DETERMINATION OF S-ALLYL CYSTEINE (SAC) COMPOUNDS IN SOLO BLACK GARLIC EXTRACT BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY
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Romsiah Romsiah, Jeshi Gecka Ayu, Ahmad Fatoni (2022). DETERMINATION OF S-ALLYL CYSTEINE (SAC) COMPOUNDS IN SOLO BLACK GARLIC EXTRACT BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY. Journal of Pharmaceutical and Sciences, Vol. 5 No. 1 (2022): JPS Volume 5 Nomor 1 (2022). https://doi.org/10.36490/journal-jps.com.v5i1.101
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Abstract
The research has been regarding of method validation and determination S-allyl cisteine coumpound from solo black garlic extract by high perfomance liquid chromatography. The stationary phase used is ODS C-18 and the mobile phase is acetonitril : aquabidest (50:50 v/v) at a flow rate of 1,0 ml/min and detected at wavelength 195 nm for S-allyl cysteine. Before determination should be qualitative test determinant a maximum wavelength of sample. Standard solution S-allyl cysteine (SAC) with concentration 50, 100, 150, and 200 ppm wich is measured at a maximum wavelength of 195 nm, the value of regression line equation is y = 4,818x + 46,5 with the value of the correlation coefficient is (r) = 0,998. Obtained result sample wavelength 194 nm near to maximum wavelength of standard solution. The result of determination solo black garlic extract show for solo black garlic extract contain S-allyl cysteine 52,055 % with category high antioxidant activity 50-90 %. Based on the result it can be concluded that a HPLC is valid and the sample solo black garlic still show high S-allyl cysteine coumpound.
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Aini, S. Q. dan Shovitri, M. (2018). Studi awal pemanfaatan bawang putih yang dihitamkan sebagai antibakteri. Jurnal Sains dan Seni ITS, 7(1), 9-12.
Bae, S. E., Cho, S. Y., Won, Y. D., Lee, S. H., dan Park, H. J. (2012). A comparative study of the different analytical methods for analysis of S-allyl cysteine in black garlic by HPLC. Journal Food Science and Technology, 46, 532-535. doi: 10.1016/ j.lwt.2011.11.013.
Bharat, P., Dave, A. R., Chandola, H. M., Goyal, M. R., Shukla, V. J., dan Khant, D. B. (2014). Comparative analytical study of single bulb and multi bulb garlic (Allium sativum Linn.). International ]mJournal of Alternative Medicine, 2(4), 87-91.
Departemen Kesehatan Republik Indonesia. (1995). Farmakope indonesia (Edisi IV). Jakarta : Departemen Kesehatan.
Hernawan, U. K. dan Setyawan, A.D. (2003). Senyawa organosulfur bawang putih (Allium sativum L.) dan aktivitas biologinya. Jurnal Biofarmasi, 1(2), 65-76.
Putri, P. E. (2019). Perbandingan aktivitas antioksidan ekstrak bawang putih dan bawang putih tunggal bentuk segar dan fermentasi dengan metode DPPH. Skripsi, Palembang : Program Sarjana Farmasi Sekolah Tinggi Ilmu Farmasi Bhakti Pertiwi.
Rohman, A. (2009). Kromatografi untuk analisa obat. Yogyakarta :
Graha Ilmu.
Saefudin., Marusin, S., dan Chairul. (2013). Aktivitas antioksidan pada enam jenis tumbuhan Sterculiaceae. JurnalPenelitian Hasil Hutan, 31(2), 103-109.
Sari, F. D. P. dan Utami, P. I. (2009). Penetapan kadar kloramfenikol dalam tetes mata pada sediaan generic dan merk dengan metode kromatografi cair kinerja tinggi. Journal pharmacy, 6(2), 53-59.
Yoo, M., Lee, S., Lee, S., Seog, H., dan Shin, D. (2010). Validation of high perfomance liquid chromatoghraphy methods for determination of bioactive sulfur compounds in garlic bulbs. Journal Food Science and Biotechnology, 19, 1619-1626. doi: 10.1007/s10068-010-0229-1.