Isolation and Antibacterial Activity Test of The Extract Ethyl Acetate of Endophytic Fungi from Kencur (Kaempferia Galanga L.)
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Keywords

Kaempferia galanga L
Endophytic fungi
antibacterial activity

How to Cite

Efendi, M. R., Rusdi, M., & Anisa, F. (2020). Isolation and Antibacterial Activity Test of The Extract Ethyl Acetate of Endophytic Fungi from Kencur (Kaempferia Galanga L.). Journal of Pharmaceutical And Sciences, 3(2), 85-92. https://doi.org/10.36490/journal-jps.com.v3i2.42
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Abstract

The aim of this research was to isolate the endophytic fungi found in leaves and rhizomes of Kaempferia galanga L. and to test its antibacterial activity. Isolation of endophytic fungi is carried out by cultivating and sub-cultivating fungi that found in leaves and rhizomes of Kaempferia galanga L.  Identification of endophytic fungi is done by its morphology macroscopically and microscopically. Antibacterial activity testing is carried out  by using the agar diffusion method. From the leaves and rhizome of Kaempferia galanga L., five isolates of endophytic fungi were identified as Torulla sp. (KG001), Mucor sp. (KG002), Fusarium sp. (KG003), Geotricum sp. (KG004), and Drechslera sp. (KG005). Three ethyl acetate extracts of the endophytic fungi Torulla sp. (KG001), Fusarium sp. (KG003), and Drechcera sp. (KG005) had antibacterial activity against Gram positive (Staphylococcus aureus and Vibrio cholera) and Gram negative bacterias (Bacillus subtilis and Eschericia coli)  at concentration of 3.75%. The ethyl acetate extract endophytic fungi that had strong antibacterial activity (10-20 mm in diameter) were Torulla sp. (KG001) and Drechslera sp. (KG005). Meanwhile, the endophytic fungi that had moderate antibacterial activity (inhibition diameter 5-10 mm) was the ethyl acetate extract Fusarium sp. (KG003).The strongest antibacterial activity was shown by the ethyl acetate extract Drechslera Sp. with an inhibitory diameter 16 mm against Vibrio cholera .

https://doi.org/10.36490/journal-jps.com.v3i2.42
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