Screening of Antibacterial Activity of Extract And Fraction of Flower Petals Mussaenda Frondosa L
PDF (Bahasa Indonesia)

Keywords

Mussaenda frondosa L
agar diffusion method
antibacterial

How to Cite

Efendi, M. R. (2019). Screening of Antibacterial Activity of Extract And Fraction of Flower Petals Mussaenda Frondosa L. Journal of Pharmaceutical And Sciences, 2(1), 38-44. https://doi.org/10.36490/journal-jps.com.v2i1.18
Abstract viewed = 703 times
PDF (Bahasa Indonesia) downloaded = 391 times

Abstract

Background: Antibiotics are the most effective drugs against microbial infections, but slowly lose their efficacy to microorganisms due to resistance. So, nowadays, research related to finding new compounds from nature to treat infectious diseases has become important. Mussaenda frondosa L. is a tropical plant native to Indonesia. Traditionally, Mussaenda frondosa L. has been used for the treatment of various diseases, such as headaches and jaundice. The literature study of the antibacterial activity of Mussaenda frondosa L. is still very limited, so research needs to be done regarding the antibacterial activity.

Objective: to determine whether there is antibacterial activity from Mussaenda frondosa L. petal fraction. And whether there is significant antibacterial activity when it is compared to chloramphenicol as positive control antibiotic.

Method: The methodology of this research starts with sampling, sample identification, extraction, and fractionation. Screening for antibacterial activity was carried out by the agar diffusion method.

Results: The most potential antibacterial activity was ethyl acetate fraction with an inhibitory diameter of 5.6 - 8.3 mm against 12 test pathogenic bacteria, except Streptococcus mutans ATCC 25175. The n-hexane fraction showed antibacterial activity against Staphylococcus aureus ATCC 25923, Pseudomonas aeruginosa ATCC 27853 and Vibrio cholerae inaba with an inhibitory diameter of 6.0 - 7.5 mm. Meanwhile, the n-butanol fraction has only antibacterial activity against Staphylococcus aureus ATCC 25923 with a 7.5 mm inhibition diameter.

Conclusion: the most potential fraction that had antibacterial activity was ethyl acetate fraction, by inhibiting 12 test pathogenic bacterias, followed by n-hexane fraction which inhibits 3 bacterias.

https://doi.org/10.36490/journal-jps.com.v2i1.18
PDF (Bahasa Indonesia)

References

Ahmad I, Beg AZ. (2001). Antimicrobial and phytochemical studies on 45 Indian medicinal plants against multi-drug resistant human pathogens. J Ethnopharmacol. 74(2): 113–123.

Aydin, S., A Ciltas, H. Yetim, and I. Akyurt. (2005). Clinical, Pathology and Haematological Effect of Micrococcus luteus in Rainbow Trout (Oncorhyncus mykiss Walbaum). J. Animal and Veterinary Advances. 4 (2): 167-174.

Betnina, V. (1973). Bioautography in Paper and Thin Layer Chromatography and Its Scope in The Antibiotic Field. J. Chromatography (78) : 31-34.

Bhatia, A. and Zahoor, S. (2007). Staphylococcus aureus Enterotoxins: A Review. J. Clinical and Diagnostic Research. (2): 188-197.

Brooks G.F., Butel J.S., Morse S.A. (2001). Medical Microbiology. 22nd ed. USA: Appleton & Lange. 225 – 227.

Brooks, F.G., Bulel, S.J., Merse, A.S. (2005). Mikrobiologi Kedokteran. Jakarta : Salemba Medika.

Dharmojono. (2001). Lima belas Penyakit Menular dari Binatang ke Manusia. Jakarta : Milenia Populer.

Djide, N., Sartini, Kadir, S. (2006). Analisis Mikrobiologi Farmasi. Makassar : Laboratorium Mikrobiologi Farmasi F-MIPA UNHAS.

Ferrari PHP, Cai S, Bombama AC. (2005). Effect of Endodontic Procedures on Enterococci, Enteric Bacteria and Yeast in Primary Endodontic Infections. J. International Endodontic. (38): 372 – 80.

Gomes NV, Filho EDG, Gomes. (2005). Recovery of Enterococcus faecalis after single or multiple visit root canal treatments carried out in infected teeth ex vivo. J. International Endodontic. (38): 697 – 704.

Gopalakrishnan, S. & Vadivel, E. (2010). GC-MS Analysis of some bioactive constituents of Mussaenda frondosa linn. International Journal Of Pharma And BioSciences, 2, 1.

Grosvenor, P. W., Supriono, A., & Gray, D. O. (1995). Medicinal plants from Riau Province, Sumatra, Indonesia. Part 2: antibacterial and antifungal activity. Journal of Ethnopharmacology, 45, 97-111.

Hemaiswarya S, Kruthiventi AK, Doble M. (2008). Synergism between natural products and antibiotics against infectious diseases. Phytomedicine. 15(8): 639–652.

Huh AJ, Kwon YJ. ( 2011)“Nanoantibiotics”: A new paradigm for treating infectious diseases using nanomaterials in the antibiotics resistant era. J Control Release. 156(2): 128–145.

Jawetz, E., Melnick, J.L., Adelberg, E.A., Brooks, G. F., Butel, J.S., Ornston, L. N. (2001). Review of medical microbiology ed. 10. San Francisco: University of California.

Jawetz. J.E. (1992). Mikrobiologi untuk Profesi Kesehatan, Ed. 16. University of California, San Francisco.

Kayser, F.H., Bienz, K.A., Eckert, J., Zinkernagel, R.M. (2005). Medical Microbiology. Germany : Georg Thieme Verlag.

Kim J.E., H.E Kim, J.K Kwang, H.J Lee, H.K Kwon, B.I Kim. (2008). Antibacterial Characteristic of Curcuma xanthorrhiza Extract on Streptococcus mutans. J. Microbiology; 46(2): 228-232.

Montville, T.J., and Matthews, K.R. (2008). Food Microbiology: An introduction. Washington D.C: ASM Press.

Munuswamy H, Thirunavukkarasu T, Rajamani S, Elumalai EK, Ernest D. (2000)A review on antimicrobial efficacy of some traditional medicinal plants in Tamilnadu. J Acute Dis. 2(2), 99–105.

Nikham. (2006). Kepekaan Staphylococcus aureus, Staphylococcus epidermidis dan Pseudomonas aeruginosa terhadap Ekstrak Daun Legundi (Vitex trifolia Linn.) Iradiasi. Jakarta : Batan.

Nychas, G.J.E. and Tassou, C.C. (1999). Preservatives: Traditional Preservatives-Oiland Spices In Encyclopedia Of Food Microbiology. London : Academica Press.

Putra, D.P, Fatra, H.A., & Bakhtiar, A. (2010). Isolasi senyawa antioksidan dari kelopak bunga nusa indah (Mussaeda frondosa L.). Jurnal Farmasi Indonesia, 5, 1.

Reeves, D.S., Phillips, I., Williams, J.D., Wise, R. (1978). Laboratory Methods in Antimicrobial Chemotherapy. New York: Churchill Livingstone.

Syafni, N., Putra, D.P., Arbain, D. (2012). 3,4-Dihygroxybenzoicacid and 3,4-dihhidrobenzaldehyde from the fern Trichomanes chinense L; Isolation, antimicrobial and antioxidant properties. Indo. J. Chem.12(3). 273-278.

Vidyalakshmi, K.S., Hannah, R., Vasanthi, H.R., Rajamanickam, G.V. (2008). Ethnobotany, phytochemistry and pharmacology of Mussaenda Species (Rubiaceae). Ethnobotanical Leaflets, 12, 469-475.

Zuorro, A., Fidaleo, M., Lavecchia, R. 2010. Solubility Enhancement and Antibacterial Activity of Chloramphenicol Includedi

n Modified β-Cyclodextrins. J. Korean Chem (31) 11.


This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License

Journal Pharmaceutical And Sciences (JPS) is published under the terms of the Creative Commons Attribution-Share. According to license under a Commons Attribution Non-Commercial ShareAlike 4.0

Authors retain the copyright to their work. Users can freely read, print, copy, and distribute material/works in any form, media, or format, provided the author and Journal Pharmaceutical And Sciences (JPS) are properly credited or cited. Authors are permitted to also store the published article at other websites or database systems.

Authors are permitted to distribute the published article to colleagues, post it to open websites - non-commercial, reuse of portions or extracts from the articles in other works, and text & data mining. However, authors are not allowed to send their manuscript to other scientific journals once it has been decided to be reviewed by reviewers at Journal Pharmaceutical And Sciences (JPS).

Downloads

Download data is not yet available.