Identification of Active Compounds in Dadap Serep Leaves (Erythrina subumbrans (Hassk.) Merr) and Their Potential as Antioxidants
Article Sidebar
Main Article Content
Page: 60-67
Abstract
Background: Erythrina subumbrans (Hassk.) Merr, locally known as dadap serep, is a medicinal plant traditionally used to treat various ailments, suggesting the presence of bioactive compounds. Scientific exploration of its phytochemical profile and antioxidant potential, however, remains limited compared to other species in the genus. Objective: This study aimed to (1) identify the bioactive compound groups in the 70% ethanol extract of Erythrina subumbrans leaves through phytochemical screening and Thin Layer Chromatography (TLC), and (2) evaluate its antioxidant activity quantitatively using the DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging method. Methods: The leaves were extracted via maceration using 70% ethanol. The extract was subjected to standard phytochemical screening tests for flavonoids, alkaloids, saponins, and tannins. Flavonoid profiling was performed using TLC with a quercetin standard. Antioxidant activity was assessed by the DPPH method, with vitamin C as a reference standard, and the IC₅₀ value was determined. Results: Phytochemical screening confirmed the presence of flavonoids, alkaloids, saponins, and tannins. TLC analysis indicated the presence of flavonoid compounds, with one major spot showing an Rf value similar to the quercetin standard. The DPPH assay revealed that the extract possesses strong antioxidant activity, with an IC₅₀ value of 49.94 ppm. According to established antioxidant strength criteria, this IC₅₀ value is categorized as very strong.Conclusion: The 70% ethanol extract of Erythrina subumbrans leaves contains diverse bioactive compounds, particularly flavonoids, and exhibits very strong antioxidant activity. These findings scientifically support its traditional use and highlight its potential as a promising source of natural antioxidants for further pharmaceutical or nutraceutical development.
Downloads
Article Details
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
References
A. Iga Maharani et al., “Prosiding SEMNAS BIO 2021 Peran Antioksidan Alami Berbahan Dasar Pangan Lokal dalam Mencegah Efek Radikal Bebas,” Prosiding SEMNAS BIO 2021, 2021.
J. Medfarm, F. dan Kesehatan, U. Nur Ma, C. Ayu Saputri, A. Sunan Giri Ponorogo, and J. Batoro Katong, “Formulasi Dan Karakterisasi Hidrogel Ekstrak Daun Dadap Serep (Erythrina folium) Dalam Bentuk Plester Sebagai Penurun Demam,” vol. 8, no. 1, pp. 8–14, 2019. DOI: https://doi.org/10.48191/medfarm.v8i1.11
P. A. Nasution, R. Batubara, and Surjanto, “Tingkat Kekuatan Antioksidan Dan Masyarakat Terhadap Teh Daun Gaharu (Aquilaria malaccensis Lamk) Berdasarkan Pohon Induksi dan Non-Induksi,” 2015.
N. Y. Lindawati, S. Hudzaifah Ma’ruf, S. Tinggi, I. Kesehatan, and N. Surakarta, “Penetapan Kadar Total Flavonoid Ekstrak Etanol Kacang Merah (Phaseolus vulgaris L.) Dengan Metode Kompleks Kolometri Secara Spektrofotometri Visibel,” vol. 6, no. 1, pp. 83–91, 2020. DOI: https://doi.org/10.51352/jim.v6i1.312
E. N. Qomaliyah, N. Indriani, A. Rohma, and R. Islamiyati, “Skrining Fitokimia, Kadar Total Flavonoid dan Antioksidan Daun Cocor Bebek Phytochemical Screening, Total Flavonoids and Antioxidants of Kalanchoe Pinnata Linn. Leaves,” Curr. Biochem. 2023, vol. 10, no. 1, 2023. DOI: https://doi.org/10.29244/cb.10.1.1
R. Asra, M. Amrul, and S. Tinggi Ilmu Farmasi Padang, “Evaluasi Penggunaan Kromatografi Lapis Tipis Kinerja Tinggi (KLTKT) Densitometri Silika Gel 60 F 254 Pada Penetapan Kadar Vitamin C Yang Terdapat Pada Daging BUah Naga Ungu (Hylocereus polyrhizus),” 2017.
E. Prasetyo et al., “Uji Aktivitas Antioksidan Menggunakan Metode DPPH (2,2-difenil-1-pikrilhidrazil) Terhadap Ekstrak Etanol Kulit Buah Durian (Durio zibethinnus L.) dari Desa Alasmalang Kabupaten Banyumas,” 2021. [Online]. Available: https://ppjp.ulm.ac.id/journal/index.php/pharmascience DOI: https://doi.org/10.20527/jps.v8i1.9200
A. N. Panaungi, “Uji Daya Hambat Ekstrak Etanol Daun Singkong (Manihot utilissima Pohl) Yang Berasal Dari Kabupaten Pangkajene Sidrap Terhadap Pertumbuhan Bakteri Staphylococcus aureus,” 2021. DOI: https://doi.org/10.35892/jpsht.v6i1.507
’Mihra, M. R. ’Jura, and P. ’Ningsih, “Analisis Kadar Tanin dalam Ekstrak Daun Mimba (Azadirachta indica a. Juss) dengan Pelarut Air dan Etanol,” Akademika Kimia, vol. 7, no. 4, Nov. 2018. DOI: https://doi.org/10.22487/j24775185.2018.v7.i4.11941
D. Załuski, Ł. Cieśla, and Z. Janeczko, “Chapter 7 - The Structure Activity Relationships of Plant Secondary Metabolites with Antimicrobial, Free Radical Scavenging and Inhibitory Activity toward Selected Enzymes,” vol. 45, Atta-ur-Rahman, Ed., in Studies in Natural Products Chemistry, vol. 45., Elsevier, 2015, pp. 217–249. doi: https://doi.org/10.1016/B978-0-444-63473-3.00007-1. DOI: https://doi.org/10.1016/B978-0-444-63473-3.00007-1
Y. P. ’Timilsena, A. ’Phosanam, and R. ’Stockmann, “Perspectives on Saponins: Food Functionality and Applications,” International Journal of Moleculer Scienses, vol. 24, no. 17, Aug. 2023. DOI: https://doi.org/10.3390/ijms241713538
B. E. Andriyanto, P. Ardiningsih, and N. Idiawati, “Skrining Fitokimia Ekstrak Daun Belimbing Hutan (Baccaurea angulata Merr.),” JKK, vol. 5 (4), 2016.
Direktur Jendral Kefarmasian Dan Alat Kesehatan, Supleman I Farmakope Herbal Indonesia Edisi II 2022 Kementerian Kesehatan Republik Indonesia 615.1 Ind f, II. Jakarta, 2022.
N. Fajriani, H. Kurniawan, and F. Nugraha, “Identify the Rhodamin B on lipsticks in the market Using Thin Layer Chromatography (TLC) Method,” Journal Syifa Sciences and Clinical Research, vol. 4, no. 3, Mar. 2023, doi: 10.37311/jsscr.v4i3.15392. DOI: https://doi.org/10.37311/jsscr.v4i3.15392
A. I. H. Purwani, F. Sari, M. D. Ramadhan, and H. Mawardika, “Aktivitas Antioksidan Ekstrak Daun Beluntas (Pluchea indica L.) Etanol 70% Dab Etil Asetat Menggunakan DPPH,” Jurnal Ilmiah Manuntung, 2024, [Online]. Available: https://api.semanticscholar.org/CorpusID:274241482 DOI: https://doi.org/10.51352/jim.v10i1.740
H. Sepriyani et al., “Aktivitas Antioksidan Ekstrak Metanol Daun Pepaya (Carica papaya L) Dengan Metode 2,2 Dipenhyl-1-Picrylhidrazyl (DPPH),” Jurnal Penelitian Farmasi Indonesia, vol. 9, no. 1, p. 2020, Jun. 2020. DOI: https://doi.org/10.51887/jpfi.v9i1.789
Carr, A., & Frei, B. (1999). Does vitamin C act as a pro-oxidant under physiological conditions? The FASEB Journal, 13(9), 1007-1024. DOI: https://doi.org/10.1096/fasebj.13.9.1007
Sapitri Pangestu, Nia. 2017. “Aktivitas Antioksidan Dan Antibakteri Ekstrak Daun Jatropha Gossypifolia L.” ALOTROP Jurnal Pendidikan dan Ilmu Kimia 1(1): 15–19. DOI: https://doi.org/10.33369/atp.v1i1.2707