Evaluasi Aktivitas Penghambatan α-Amilase, α-Glukosidase, dan Aktivitas Antioksidan secara In Vitro dari Ekstrak Etanol Lima Sayuran Indonesia: Pendekatan Multivariat dan Korelasi
Statistik artikel
Data OJS dan sumber sitasiTayangan dan unduhan adalah aktivitas yang tercatat di OJS; pemrosesan statistik dapat tertunda. Tanda — berarti angka dari sumber tersebut belum tersedia. Cari di Google Scholar
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Nofrizal, Fatma Haspa Anzalmi, Lusia Eka Putri (2026). Evaluasi Aktivitas Penghambatan α-Amilase, α-Glukosidase, dan Aktivitas Antioksidan secara In Vitro dari Ekstrak Etanol Lima Sayuran Indonesia: Pendekatan Multivariat dan Korelasi. Journal of Pharmaceutical and Sciences, JPS Volume 9 Nomor 3 (2026). https://doi.org/10.36490/journal-jps.com.v9i3.1806
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Hiperglikemia postprandial dan stres oksidatif yang menyertainya diakui sebagai patofisiologi krusial yang memicu progresivitas diabetes melitus tipe 2 beserta komplikasi vaskularnya. Penelitian ini bertujuan untuk mengevaluasi dan membandingkan aktivitas penghambatan enzim α-amilase, α-glukosidase secara in vitro, beserta kapasitas penangkapan radikal bebas, dari ekstrak etanol lima jenis sayuran segar yang umum dikonsumsi di Indonesia: pucuk daun pepaya (Carica papaya), buah pare (Momordica charantia), rebung (Gigantochloa atter), daun, batang muda pakis (Diplazium esculentum), dan buah oyong (Luffa acutangula) Pengujian bioaktivitas dilakukan menggunakan metode kompleks pati-iodin, p-nitrofenil-α-D-glukopiranosida, dan 2,2-difenil-1-pikrilhidrazil (DPPH). Hasil eksperimental menunjukkan bahwa ekstrak D. esculentum (pucuk pakis) memperlihatkan efikasi penghambatan ganda paling baik terhadap α-amilase (IC50 138.50 µg/mL) dan α-glukosidase (IC50 38.20 µg/mL). Selain itu, ekstrak D. esculentum dan C. papaya menunjukkan kapasitas antioksidan lebih baik di antara seluruh sampel yang diuji. Analisis Principal Component Analysis (PCA) dan Pearson correlation mengonfirmasi adanya sinergi positif yang signifikan antara kapasitas reduksi radikal bebas dan inhibisi enzim pencernaan karbohidrat. Kesimpulannya, D. esculentum teridentifikasi sebagai kandidat sayuran fungsional utama yang memiliki properti terapeutik aksi ganda yang baik untuk meredam hiperglikemia postprandial dan menekan stres oksidatif.
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