Uji Permeabilitas Formulasi Self-Nanoemulsifying Drug Delivery Systems (SNEDDS) Asiaticoside Ekstrak Etanol Daun Pegagan (Centella asiatica L.) dengan Metode Usus Terbalik
Statistik artikel
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Tri Buana Tungga Dewi, Gunawan Setiyadi (2026). Uji Permeabilitas Formulasi Self-Nanoemulsifying Drug Delivery Systems (SNEDDS) Asiaticoside Ekstrak Etanol Daun Pegagan (Centella asiatica L.) dengan Metode Usus Terbalik. Journal of Pharmaceutical and Sciences, JPS Volume 9 Nomor 4 (2026). https://doi.org/10.36490/journal-jps.com.v9i4.1854
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Abstrak
Asiaticoside merupakan senyawa aktif daun pegagan (Centella asiatica L.) yang memiliki kelarutan rendah dan bobot molekul relatif tinggi sehingga dapat membatasi absorpsinya. Penelitian ini bertujuan mengetahui pengaruh Self-Nanoemulsifying Drug Delivery System (SNEDDS) terhadap permeabilitas asiaticoside ekstrak etanol pegagan menggunakan metode usus terbalik. Penelitian dilakukan pada 10 ekor tikus putih jantan yang dibagi menjadi kelompok ekstrak dengan SNEDDS dan ekstrak tanpa SNEDDS. Kadar asiaticoside pada cairan serosal ditentukan menggunakan kromatografi cair kinerja tinggi. Hasil penelitian menunjukkan nilai permeabilitas kelompok SNEDDS sebesar 3,13 × 10⁻³ cm/menit, sedangkan kelompok tanpa SNEDDS sebesar 0,74 × 10⁻³ cm/menit, dengan enhancement ratio sebesar 3,97. Nilai lag time kelompok SNEDDS sebesar 18,17 menit dan kelompok tanpa SNEDDS sebesar 22,42 menit. Data dianalisis menggunakan uji normalitas Shapiro–Wilk, kemudian dilanjutkan dengan uji nonparametrik. Namun, hasil uji Mann–Whitney menunjukkan bahwa tidak terdapat perbedaan yang signifikan antara kelompok SNEDDS dan ekstrak tanpa SNEDDS pada parameter permeabilitas maupun lag time (p > 0,05). Hasil yang tidak signifikan tersebut diduga disebabkan oleh tingginya variabilitas antarindividu pada hewan uji, serta jumlah sampel yang relatif kecil. Dengan demikian, sistem SNEDDS menunjukkan kecenderungan meningkatkan permeabilitas dan penurunan lag time asiaticoside secara deskriptif, tetapi efek tersebut belum didukung oleh bukti statistik yang signifikan pada kondisi penelitian ini
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Referensi
Wong JH, Barron AM, Abdullah JM. Mitoprotective Effects of Centella asiatica (L.) Urb.: Anti-Inflammatory and Neuroprotective Opportunities in Neurodegenerative Disease. Front Pharmacol 2021;12:1–9. https://doi.org/10.3389/fphar.2021.687935.
Chairunisa U, Rosaini H, Musa ZFA, Makmur I, Eriadi A, Yetti RD. Optimization of Nanoemulsion Formula from Asiaticoside Active Compounds Using Design Expert ®13. Int J Res Publ Rev 2022;3:1046–51. https://doi.org/10.55248/gengpi.2022.3.10.44.
Sabila CF, Muhartono. Efektivitas Pemberian Ekstrak Daun Pegagan (Centella asiatica) terhadap Penyembuhan Luka The Effectivity of Giving Gotu Kola Leaf Extract (Centella asiatica) to Wound Healing. J Agromedicine Unila 2020;7:23–8. https://juke.kedokteran.unila.ac.id/index.php/agro/article/view/2776.
Mardiyah S. Anti-Inflammatory Activity Of Pegagan Leaves (Centella asiatica (L.) Urban) Extract Cream. J Pharm Sci Appl 2022;4:12–8. https://doi.org/10.24843/JPSA.2022.v04.i01.p02 herbs.
Fitrianda E, Sukandar EY, Elfahmi, Adnyana K. Antidiabetic Activity of Extract, Fractions, and Asiaticoside Compound Isolated From Centella Asiatica Linn. Leaves in Alloxan-Induced Diabetic Mice. Asian J Pharm Clin Res 2017;10:268–72. http://dx.doi.org/10.22159/ajpcr.2017.v10i10.20419.
Wardiyah, Jufri M, Sutriyo, Mun’im A, Zulfakar MH. Developement and Optimization of Asiaticoside Nanoemulsion For by Box-Behnken Design. Farmacia 2024;72:1397–407. https://doi.org/10.31925/farmacia.2024.6.19.
Chrobak E, Bober-Majnusz K, Wyszomirski M, Zięba A. Aniline Derivatives Containing 1-Substituted 1,2,3-Triazole System as Potential Drug Candidates: Pharmacokinetic Profile Prediction, Lipophilicity Analysis Using Experimental and In Silico Studies. MDPI Pharm 2024;17. https://doi.org/10.3390/ph17111476.
Ashfaq M, Shah S, Rasul A, Hanif M, Khan HU, Khames A, et al. Enhancement of the Solubility and Bioavailability of Pitavastatin through a Self-Nanoemulsifying Drug Delivery System (SNEDDS). Pharmaceutics 2022;14. https://doi.org/10.3390/pharmaceutics14030482.
Suyal J, Kumar B, Jakhmola V. Novel Approach Self-nanoemulsifying Drug Delivery System: A Review. Adv Pharmacol Pharm 2023;11:131–9. https://doi.org/10.13189/app.2023.110205.
Wikantyasning ER, Setiyadi G, Pramuningtyas R, Kurniawati MD, Ho CY. Formulation of Nanoemulgel Containing Extract of Impatients Balsamina L. and Its Antibacterial Activity. Int J Appl Pharm 2023;15:67–70. https://doi.org/10.22159/ijap.2023v15i3.46670.
Winarti L, Suwaldi, Martien R, Hakim L. Formulation of Self-Nanoemulsifying Drug Delivery System of Bovine Serum Albumin Using HLB (Hydrophilic-Lypophilic Balance) Approach. Indones J Pharm 2016;27:117–27. https://doi.org/10.14499/indonesianjpharm27iss3pp117.
Kus M, Ibragimow I, Piotrowska-Kempisty H. Caco-2 Cell Line Standardization with Pharmaceutical Requirements and In Vitro Model Suitability for Permeability Assays. Pharmaceutics 2023;15. https://doi.org/10.3390/pharmaceutics15112523.
Masitoh A, Sopyan I. Formulasi Nanopartikel Tanaman Herbal untuk Terapi Kanker. Maj Farmasetika 2019;4:165–72. https://doi.org/10.24198/farmasetika.v4i5.23111.
Setiyadi G. Pengembangan Nano Gamavution-0 Dengan Formulasi Self-Nanoemulifying Drug Delivery System (SNEDDS) Sebagai Kandidat Obat Antiinflamasi: Kajian In Vitro dan In Vivo. Universitas Gajah Mada, 2015.
Melati R, Setiyadi G. Formulation of Self-Nanoemulsifying Drug Delivery System (SNEDDS) from Ethanolic Extract of Pegagan Leaves (Centella asiatica) As An Oral Antidiabetic Drug. J Pharm Sci 2025;8:2362–71. https://doi.org/10.36490/journal-jps.com.
Anukunwithaya T, Tantisira M, Tantisira B, Khemawoot P. Pharmacokinetics of a Standardized Extract of Centella Asiatica ECa 233 in Rats. J Med Plant Nat Prod Res 2017;83:710–7. https://doi.org/10.1055/s-0042-122344.
Leary S, Pharmaceuticals F, Ridge H, Underwood W, Anthony R, Cartner S, et al. AVMA Guidelines for the Euthanasia of Animals : 2020 Edition *. American Veterinary Medical Association; 2020.
Buya AB, Beloqui A, Memvanga PB, Préat V. Self Nano Emulsifying Drug-Delivery Systems: From The Development To The Current Applications and Challenges in Oral Drug Delivery. Pharmaceutics 2020;12:1–52. https://doi.org/10.3390/pharmaceutics12121194.
Wang CY, Yen CC, Hsu MC, Wu YT. Self-nanoemulsifying Drug Delivery Systems for Enhancing Solubility, Permeability, and Bioavailability of Sesamin. MDPI Mol 2020;25. https://doi.org/10.3390/molecules25143119.
Costa CM, de Carvalho NM, de Oliveira DL, Madureira AR. A Critical Review on In Vitro and Ex Vivo Models of the Intestinal Epithelium of Humans and Monogastric Animals. Gastrointest Disord 2024;6:337–58. https://doi.org/10.3390/gidisord6010023.
Noorman L, Van Der Hee B, Gilbert MS, De Vries S, Van Der Hoek S, Gerrits WJJ. Assessing Seromuscular Layer and Serosa Removal on Intestinal Permeability Measurements in Weaned Piglet Everted Sac Segments. J Anim Sci 2024;102. https://doi.org/10.1093/jas/skae148.