Phytochemical Study and Total Phenolic Content Analysis of Methanol Extract of Stem Bark of Merawan (Hopea mengarawan Miq.)
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Abstract
Dipterocarpaceae is a family of plants that has good wood quality and durability. Apart from that, wood for this family is known to contain the compound resveratrol which has bioactivity, such as anti-cancer and anti-inflammatory. Several genus and species of this family are threatened with extinction. Merawan (Hopea mengarawan Miq.) is one of extinct species from Dipterocarpaceae which according to several research contains secondary metabolite compounds. The objective of this research was to determine the content of secondary metabolite compounds and determine the total phenolic content of merawan. The maceration method was used to extract the stem bark of H. mengarawan Miq., then continued with phitochemical screening, and total phenolic content analysis. Phytochemical screening was carried out to identify secondary metabolite compounds contained in the methanol extract of H. mengarawan Miq. stem bark qualittatively. Determination of total phenolic content was carried out using the colorimetric method with Folin-Ciocalteu reagent and gallic acid as a comparison standard. The result of this study indicate that the methanol extract of stem bark of H. mengarawan Miq. contains secondary metabolite compounds, such as saponins, steroids, tannins, alkaloids, flavonoids, and phenolic with the total phenolic content of 13,518±0,228 mg GAE/g.
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References
Petrus S, Manurung TF, Kartikawati SM. Identifikasi Jenis Pohon Famili Dipterocarpaceae pada Hutan Rawa Gambut di KHDTK Universitas Tanjungpura Kecamatan Mandor Kabupaten Landak Kalimantan Barat. Jurnal Hutan Lestari 2021;9:584-598. https://dx.doi.org/10.26418/jhl.v9i4.49103 DOI: https://doi.org/10.26418/jhl.v9i4.49103
Suryo R, Martunis, Harnelly E. Keanekaragaman dan Status Konservasi Meranti Putih (Parashorea lucida Kurz) pada Kawasan Jambur Gele, Taman Nasional Gunung Leuser. Jurnal Ilmiah Mahasiswa Pertanian 2023;8:617-626. https://doi.org/10.17969/jimfp.v8i3.24457
Kusmana C, Lathifah A. Keragaan Tegakan Merawan (Hopea mengarawan Miq.) dan keruing gunung (Dipterocarpus retusus blume) di Hutan Penelitian Dramaga, Bogor. Jurnal Silvikultur Tropika 2021;12:186-193. http://dx.doi.org/10.29244/j-siltrop.12.3.186-193 DOI: https://doi.org/10.29244/j-siltrop.12.3.186-193
Fikayuniar L, Zulfa AN, Nurlelah N, Nurjanah A., Nissa K, Haniatin K, Andriyani N. A Review: Penapisan Fitokimia Simplisia Bunga Telang Untuk Identifikasi Golongan Senyawa Metabolit Sekunder. Jurnal Kesehatan Tambusai 2024;5:2081-2087. https://doi.org/10.31004/jkt.v5i1.24043
Atun S. Aktivitas Oligoresveratrol dari Kulit Batang Hopea mengarawan (Dipterocarpaceae) sebagai Penangkap Radikal Hidroksil. HAYATI Journal of Biosciences 2006;13:65-68. https://doi.org/10.4308/hjb.13.2.65 DOI: https://doi.org/10.1016/S1978-3019(16)30383-7
Supriningrum R, Nurhasnawati H, Faisah S. Penetapan Kadar Fenolik Total Ekstrak Etanol Daun Serunai (Chromolaena Odorata L.) dengan Metode Spektrofotometri Uv-Vis. Al Ulum: Jurnal Sains dan Teknologi 2020;5:54-57. http://dx.doi.org/10.31602/ajst.v5i2.2802 DOI: https://doi.org/10.31602/ajst.v5i2.2802
Oktavia FD, Sutoyo S. Skrining Fitokimia, Kandungan Flavonoid Total, dan Aktivitas Antioksidan Ekstrak Etanol Tumbuhan Selaginella doederleinii. Jurnal Kimia Riset 2021;6:141-153. http://dx.doi.org/10.20473/jkr.v6i2.30904 DOI: https://doi.org/10.20473/jkr.v6i2.30904
Alfiani LA. Uji Aktivitas Penghambatan Enzim α–amilase oleh Ekstrak Herba Ciplukan (Physalis Angulate L) Secara In Vitro. Jurnal Ilmiah Wahana Pendidikan 2022;8:335-346. https://doi.org/10.5281/zenodo.7049485
Putri NM, Wiraningtyas A, Mutmainah PA. Perbandingan Metode Ekstraksi Senyawa Aktif Daun Kelor (Moringa Oleifera): Metode Maserasi dan Microwave-Assisted Extraction (MAE). Dalton : Jurnal Pendidikan Kimia dan Ilmu Kimia 2021;4:25-33. http://dx.doi.org/10.31602/dl.v4i2.5931 DOI: https://doi.org/10.31602/dl.v4i2.5931
Lulan TYK, Fatmawati S, Santoso M, Ersam T. α-Viniferin as a Potential Antidiabetic and Antiplasmodial Extracted from Dipterocarpus littoralis. Heliyon 2020;6:1-6. https://doi.org/10.1016/j.heliyon.2020.e04102 DOI: https://doi.org/10.1016/j.heliyon.2020.e04102
Sinulingga S, Subandrate, Safiyudin. Uji Fitokimia dan Potensi Antidiabetes Fraksi Etanol Air Benalu Kersen (Dendrophtoe petandra (L) Miq). Jurnal Kedokteran Dan Kesehatan 2020; 16:76-83. https://doi.org/10.24853/jkk.16.1.76-83 DOI: https://doi.org/10.24853/jkk.16.1.76-83
Abdulrahman, Utami SR, Widia, Roanisca O. Kajian Metabolit Sekunder Batang Bajakah (Spatholobus littoralis hassk.) dalam Pengembangan sebagai Obat Herbal Antikanker Payudara dan Antioksidan. Seminar Nasional Penelitian dan Pengabdian pada Masyarakat; 29-30 September 2021; Pangkalpinang, Indonesia: Universitas Bangka Belitung; 2021. p. 46-49. https://doi.org/10.33019/snppm.v5i0.2689
Andriani D, Murtisiwi L. Penetapan Kadar Fenolik Total Ekstrak Etanol Bunga Telang (Clitoria ternatea L.) Dengan Metode Spektrofotometri UV Vis. Cendekia Journal of Farmasi 2018. 2:32-38. https://doi.org/10.31596/cjp.v2i1.15 DOI: https://doi.org/10.31596/cjp.v2i1.15
Mukhriani, Sugiarna R, Farhan N, Rusdi M, Ikhlas AM. Kadar Fenolik dan Flavonoid Kadar Fenolik dan Flavonoid Total Ekstrak Etanol Daun Anggur (Vitis vinifera L). Journal of Pharmaceutical and Sciences 2019;2:95-102. https://doi.org/10.24252/djps.v2i2.11503 DOI: https://doi.org/10.24252/djps.v2i2.11503
Dewantara LAR, Ananto AD, Andayani Y. Penetapan Kadar Fenolik Total Ekstrak Kacang Panjang (Vigna unguiculata) dengan Metode Spektrofotometri UV-Visible. Lumbung Farmasi: Jurnal Ilmu Kefarmasian 2021;2:13-19. https://doi.org/10.31764/lf.v2i1.3759 DOI: https://doi.org/10.31764/lf.v2i1.3759
Sulasmi ES, Saptasari M, Mawaddah K, Zulfia FA. Tannin Identification Of 4 Species Pterydophyta From bluran National Park. Journal Of Physics Conference Series 2019;1241:1-7. http://dx.doi.org/10.1088/1742-6596/1241/1/012002 DOI: https://doi.org/10.1088/1742-6596/1241/1/012002
Harahap SN, Situmorang N. Skrining Fitokimia dari Senyawa Metabolit Sekunder Buah Jambu Biji Merah (Psidium guajava L.). EduMatSains: Jurnal Pendidikan, Matematika, dan Sains 2021;5:153-164. https://doi.org/10.33541/edumatsains.v5i2.2204 DOI: https://doi.org/10.33541/edumatsains.v5i2.2204
Kumalasari E, Mudjib NN. Musiam S. Penetapan Kadar Fenolik Total Ekstrak Etanol 70% dan Fraksi Etil Asetat Daun Bawang Dayak (Eleutherine palmifolia (L.) Merr) dengan Metode Spektrofotometri UV Vis. Jurnal Insan Farmasi Indonesia 2021;4:74-84. https://doi.org/10.36387/jifi.v4i1.665 DOI: https://doi.org/10.36387/jifi.v4i1.665
Feninlambir ML, Rawar EA, Yuhara NA, Jalan KI, Kim S. Aktivitas Antioksidan dan Kadar Total Fenolik Dalam Minyak Atsiri Biji Pala (Myristica fragrans Houtt). Jurnal Penelitian Farmasi Indonesia 2023;12:111-116. https://doi.org/10.51887/jpfi.v12i2.1807 DOI: https://doi.org/10.51887/jpfi.v12i2.1807
Khadijah, Jayali AM, Umar S, Sasmita I. Penentuan Total Fenolik Dan Aktivitas Antioksidan Ekstrak Etanolik Daun Samama (Anthocephalus macrophylus) Asal Ternate, Maluku Utara. Jurnal Kimia Mulawarman 2017; 15:11-18. http://dx.doi.org/10.30872/jkm.v15i1.495 DOI: https://doi.org/10.30872/jkm.v15i1.495
Zahra NN, Muliasari H, Andayani Y, Sudarma IM. Analisis Kadar Fenolik Total Dan Aktivitas Antiradikal. Analit: Analitical and Environmental Chemistry 2021;6:74-82. http://dx.doi.org/10.23960/aec.v6.i1.2021.p74-82 DOI: https://doi.org/10.23960/aec.v6.i1.2021.p74-82
Candra LMM, Andayani Y, Wirasisya DG. Pengaruh Metode Ekstraksi Terhadap Kandungan Fenolik Total dan Flavonoid Total Pada Ekstrak Etanol Buncis (Phaseolus vulgaris L). Jurnal Pijar Mipa 2021;16: 397–405. https://doi.org/10.29303/jpm.v16i3.2308 DOI: https://doi.org/10.29303/jpm.v16i3.2308
Munteanu IG, Apetrei C. Analitical Methods Used in Determining Antioxidant Activity: A Riview. International Journal of Molecular Sciencies 2021;22:1-30. https://doi.org/10.3390/ijms22073380 DOI: https://doi.org/10.3390/ijms22073380
Pérez M, Dominguez-López I, Lamuela-Raventós RM. The Chemistry Behind the Folin−Ciocalteu Method for the Estimation of (Poly)phenol Content in Food: Total Phenolic Intake in a Mediterranean Dietary Pattern. Journal of Agricultural and Food Chemistry 2023;7:17543–17553. https://doi.org/10.1021/acs.jafc.3c04022 DOI: https://doi.org/10.1021/acs.jafc.3c04022
Ayyun K, Rosydah YKI, Atikah N, Arianti SP, Maulidini C, Agustino F, Putri NK, Seran AA, Klau SIC, Ningsih AW. Artikel Review: Profil Studi Fitokimia dan Aktivitas Farmakologi Buah Mangga (Mangifera indica L.). Journal Sains Farmasi dan Kesehatan 2023;1:60-68. https://doi.org/10.62379/jfkes.v1i2.346 DOI: https://doi.org/10.62379/jfkes.v1i2.346