Indonesia ranks third in the number of tuberculosis, after India and China. Treatment of tuberculosis takes a long time, and the lack of discipline in taking medication has caused the Mycobacterium tuberculosis to be resistant to many synthetic drugs that have been used so far. In contrast, the discovery of new synthetic drugs is very slow. Traditionally, neem bark (Azadirachta indica JUSS.) has been used to treat coughing up blood and phlegm, and the results of previous studies show that ethanol extract of neem bark in vitro can inhibit the growth of Mycobacterium tuberculosis. This study was conducted to determine the toxicity of neem bark extract, formulation of neem bark ethanol extract into tablet preparations, and the potential of this tablet preparation as anti-tuberculosis in vivo in animals. Neem bark extract was made by percolation using an ethanol extractor. Toxicity test was carried out in the form of an acute toxicity test on mice to calculate the LD50 value and histopathological observations of the liver, lungs, and intestines. The tablets were made by wet granulation. The potential test of tablet preparations as anti-tuberculosis was performed in vivo on animals infected with Mycobacterium tuberculosis H37Rv using a nebulizer. The results showed LD 50 = 11.85 ± 0.571, including mild toxic. The ethanolic extract of neem bark can be formulated into tablets using a 6% gelatin binder, Manihot starch and primojel as a binder, and a mixture of starch and lactose as a filler. Neem bark extract tablets can cure tuberculosis of experimental animals that have been infected with Mycobacterium tuberculosis H37RV, which is given a dose of 3 times a day two tablets (50 mg/tablet) for six weeks seen tuberculosis bacteria from +3 to negative, and a dose of 3 times a day once a day. Tablet looks from +3 to +1.
Almas, K. (1999). The Antimicrobial Effects of extracts of Azadirachta indica (Neem) and Salvadora persica (Arak) Chewing Sticks. Indian J Dent Res 10 (1) : 23-6.
Anonymous, 2000, Guidelines for the Implementation of Traditional Medicine Clinical Trials, Ministry of Health RI, Jakarta.
Brooks, GF, Janet SB, Stephen AM (2001). Medical Microbiology. Translator EM, Kuntaman.,
EBW, NMM, SH, Lindawati A. First Edition. Jakarta: Salemba Medika. Pages 317-320.
Dalimartha, Setiawan, (2000), Atlas of Indonesian Medicinal Plants, Volume I, Printing I, Trubus Agriwidya, Jakarta. Das, BK, Mukherjee, SC, Sahu, BB, and Murjani,
G. (1999). Neem (Azadirachta indica) Extract as an Antibacterial Agent Against Fish Pathogenic Bacteria. Indian J Exp Biol 37(11): 1097-100.
Indonesian Ministry of Health, (1995), Materia Medika Indonesia, Volume IV, Ministry of Health of the Republic of Indonesia,
Jakarta. Pages: 297-307,322-339.
Indonesian Ministry of Health, (2000), General standard parameters of medicinal plant extracts, Ministry of Health of the Republic of Indonesia, Jakarta. Hal: 3.
Gagoup, IA and DK Hayes, (1984). Effect of larvae treatment with azadirachtin, a molting
inhibitory component of neem tree, on reproductive capacity of the face fly Musca autumnalis De Geer (Diptera Muscidae), Env. Ento.13
Kardinan, A. and Taryono, (2003). Cancer-fighting medicinal plants. PT. Agromedia Pustaka, Jakarta.
Misnadiarly. (2006). Tuberculosis and Atypical Mycobacteria. Jakarta : PT. Dian Rakyat. Pages : 98-100.
Ruskin, FR, (1993). Neem : a tree for solving global problems. National Academy Press, Washington, DC 141 pp.
Rembold, T., (1989). Azadirachtin, their structure and mode of action. Federal Republic of Germany Press. 15 pp: 1639-1643.
Download data is not yet available.