Antibacterial Activity Test of Ethanol Extract from Dried Simplicia Garlic (Allium sativum L.) Against Bacillus cereus Bacteria
PDF (Bahasa Indonesia)

Keywords

Dried simplisia, Garlic, Antibacterial, Bacillus cereus

How to Cite

Athaillah, A., & Lestari, U. (2020). Antibacterial Activity Test of Ethanol Extract from Dried Simplicia Garlic (Allium sativum L.) Against Bacillus cereus Bacteria. Journal of Pharmaceutical And Sciences, 3(2), 93-99. https://doi.org/10.36490/journal-jps.com.v3i2.51
Abstract viewed = 0 times
PDF (Bahasa Indonesia) downloaded = 0 times

Abstract

This research is aimed to find out the extract activity dried simplisia of garlic (Allium sativum L.) as an antibacterial which is capable to inhibiting growth of Bacillus cereus bacterium, to find out the alkaloid secondary of metabolites, flavonoid, tannin and saponin that contained in dried extract of garlic and to find out a concentration that has the most antibacterial activity which is capable to inhibiting growth of Bacillus cereus bacterium. Garlic was extracted by maceration method using a solvent ethanol 96%. After the extract obtaned, then phtochemical screening and standardization test. Thickening technique by evaporation used vacum rotary evaporator until thick extarct was obtained. Antibacterial activity thest by using disk diffusion method. This research was used seven concentrations which were 20% (b/v), 30% (b/v). 40% (b/v), 50% (b/v), 60% (b/v), 80% (b/v)and100% (b/v). The result of phytochemical screening test, ethanol extract garlic (Allium sativum L.) positive contained compound alkaloid, flavonoid, saponin and tannin. Standardization of power simplisia fulfill the requirements set by Depkes RI 2000. The result of activity test of optimum antibacterial was obtained inhibitory power with the number 27 mm on 40% (b/v) concentration. This proves that garlic (Allium sativum L.) have antibacterial affects against Bacillus cereus bacterium

https://doi.org/10.36490/journal-jps.com.v3i2.51
PDF (Bahasa Indonesia)

References

Akiyama, H., Fujii, K., Yamasaki, O., Oono, T., & Iwatsuki, K. (2001). Antibacterial action of several tannins against Staphylococcus aureus. Journal of Antimicrobial Chemotherapy, 48(4), 487–491. https://doi.org/10.1093/jac/48.4.487

Amanati, L. (2014). Uji Bakteri Staphylococcus Aureus Dan Bacillus Cereus Pada Produk Mi Instan Yang Beredar Di Pasaran ( Staphylococcus Aureus And Bacillus Cereus Bacteria Test On Instant Noodle Products At The Market ). Berita Litbang Industri, 3(2), 73–80.

Cushnie, T. P. T., & Lamb, A. J. (2005). Antimicrobial activity of flavonoids. International Journal of Antimicrobial Agents, 26(5), 343–356. https://doi.org/10.1016/j.ijantimicag.2005.09.002

Departeman Kesehatan RI. 2000. Materia Medika. Jilid. VI. Diktorat Jendral POM-Depkes RI. Jakarta.

Departemen Kesehatan RI. 2000. Parameter Standar Umum Ekstrak Tumbuhan Obat. Diktor Jendral POM-Depkes RI. Jakarta.

Ditjen POM. (1995). Farmakope indonesia Edisi ke IV. Jakarta: Departemen Kesehatan Republik Indonesia. Hal. 1061, 1066.

Difco, & Manual, B. (2009). Manual of Microbiological Culture Media (II; M. (ASCP) Mary Jo Zimbro, B.S., P. D. David A. Power, M. (ASCP) Sharon M. Miller, B.S., M. (ASCP) George E. Wilson, MBA, B.S., & B. A. Julie A. Johnson, eds.). Retrieved from http://galachem.ru/upload/iblock/c79/difcobblmanual_2nded.pdf

Harborne, J. B., Sudiro, I., Padmawinata, K., & Niksolihin, S. (1996). Metode fitokimia : penuntun cara modern menganalisis tumbuhan / J. B. Harborne ; diterjemahkan oleh Kosasih Padmawinata dan Iwang Soediro ; penyunting, Sofia Niksolihin. Retrieved from https://opac.perpusnas.go.id/DetailOpac.aspx?id=577450#

Kusumaningsih, A. (2010). Beberapa Bakteri Patogenik Penyebab Foodborne Disease pada Bahan Pangan Asal Ternak. Jurnal Wartazoa, 20(3), 103–111.

Lay, B. W. (1994). Analisis mikroba di laboratorium. PT. Raja Grafindo Persada. Jakarta, 168.

Lingga, M. E., & Rustama, M. M. (2006). UJI AKTIVITAS ANTIBAKTERI DARI EKSTRAK AIR DAN ETANOL BAWANG PUTIH (Allium sativum L.) TERHADAP BAKTERI GRAM NEGATIF DAN GRAM POSITIF YANG DIISOLASI DARI UDANG DOGOL (Metapenaeus monoceros), UDANG LOBSTER (Panulirus sp), DAN UDANG REBON (Mysis dan Acetes). BIOTIKA Jurnal Ilmiah Biologi, 5(2).

Noer, S., Pratiwi, R. D., & Gresinta, E. (2018). Penetapan Kadar Senyawa Fitokimia (Tanin, Saponin dan Flavonoid) sebagai Kuersetin Pada Ekstrak Daun Inggu (Ruta angustifolia L.). Jurnal Eksakta, 18(1), 19–29. https://doi.org/10.20885/eksakta.vol18.iss1.art3

Prihandani, S. S. (2015). UJI DAYA ANTIBAKTERI BAWANG PUTIH (Allium sativum L.) TERHADAP BAKTERI Staphylococcus aureus, Escherichia coli, Salmonella typhimurium DAN Pseudomonas aeruginosa DALAM MENINGKATKAN KEAMANAN PANGAN. Informatika Pertanian, 24(1), 53. https://doi.org/10.21082/ip.v24n1.2015.p53-58

Ross, Z. M., O’Gara, E. A., Hill, D. J., Sleightholme, H. V., & Maslin, D. J. (2001). Antimicrobial properties of garlic oil against human enteric bacteria: Evaluation of methodologies and comparisons with garlic oil sulfides and garlic powder. Applied and Environmental Microbiology, 67(1), 475–480. https://doi.org/10.1128/AEM.67.1.475-480.2001

Sabir, A. (2005). Aktivitas antibakteri flavonoid propolis Trigona sp terhadap bakteri Streptococcus mutans (in vitro) (In vitro antibacterial activity of flavonoids Trigona sp propolis against Streptococcus mutans). Dental Journal (Majalah Kedokteran Gigi), 38(3), 135. https://doi.org/10.20473/j.djmkg.v38.i3.p135-141

Syamsiah, I. S., & Tajudin. (2003). Khasiat & manfaat bawang putih: raja antibiotik alami. Retrieved from https://agromedia.net/katalog/khasiat-dan-manfaat-bawang-putih-raja-antibiotik-alami/
Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

Downloads

Download data is not yet available.