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Elpa Giovana Zola
Barmi Hartesi
Lia Anggresani

Page: 1524-1532

Abstract

Background: Burns represent a condition of damaged or lost skin tissue due to high-temperature exposure. Oral therapy has limitations in penetrating hardened skin tissue, necessitating topical preparations such as gels that offer advantages in comfort, absorption capability, and ease of application. The stem of physic nut (Jatropha multifida L.) contains bioactive compounds including alkaloids, flavonoids, saponins, and tannins that may accelerate burn wound healing, particularly in its ethyl acetate fraction. Objective: This study aimed to formulate the ethyl acetate fraction of physic nut stem into a gel preparation and evaluate the effect of concentration variations on burn wound healing efficacy. Methods: Physic nut stems were extracted with 96% ethanol, then fractionated using n-hexane, ethyl acetate, and ethanol solvents. The ethyl acetate fraction was formulated into a hydrophilic gel at concentrations of 0.140%, 0.281%, and 0.421%. The gel preparations were evaluated through organoleptic tests, homogeneity, pH, spreadability, stability, irritation, and burn healing efficacy tests in rabbits. Results: Phytochemical screening revealed that the ethyl acetate fraction contained alkaloids, flavonoids, saponins, and tannins. All gel formulations met physical preparation requirements with skin-compatible pH (5.95-6.37). Formula F1 (0.140%) demonstrated optimal burn healing efficacy, equivalent to positive control (p > 0.05), and was topically safe (non-irritating). Conclusion: The ethyl acetate fraction of physic nut stem was successfully formulated into a stable and effective gel preparation for burn wound healing, with 0.140% as the optimal concentration. Further studies are required to examine toxicity and the mechanism of action of active compounds.

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How to Cite
Giovana Zola, E., Hartesi, B., & Anggresani, L. (2025). Formulation Gel Ethyl Acetate Fraction Stem Jarak Cina (Jattropha multifida L.) Effectiveness in Burn Healing. Journal of Pharmaceutical and Sciences, 8(3), 1524–1532. https://doi.org/10.36490/journal-jps.com.v8i3.966
Section
Original Articles

References

Rismana E, Rosidah I, Prasetyawan Y, Bunga O, Erna D, Pusat Y, et al. Efektivitas Khasiat Pengobatan Luka Bakar Sediaan Gel Mengandung Fraksi Ekstrak Pegagan Berdasarkan Analisis Hidroksiprolin Dan Histopatologi Pada Kulit Kelinci. Bul Penelit Kesehat 2013;41:45–60.

Departemen Kesehatan RI. Farmakope Indonesia. Jakarta: 1995.

Kuncari ES. Evaluasi, Uji Stabilitas Fisik Dan Sineresis Sediaan Gel Yang Mengandung Minoksidil, Apigenin Dan Perasan Herba Seledri (Apium graveolens L.). Bul Penelit Kesehat 2014;42:213–22.

Hariana A. Tumbuhan Obat dan Khasiatnya. (Seri 1). Jakarta :Swadaya 2006.

Sundaryono. Penggunaan Batang Tanaman Betadin (Jatropha mulitifida Linn) untuk Meningkatkan Jumlah Trombosit pada Mus musculus. Med Med Indones 2012;46:6–11.

Dewantari DR, Sugihartini N. Formulation and activity testing of Chinese petai leaf extract gel (Leucaena glauca, Benth) as a drug for burn. Farmasains 2015;2:217–22.

Aryunda D, Nasution MA, Nasution HM, Pulungan AF. Pengaruh Metode Ekstraksi Daun Jarak Cina (Jatropha multifida L.) Secara Maserasi dan Microwave-Assisted Extraction (MAE) Terhadap Aktivitas Antijamur Pada Candida albicans. J Pharm Sci 2025:868–81. DOI: https://doi.org/10.36490/journal-jps.com.v8i2.868

DIADARSONO A. Penggunaan Ekstrak Daun Jarak Cina (Jatropha multifida L), Ekstrak Lidah Buaya (Aloe vera), Dan Kombinasinya Terhadap Luka Sayat Tikus Putih (Rattus norvegicus) 2023.

Ulandari S. Uji Aktivitas Antibakteri Patch Ekstrak Batang Jatropha multifidaL. Terhadap Bakteri Staphylococcus aureus 2016.

Wiyono AE, Safitri IA, Rusdianto AS, Choiron M, Masahid AD. Optimization of the Combination of CMC-Na and Glycerin in Tobacco (Nicotiana Tabacum L.) Hand Sanitizer Gel Using the Simplex Lattice Design. Int J Food Agric Nat Resour 2023;4:10–7. https://doi.org/10.46676/ij-fanres.v4i1.108. DOI: https://doi.org/10.46676/ij-fanres.v4i1.108

Astutiningsih C, Kristianti J. The Inhibitor Activity Test of Green Okra Fruit Fraction (Abelmoschus Esculentus) Against Candida Albicans. Sci Midwifery 2022;10:2102–9. https://doi.org/10.35335/midwifery.v10i3.617. DOI: https://doi.org/10.35335/midwifery.v10i3.617

Ardiati KN. Formulasi Sediaan Gel Ekstrak Daun Lidah Mertua (Sansivieria Trifasciata) Dengan Gelling Agent Karbopol-934 dan Uji Aktivitas Antibakteri Secara In Vitro Terhadap Staphylococcus Epidermidis 2018.

Yusuf AL, Nugraha D, Wahlanto P, Indriastuti M, Ismail R, Himah FA. Formulasi Dan Evaluasi Sediaan Gel Ekstrak Buah Pare (Momordica Charantia L.) Dengan Variasi Konsentrasi Carbopol 940. Pharm Genius 2022;1:50–61. DOI: https://doi.org/10.56359/pharmgen.v1i01.149

Lau SHA. Formulasi dan evaluasi kestabilan fisik sediaan gel topikal ekstrak etanol daun ciplukan (Physalis angulata L.) dengan variasi konsentrasi karbopol 940 serta pengujian hedoniknya. J Farm Sandi Karsa 2019;5:120–6. DOI: https://doi.org/10.36060/jfs.v5i2.54

Alatas A, Anindhita MA. Pengaruh carbopol 940 sebagai gelling agent terhadap karakteristik fisikokimia sediaan masker gel peel-off ekstrak kulit buah melon oranye (Cucumis melo L.). BENZENA Pharm Sci J 2023;1. DOI: https://doi.org/10.31941/benzena.v1i2.2326

Hasyim N, Pare KL, Junaid I, Kurniati DA. Formulasi dan Uji Efektivitas Gel Luka Bakar Ekstrak Daun Cocor /bebek (kalanchoe Pinnata L.) pada Kelinci (Oryctolagus Cuniculus) n.d.

Nagajyothi A, Gorantla N, Pavan R, Sreedhar AA. Formulation and Evaluation of Herbal Ointments containing Aqueous Extract of AcalyphaIndica using different types of Ointment Bases. Int J Chem Pharm Sci 2014;2:1276–80.

Rahim F, Aria M, Aji NP. Formulasi Krim Ekstrak Etanol Daun Ubi Jalar (Ipomoeae batatas L.) untuk Pengobatan Luka Bakar. Sci J Farm Dan Kesehat 2015;1:21. https://doi.org/10.36434/scientia.v1i1.12. DOI: https://doi.org/10.36434/scientia.v1i1.12

Siregar, Y.D.I, Utami P. Pemanfaatan ekstrak kulit melinjo merah (Gnetum Gnemon) sebagai pewarna alami pada pembuatan lipstick. J Kim Val 2014;4, 2,:98–108. DOI: https://doi.org/10.15408/jkv.v0i0.3607

Malaha N, Sartika D, Pannyiwi R, Zaenal Z, Zakiah V. Efektifitas Sediaan Biospray Revolutik Terhadap Jumlah Fibroblas Dalam Proses Penyembuhan Luka. SAINTEKES J Sains, Teknol Dan Kesehat 2023;2:161–9. DOI: https://doi.org/10.55681/saintekes.v2i2.70

Primadina N, Basori A, Perdanakusuma DS. Proses penyembuhan luka ditinjau dari aspek mekanisme seluler dan molekuler. Qanun Med J Fac Med Muhammadiyah Surabaya 2019;3:31–43. DOI: https://doi.org/10.30651/jqm.v3i1.2198

Mappa, T., Edy, H. J., & Kojong N. Formulasi Gel Ekstrak Daun Sasaladahan (Peperomia pellucid (L.) H.B.K) dan Uji Efektivitasnya Terhadap Luka Bakar pada Kelinci (Oryctolagus Cuniculus). J Ilm Farm 2, 2, 51 2013.

inawati, C., Agustina, R., & Suhartono E. Penyembuhan Luka dengan penurunan Eritmia pada Tikus Putih (Rattus norvegicu) yang diberikan Getah Batang Jarak Cina (Jatropha multifida L. DK, 3, 1, 1-11 2015.

Syamsuni. farmasetika Dasar dan Hitungan Farmasi. Jakarta : EGC: 2006.

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