Salmonella typhosa
in vitro
traditional Chinese medicines
Anti-bacterial activity
Earthworm (Perionyx sp.)

How to Cite

Indriana, M., & salman, salman. (2020). POTENTIAL ANTI-BACTERIAL POWDER BOOTING WATER, n-HEXANA EXTRACT AND EARTHWORM CHLOROFORM EXTRACT (Peryonix sp.) AGAINST Salmonella typhosa BACTERIA. Journal of Pharmaceutical And Sciences, 3(1), 62-68. https://doi.org/10.36490/journal-jps.com.v3i1.89
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Earthworms are animals that have many benefits and are easy to find—results of information from people consume boiled water of earthworms three times a day for 7 days is used as a typhus medicine, other information is obtained from electronic media, many people also consume earthworms as a typhoid medicine in powder form which is filled into capsules Which can be found in Chinese drug stores, but until now scientific research on earthworms (Perionyx sp.) as an anti-bacterial has not been done. Extraction is done by maceration. Multilevel maceration using chloroform and n-hexane as solvent. The class of chemical compounds was determined on the ethyl acetate extract and the n-hexane extract of the earthworm Perionyx sp.), including alkaloids, glycosides, steroids/triterpenoids, flavonoids, saponins, and tannins. Testing of antibacterial activity of chloroform extract and n-hexane extract against Salmonella typhosa was carried out in vitro by agar diffusion method using metal buffer at a concentration of 500 mg/ml; 400 mg/ml; 300 mg/ml; 200 mg/ml; 100 mg/ml and 50 mg/ml. The results of determining the class of chemical compounds from the chloroform extract of earthworms (Perionyx sp.) showed the presence of chemical compounds belonging to the alkaloid group, while the n-hexane extract did not indicate the presence of chemical compounds detected. The n-hexane extract of tar h (Perionyx sp.) had an inhibitory effect on the growth of Salmonella typhosa at a minimum inhibitory concentration of 400 mg/ml of 10.9mm, while the chloroform extract had a minimum inhibitory concentration of 300 mg/ml with an inhibitory diameter of 12.87 mm.



Anonymous. 2008. Salmonella typhosa. http://id. Wikipedia.org/wiki/Salmonella.
Cahyo, Dimas. 2011. Rich from Cultivating Earthworms and Silkworms. Arta Pustaka Publisher.
Ciptanto, Sapto. 2011. Gaining Black Gold Through Cultivation of Earthworms. Yogyakarta. Publisher Lily Publisher.
Indonesian Ministry of Health. 1980. Materia Medika Indonesia. Volume IV. Jakarta.
Indonesian Ministry of Health. 1989. Materia Medika Indonesia. Volume V. Jakarta.
Indonesian Ministry of Health. 1995. Materia Medika Indonesia. Volume Vi. Jakarta.
Directorate General of POM. 1979. Indonesian Pharmacopeia. ill edition. Indonesian Ministry of Health. Jakarta.
Directorate General of POM. 1995. Indonesian Pharmacopeia. Edition IV. Indonesian Ministry of Health. Jakarta.
Directorate General of POM. 2000. General Standard Parameters of Medicinal Plant Extracts. Print I. Ministry of Health of the Republic of Indonesia. Jakarta.
Dwidjoseputro. (1998). Microbiology Fundamentals. Jakarta: Publisher DJBanbatan. Page 38.
Gunawan, IA (2009). Potential of Pare Fruit as Antibacterial Salmonella typhimurium. Faculty of Educational Sciences Teacher Training. Mahasaraswati University, Denpasar.
Harborne, JB 1987. Phytochemical Methods. Translators: Kosasih Padmawinata and Iwang Soediro. Bandung: ITB Publisher.
Hoan, Tan Tjay. & Rahardja, Kirana. 2002. Important Drugs Efficacy, Use, and Side Effects, 2nd edition. Jakarta: Publisher PT. Elex Media Komputindo, Gramedia Group.
Irianto, K. 2006. Microbiology Reveals the World of Microorganisms. Volume I. Bandung: Yrama Widya Publisher.
Jawetz, E. 2001. Medical Microbiology. Translator: Mudihardi, E., et al. Surabaya: Salemba Medika Publisher.
Khairuman, et al. 2010. Making Profits From Raising Worms. Jakarta: PT Agro Media Pustaka.
Khairani, ummah 2010, Examination of Protein Levels in Earthworms Contained in Market Organic Waste by Kjeldahl, Tjut Nyak Dhien University. Medan. Page 49.
Lay., BW (1996), Microbial Analysis in the Laboratory, Jakarta: Raja Grafindo Persada Publisher.
Lee, J. 1983. Microbiology. First Edition. USA: The Barnes and Noble Outline Series.
bead. EW 2011. Anti-bacterial Activity Test of Boiled Water and Ethanol Extract of Earthworm (Megascolex sp.) Against Salmonella typhosa, Escherichia coli, Shigella dysenteriae bacteria. Medan. USU.
Palczar. MJ, et al, (2008), Fundamentals of Microbiology, First Volume, Jakarta: Ul Press. Pratiwi, ST 2008. Pharmaceutical Microbiology. Jakarta: Erllangga
Rasifirtia Publisher, 2011. Determination of Protein Content in Pheretima and Perionyx Seca~ Kjeldahl Earthworms, Medan: UTND.
Robinson, T. 1995. High Organic Content of Plants. Translator: Padma Winata, K. Bandung.ITB.
Rukmana, Rahmat. 1999. Cultivation of Earthworms. Yogyakarta: Kanisius.
Shofyan. M, 2010. Factors Affecting Enzyme Activity.
Suin., NM, 2006. Soil Animal Ecology, Jakarta: Publisher Earth Literacy. Case. 94.
Sumardi, 1998. Detection and Characterization of Antibiotic Compounds from Extracts and Isolates of Microbes in the Body of the Earthworm Allolobophora rosea. Bogor. Bogor Agricultural Institute.
Yuli Priyanto, H. 2010. Soil Biology and Management Strategy. Yogyakarta: Graha science.
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