Antioxidant Activities from Ethanol Extract, Hexane, Ethyl Acetate, and Water Fractions of Kapulaga Leaf (Elettaria cardamomum (L.) Maton)
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Keywords

Elettaria cardamomum
DPPH
Antioxidant

How to Cite

ASRA, R., Azni, N., Rusdi, R., & Nessa, N. (2019). Antioxidant Activities from Ethanol Extract, Hexane, Ethyl Acetate, and Water Fractions of Kapulaga Leaf (Elettaria cardamomum (L.) Maton). Journal of Pharmaceutical And Sciences, 2(1), 30-37. https://doi.org/10.36490/journal-jps.com.v2i1.17
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Abstract

This study aims to determine the antioxidant activity of the ethanol extract, hexane fraction, ethyl acetate fraction and water fraction of cardamom leaves (Elettaria cardamomum (L.) Maton). Dried of cardamom leaves was extracted by maceration using 70 % ethanol, then followed by fractionation based on the level of polarity with hexane, ethyl acetate, and water solvents. Antioxidant activity examination was determined using reagent DPPH using UV-Visible spectrophotometry and gallic acid as standard. The results of phytochemical screening showed that ethanol extract and water fraction of cardamom leaves contains phenol, flavonoids, tannins, and saponins, for ethyl acetate fraction contains phenol, flavonoids, and tannins, for hexane fraction the results were negative for all the compounds tested. The results of the antioxidant activity examination of ethanolic extract, hexane fraction, ethyl acetate fraction and water fraction of cardamom leaves showed the IC50 of 4058.7 μg/mL, 8419.3 μg/mL, 2281.7 μg/mL, and 3889.6 μg/mL respectively, whereas gallic acid showed the IC50 value of 4.89 μg/mL. Based on IC50 values ethanol extract, hexane fraction, ethyl acetate fraction and water fraction of cardamom leaves have weak antioxidant activity compared to gallic acid which has high antioxidant activity.

https://doi.org/10.36490/journal-jps.com.v2i1.17
PDF (Bahasa Indonesia)

References

Banu, K. S. & Cathrine, L. (2015). General technique involved in phytochemical analysis. International Journal of Advanced Research in Chemical Science (IJARCS). 2(4): 25-32.

Departemen Kesehatan Republik Indonesia. (1985). Cara Pembuatan simplisia. Jakarta : Departemen Kesehatan Republik Indonesia.

Departemen Kesehatan Republik Indonesia. (1995). Materia Medika Indonesia (Jilid VI). Jakarta: Direktorat Jenderal Pengawasan Obat dan Makanan.

Departemen Kesehatan Republik Indonesia. (2000). Parameter standar umum ekstrak tumbuhan obat. Jakarta: Direktorat Jenderal Pengawasan Obat dan Makanan.

Hanani, E. (2017). Analisis fitokimia. Jakarta: Buku kedokteran EGC.

Harborne. J.B. (1987). Metode fitokimia: penuntun cara modern menganalisis tumbuhan. Penerjemah: K. Padmawinata., & I. Soediro. Bandung: ITB.

Kementerian Kesehatan Republik Indonesia. (2010). Farmakope herbal Indonesia. (Suplemen I). Jakarta: Kementrian Kesehatan Indonesia.

Khatri, P., Rana, R. S., Jamdagni, P., & Sindhu, A. (2017). Phytochemical screening, GC-MS and FT-IR analysis of methanolic extract leaves of Elettaria cardamomum. International Journal of Research, 5(2), 213-224.

Molyneux, P. (2004). The use of the stable free radical diphenylpicrylhydrazyl (DPPH) for estimating antioxidant activity. Songklanakarin J. Sci. Technol. 26(2), 211-219.

Mosquera, O. M., Correa, Y. M. Buitrago, D. C., & Jaime, N. (2007). Antioxidant activity of twenty five plants from colombian biodiversity. Inst Oswaldo Cruz, Rio de janeiro, 102(5), 631-634

Peraturan Menteri Kesehatan Republik Indonesia. (2016). Formularium obat herbal asli Indonesia. Jakarta: Menteri Kesehatan Republik Indonesia.

Pourmorad, F., Hosseinimehr, S. J., & Shahabimajd, N. (2006). Antioxidant activity phenol and flavonoid content of some selected iranian medicinal plantsm. Africa Journal of Biotechnology, 5(110), 1142-1145.

Sari, P. E., Simanjuntak, S. B. I., & Winarsi, H. (2014). Aktivitas enzim superoksida dismutase tikus diabetes yang diberi ekstrak daun kapulaga (Amomum cardamomum). Jurnal Scripta Biologica, 1(3), 193-196.

Sayuti, K., & Yenrina, R. (2015). Antioksidan alami dan sintetik. (Cetakan 1). Padang: Andalas University Press

Silalahi, M. (2017). Bioaktivitas Amomum compactum Soland Ex Maton dan perspektif konservasinya. Jurnal Pro-Life, 4(2), 320-328.

Winarsi, H. (2007). Antioksidan alami dan radikal bebas. (Cetakan 1). Yogyakarta: Kanisius.

Winarsi, H., Sasongko, N. D., Purwanto, D., & Nuraini, I. (2013). Ekstrak daun kapulaga menurunkan indeks atherogenik dan kadar gula darah tikus diabetes induksi alloxan, Jurnal Agritech, 33(3), 273-280.


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