. I., Supriadi, D., Djafar. G., Syerliana., Wijayanti. W., & Suherman, S. E. (2015). Effect of adding granul basil (Ocimum americanum) as antioxidant in fried foods. IJPST, 2(1), 22-32.
Hanani, E., Mun’im, A., & Sekarini, R. (2005). Identifikasi senyawa antioksidan dalam spons Callyspongia sp, dari Kepulauan Seribu. Majalah Ilmu Kefarmasian. 2(3), 127-133.
Hanani, E. (2017). Analisis fitokimia. Jakarta: EGC.
Harborne, J. B. (1987). Metode fitokimia, penuntun cara modern menganalisis tumbuhan. (Edisi ke-2). Penerjemah: K. Padmawinata dan I. Soediro. Bandung: ITB.
Kementerian Kesehatan Republik Indonesia. (2014). Farmakope Indonesia (Edisi V). Jakarta: Kementerian Kesehatan Republik Indonesia.
Molyneux, P. (2004), The use of stable free diphenylpicryllhydrazyl (DPPH) For estimating antioxsidant activity. Songklanakarin J. Scitechnol. 26(2): 50-55.
Mosquera, O. M. Correa, Y. M. Buitrago, D. C. & Nino, J. (2007). Antioxsidant Activity Of Twenty Five Plant from Colombian Biodeivesity. Mem Inst Oswaldo Cruz, Rio de janeiro. 102 (5): 631-634.
Sangi, M. S., Momuat, L. I., & Kumaunang, M., (2013). Uji toksisitas dan skrining fitokimia tepung gabah pelepah aren (Arange pinnata). Manado: Universitas Sam Ratulangi.
Sarma, D. S. K & Babu, A. V. S. (2011). Pharmacognostic and phytochemical studies of Ocimum americanum. Journal of Chemical and Pharmaceutical Research. 3(3): 337-347.
Sayuti, K., & Yenrina, R. (2015). Antioksidan alami dan antioksidan sintetik. Padang: Universitas Andalas.
Sharma, R & Kumar B.S. (2013). Isolation characterization and antioxidant potential of endophytic fungi of Ocimum sanctum Linn. (Lamiaceae). Indian Journal of applied research, 3(7), 5-10.
Sunarni, T., Pramono, S. & Asmah, R. (2007). Flavonoid antioksidan penangkap radikal dari daun kepel (Stelechocarpus burahol (BI.) Hook f. & Th.). Majalah Farmasi Indonesia, 18(3), 111-116.
Winarsih, H. (2007). Antioksidan alami dan radikal bebas. Jogjakarta: Kanisius.