Test of anticholesterol activity extract and fractions of clove mistletoe leaves (Dendrophthoe pentandra (L.) Miq.) in vitro
Article metrics
OJS usage and citation sourcesViews and downloads are recorded by OJS; statistics processing may be delayed. A dash means the source count is unavailable. Search Google Scholar
Article Sidebar
Cindhan Bingar Widyaswara, Rahmat Hidayat, Danang Raharjo (2026). Test of anticholesterol activity extract and fractions of clove mistletoe leaves (Dendrophthoe pentandra (L.) Miq.) in vitro. Journal of Pharmaceutical and Sciences, JPS Volume 9 Nomor 4 (2026). https://doi.org/10.36490/journal-jps.com.v9i4.1902
Main Article Content
Abstract
Background: Increased consumption of foods high in saturated fat and cholesterol can lead to hypercholesterolemia. Although statins are effective in treating hypercholesterolemia, their use may cause adverse effects, creating a need for alternative therapies derived from natural products. Clove mistletoe leaves (Dendrophthoe pentandra (L.) Miq.) contain bioactive compounds, particularly flavonoids, which have potential anti-cholesterol activity. Methods: Extraction was carried out using the sonication method with 70% ethanol as the solvent. The extract was fractionated using three solvents: water, ethyl acetate, and n-hexane. Phytochemical screening was performed using qualitative tube tests, followed by an in vitro anticholesterol assay using the Liebermann-Burchard method. The IC50 value was calculated using linear regression analysis of the relationship curve of concentration versus percentage of cholesterol reduction. Result: The results showed that the ethyl acetate fraction had the highest anticholesterol activity with an IC50 value of 18,043 ppm, followed by the water fraction (22,910 ppm), ethanol extract (41,645 ppm), and n-hexane fraction (85,510 ppm). The IC50 value of the ethyl acetate fraction was the closest to the IC50 value of simvastatin (7,703 ppm) as a positive control. Conclusions: The ethyl acetate fraction showed the highest activity with an IC50 value of 18,043 ppm, while the n-hexane fraction showed the lowest activity with an IC50 value of 85,510
Article Details
Section
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
How to Cite
References
[1] Aini, R. N. U. R. (2023). Antioksidan Ekstrak Etanol Dan Infusa Daun Rambutan (Nephelium Lappaceum L.) Dengan Metode Abts. Skripsi. Duta Bangsa Surakarta. Surakarta.
[2] Alya, R., Sholih, M. G., & Fadhilah, N. Y. (2025). Literature Review: Efektivitas Tanaman Herbal Terhadap Penurunan Kadar Kolesterol Pada Penyakit Hiperkolesterolemia. Jurnal Surya Medika (JSM), 11(1), 228-234.
[3] Andriani, S., & Anggraini, D. I. (2023). Uji aktivitas antikolesterol variasi ekstrak etanol sawi pakcoy (Brassica chinensis) secara in vitro. Jurnal Farmasi Sains dan Terapan (Journal of Pharmacy Science and Practice), 10(1), 44-50.
[4] Aprilianti, N. M., Purgiyanti, P., & Barlian, A. A. (2023). Penentuan kadar total fenol fraksi n-heksan, etil asetat, dan air herba pegagan (Centella asiatica (L) Urban). Parapemikir: Jurnal Ilmiah Farmasi, 12(1), 77-85.
[5] Fatimah, O. N. (2025). Analisis Kadar Fenolik, Flavonoid Dan Aktivitas Inhibisi Enzim Α-Amilase Dari Ekstrak Etanol Serta Fraksi Gagang Cengkeh (Syzygium Aromaticum L.). Skripsi. Duta Bangsa Surakarta.
[6] Hasanah, R. (2023). Uji aktivitas ekstrak etil asetat kulit pisang tanduk (Musa acuminata x Musa balbisiana) sebagai antibakteri Propionibacterium acnes. Skripsi. Universitas dr. Soebandi. Jember.
[7] Ilyas, A. N., Rahmawati, R., & Widiastuti, H. (2020). Uji aktivitas antikolesterol ekstrak etanol daun gedi (Abelmoschus manihot (L.) Medik) secara in vitro. Window of Health: Jurnal Kesehatan, 057-064.
[8] Laksono, S., & Afiyani, N. (2023). Farmakoterapi pasien hiperkolesterolemia familial: Suatu tinjauan narasi. Farmasains, 10(1),9-18.
[9] Lindawati, N. Y., Ningsih, D. W., Tinggi, S., Kesehatan, I., & Tengah, J. (2020). Aktivitas Antikolesterol Ekstrak Etanol Buah Kiwi Hijau (Actinidia deliciosa). Ilmiah Manuntung, 6(2), 183-191.
[10] Nafiah, A. (2025). Studi In Vitro Antikolesterol Dan Flavonoid Total Dari Ekstrak Etanol Bawang Putih, Bawang Hitam Dan Bawang Putih Tunggal (Allium Sativum L.). Skripsi. Duta Bangsa Surakarta. Surakarta.
[11] Nola, F., Putri, G. K., Malik, L. H., & Andriani, N. (2021). Isolasi senyawa metabolit sekunder steroid dan terpenoid dari 5 tanaman. Syntax Idea, 3(7), 1612-1619.
[12] Prasetyo, V. (2024). Fraksinasi dan Penetapan Kadar Flavonoid Pada Ekstrak Etanol Selada Air (Nasturtium offcinale W. T. Aiton). Skripsi. Universitas Pakuan. Bogor.
[13] Qhoir, F. (2023). Skrining fitokimia metabolit sekunder ekstrak mahoni (Swietenia mahagony) potensial sebagai medikasi virus covid-19. Skripsi. Universitas Medan Area. Medan.
[14] Sari & Susilowati. (2020). Perbandingan Kadar Flavonoid Total Seduhan Daun Benalu Cengkeh (Dendrophthoe petandra L.) pada Bahan Segar dan Kering. Jurnal Farmasi (Journal of Pharmacy), 9(2), 33-40.
[15] Vifta, R. L., Mafitasari, D., & Rahman, E. (2020). Skrining Antioksidan Dan Aktifitas Antidiabetes Ekstrak Terpurifikasi Etil Asetat Kopi Hijau Arabika (Coffea arabica L.) Secara Spektrofotometri Uv-Vis. Jurnal Zarah, 8(2), 62-68.
[16] Fitrilia, T. (2015). Ekstrak Daun Benalu Cengkeh (Dendrophthoe Pentandra (L.) Miq) Sebagai Agen Antioksidan Dan Antidiabetes Secara In Vitro. Doctoral dissertation. Bogor Agricultural University (IPB). Bandung.
[17] Fitrilia, T. (2017). Inhibisi Enzim α-Glukosidase Menggunakan Ekstrak Daun Benalu Cengkeh (Dendrophthoe pentandra (L.) Mic). Jurnal Agroindustri Halal, 3(1), 041-047.